154 matches found in 101 documents. Search time: 0.15 seconds. |
|
Score: 10.00 | Title: The homeotic gene long sterile lemma ( G1 ) specifies sterile lemma identity in the rice spikelet .
| Author: Yoshida A Suzaki T Tanaka W Hirano HY | Journal: Proc Natl Acad Sci U S A Citation: V : 106 P : 20103-8 Year: 2009 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub19901325 Accession (PMID): 19901325 | Abstract: The mechanism of floral organ specification is principally conserved in angiosperms , as demonstrated by the ABC model .
By contrast , mechanisms that regulate the development of organs or structures specific to a group of species remain unclear .
Grasses have unique inflorescence units , comprising spikelets and florets .
In the genus Oryza ( rice ) , the single spikelet consists of a fertile floret subtended by a lemma and a palea , two sterile lemmas , and rudimentary glumes .
Each sterile lemma is a tiny glume-like organ with a smooth surface .
Here , we have examined a long sterile lemma1 ( g1 ) mutant , in which the sterile lemma is enlarged like the lemma .
Detailed phenotypic analysis reveals that the large sterile lemma in the g1 mutant appears to be caused by homeotic transformation of the sterile lemma into a lemma , suggesting that G1 is involved in the repression of lemma identity to specify the sterile lemma .
Gene isolation reveals that G1 is a member of a plant-specific gene family that encodes proteins with a previously uncharacterized domain , named here ALOG ( Arabidopsis LSH1 and Oryza G1 ) .
G1 mRNA is expressed in sterile lemma primordia throughout their development , and G1 protein is localized in the nucleus .
A trans-activation assay using the yeast GAL4 system suggests that G1 is involved in transcriptional regulation .
Repression of lemma identity by G1 is consistent with a hypothesis proposed to explain the morphological evolution of rice spikelets .
We also show that a wild rice species , Oryza grandiglumis , that forms large sterile lemmas has serious mutations in the G1 gene .
| Matching Sentences: [ Sen. 7, subscore: 2.00 ]: Detailed phenotypic analysis reveals that the large sterile lemma in the g1 mutant appears to be caused by homeotic transformation of the sterile lemma into a lemma , suggesting that G1 is involved in the repression of lemma identity to specify the sterile lemma . [ Sen. 8, subscore: 2.00 ]: Gene isolation reveals that G1 is a member of a plant-specific gene family that encodes proteins with a previously uncharacterized domain , named here ALOG ( Arabidopsis LSH1 and Oryza G1 ) . [ Sen. 9, subscore: 2.00 ]: G1 mRNA is expressed in sterile lemma primordia throughout their development , and G1 protein is localized in the nucleus . [ Sen. 6, subscore: 1.00 ]: Here , we have examined a long sterile lemma1 ( g1 ) mutant , in which the sterile lemma is enlarged like the lemma . [ Sen. 10, subscore: 1.00 ]: A trans-activation assay using the yeast GAL4 system suggests that G1 is involved in transcriptional regulation . [ Sen. 11, subscore: 1.00 ]: Repression of lemma identity by G1 is consistent with a hypothesis proposed to explain the morphological evolution of rice spikelets . [ Sen. 12, subscore: 1.00 ]: We also show that a wild rice species , Oryza grandiglumis , that forms large sterile lemmas has serious mutations in the G1 gene .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 | Title: Ammonium Uptake by Rice Roots ( I Fluxes and Subcellular Distribution of 13NH4+ ) .
| Author: Wang MY Siddiqi MY Ruth TJ Glass A | Journal: Citation: V : 103 ( 4 ) P : 1249-1258 Year: 1993 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12232017 Accession (PMID): 12232017 | Abstract: The time course of 13NH4+ uptake and the distribution of 13NH4+ among plant parts and subcellular compartments was determined for 3-week-old rice ( Oryza sativa L cv M202 ) plants grown hydroponically in modified Johnsons nutrient solution containing 2 , 100 , or 1000 [ mu ] M NH4+ ( referred to hereafter as G2 , G100 , or G1000 plants , respectively ) .
At steady state , the influx of 13NH4+ was determined to be 1 . 31 , 5 . 78 , and 10 . 11 [ mu ] mol g-1 fresh weight h-1 , respectively , for G2 , G100 , and G1000 plants ; efflux was 11 , 20 , and 29% , respectively , of influx .
The NH4+ flux to the vacuole was calculated to be between 1 and 1 . 4 [ mu ] mol g-1 fresh weight h-1 .
By means of 13NH4+ efflux analysis , three kinetically distinct phases ( superficial , cell wall , and cytoplasm ) were identified , with t1/2 for 13NH4+ exchange of approximately 3 s and 1 and 8 min , respectively .
Cytoplasmic [ NH4+ ] was estimated to be 3 . 72 , 20 . 55 , and 38 . 08 mM for G2 , G100 , and G1000 plants , respectively .
These concentrations were higher than vacuolar [ NH4+ ] , yet 72 to 92% of total root NH4+ was located in the vacuole .
Distributions of newly absorbed 13NH4+ between plant parts and among the compartments were also examined .
During a 30-min period G100 plants metabolized 19% of the influxed 13NH4+ .
The remainder ( 81% ) was partitioned among the vacuole ( 20% ) , cytoplasm ( 41% ) , and efflux ( 20% ) .
Of the metabolized 13N , roughly one-half was translocated to the shoots . | Matching Sentences: [ Sen. 1, subscore: 2.00 ]: The time course of 13NH4+ uptake and the distribution of 13NH4+ among plant parts and subcellular compartments was determined for 3-week-old rice ( Oryza sativa L cv M202 ) plants grown hydroponically in modified Johnsons nutrient solution containing 2 , 100 , or 1000 [ mu ] M NH4+ ( referred to hereafter as G2 , G100 , or G1000 plants , respectively ) . [ Sen. 2, subscore: 2.00 ]: At steady state , the influx of 13NH4+ was determined to be 1 . 31 , 5 . 78 , and 10 . 11 [ mu ] mol g-1 fresh weight h-1 , respectively , for G2 , G100 , and G1000 plants ; efflux was 11 , 20 , and 29% , respectively , of influx . [ Sen. 5, subscore: 2.00 ]: Cytoplasmic [ NH4+ ] was estimated to be 3 . 72 , 20 . 55 , and 38 . 08 mM for G2 , G100 , and G1000 plants , respectively . [ Sen. 8, subscore: 1.00 ]: During a 30-min period G100 plants metabolized 19% of the influxed 13NH4+ .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 | Title: Cytological identification of an isotetrasomic in rice and its application to centromere mapping .
| Author: Cheng ZK Yu HX Yan CJ Zhu LH Gu MH .
| Journal: Cell Res .
Citation: V : 7 ( 1 ) P : 31-8 Year: 1997 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub9261560 Accession (PMID): 9261560 | Abstract: The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map .
For obtaining these aneuploids in rice , the primary trisomics from triplo-1 to triplo-12 and the aneuploids derived from a triploid of indica rice variety Zhongxian 3037 were carefully investigated .
From the offsprings of triplo-10 , a primary trisomic of chromosome 10 of the variety , an isotetrasomic "triplo-10-1" was obtained .
Cytological investigation revealed that a pair of extra isochromosomes of triplo-10-1 were come from the short arm of chromosome 10 .
In the offsprings of the isotetrasomic , a secondary trisomic "triplo-10-2" , in which the extra chromosome was an isochromosome derived from the short arm of chromosome 10 , was identified .
With the isotetrasomic , secondary trisomic , primary trisomic and diploid of variety Zhongxian 3037 , different molecular markers were used for exploring the position of the centromere of chromosome 10 .
Based on the DNA dosage effect , it was verified that the molecular markers G1125 , G333 and L169 were located on the short arm , G1084 and other 16 available molecular markers were on the long arm of chromosome 10 .
So the centromere of chromosome 10 was located somewhere between G1125 and G1084 according to the RFLP linkage map given by Kurata et al [ 1 ] .
The distance from G1125 to G1084 was about 3 . 2 cM . | Matching Sentences: [ Sen. 7, subscore: 2.00 ]: Based on the DNA dosage effect , it was verified that the molecular markers G1125 , G333 and L169 were located on the short arm , G1084 and other 16 available molecular markers were on the long arm of chromosome 10 . [ Sen. 8, subscore: 2.00 ]: So the centromere of chromosome 10 was located somewhere between G1125 and G1084 according to the RFLP linkage map given by Kurata et al [ 1 ] . [ Sen. 9, subscore: 2.00 ]: The distance from G1125 to G1084 was about 3 . 2 cM .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 | Title: Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells .
| Author: Obata T Kitamoto HK Nakamura A Fukuda A Tanaka Y | Journal: Plant Physiol Citation: V : 144 P : 1978-85 Year: 2007 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub17586689 Accession (PMID): 17586689 | Abstract: We screened a rice ( Oryza sativa L Nipponbare ) full-length cDNA expression library through functional complementation in yeast ( Saccharomyces cerevisiae ) to find novel cation transporters involved in salt tolerance .
We found that expression of a cDNA clone , encoding the rice homolog of Shaker family K ( + ) channel KAT1 ( OsKAT1 ) , suppressed the salt-sensitive phenotype of yeast strain G19 ( Deltaena1-4 ) , which lacks a major component of Na ( + ) efflux .
It also suppressed a K ( + ) -transport-defective phenotype of yeast strain CY162 ( Deltatrk1Deltatrk2 ) , suggesting the enhancement of K ( + ) uptake by OsKAT1 .
By the expression of OsKAT1 , the K ( + ) contents of salt-stressed G19 cells increased during the exponential growth phase .
At the linear phase , however , OsKAT1-expressing G19 cells accumulated less Na ( + ) than nonexpressing cells , but almost the same K ( + ) .
The cellular Na ( + ) to K ( + ) ratio of OsKAT1-expressing G19 cells remained lower than nonexpressing cells under saline conditions .
Rice cells overexpressing OsKAT1 also showed enhanced salt tolerance and increased cellular K ( + ) content .
These functions of OsKAT1 are likely to be common among Shaker K ( + ) channels because OsAKT1 and Arabidopsis ( Arabidopsis thaliana ) KAT1 were able to complement the salt-sensitive phenotype of G19 as well as OsKAT1 .
The expression of OsKAT1 was restricted to internodes and rachides of wild-type rice , whereas other Shaker family genes were expressed in various organs .
These results suggest that OsKAT1 is involved in salt tolerance of rice in cooperation with other K ( + ) channels by participating in maintenance of cytosolic cation homeostasis during salt stress and thus protects cells from Na ( + ) .
| Matching Sentences: [ Sen. 2, subscore: 1.00 ]: We found that expression of a cDNA clone , encoding the rice homolog of Shaker family K ( + ) channel KAT1 ( OsKAT1 ) , suppressed the salt-sensitive phenotype of yeast strain G19 ( Deltaena1-4 ) , which lacks a major component of Na ( + ) efflux . [ Sen. 4, subscore: 1.00 ]: By the expression of OsKAT1 , the K ( + ) contents of salt-stressed G19 cells increased during the exponential growth phase . [ Sen. 5, subscore: 1.00 ]: At the linear phase , however , OsKAT1-expressing G19 cells accumulated less Na ( + ) than nonexpressing cells , but almost the same K ( + ) . [ Sen. 6, subscore: 1.00 ]: The cellular Na ( + ) to K ( + ) ratio of OsKAT1-expressing G19 cells remained lower than nonexpressing cells under saline conditions . [ Sen. 8, subscore: 1.00 ]: These functions of OsKAT1 are likely to be common among Shaker K ( + ) channels because OsAKT1 and Arabidopsis ( Arabidopsis thaliana ) KAT1 were able to complement the salt-sensitive phenotype of G19 as well as OsKAT1 .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 | Title: Molecular cloning of the gene for plant proliferating-cell nuclear antigen and expression of this gene during the cell cycle in synchronized cultures of Catharanthus roseus cells .
| Author: Kodama H Ito M Ohnishi N Suzuka I Komamine A | Journal: Eur .
J Biochem .
Citation: V : 197 ( 2 ) P : 495-503 Year: 1991 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub1902790 Accession (PMID): 1902790 | Abstract: A cDNA library was screened for plant proliferating-cell nuclear antigen ( PCNA ) from Catharanthus roseus ( periwinkle ) .
A lambda gt11 cDNA library was constructed using poly ( A ) -rich RNA isolated from the cells in the S phase .
A cDNA clone for PCNA was isolated by using a rice genomic clone , pCJ-1 , which contains PCNA-related gene sequences .
The cDNA contains an open reading frame of 804 nucleotides , encoding a protein of 268 amino acids with a molecular mass of 29 , 765 Da .
When conservative substitutions were included , a high degree of similarity ( about 85% ) was observed between the predicted amino acid sequence of periwinkle PCNA and that of human PCNA .
Expression of mRNA for periwinkle PCNA was undetectable or very weak in quiescent cells , such as phosphate-starved cells , auxin-starved cells and cells in the stationary phase .
In the synchronous progression of the cell cycle induced by the addition of phosphate or auxin , the active accumulation of periwinkle PCNA mRNA was observed preferentially in the S phase .
When an inhibitor of DNA synthesis , aphidicolin , was added to the cells at the G1 phase , an increase in the level of PCNA mRNA was observed .
The partial inhibition of protein synthesis at the G1 phase by a protein inhibitor , anisomycin , caused the arrest of cells in the G1 phase .
No increase of the level of periwinkle PCNA mRNA was observed in cells arrested at the G1 phase by the inhibition of protein synthesis .
These results indicate that the induction of mRNA for periwinkle PCNA occurred independently of the initiation of DNA replication , but that synthesis of certain proteins at the G1 phase was required for the induction of periwinkle PCNA mRNA at the S phase .
| Matching Sentences: [ Sen. 9, subscore: 2.00 ]: The partial inhibition of protein synthesis at the G1 phase by a protein inhibitor , anisomycin , caused the arrest of cells in the G1 phase . [ Sen. 8, subscore: 1.00 ]: When an inhibitor of DNA synthesis , aphidicolin , was added to the cells at the G1 phase , an increase in the level of PCNA mRNA was observed . [ Sen. 10, subscore: 1.00 ]: No increase of the level of periwinkle PCNA mRNA was observed in cells arrested at the G1 phase by the inhibition of protein synthesis . [ Sen. 11, subscore: 1.00 ]: These results indicate that the induction of mRNA for periwinkle PCNA occurred independently of the initiation of DNA replication , but that synthesis of certain proteins at the G1 phase was required for the induction of periwinkle PCNA mRNA at the S phase .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |