| 18 matches found in 6 documents. Search time: 0.186 seconds. |
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| Title: EUI1 , encoding a putative cytochrome P450 monooxygenase , regulates internode elongation by modulating gibberellin responses in rice .
| | Author: Luo A Qian Q Yin H Liu X Yin C Lan Y Tang J Tang Z Cao S Wang X Xia K Fu X Luo D Chu C | | Journal: Plant Cell Physiol .
Citation: V : 47 ( 2 ) P : 181-91 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16306061 Accession (PMID): 16306061 | | Abstract: Elongation of rice internodes is one of the most important agronomic traits , which determines the plant height and underlies the grain yield .
It has been shown that the elongation of internodes is under genetic control , and various factors are implicated in the process .
Here , we report a detailed characterization of an elongated uppermost internode1 ( eui1 ) mutant , which has been used in hybrid rice breeding .
In the eui1-2 mutant , the cell lengths in the uppermost internodes are significantly longer than that of wild type and thus give rise to the elongated uppermost internode .
It was found that the level of active gibberellin was elevated in the mutant , whereas its growth in response to gibberellin is similar to that of the wild type , suggesting that the higher level accumulation of gibberellin in the eui1 mutant causes the abnormal elongation of the uppermost internode .
Consistently , the expression levels of several genes which encode gibberellin biosynthesis enzymes were altered .
We cloned the EUI1 gene , which encodes a putative cytochrome P450 monooxygenase , by map-based cloning and found that EUI1 was weakly expressed in most it issues , but preferentially in young panicles .
To confirm its function , transgenic experiments with different constructs of EUI1 were conducted .
Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes , which could be partially reversed by supplementation with gibberellin .
Furthermore , apart from the alteration of expression levels of the gibberellin biosynthesis genes , accumulation of SLR1 protein was found in the overexpressing transgenic plants , indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis .
Therefore , we proposed that EUI1 plays a negative role in gibberellin-mediated regulation of cell elongation in the uppermost internode of rice . | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: We cloned the EUI1 gene , which encodes a putative cytochrome P450 monooxygenase , by map-based cloning and found that EUI1 was weakly expressed in most it issues , but preferentially in young panicles .
[ Sen. 3, subscore: 1.00 ]: Here , we report a detailed characterization of an elongated uppermost internode1 ( eui1 ) mutant , which has been used in hybrid rice breeding .
[ Sen. 4, subscore: 1.00 ]: In the eui1-2 mutant , the cell lengths in the uppermost internodes are significantly longer than that of wild type and thus give rise to the elongated uppermost internode .
[ Sen. 5, subscore: 1.00 ]: It was found that the level of active gibberellin was elevated in the mutant , whereas its growth in response to gibberellin is similar to that of the wild type , suggesting that the higher level accumulation of gibberellin in the eui1 mutant causes the abnormal elongation of the uppermost internode .
[ Sen. 8, subscore: 1.00 ]: To confirm its function , transgenic experiments with different constructs of EUI1 were conducted .
[ Sen. 9, subscore: 1.00 ]: Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes , which could be partially reversed by supplementation with gibberellin .
[ Sen. 10, subscore: 1.00 ]: Furthermore , apart from the alteration of expression levels of the gibberellin biosynthesis genes , accumulation of SLR1 protein was found in the overexpressing transgenic plants , indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis .
[ Sen. 11, subscore: 1.00 ]: Therefore , we proposed that EUI1 plays a negative role in gibberellin-mediated regulation of cell elongation in the uppermost internode of rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
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| Title: Fine mapping and in silico isolation of the EUI1 gene controlling upper internode elongation in rice .
| | Author: Ma H Zhang S Ji L Zhu H Yang S Fang X Yang R | | Journal: Plant Mol . Biol . Citation: V : 60 ( 1 ) P : 87-94 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16463101 Accession (PMID): 16463101 | | Abstract: Upper internode elongation in rice is an important agronomic trait .
Well-known mutants with an elongated uppermost internode ( eui ) are important germplasms for developing unsheathed-panicle male-sterile lines in hybrid rice breeding .
We finely mapped the eui1 gene and identified its candidate gene using in silico analysis based on previous research work and rice genomic sequence data .
The rice eui1 gene was mapped to two overlapping BAC clones , OSJNBa0095J22 and OSJNBb0099O15 , between the markers AC40 and AC46 , that were 0 . 64 cM apart and spanned approximately 152 kb .
A simple sequence repeat ( SSR ) marker AC41 that cosegregated with eui1 was located in an intron of a putative cytochrome P450-related gene .
In silico analysis suggested that this encoded the cytochrome CYP714D1 .
Allelic sequencing confirmed that EUI1 corresponded to this P450 gene .
A gamma ray-induced eui1 mutant carried a deletion in exon II of the EUI1 gene , and resulted in a frame-shift deletion that produced a truncated polypeptide .
We conclude that the EUI1 gene controlling the upper internode elongation in rice is 9 , 804 bp long , and comprises two exons and one intron .
The length of the cDNA is 1 , 931 bp containing a 1 , 734 bp ORF , a 110 bp 5-UTR and a 87 bp 3-UTR .
The ORF encodes an unknown 577 amino acid functional protein , that appears to be a member of the cytochrome P450 family . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: A gamma ray-induced eui1 mutant carried a deletion in exon II of the EUI1 gene , and resulted in a frame-shift deletion that produced a truncated polypeptide .
[ Sen. 3, subscore: 1.00 ]: We finely mapped the eui1 gene and identified its candidate gene using in silico analysis based on previous research work and rice genomic sequence data .
[ Sen. 4, subscore: 1.00 ]: The rice eui1 gene was mapped to two overlapping BAC clones , OSJNBa0095J22 and OSJNBb0099O15 , between the markers AC40 and AC46 , that were 0 . 64 cM apart and spanned approximately 152 kb .
[ Sen. 5, subscore: 1.00 ]: A simple sequence repeat ( SSR ) marker AC41 that cosegregated with eui1 was located in an intron of a putative cytochrome P450-related gene .
[ Sen. 7, subscore: 1.00 ]: Allelic sequencing confirmed that EUI1 corresponded to this P450 gene .
[ Sen. 9, subscore: 1.00 ]: We conclude that the EUI1 gene controlling the upper internode elongation in rice is 9 , 804 bp long , and comprises two exons and one intron .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Two Arabidopsis cytochrome P450 monooxygenases , CYP714A1 and CYP714A2 , function redundantly in plant development through gibberellin deactivation .
| | Author: Zhang Y Zhang B Yan D Dong W Yang W Li Q Zeng L Wang J Wang L Hicks LM He Z | | Journal: Plant J Citation: V : 67 P : 342-53 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21457373 Accession (PMID): 21457373 | | Abstract: The rice gene ELONGATED UPPERMOST INTERNODE1 ( EUI1 ) encodes a P450 monooxygenase that epoxidizes gibberellins ( GAs ) in a deactivation reaction .
The Arabidopsis genome contains a tandemly duplicated gene pair ELA1 ( CYP714A1 ) and ELA2 ( CYP714A2 ) that encode EUI homologs .
In this work , we dissected the functions of the two proteins .
ELA1 and ELA2 exhibited overlapping yet distinct gene expression patterns .
We showed that while single mutants of ELA1 or ELA2 exhibited no obvious morphological phenotype , simultaneous elimination of ELA1 and ELA2 expression in ELA1-RNAi/ela2 resulted in increased biomass and enlarged organs .
By contrast , transgenic plants constitutively expressing either ELA1 or ELA2 were dwarfed , similar to those overexpressing the rice EUI gene .
We also discovered that overexpression of ELA1 resulted in a severe dwarf phenotype , while overexpression of ELA2 gave rise to a breeding-favored semi-dwarf phenotype in rice .
Consistent with the phenotypes , we found that the ELA1-RNAi/ela2 plants increased amounts of biologically active GAs that were decreased in the internodes of transgenic rice with ELA1 and ELA2 overexpression .
In contrast , the precursor GA ( 12 ) slightly accumulated in the transgenic rice , and GA ( 19 ) highly accumulated in the ELA2 overexpression rice .
Taken together , our study strongly suggests that the two Arabidopsis EUI homologs subtly regulate plant growth most likely through catalyzing deactivation of bioactive GAs similar to rice EUI .
The two P450s may also function in early stages of the GA biosynthetic pathway .
Our results also suggest that ELA2 could be an excellent tool for molecular breeding for high yield potential in cereal crops .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice gene ELONGATED UPPERMOST INTERNODE1 ( EUI1 ) encodes a P450 monooxygenase that epoxidizes gibberellins ( GAs ) in a deactivation reaction .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: [ Fine localization of rice EUI1 gene controlling elongation of the uppermost internode ] | | Author: Wang RX Li PJ Chen HQ Wen SK Li JY Zhu XD .
| | Journal: Yi Chuan Xue Bao Citation: V : 32 ( 9 ) P : 955-9 Year: 2005 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16201240 Accession (PMID): 16201240 | | Abstract: The height of rice is one of the important agricultural traits , which affects on the rice architecture and production directly .
The investigation of the length of the uppermost internode showed that the elongation mutation of the first internode is controlled by a recessive nuclear locus .
The gene is localized between the STS marker of E30531 and the CAPS marker of C903 on the long arm of chromosome 5 , apart from 6 . 7 cM and 2 . 8 cM respectively .
The fine localization maps the gene between 0 . 3 cM flanks , which will help clone this gene and study the mechanism of the gene function .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: [ Fine localization of rice EUI1 gene controlling elongation of the uppermost internode ]
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: EUI1 , encoding a putative cytochrome P450 monooxygenase , regulates internode elongation by modulating gibberellin responses in rice .
| | Author: Luo A Qian Q Yin H Liu X Yin C Lan Y Tang J Tang Z Cao S Wang X Xia K Fu X Luo D Chu C | | Journal: Plant Cell Physiol .
Citation: V : 47 ( 2 ) P : 181-91 Year: 2006 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16306061 Accession (PMID): 16306061 | | Abstract: Elongation of rice internodes is one of the most important agronomic traits , which determines the plant height and underlies the grain yield .
It has been shown that the elongation of internodes is under genetic control , and various factors are implicated in the process .
Here , we report a detailed characterization of an elongated uppermost internode1 ( eui1 ) mutant , which has been used in hybrid rice breeding .
In the eui1-2 mutant , the cell lengths in the uppermost internodes are significantly longer than that of wild type and thus give rise to the elongated uppermost internode .
It was found that the level of active gibberellin was elevated in the mutant , whereas its growth in response to gibberellin is similar to that of the wild type , suggesting that the higher level accumulation of gibberellin in the eui1 mutant causes the abnormal elongation of the uppermost internode .
Consistently , the expression levels of several genes which encode gibberellin biosynthesis enzymes were altered .
We cloned the EUI1 gene , which encodes a putative cytochrome P450 monooxygenase , by map-based cloning and found that EUI1 was weakly expressed in most it issues , but preferentially in young panicles .
To confirm its function , transgenic experiments with different constructs of EUI1 were conducted .
Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes , which could be partially reversed by supplementation with gibberellin .
Furthermore , apart from the alteration of expression levels of the gibberellin biosynthesis genes , accumulation of SLR1 protein was found in the overexpressing transgenic plants , indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis .
Therefore , we proposed that EUI1 plays a negative role in gibberellin-mediated regulation of cell elongation in the uppermost internode of rice . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: EUI1 , encoding a putative cytochrome P450 monooxygenase , regulates internode elongation by modulating gibberellin responses in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
