| 9 matches found in 3 documents. Search time: 0.208 seconds. |
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| Title: An AT-hook gene is required for palea formation and floral organ number control in rice .
| | Author: Jin Y Luo Q Tong H Wang A Cheng Z Tang J Li D Zhao X Li X Wan J Jiao Y Chu C Zhu L | | Journal: Dev Biol Citation: V : 359 P : 277-88 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21924254 Accession (PMID): 21924254 | | Abstract: Grasses have highly specialized flowers and their outer floral organ identity remains unclear .
In this study , we identified and characterized rice mutants that specifically disrupted the development of palea , one of the outer whorl floral organs .
The depressed palea1 ( dp1 ) mutants show a primary defect in the main structure of palea , implying that palea is a fusion between the main structure and marginal it issues on both sides .
The sterile lemma at the palea side is occasionally elongated in dp1 mutants .
In addition , we found a floral organ number increase in dp1 mutants at low penetration .
Both the sterile lemma elongation and the floral organ number increase phenotype are enhanced by the mutation of an independent gene SMALL DEGENERATIVE PALEA1 ( SDP1 ) , whose single mutation causes reduced palea size .
E function and presumable A function floral homeotic genes were found suppressed in the dp1-2 mutant .
We identified the DP1 gene by map-based cloning and found it encodes a nuclear-localized AT-hook DNA binding protein , suggesting a grass-specific role of chromatin architecture modification in flower development .
The DP1 enhancer SDP1 was also positional cloned , and was found identical to the recently reported RETARDED PALEA1 ( REP1 ) gene encoding a TCP family transcription factor .
We further found that SDP1/REP1 is downstreamly regulated by DP1 .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The depressed palea1 ( dp1 ) mutants show a primary defect in the main structure of palea , implying that palea is a fusion between the main structure and marginal it issues on both sides .
[ Sen. 4, subscore: 1.00 ]: The sterile lemma at the palea side is occasionally elongated in dp1 mutants .
[ Sen. 5, subscore: 1.00 ]: In addition , we found a floral organ number increase in dp1 mutants at low penetration .
[ Sen. 7, subscore: 1.00 ]: E function and presumable A function floral homeotic genes were found suppressed in the dp1-2 mutant .
[ Sen. 8, subscore: 1.00 ]: We identified the DP1 gene by map-based cloning and found it encodes a nuclear-localized AT-hook DNA binding protein , suggesting a grass-specific role of chromatin architecture modification in flower development .
[ Sen. 9, subscore: 1.00 ]: The DP1 enhancer SDP1 was also positional cloned , and was found identical to the recently reported RETARDED PALEA1 ( REP1 ) gene encoding a TCP family transcription factor .
[ Sen. 10, subscore: 1.00 ]: We further found that SDP1/REP1 is downstreamly regulated by DP1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance .
| | Author: Kawakami A Sato Y Yoshida M | | Journal: J Exp Bot Citation: V : 59 P : 793-802 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18319240 Accession (PMID): 18319240 | | Abstract: Fructans are water-soluble fructose oligomers and polymers that are based on sucrose , and have been implicated in protecting plants against water stress .
Rice ( Oryza sativa L ) is highly sensitive to chilling temperatures , and is not able to synthesize fructans .
Two wheat fructan-synthesizing enzymes , sucrose : sucrose 1-fructosyltransferase , encoded by wft2 , or sucrose : fructan 6-fructosyltransferase , encoded by wft1 , were introduced into rice plants , and rice transformants that accumulate fructans were successfully obtained .
The mature leaf blades of transgenic rice lines with wft2 or wft1 accumulated 16 . 2 mg g ( -1 ) FW of oligo and polysaccharides mainly composed of inulin oligomers of more than DP7 , and 3 . 7 mg g ( -1 ) FW of oligo and polysaccharides , mainly composed of phlein oligomers of more than DP15 , respectively .
The transgenic rice seedlings with wft2 accumulated significantly higher concentrations of oligo and polysaccharides than non-transgenic rice seedlings , and exhibited enhanced chilling tolerance .
The oligo and polysaccharide concentrations of seedlings expressing wft1 were obviously lower than those of lines expressing wft2 , and no correlation between oligo and polysaccharide concentrations and chilling tolerance was detected in wft1-expressing rice lines .
The results suggest that transgenic rice lines expressing wheat-derived fructosyltransferase genes accumulated large amounts of fructans in mature leaf blades and exhibited enhanced chilling tolerance at the seedling stage .
This is the first report owing that fructan accumulation enhanced tolerance to non-freezing low temperatures .
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[ Sen. 4, subscore: 1.00 ]: The mature leaf blades of transgenic rice lines with wft2 or wft1 accumulated 16 . 2 mg g ( -1 ) FW of oligo and polysaccharides mainly composed of inulin oligomers of more than DP7 , and 3 . 7 mg g ( -1 ) FW of oligo and polysaccharides , mainly composed of phlein oligomers of more than DP15 , respectively .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Characterization of the reactions of starch branching enzymes from rice endosperm .
| | Author: Nakamura Y Utsumi Y Sawada T Aihara S Utsumi C Yoshida M Kitamura S | | Journal: Plant Cell Physiol Citation: V : 51 P : 776-94 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20305271 Accession (PMID): 20305271 | | Abstract: To our knowledge the present paper shows for the first time the kinetic parameters of all the three starch branching enzyme ( BE ) isozymes , BEI , BEIIa and BEIIb , from rice with both amylopectin and synthetic amylose as glucan substrate .
The activities of these BE isozymes with a linear glucan amylose decreased with a decrease in the molar size of amylose , and no activities of BEIIa and BEIIb were found when the degree of polymerization ( DP ) of amylose was lower than at least 80 , whereas BEI had an activity with amylose of a DP higher than approximately 50 .
Detailed analyses of debranched products from BE reactions revealed the distinct chain length preferences of the individual BE isozymes .
BEIIb almost exclusively transferred chains of DP7 and DP6 while BEIIa formed a wide range of short chains of DP6 to around DP15 from outer chains of amylopectin and amylose .
On the other hand , BEI formed a variety of short chains and intermediate chains of a DP | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: BEIIb almost exclusively transferred chains of DP7 and DP6 while BEIIa formed a wide range of short chains of DP6 to around DP15 from outer chains of amylopectin and amylose .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
