Score: 6.00 |
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| Title: Dro1 , a major QTL involved in deep rooting of rice under upland field conditions .
| | Author: Uga Y Okuno K Yano M | | Journal: J Exp Bot Citation: V : 62 P : 2485-94 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21212298 Accession (PMID): 21212298 | | Abstract: Developing a deep root system is an important strategy for avoiding drought stress in rice .
Using the basket method , the ratio of deep rooting ( RDR ; the proportion of total roots that elongated through the basket bottom ) was calculated to evaluate deep rooting .
A new major quantitative trait locus ( QTL ) controlling RDR was detected on chromosome 9 by using 117 recombinant inbred lines ( RILs ) derived from a cross between the lowland cultivar IR64 , with shallow rooting , and the upland cultivar Kinandang Patong ( KP ) , with deep rooting .
This QTL explained 66 . 6% of the total phenotypic variance in RDR in the RILs .
A BC ( 2 ) F ( 3 ) line homozygous for the KP allele of the QTL had an RDR of 40 . 4% , compared with 2 . 6% for the homozygous IR64 allele .
Fine mapping of this QTL was undertaken using eight BC ( 2 ) F ( 3 ) recombinant lines .
The RDR QTL Dro1 ( Deeper rooting 1 ) was mapped between the markers RM24393 and RM7424 , which delimit a 608 . 4 kb interval in the reference cultivar Nipponbare .
To clarify the influence of Dro1 in an upland field , the root distribution in different soil layers was quantified by means of core sampling .
A line homozygous for the KP allele of Dro1 ( Dro1-KP ) and IR64 did not differ in root dry weight in the shallow soil layers ( 0-25 cm ) , but root dry weight of Dro1-KP in deep soil layers ( 25-50 cm ) was significantly greater than that of IR64 , suggesting that Dro1 plays a crucial role in increased deep rooting under upland field conditions .
| Matching Sentences:
[ Sen. 9, subscore: 4.00 ]: A line homozygous for the KP allele of Dro1 ( Dro1-KP ) and IR64 did not differ in root dry weight in the shallow soil layers ( 0-25 cm ) , but root dry weight of Dro1-KP in deep soil layers ( 25-50 cm ) was significantly greater than that of IR64 , suggesting that Dro1 plays a crucial role in increased deep rooting under upland field conditions .
[ Sen. 7, subscore: 1.00 ]: The RDR QTL Dro1 ( Deeper rooting 1 ) was mapped between the markers RM24393 and RM7424 , which delimit a 608 . 4 kb interval in the reference cultivar Nipponbare .
[ Sen. 8, subscore: 1.00 ]: To clarify the influence of Dro1 in an upland field , the root distribution in different soil layers was quantified by means of core sampling .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Dro1 , a major QTL involved in deep rooting of rice under upland field conditions .
| | Author: Uga Y Okuno K Yano M | | Journal: J Exp Bot Citation: V : 62 P : 2485-94 Year: 2011 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub21212298 Accession (PMID): 21212298 | | Abstract: Developing a deep root system is an important strategy for avoiding drought stress in rice .
Using the basket method , the ratio of deep rooting ( RDR ; the proportion of total roots that elongated through the basket bottom ) was calculated to evaluate deep rooting .
A new major quantitative trait locus ( QTL ) controlling RDR was detected on chromosome 9 by using 117 recombinant inbred lines ( RILs ) derived from a cross between the lowland cultivar IR64 , with shallow rooting , and the upland cultivar Kinandang Patong ( KP ) , with deep rooting .
This QTL explained 66 . 6% of the total phenotypic variance in RDR in the RILs .
A BC ( 2 ) F ( 3 ) line homozygous for the KP allele of the QTL had an RDR of 40 . 4% , compared with 2 . 6% for the homozygous IR64 allele .
Fine mapping of this QTL was undertaken using eight BC ( 2 ) F ( 3 ) recombinant lines .
The RDR QTL Dro1 ( Deeper rooting 1 ) was mapped between the markers RM24393 and RM7424 , which delimit a 608 . 4 kb interval in the reference cultivar Nipponbare .
To clarify the influence of Dro1 in an upland field , the root distribution in different soil layers was quantified by means of core sampling .
A line homozygous for the KP allele of Dro1 ( Dro1-KP ) and IR64 did not differ in root dry weight in the shallow soil layers ( 0-25 cm ) , but root dry weight of Dro1-KP in deep soil layers ( 25-50 cm ) was significantly greater than that of IR64 , suggesting that Dro1 plays a crucial role in increased deep rooting under upland field conditions .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Dro1 , a major QTL involved in deep rooting of rice under upland field conditions .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
