Score: 6.00 |
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| Title: Isolation and characterization of a water-stress-inducible cDNA clone from Solanum chacoense .
| | Author: Silhavy D Hutvgner G Barta E Bnfalvi Z | | Journal: Plant Mol . Biol . Citation: V : 27 ( 3 ) P : 587-95 Year: 1995 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub7894021 Accession (PMID): 7894021 | | Abstract: A rich source of valuable genes are wild species .
Solanum chacoense Bitter with its extreme resistance to viruses , insects and drought , is a good example .
In the present study , a stress gene , designated DS2 , has been isolated from S chacoense .
We have shown that the expression of the gene is organ-specific being detected in leaf , stem and stolon , but not in root , tuber or flower .
Treatment of detached leaves with abscisic acid ( ABA ) , salicylic acid or methyl jasmonate resulted in only very moderate accumulation of DS2 mRNA .
Thus , DS2 represents a very rare type of the water-stress-inducible genes whose signalling pathway is not primarily related to ABA .
Based on DNA sequence analysis , DS2 encodes a putative protein starting with 20 amino acids homologous to the ABA and water-stress-inducible , ripening-related ( ASR ) proteins of tomato continued by an insert of 155 amino acids structurally similar to certain LEAs ( late embryogenesis-abundant proteins ) and ending in 88 amino acids homologous again to the ASR sequences and to an unpublished partial cDNA fragment isolated from the root of rice .
The N-terminal region of the DS2 protein is hydrophilic with ten 13-mer amino acid motifs and random coil structure .
In contrast , the C-terminus predicts an alpha-helix and possesses a bipartite nuclear targeting sequence motif .
These data suggest that the function of the DS2 may be the protection of the nuclear DNA from desiccation .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: In the present study , a stress gene , designated DS2 , has been isolated from S chacoense .
[ Sen. 5, subscore: 1.00 ]: Treatment of detached leaves with abscisic acid ( ABA ) , salicylic acid or methyl jasmonate resulted in only very moderate accumulation of DS2 mRNA .
[ Sen. 6, subscore: 1.00 ]: Thus , DS2 represents a very rare type of the water-stress-inducible genes whose signalling pathway is not primarily related to ABA .
[ Sen. 7, subscore: 1.00 ]: Based on DNA sequence analysis , DS2 encodes a putative protein starting with 20 amino acids homologous to the ABA and water-stress-inducible , ripening-related ( ASR ) proteins of tomato continued by an insert of 155 amino acids structurally similar to certain LEAs ( late embryogenesis-abundant proteins ) and ending in 88 amino acids homologous again to the ASR sequences and to an unpublished partial cDNA fragment isolated from the root of rice .
[ Sen. 8, subscore: 1.00 ]: The N-terminal region of the DS2 protein is hydrophilic with ten 13-mer amino acid motifs and random coil structure .
[ Sen. 10, subscore: 1.00 ]: These data suggest that the function of the DS2 may be the protection of the nuclear DNA from desiccation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: qDTY1 . 1 , a major QTL for rice grain yield under reproductive-stage drought stress with a consistent effect in multiple elite genetic backgrounds .
| | Author: Vikram P Swamy BM Dixit S Ahmed HU Teresa Sta Cruz M Singh AK Kumar A | | Journal: BMC Genet Citation: V : 12 P : 89 Year: 2011 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22008150 Accession (PMID): 22008150 | | Abstract: ABSTRACT : BACKGROUND : Drought is one of the most important abiotic stresses causing drastic reductions in yield in rainfed rice environments .
The suitability of grain yield ( GY ) under drought as a selection criterion has been reported in the past few years .
Most of the quantitative trait loci ( QTLs ) for GY under drought in rice reported so far has been in the background of low-yielding susceptible varieties .
Such QTLs have not shown a similar effect in multiple high yielding drought-susceptible varieties , thus limiting their use in marker-assisted selection .
Genetic control of GY under reproductive-stage drought stress ( RS ) in elite genetic backgrounds was studied in three F3 : 4 mapping populations derived from crosses of N22 , a drought-tolerant aus cultivar , with Swarna , IR64 , and MTU1010 , three high-yielding popular mega-varieties , with the aim to identify QTLs for GY under RS that show a consistent effect in multiple elite genetic backgrounds .
Three populations were phenotyped under RS in the dry seasons ( DS ) of 2009 and 2010 at IRRI .
For genotyping , whole-genome scans for N22/MTU1010 and bulked segregant analysis for N22/Swarna and N22/IR64 were employed using SSR markers .
RESULTS : A major QTL for GY under RS , qDTY1 . 1 , was identified on rice chromosome 1 flanked by RM11943 and RM431 in all three populations .
In combined analysis over two years , qDTY1 . 1 showed an additive effect of 29 . 3% , 24 . 3% , and 16 . 1% of mean yield in N22/Swarna , N22/IR64 , and N22/MTU1010 , respectively , under RS . qDTY1 . 1 also showed a positive effect on GY in non-stress ( NS ) situations in N22/Swarna , N22/IR64 over both years , and N22/MTU1010 in DS2009 .
CONCLUSIONS : This is the first reported QTL in rice with a major and consistent effect in multiple elite genetic backgrounds under both RS and NS situations .
Consistency of the QTL effect across different genetic backgrounds makes it a suitable candidate for use in marker-assisted breeding .
| Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: In combined analysis over two years , qDTY1 . 1 showed an additive effect of 29 . 3% , 24 . 3% , and 16 . 1% of mean yield in N22/Swarna , N22/IR64 , and N22/MTU1010 , respectively , under RS . qDTY1 . 1 also showed a positive effect on GY in non-stress ( NS ) situations in N22/Swarna , N22/IR64 over both years , and N22/MTU1010 in DS2009 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
