| 49 matches found in 28 documents. Search time: 0.187 seconds. |
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Score: 6.00 |
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| Title: The Metabolism of Gibberellin A20 to Gibberellin A1 by Tall and Dwarf Mutants of Oryza sativa and Arabidopsis thaliana .
| | Author: Kobayashi M Gaskin P Spray CR Phinney BO MacMillan J | | Journal: Citation: V : 106 ( 4 ) P : 1367-1372 Year: 1994 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12232414 Accession (PMID): 12232414 | | Abstract: The purpose of this study was to demonstrate the metabolism of gibberellin A20 ( GA20 ) to gibberellin A1 ( GA1 ) by tall and mutant shoots of rice ( Oryza sativa L ) and Arabidopsis thaliana ( L ) Heynh .
The data show that the tall and dx mutant of rice and the tall and ga5 mutant of Arabidopsis metabolize GA20 to GA1 .
The data also show that the dy mutant of rice and the ga4 mutant of Arabidopsis block the metabolism of GA20 to GA1 .
[ 17-13C , 3H ] GA20 was fed to tall and the dwarf mutants , dx and dy , of rice and tall and the dwarf mutants , ga5 and ga4 , of Arabidopsis .
The metabolites were analyzed by high-performance liquid chromatography and full-scan gas chromatography-mass spectrometry together with Kovats retention index data .
For rice , the metabolite [ 13C ] GA , was identified from tall and dx seedlings ; [ 13C ] GA1 was not identified from the dy seedlings .
[ 13C ] GA29 was identified from tall , dx , and dy seedlings .
For Arabidopsis , the metabolite [ 13C ] GA1 was identified from tall , ga5 , and ga4 plants .
The amount of [ 13C ] GA1 from ga4 plants was less than 15% of that obtained from tall and ga5 plants .
[ 13C ] GA29 was identified from tall , ga5 , and ga4 plants .
[ 13C ] GA5 and [ 13C ] GA3 were not identified from any of the six types of plant material | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The purpose of this study was to demonstrate the metabolism of gibberellin A20 ( GA20 ) to gibberellin A1 ( GA1 ) by tall and mutant shoots of rice ( Oryza sativa L ) and Arabidopsis thaliana ( L ) Heynh .
[ Sen. 2, subscore: 1.00 ]: The data show that the tall and dx mutant of rice and the tall and ga5 mutant of Arabidopsis metabolize GA20 to GA1 .
[ Sen. 3, subscore: 1.00 ]: The data also show that the dy mutant of rice and the ga4 mutant of Arabidopsis block the metabolism of GA20 to GA1 .
[ Sen. 6, subscore: 1.00 ]: For rice , the metabolite [ 13C ] GA , was identified from tall and dx seedlings ; [ 13C ] GA1 was not identified from the dy seedlings .
[ Sen. 8, subscore: 1.00 ]: For Arabidopsis , the metabolite [ 13C ] GA1 was identified from tall , ga5 , and ga4 plants .
[ Sen. 9, subscore: 1.00 ]: The amount of [ 13C ] GA1 from ga4 plants was less than 15% of that obtained from tall and ga5 plants .
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Score: 5.00 |
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| Title: Gibberellin biosynthesis : metabolic evidence for three steps in the early 13-hydroxylation pathway of rice .
| | Author: Kobayashi M MacMillan J Phinney B Gaskin P Spray CR Hedden P | | Journal: Phytochemistry Citation: V : 55 ( 4 ) P : 317-21 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11117879 Accession (PMID): 11117879 | | Abstract: [ 14C4 ] GA53 , [ 14C4 ] GA44 , and [ 2H2/14C4 ] GA19 were injected separately into seedlings of rice ( Oryza sativa ) using a dwarf mutant ( d35 ) that has low levels of endogenous gibberellins ( GAs ) .
After 8 h incubation , the shoots were extracted and the labeled metabolites were identified by full-scan gas chromatography mass spectrometry ( GC-MS ) and Kovats retention indices ( KRIs ) .
Our results document the metabolic sequence , GA53-->GA44-->GA19-->GA20 and the presence of endogenous GA53 , GA44 , GA19 , GA20 and GA1 .
Previous metabolic studies have shown the presence of the step , GA20-->GA1 in rice .
Taken together , the data establish in vegetative shoots of rice the presence of the early 13-hydroxylation pathway , a pathway that originates from GA12 and leads to bioactive GA1 . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Our results document the metabolic sequence , GA53-->GA44-->GA19-->GA20 and the presence of endogenous GA53 , GA44 , GA19 , GA20 and GA1 .
[ Sen. 5, subscore: 2.00 ]: Taken together , the data establish in vegetative shoots of rice the presence of the early 13-hydroxylation pathway , a pathway that originates from GA12 and leads to bioactive GA1 .
[ Sen. 1, subscore: 1.00 ]: [ 14C4 ] GA53 , [ 14C4 ] GA44 , and [ 2H2/14C4 ] GA19 were injected separately into seedlings of rice ( Oryza sativa ) using a dwarf mutant ( d35 ) that has low levels of endogenous gibberellins ( GAs ) .
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Score: 5.00 |
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| Title: Gibberellins and gravitropism in maize shoots : endogenous gibberellin-like substances and movement and metabolism of [ 3H ] Gibberellin A20 .
| | Author: Rood SB Kaufman PB Abe H Pharis RP .
| | Journal: Plant Physiol .
Citation: V : 83 ( ) P : 645-51 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11539033 Accession (PMID): 11539033 | | Abstract: [ 3H ] Gibberellin A20 ( GA20 ) of high specific radioactivity ( 49 . 9 gigabecquerel per millimole ) was applied equilaterally in a ring of microdrops to the internodal pulvinus of shoots of 3-week-old gravistimulated and vertical normal maize ( Zea mays L ) , and to a pleiogravitropic ( prostrate ) maize mutant , lazy ( la ) .
All plants converted the [ 3H ] GA20 to [ 3H ] GA1 and [ 3H ] GA29-like metabolites as well as to several metabolites with the partitioning and chromatographic behavior of glucosyl conjugates of [ 3H ] GA1 , [ 3H ] GA29 , and [ 3H ] GA8 .
The tentative identification of these putative [ 3H ] GA glucosyl conjugates was further supported by the release of the free [ 3H ] GA moiety after cleavage with cellulase .
Within 12 hours of the [ 3H ] GA20 feed , there was a significantly higher proportion of total radioactivity in lower than in upper halves of internode and leaf sheath pulvini in gravistimulated normal maize .
Further , there was a significantly higher proportion of putative free GA metabolites of [ 3H ] GA20 , especially [ 3H ] GA1 , in the lower halves of normal maize relative to upper halves .
The differential localization of the metabolites between upper and lower halves was not apparent in the pleiogravitropic mutant , la .
Endogenous GA-like substances were also examined in gravistimulated maize shoots .
Forty-eight hours after gravistimulation of 3-week-old maize seedlings , endogenous free GA-like substances in upper and lower leaf sheath and internode pulvini halves were extracted , chromatographed , and bioassayed using the "Tanginbozu" dwarf rice microdrop assay .
Lower halves contained consistently higher total levels of GA-like activity .
The qualitative elution profile of GA-like substances differed consistently , upper halves containing principally a GA20-like substance and lower halves containing principally a GA20-like substance and lower halves containing mainly GA1-like and GA19-like substances .
Gibberellins A1 ( 10 nanograms per gram ) and A20 ( 5 nanograms per gram ) were identified from these lower leaf sheath pulvini by capillary gas chromatography-selected ion monitoring .
Results from all of these experiments are consistent with a role for GAs in the differential shoot growth that follows gravitropism , although the results do not eliminate the possibility that the redistribution of GAs results from the gravitropic response .
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[ Sen. 2, subscore: 2.00 ]: All plants converted the [ 3H ] GA20 to [ 3H ] GA1 and [ 3H ] GA29-like metabolites as well as to several metabolites with the partitioning and chromatographic behavior of glucosyl conjugates of [ 3H ] GA1 , [ 3H ] GA29 , and [ 3H ] GA8 .
[ Sen. 10, subscore: 2.00 ]: The qualitative elution profile of GA-like substances differed consistently , upper halves containing principally a GA20-like substance and lower halves containing principally a GA20-like substance and lower halves containing mainly GA1-like and GA19-like substances .
[ Sen. 5, subscore: 1.00 ]: Further , there was a significantly higher proportion of putative free GA metabolites of [ 3H ] GA20 , especially [ 3H ] GA1 , in the lower halves of normal maize relative to upper halves .
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Score: 5.00 |
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| Title: Metabolism and Biological Activity of Gibberellin A4 in Vegetative Shoots of Zea mays , Oryza sativa , and Arabidopsis thaliana .
| | Author: Kobayashi M Gaskin P Spray CR Suzuki Y Phinney BO MacMillan J | | Journal: Citation: V : 102 ( 2 ) P : 379-386 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12231829 Accession (PMID): 12231829 | | Abstract: [ 17-13C , 3H ] Gibberellin A4 ( GA4 ) was injected into the shoots of tall ( W23/L317 ) , dwarf-1 ( d1 ) , and dwarf-5 ( d5 ) Zea mays L ( maize ) ; tall ( cv Nipponbare ) , dwarf-x ( dx ) , and dwarf-y ( dy ) Oryza sativa L ( rice ) ; and tall ( ecotype Landsberg erecta ) , ga4 , and ga5 Arabidopsis thaliana ( L ) Heynh .
[ 13C ] GA4 and its metabolites were identified from the shoots by full-scan gas chromatography-mass spectrometry and Kovats retention indices .
GA4 was metabolized to GA1 in all nine genotypes .
GA4 was also metabolized in some of the genotypes to 3-epi-GA1 , GA2 , 2 [ beta ] -OH-GA2 , 3-epi-GA2 , endo-GA4 , 16 [ alpha ] , 17-H2-16 , 17- ( OH ) 2-GA4 , GA34 , endo-GA34 , GA58 , 15-epi-GA63 , GA71 , and 16-epi-GA82 .
No evidence was found for the metabolism of GA4 to GA7 or of GA4 to GA3 .
The bioactivities of GA4 and GA1 were determined using the six dwarf mutants for assay .
GA4 and GA1 had similar activities for the maize and rice mutants .
For the Arabidopsis mutants , GA4 was more active than GA1 at low dosages ; GA4 was less active than GA1 at higher dosages . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: For the Arabidopsis mutants , GA4 was more active than GA1 at low dosages ; GA4 was less active than GA1 at higher dosages .
[ Sen. 3, subscore: 1.00 ]: GA4 was metabolized to GA1 in all nine genotypes .
[ Sen. 6, subscore: 1.00 ]: The bioactivities of GA4 and GA1 were determined using the six dwarf mutants for assay .
[ Sen. 7, subscore: 1.00 ]: GA4 and GA1 had similar activities for the maize and rice mutants .
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Score: 5.00 |
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| Title: Isolation of a gibberellin-producing fungus ( Penicillium sp .
MH7 ) and growth promotion of Crown daisy ( Chrysanthemum coronarium ) .
| | Author: Hamayun M Khan SA Iqbal I Ahmad B Lee IJ | | Journal: J Microbiol Biotechnol Citation: V : 20 P : 202-7 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20134253 Accession (PMID): 20134253 | | Abstract: Plant growth promoting fungi ( PGPF ) are well known for the production of useful secondary metabolites .
However , limited informations are available on gibberellin ( GA ) production capacity of PGPF of endophytic origin .
In current study , 15 fungal endophytes were isolated from the roots of Crown daisy , and then screened on Waito-c rice , in order to identify plant growth promoting fungi .
The fungal isolate MH7 significantly increased shoot length ( 12 . 1 cm ) of Waito-c in comparison to control treatment ( 7 . 9 cm ) .
In a separate experiment , culture filtrate ( CF ) of MH7 significantly promoted growth attributes of Crown daisy .
The MH7 CF was analyzed for gibberellins and it contained all physiologically active gibberellins ( GA1 , 1 . 37 ng/ml ; GA3 , 5 . 88 ng/ml ; GA4 , 8 . 62 ng/ml and GA7 , 2 . 05 ng/ml ) in conjunction with physiologically inactive GA9 ( 0 . 83 ng/ml ) , GA12 ( 0 . 44 ng/ml ) , GA15 ( 0 . 74 ng/ml ) , GA19 ( 1 . 16 ng/ml ) and GA20 ( 0 . 98 ng/ml ) .
The CF of MH7 produced higher amounts of GA3 , GA4 , GA7 , GA9 and GA12 than wild type Fusarium fujikuroi , which was used as control for GA production .
The fungal isolate MH7 was later identified as a new strain of Penicillium on the basis of morphological characteristics and phylogenetic analysis of 18S rDNA sequence .
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[ Sen. 6, subscore: 4.00 ]: The MH7 CF was analyzed for gibberellins and it contained all physiologically active gibberellins ( GA1 , 1 . 37 ng/ml ; GA3 , 5 . 88 ng/ml ; GA4 , 8 . 62 ng/ml and GA7 , 2 . 05 ng/ml ) in conjunction with physiologically inactive GA9 ( 0 . 83 ng/ml ) , GA12 ( 0 . 44 ng/ml ) , GA15 ( 0 . 74 ng/ml ) , GA19 ( 1 . 16 ng/ml ) and GA20 ( 0 . 98 ng/ml ) .
[ Sen. 7, subscore: 1.00 ]: The CF of MH7 produced higher amounts of GA3 , GA4 , GA7 , GA9 and GA12 than wild type Fusarium fujikuroi , which was used as control for GA production .
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