| 28 matches found in 12 documents. Search time: 0.102 seconds. |
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Score: 10.00 |
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| Title: Morphological alteration caused by brassinosteroid insensitivity increases the biomass and grain production of rice .
| | Author: Morinaka Y Sakamoto T Inukai Y Agetsuma M Kitano H Ashikari M Matsuoka M | | Journal: Plant Physiol .
Citation: V : 141 ( 3 ) P : 924-31 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16714407 Accession (PMID): 16714407 | | Abstract: The rice ( Oryza sativa ) dwarf mutant d61 phenotype is caused by loss of function of a rice BRASSINOSTEROID INSENSITIVE1 ortholog , OsBRI1 .
We have identified nine d61 alleles , the weakest of which , d61-7 , confers agronomically important traits such as semidwarf stature and erect leaves .
Because erect-leaf habit is considered to increase light capture for photosynthesis , we compared the biomass and grain production of wild-type and d61-7 rice .
The biomass of wild type was 38% higher than that of d61-7 at harvest under conventional planting density because of the dwarfism of d61-7 .
However , the biomass of d61-7 was 35% higher than that of wild type at high planting density .
The grain yield of wild type reached a maximum at middensity , but the yield of d61-7 continued to increase with planting density .
These results indicate that d61-7 produces biomass more effectively than wild type , and consequently more effectively assimilates the biomass in reproductive organ development at high planting density .
However , the small grain size of d61-7 counters any increase in grain yield , leading to the same grain yield as that of wild type even at high density .
We therefore produced transgenic rice with partial suppression of endogenous OsBRI1 expression to obtain the erect-leaf phenotype without grain changes .
The estimated grain yield of these transformants was about 30% higher than that of wild type at high density .
These results demonstrate the feasibility of generating erect-leaf plants by modifying the expression of the brassinosteroid receptor gene in transgenic rice plants . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: We have identified nine d61 alleles , the weakest of which , d61-7 , confers agronomically important traits such as semidwarf stature and erect leaves .
[ Sen. 4, subscore: 2.00 ]: The biomass of wild type was 38% higher than that of d61-7 at harvest under conventional planting density because of the dwarfism of d61-7 .
[ Sen. 1, subscore: 1.00 ]: The rice ( Oryza sativa ) dwarf mutant d61 phenotype is caused by loss of function of a rice BRASSINOSTEROID INSENSITIVE1 ortholog , OsBRI1 .
[ Sen. 3, subscore: 1.00 ]: Because erect-leaf habit is considered to increase light capture for photosynthesis , we compared the biomass and grain production of wild-type and d61-7 rice .
[ Sen. 5, subscore: 1.00 ]: However , the biomass of d61-7 was 35% higher than that of wild type at high planting density .
[ Sen. 6, subscore: 1.00 ]: The grain yield of wild type reached a maximum at middensity , but the yield of d61-7 continued to increase with planting density .
[ Sen. 7, subscore: 1.00 ]: These results indicate that d61-7 produces biomass more effectively than wild type , and consequently more effectively assimilates the biomass in reproductive organ development at high planting density .
[ Sen. 8, subscore: 1.00 ]: However , the small grain size of d61-7 counters any increase in grain yield , leading to the same grain yield as that of wild type even at high density .
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Score: 5.00 |
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| Title: Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint .
| | Author: Yamamuro C Ihara Y Wu X Noguchi T Fujioka S Takatsuto S Ashikari M Kitano H Matsuoka M | | Journal: Plant Cell Citation: V : 12 ( 9 ) P : 1591-606 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11006334 Accession (PMID): 11006334 | | Abstract: Brassinosteroids ( BRs ) are plant growth-promoting natural products required for plant growth and development .
Physiological studies have demonstrated that exogenous BR , alone or in combination with auxin , enhance bending of the lamina joint of rice .
However , little is known about the function of endogenous BR in rice or other grass species .
We report here the phenotypical and molecular characterization of a rice dwarf mutant , d61 , that is less sensitive to BR compared to the wild type .
We cloned a rice gene , OsBRI1 , with extensive sequence similarity to that of the Arabidopsis BRI gene , which encodes a putative BR receptor kinase .
Linkage analysis showed that the OsBRI1 gene is closely linked to the d61 locus .
Single nucleotide substitutions found at different sites of the d61 alleles would give rise to amino acid changes in the corresponding polypeptides .
Furthermore , introduction of the entire OsBRI1 coding region , including the 5 and 3 flanking sequences , into d61 plants complemented the mutation to display the wild-type phenotype .
Transgenic plants carrying the antisense strand of the OsBRI1 transcript showed similar or even more severe phenotypes than those of the d61 mutants .
Our results show that OsBRI1 functions in various growth and developmental processes in rice , including ( 1 ) internode elongation , by inducing the formation of the intercalary meristem and the longitudinal elongation of internode cells ; ( 2 ) bending of the lamina joint ; and ( 3 ) skotomorphogenesis . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: We report here the phenotypical and molecular characterization of a rice dwarf mutant , d61 , that is less sensitive to BR compared to the wild type .
[ Sen. 6, subscore: 1.00 ]: Linkage analysis showed that the OsBRI1 gene is closely linked to the d61 locus .
[ Sen. 7, subscore: 1.00 ]: Single nucleotide substitutions found at different sites of the d61 alleles would give rise to amino acid changes in the corresponding polypeptides .
[ Sen. 8, subscore: 1.00 ]: Furthermore , introduction of the entire OsBRI1 coding region , including the 5 and 3 flanking sequences , into d61 plants complemented the mutation to display the wild-type phenotype .
[ Sen. 9, subscore: 1.00 ]: Transgenic plants carrying the antisense strand of the OsBRI1 transcript showed similar or even more severe phenotypes than those of the d61 mutants .
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Score: 4.00 |
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| Title: The role of OsBRI1 and its homologous genes , OsBRL1 and OsBRL3 , in rice .
| | Author: Nakamura A Fujioka S Sunohara H Kamiya N Hong Z Inukai Y Miura K Takatsuto S Yoshida S Ueguchi-Tanaka M Hasegawa Y Kitano H Matsuoka M | | Journal: Plant Physiol .
Citation: V : 140 ( 2 ) P : 580-90 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16407447 Accession (PMID): 16407447 | | Abstract: Since first identifying two alleles of a rice ( Oryza sativa ) brassinosteroid ( BR ) -insensitive mutant , d61 , that were also defective in an orthologous gene in Arabidopsis ( Arabidopsis thaliana ) BRASSINOSTEROID INSENSITIVE1 ( BRI1 ) , we have isolated eight additional alleles , including null mutations , of the rice BRI1 gene OsBRI1 .
The most severe mutant , d61-4 , exhibited severe dwarfism and twisted leaves , although pattern formation and differentiation were normal This severe shoot phenotype was caused mainly by a defect in cell elongation and the disturbance of cell division after the determination of cell fate .
In contrast to its severe shoot phenotype , the d61-4 mutant had a mild root phenotype .
Concomitantly , the accumulation of castasterone , the active BR in rice , was up to 30-fold greater in the shoots , while only 1 . 5-fold greater in the roots .
The homologous genes for OsBRI1 , OsBRL1 and OsBRL3 , were highly expressed in roots but weakly expressed in shoots , and their expression was higher in d61-4 than in the wild type .
Based on these observations , we conclude that OsBRI1 is not essential for pattern formation or organ initiation , but is involved in organ development through controlling cell division and elongation .
In addition , OsBRL1 and OsBRL3 are at least partly involved in BR perception in the roots . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Since first identifying two alleles of a rice ( Oryza sativa ) brassinosteroid ( BR ) -insensitive mutant , d61 , that were also defective in an orthologous gene in Arabidopsis ( Arabidopsis thaliana ) BRASSINOSTEROID INSENSITIVE1 ( BRI1 ) , we have isolated eight additional alleles , including null mutations , of the rice BRI1 gene OsBRI1 .
[ Sen. 2, subscore: 1.00 ]: The most severe mutant , d61-4 , exhibited severe dwarfism and twisted leaves , although pattern formation and differentiation were normal This severe shoot phenotype was caused mainly by a defect in cell elongation and the disturbance of cell division after the determination of cell fate .
[ Sen. 3, subscore: 1.00 ]: In contrast to its severe shoot phenotype , the d61-4 mutant had a mild root phenotype .
[ Sen. 5, subscore: 1.00 ]: The homologous genes for OsBRI1 , OsBRL1 and OsBRL3 , were highly expressed in roots but weakly expressed in shoots , and their expression was higher in d61-4 than in the wild type .
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Score: 3.00 |
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| Title: Function of alpha subunit of heterotrimeric G protein in brassinosteroid response of rice plants .
| | Author: Oki K Kitagawa K Fujisawa Y Kato H Iwasaki Y | | Journal: Plant Signal Behav Citation: V : 4 P : 126-8 Year: 2009 Type: PubMed-not-MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19649188 Accession (PMID): 19649188 | | Abstract: The alpha subunit of heterotrimeric G-proteins ( G alpha ) is involved in a broad range of aspects of the brassinosteroid ( BR ) response , such as the enhancement of lamina bending .
However , it has been suggested from epistatic analysis of d1 and d61 , which are mutants deficient for G alpha and the BR receptor BRI1 , that G alpha and BRI1 may function via distinct pathways in many cases .
In this study , we investigated further the genetic interaction between G alpha and BRI1 .
We report the analysis of transformants of T65d1 and T65d1/d61-7 into which were introduced a constitutively active form of G alpha , Q223L .
The application of 24-epi-brassinolide ( 24-epiBL ) to T65d1 expressing Q223L still resulted in elongation of the coleoptile and , in fact , it was enhanced over the wild-type plant ( WT ) level in a concentration dependent manner .
In T65d1/d61-7 expressing Q223L , the seed size was enlarged over that of d61-7 due to activation of G alpha .
These results suggest that Q223L is able to augment the BR response in response to 24-epiBL and also that Q223L functions independently of BRI1 in the process of determining seed morphology , given that Q223L was functional in the BRI1-deficient mutant , d61-7 .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: However , it has been suggested from epistatic analysis of d1 and d61 , which are mutants deficient for G alpha and the BR receptor BRI1 , that G alpha and BRI1 may function via distinct pathways in many cases .
[ Sen. 6, subscore: 1.00 ]: In T65d1/d61-7 expressing Q223L , the seed size was enlarged over that of d61-7 due to activation of G alpha .
[ Sen. 7, subscore: 1.00 ]: These results suggest that Q223L is able to augment the BR response in response to 24-epiBL and also that Q223L functions independently of BRI1 in the process of determining seed morphology , given that Q223L was functional in the BRI1-deficient mutant , d61-7 .
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Score: 2.00 |
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| Title: DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice .
| | Author: Tong H Liu L Jin Y Du L Yin Y Qian Q Zhu L Chu C | | Journal: Plant Cell Citation: V : 24 P : 2562-77 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22685166 Accession (PMID): 22685166 | | Abstract: In Arabidopsis thaliana , the GSK3/SHAGGY-like kinase BRASSINOSTEROID-INSENSITIVE2 ( BIN2 ) plays a critical role in the brassinosteroid ( BR ) signaling pathway by negatively regulating the activities of bri1-EMS-SUPPRESSOR1/BRASSINAZOLE-RESISTANT1 family transcription factors that regulate the expression of downstream BR-responsive genes .
In this study , we analyzed the function of a rice ( Oryza sativa ) GSK3/SHAGGY-like kinase ( GSK2 ) , which is one of the orthologs of BIN2 .
Overexpression of GSK2 ( Go ) led to plants with typical BR loss-of-function phenotypes , and suppression of GSK2 resulted in enhanced BR signaling phenotypes .
DWARF AND LOW-TILLERING ( DLT ) is a positive regulator that mediates several BR responses in rice .
Suppression of DLT can enhance the phenotypes of BR receptor mutant d61-1 , and overexpression of DLT obviously suppressed the BR loss-of-function phenotypes of both d61-1 and Go , suggesting that DLT functions downstream of GSK2 to modulate BR responses .
Indeed , GSK2 can interact with DLT and phosphorylate DLT .
Moreover , brassinolide treatment can induce the dephosphorylation of DLT , leading to the accumulation of dephosphorylated DLT protein .
In GSK2 transgenic plants , the DLT phosphorylation level is dictated by the GSK2 level .
These results demonstrate that DLT is a GSK2 substrate , further reinforcing that the BIN2/GSK2 kinase has multiple substrates that carry out various BR responses .
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[ Sen. 5, subscore: 2.00 ]: Suppression of DLT can enhance the phenotypes of BR receptor mutant d61-1 , and overexpression of DLT obviously suppressed the BR loss-of-function phenotypes of both d61-1 and Go , suggesting that DLT functions downstream of GSK2 to modulate BR responses .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
