| 6 matches found in 3 documents. Search time: 0.023 seconds. |
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Score: 4.00 |
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| Title: Overexpression of Arabidopsis and rice stress genes inducible transcription factor confers drought and salinity tolerance to rice .
| | Author: Datta K Baisakh N Ganguly M Krishnan S Yamaguchi Shinozaki K Datta SK | | Journal: Plant Biotechnol J Citation: V : 10 P : 579-86 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22385556 Accession (PMID): 22385556 | | Abstract: Rice yield is greatly affected by environmental stresses such as drought and salinity .
In response to the challenge of producing rice plants tolerant to these stresses , we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter .
Two different indica rice cultivars were used , BR29 , an improved commercially cultivated variety from Bangladesh and IR68899B , an IRRI bred maintainer line for hybrid rice .
Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice , whereas biolistic transformation was done with rice DREB1B in the case of IR68899B .
Initial genetic integration was confirmed by PCR and Southern blot analysis .
Salinity tolerance was assayed in very young seedlings .
Drought stress tests were found to be more reliable when they were carried out at the pre-flowering booting stage .
RNA gel blot analysis as well as quantitative PCR analysis was performed to estimate the transcription level under stressed and unstressed conditions .
Agronomic performance studies were done with stressed and unstressed plants to compare the yield losses due to dehydration and salt loading stresses .
Noticeably enhanced tolerance to dehydration was observed in the plants transformed with DREB1A isolated from Arabidopsis while DREB1B was found to be more effective for salt tolerance .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice , whereas biolistic transformation was done with rice DREB1B in the case of IR68899B .
[ Sen. 2, subscore: 1.00 ]: In response to the challenge of producing rice plants tolerant to these stresses , we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter .
[ Sen. 10, subscore: 1.00 ]: Noticeably enhanced tolerance to dehydration was observed in the plants transformed with DREB1A isolated from Arabidopsis while DREB1B was found to be more effective for salt tolerance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Rice DREB1B promoter shows distinct stress-specific responses , and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance .
| | Author: Gutha LR Reddy AR | | Journal: Plant Mol Biol Citation: V : 68 P : 533-55 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18754079 Accession (PMID): 18754079 | | Abstract: CBF/DREB ( C-repeat binding factor/dehydration responsive element binding factor ) family of transcription factors in plants is reported to be associated with regulation of gene expression under stress conditions .
Here , we report the functional characterization of a DREB transcription factor , DREB1B gene from rice ( Oryza sativa ssp . indica ) .
The OsDREB1B gene was differentially regulated at the transcriptional level by osmotic stress , oxidative stress , salicylic acid , ABA , and cold .
A 745 bp promoter region of OsDREB1B cDNA was fused to the beta-glucuronidase ( GUS ) gene and introduced via Agrobacterium tumifaciens into the genome of Arabidopsis .
Histochemical analysis of GUS expression in T ( 2 ) transgenic Arabidopsis plants indicated that OsDREB1B shows stress-specific induction pattern in response to a variety of stresses like mannitol , NaCl , PEG , methyl viologen , cold , ABA , and salicylic acid .
Leaf-order-dependent induction pattern of the promoter was observed in response to both cold and ABA stresses .
Further , OsDREB1B cDNA was introduced into tobacco plants under the control of CaMV35S promoter to investigate the role of DREB1B product in plant stress response .
Transgenic tobacco plants have shown improved seed germination , root growth , membrane stability , and 2 , 2-diphenyl-1-pycrilhydrazil hydrate ( DPPH ) free radical scavenging activity under inhibitory concentrations of mannitol .
Importantly , transgenic plants accumulated higher fresh weight under long-term osmotic stress , and also have shown retention of more water than the wild type during drought stress .
Overexpression of OsDREB1B in tobacco also improved the oxidative and freezing stress tolerance of transgenic plants .
In addition , tobacco plants constitutively expressing OsDREB1B have shown decreased sensitivity to tobacco streak virus infection .
Constitutive expression of OsDREB1B in tobacco also induced the expression of PR genes in transgenic plants .
The data obtained provide strong in vivo evidence that OsDREB1B is involved in both abiotic and biotic stress responses , and confers broad-spectrum stress tolerance to transgenic plants .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Here , we report the functional characterization of a DREB transcription factor , DREB1B gene from rice ( Oryza sativa ssp . indica ) .
[ Sen. 7, subscore: 1.00 ]: Further , OsDREB1B cDNA was introduced into tobacco plants under the control of CaMV35S promoter to investigate the role of DREB1B product in plant stress response .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Rice DREB1B promoter shows distinct stress-specific responses , and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance .
| | Author: Gutha LR Reddy AR | | Journal: Plant Mol Biol Citation: V : 68 P : 533-55 Year: 2008 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18754079 Accession (PMID): 18754079 | | Abstract: CBF/DREB ( C-repeat binding factor/dehydration responsive element binding factor ) family of transcription factors in plants is reported to be associated with regulation of gene expression under stress conditions .
Here , we report the functional characterization of a DREB transcription factor , DREB1B gene from rice ( Oryza sativa ssp . indica ) .
The OsDREB1B gene was differentially regulated at the transcriptional level by osmotic stress , oxidative stress , salicylic acid , ABA , and cold .
A 745 bp promoter region of OsDREB1B cDNA was fused to the beta-glucuronidase ( GUS ) gene and introduced via Agrobacterium tumifaciens into the genome of Arabidopsis .
Histochemical analysis of GUS expression in T ( 2 ) transgenic Arabidopsis plants indicated that OsDREB1B shows stress-specific induction pattern in response to a variety of stresses like mannitol , NaCl , PEG , methyl viologen , cold , ABA , and salicylic acid .
Leaf-order-dependent induction pattern of the promoter was observed in response to both cold and ABA stresses .
Further , OsDREB1B cDNA was introduced into tobacco plants under the control of CaMV35S promoter to investigate the role of DREB1B product in plant stress response .
Transgenic tobacco plants have shown improved seed germination , root growth , membrane stability , and 2 , 2-diphenyl-1-pycrilhydrazil hydrate ( DPPH ) free radical scavenging activity under inhibitory concentrations of mannitol .
Importantly , transgenic plants accumulated higher fresh weight under long-term osmotic stress , and also have shown retention of more water than the wild type during drought stress .
Overexpression of OsDREB1B in tobacco also improved the oxidative and freezing stress tolerance of transgenic plants .
In addition , tobacco plants constitutively expressing OsDREB1B have shown decreased sensitivity to tobacco streak virus infection .
Constitutive expression of OsDREB1B in tobacco also induced the expression of PR genes in transgenic plants .
The data obtained provide strong in vivo evidence that OsDREB1B is involved in both abiotic and biotic stress responses , and confers broad-spectrum stress tolerance to transgenic plants .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice DREB1B promoter shows distinct stress-specific responses , and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
