| 13 matches found in 4 documents. Search time: 0.137 seconds. |
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Score: 8.00 |
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| Title: Formate dehydrogenase in rice plant : growth stimulation effect of formate in rice plant .
| | Author: Shiraishi T Fukusaki E Kobayashi A | | Journal: J Biosci . Bioeng . Citation: V : 89 ( 3 ) P : 241-6 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16232736 Accession (PMID): 16232736 | | Abstract: NAD-dependent formate dehydrogenase ( FDH , EC 1 . 2 . 1 . 2 . ) plays a key role in the final step of the catabolism of methanol in methylotrophs .
In certain plant species , this enzyme is a mitochondrial and NAD-dependent enzyme .
In this study , the complete cDNA sequence of the rice ( Oryza sativa cv Hinohikari ) FDH gene , which contains an open reading frame ( ORF ) of 1128 bp , was determined .
The sequence corresponds to a protein of 376 amino acids and shows strong homology to the NAD-dependent FDHs from other plants .
Our experimental results showed that the expression of the rice cDNA in Escherichia coli induced FDH activity , indicating that the cDNA encodes a functional peptide of FDH .
The application of formate to the roots of rice seedlings leads to growth increment both in terms of the length of the aerial part and the fresh weight .
The FDH activity and the accumulation of FDH transcripts were strongly enhanced in the root portions of the formate-fed plants .
This enhancement indicates that the transcriptional regulation which responds to formate functions in the rice plant , and that formate can act as an effective inducer for FDH activity . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Our experimental results showed that the expression of the rice cDNA in Escherichia coli induced FDH activity , indicating that the cDNA encodes a functional peptide of FDH .
[ Sen. 7, subscore: 2.00 ]: The FDH activity and the accumulation of FDH transcripts were strongly enhanced in the root portions of the formate-fed plants .
[ Sen. 1, subscore: 1.00 ]: NAD-dependent formate dehydrogenase ( FDH , EC 1 . 2 . 1 . 2 . ) plays a key role in the final step of the catabolism of methanol in methylotrophs .
[ Sen. 3, subscore: 1.00 ]: In this study , the complete cDNA sequence of the rice ( Oryza sativa cv Hinohikari ) FDH gene , which contains an open reading frame ( ORF ) of 1128 bp , was determined .
[ Sen. 4, subscore: 1.00 ]: The sequence corresponds to a protein of 376 amino acids and shows strong homology to the NAD-dependent FDHs from other plants .
[ Sen. 8, subscore: 1.00 ]: This enhancement indicates that the transcriptional regulation which responds to formate functions in the rice plant , and that formate can act as an effective inducer for FDH activity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry .
| | Author: Shiraishi T Fukusaki E Kajiyama S Kobayashi A | | Journal: Citation: V : 855 ( 1 ) P : 337-40 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10514998 Accession (PMID): 10514998 | | Abstract: A new method using GC-MS was devised for the convenient measurement of formate dehydrogenase ( FDH ) activity in crude it issue samples .
FDH activity was detected by measuring headspace 13CO2 , which was enzymically converted from [ 13C ] formic acid .
This method proved to be sensitive and simple for the estimation of FDH activity without complicated pretreatment . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A new method using GC-MS was devised for the convenient measurement of formate dehydrogenase ( FDH ) activity in crude it issue samples .
[ Sen. 2, subscore: 1.00 ]: FDH activity was detected by measuring headspace 13CO2 , which was enzymically converted from [ 13C ] formic acid .
[ Sen. 3, subscore: 1.00 ]: This method proved to be sensitive and simple for the estimation of FDH activity without complicated pretreatment .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: CFL1 , a WW domain protein , regulates cuticle development by modulating the function of HDG1 , a class IV homeodomain transcription factor , in rice and Arabidopsis .
| | Author: Wu R Li S He S Wassmann F Yu C Qin G Schreiber L Qu LJ Gu H | | Journal: Plant Cell Citation: V : 23 P : 3392-411 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21954461 Accession (PMID): 21954461 | | Abstract: Plants have a chemically heterogeneous lipophilic layer , the cuticle , which protects them from biotic and abiotic stresses .
The mechanisms that regulate cuticle development are poorly understood .
We identified a rice ( Oryza sativa ) dominant curly leaf mutant , curly flag leaf1 ( cfl1 ) , and cloned CFL1 , which encodes a WW domain protein .
We overexpressed both rice and Arabidopsis CFL1 in Arabidopsis thaliana ; these transgenic plants showed severely impaired cuticle development , similar to that in cfl1 rice .
Reduced expression of At CFL1 resulted in reinforcement of cuticle structure .
At CFL1 was predominantly expressed in specialized epidermal cells and in regions where dehiscence and abscission occur .
Biochemical evidence showed that At CFL1 interacts with HDG1 , a class IV homeodomain-leucine zipper transcription factor .
Suppression of HDG1 function resulted in similar defective cuticle phenotypes in wild-type Arabidopsis but much alleviated phenotypes in At cfl1-1 mutants .
The expression of two cuticle development-associated genes , BDG and FDH , was downregulated in At CFL1 overexpressor and HDG1 suppression plants .
HDG1 binds to the cis-element L1 box , which exists in the regulatory regions of BDG and FDH .
Our results suggest that rice and Arabidopsis CFL1 negatively regulate cuticle development by affecting the function of HDG1 , which regulates the downstream genes BDG and FDH .
| Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: The expression of two cuticle development-associated genes , BDG and FDH , was downregulated in At CFL1 overexpressor and HDG1 suppression plants .
[ Sen. 10, subscore: 1.00 ]: HDG1 binds to the cis-element L1 box , which exists in the regulatory regions of BDG and FDH .
[ Sen. 11, subscore: 1.00 ]: Our results suggest that rice and Arabidopsis CFL1 negatively regulate cuticle development by affecting the function of HDG1 , which regulates the downstream genes BDG and FDH .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Expression profiling of Oryza sativa metal homeostasis genes in different rice cultivars using a cDNA macroarray .
| | Author: Narayanan NN Vasconcelos MW Grusak MA | | Journal: Plant Physiol Biochem Citation: V : 45 P : 277-86 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17468002 Accession (PMID): 17468002 | | Abstract: Rice is an important food crop , but it is a poor source of essential micronutrients such as iron and zinc .
In order to improve the metal ion content of rice grains through breeding or biotechnology , more information is needed on the molecular players that help mobilize metals from leaves to developing seeds .
To profile several genes simultaneously , a cDNA macroarray was developed using 36 metal-related genes from rice , including ZIPs , NRAMPs , and YSLs ( coding for known or potential metal transporters ) , as well as NAS , FER , FRO , NAAT , FDH , GSTU , and PDR ( involved in metal homeostasis ) .
Because flag leaves are the major source of phloem-delivered photoassimilates and remobilized metals for developing seeds , we analyzed the expression of these metal-related genes in flag and non-flag leaves of four different rice cultivars ( Cocodrie , Taipei 309 , IR58 , and IR68144 ) during the period of mid-grain fill .
Genes ( 24 of 36 ) exhibited low to non-detectable signals in the macroarray , while 12 genes ( OsIRT1 , OsZIP1 , OsZIP5 , OsZIP8 , OsYSL5 , OsYSL6 , OsYSL7 , OsYSL8 , OsYSL18 , OsNRAMP2 , OsNRAMP4 and OsNRAMP7 ) were found to be highly expressed in both flag and non-flag leaves of all four cultivars .
Additional expression analysis using semi-quantitative or quantitative PCR provided results that were generally consistent with the macroarray , but semi-quantitative PCR confirmed that OsFDH , OsFER1 , OsNAAT , OsNAS1 , OsPDR9 , OsYSL12 , OsYSL13 , OsZIP7 , and OsZIP10 were also expressed in leaves .
This specialized macroarray has provided a short list of potential candidate genes , expressed in leaves , which might contribute to the process of metal transport to distant sinks , such as seeds .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: To profile several genes simultaneously , a cDNA macroarray was developed using 36 metal-related genes from rice , including ZIPs , NRAMPs , and YSLs ( coding for known or potential metal transporters ) , as well as NAS , FER , FRO , NAAT , FDH , GSTU , and PDR ( involved in metal homeostasis ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
