| 50 matches found in 22 documents. Search time: 0.061 seconds. |
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Score: 9.00 |
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| Title: A rice promoter containing both novel positive and negative cis-elements for regulation of green it issue-specific gene expression in transgenic plants .
| | Author: Cai M Wei J Li X Xu C Wang S | | Journal: Plant Biotechnol J Citation: V : 5 P : 664-74 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17596180 Accession (PMID): 17596180 | | Abstract: The it issue-specific expression of transgenes is essential in plant breeding programmes to avoid the fitness costs caused by constitutive expression of a target gene .
However , knowledge on the molecular mechanisms of it issue-specific gene expression and practicable it issue-specific promoters is limited .
In this study , we identified the cis-acting elements of a it issue-specific promoter from rice , P ( D54O ) , and tested the application of original and modified P ( D54O ) and its cis-elements in the regulation of gene expression .
P ( D54O ) is a green it issue-specific promoter .
Five novel it issue-specific cis-elements ( LPSE1 , LPSE2 , LPSRE1 , LPSRE2 , PSE1 ) were characterized from P ( D54O ) .
LPSE1 activated gene expression in leaf and young panicle .
LPSRE2 suppressed gene expression in leaf , root , young panicle and stem , and PSE1 suppressed gene expression in young panicle and stem .
LPSRE1 and LPSE2 had dual roles in the regulation of it issue-specific gene expression ; both functioned as activators in leaf , but LPSRE1 acted as a repressor in stem and LPSE2 as a repressor in young panicle and root .
Transgenic rice plants carrying cry1Ac encoding Bacillus thuringiensis endotoxin , regulated by P ( D54O ) , were resistant to leaf-folders , with no Cry1Ac protein found in endosperm or embryo .
A reporter gene regulated by a series of truncated P ( D54O ) showed various it issue-specific expression patterns .
Different fragments of P ( D54O ) fused with the constitutive cauliflower mosaic virus 35S promoter suppressed 35S-regulated gene expression in various it issues .
P ( D54O ) , truncated P ( D54O ) and the it issue-specific cis-elements provide useful tools for the regulation of it issue-specific gene expression in rice breeding programmes .
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[ Sen. 3, subscore: 2.00 ]: In this study , we identified the cis-acting elements of a it issue-specific promoter from rice , P ( D54O ) , and tested the application of original and modified P ( D54O ) and its cis-elements in the regulation of gene expression .
[ Sen. 12, subscore: 2.00 ]: P ( D54O ) , truncated P ( D54O ) and the it issue-specific cis-elements provide useful tools for the regulation of it issue-specific gene expression in rice breeding programmes .
[ Sen. 4, subscore: 1.00 ]: P ( D54O ) is a green it issue-specific promoter .
[ Sen. 5, subscore: 1.00 ]: Five novel it issue-specific cis-elements ( LPSE1 , LPSE2 , LPSRE1 , LPSRE2 , PSE1 ) were characterized from P ( D54O ) .
[ Sen. 9, subscore: 1.00 ]: Transgenic rice plants carrying cry1Ac encoding Bacillus thuringiensis endotoxin , regulated by P ( D54O ) , were resistant to leaf-folders , with no Cry1Ac protein found in endosperm or embryo .
[ Sen. 10, subscore: 1.00 ]: A reporter gene regulated by a series of truncated P ( D54O ) showed various it issue-specific expression patterns .
[ Sen. 11, subscore: 1.00 ]: Different fragments of P ( D54O ) fused with the constitutive cauliflower mosaic virus 35S promoter suppressed 35S-regulated gene expression in various it issues .
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Score: 7.00 |
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| Title: Categories and inheritance of resistance to Nilaparvata lugens ( Hemiptera : Delphacidae ) in mutants of indica rice IR64 .
| | Author: Sangha JS Chen YH Palchamy K Jahn GC Maheswaran M Adalla CB Leung H | | Journal: J Econ Entomol Citation: V : 101 P : 575-83 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18459427 Accession (PMID): 18459427 | | Abstract: Varietal mutants can be useful for developing durable resistance , understanding categories of resistance , and identifying candidate genes involved in defense responses .
We used mutants of rice IR64 to isolate new sources of resistance to the planthopper Nilaparvata lugens ( Stal ) ( Hemiptera : Delphacidae ) .
We compared two mutants that showed a gain and loss of resistance to N lugens , to determine the categories of resistance to this pest Under choice tests , female planthoppers avoided settling and laid fewer eggs on the resistant mutant D518 than on the susceptible mutant D1131 , susceptible check TN1 , and wild-type IR64 , indicating that antixenosis was the resistance category .
Similarly , under no-choice conditions , planthoppers laid 29% fewer eggs in D518 than in IR64 , but they oviposited more in D1131 and TN1 .
Honeydew excretion was greater on D1131 seedlings but slightly lower on D518 than on IR64 .
Nymphal survival and adult female weight did not differ among rice cultivars .
D518 showed higher tolerance of N lugens infestations than IR64 .
Genetic analysis of the F1 , F2 , and F3 populations derived from D518 x IR64 revealed that resistance in D518 is dominant and controlled by a single gene .
Despite the variation in resistance to N lugens , both mutants and IR64 performed similarly in the field .
The mutant D518 is a new source of durable resistance to N lugens , mainly due to enhanced antixenosis to female hoppers for settling and oviposition .
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[ Sen. 8, subscore: 2.00 ]: Genetic analysis of the F1 , F2 , and F3 populations derived from D518 x IR64 revealed that resistance in D518 is dominant and controlled by a single gene .
[ Sen. 3, subscore: 1.00 ]: We compared two mutants that showed a gain and loss of resistance to N lugens , to determine the categories of resistance to this pest Under choice tests , female planthoppers avoided settling and laid fewer eggs on the resistant mutant D518 than on the susceptible mutant D1131 , susceptible check TN1 , and wild-type IR64 , indicating that antixenosis was the resistance category .
[ Sen. 4, subscore: 1.00 ]: Similarly , under no-choice conditions , planthoppers laid 29% fewer eggs in D518 than in IR64 , but they oviposited more in D1131 and TN1 .
[ Sen. 5, subscore: 1.00 ]: Honeydew excretion was greater on D1131 seedlings but slightly lower on D518 than on IR64 .
[ Sen. 7, subscore: 1.00 ]: D518 showed higher tolerance of N lugens infestations than IR64 .
[ Sen. 10, subscore: 1.00 ]: The mutant D518 is a new source of durable resistance to N lugens , mainly due to enhanced antixenosis to female hoppers for settling and oviposition .
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Score: 6.00 |
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| Title: DWARF50 ( D50 ) , a rice ( Oryza sativa L ) gene encoding inositol polyphosphate 5-phosphatase , is required for proper development of intercalary meristem .
| | Author: Sato-Izawa K Nakaba S Tamura K Yamagishi Y Nakano Y Nishikubo N Kawai S Kajita S Ashikari M Funada R Katayama Y Kitano H | | Journal: Plant Cell Environ Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22574770 Accession (PMID): 22574770 | | Abstract: Rice internodes are vital for supporting high-yield panicles , which are controlled by various factors such as cell division , cell elongation and cell wall biosynthesis .
Therefore , formation and regulation of the internode cell-producing intercalary meristem ( IM ) are important for determining the shape of internodes .
To understand the regulation of internode development , we analysed a rice dwarf mutant , dwarf 50 ( d50 ) .
Previously , we reported that parenchyma cells in the elongated internodes of d50 ectopically deposit cell wall phenolics .
In this study , we revealed that D50 encodes putative inositol polyphosphate 5-phosphatase ( 5PTase ) , which may be involved in phosphoinositide signalling required for many essential cellular functions , such as cytoskeleton organization , endocytosis and vesicular trafficking in eukaryotes .
Analysis of the rice genome revealed 20 putative 5PTases including D50 .
The d50 mutation induced abnormally oriented cell division , irregular deposition of cell wall pectins and thick actin bundles in the parenchyma cells of the IM , resulting in abnormally organized cell files of the internode parenchyma and dwarf phenotype .
Our results suggest that the putative 5PTase , encoded by D50 , is essential for IM formation , including the direction of cell division , deposition of cell wall pectins and control of actin organization .
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[ Sen. 3, subscore: 1.00 ]: To understand the regulation of internode development , we analysed a rice dwarf mutant , dwarf 50 ( d50 ) .
[ Sen. 4, subscore: 1.00 ]: Previously , we reported that parenchyma cells in the elongated internodes of d50 ectopically deposit cell wall phenolics .
[ Sen. 5, subscore: 1.00 ]: In this study , we revealed that D50 encodes putative inositol polyphosphate 5-phosphatase ( 5PTase ) , which may be involved in phosphoinositide signalling required for many essential cellular functions , such as cytoskeleton organization , endocytosis and vesicular trafficking in eukaryotes .
[ Sen. 6, subscore: 1.00 ]: Analysis of the rice genome revealed 20 putative 5PTases including D50 .
[ Sen. 7, subscore: 1.00 ]: The d50 mutation induced abnormally oriented cell division , irregular deposition of cell wall pectins and thick actin bundles in the parenchyma cells of the IM , resulting in abnormally organized cell files of the internode parenchyma and dwarf phenotype .
[ Sen. 8, subscore: 1.00 ]: Our results suggest that the putative 5PTase , encoded by D50 , is essential for IM formation , including the direction of cell division , deposition of cell wall pectins and control of actin organization .
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Score: 5.00 |
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| Title: Mutations in the rpoB and katG genes leading to drug resistance in Mycobacterium tuberculosis in Latvia .
| | Author: Tracevska T Jansone I Broka L Marga O Baumanis V | | Journal: J Clin . Microbiol . Citation: V : 40 ( 10 ) P : 3789-92 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12354882 Accession (PMID): 12354882 | | Abstract: To characterize the genetic basis of drug resistance in Mycobacterium tuberculosis in Latvia , mutations involved in rifampin ( rpoB gene ) and isoniazid ( katG gene ) resistance in DNA from 19 drug-susceptible and 51 multidrug-resistant M tuberculosis complex isolates were analyzed .
The most frequent rpoB gene mutations found by the Line Probe assay were the S531L ( 14 of 34 isolates ) , D516V ( 7 of 34 ) , H526D ( 4 of 34 ) , and D516Y plus P535S ( 4 of 34 ) mutations .
Direct sequencing of seven isolates with unclear results from Line Probe assay showed the presence of the L533P mutation and the Q510H plus H526Y ( 1 of 34 ) and D516V plus P535S ( 4 of 34 ) double mutations , neither of which has been described previously .
Single-strand conformation polymorphism analysis showed strand mobility differences between the rifampin-susceptible and -resistant samples for the D516V , H526D , and D516Y plus P535S mutations but not for the S531L mutation .
Nucleotide substitution at codon 315 ( AGC-->ACC ) of the katG gene was found in 48 of 51 multidrug-resistant samples by sequencing .
Furthermore , katG gene restriction fragment length polymorphism analysis with endonuclease AciI confirmed the nucleotide change in codon 315 . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: The most frequent rpoB gene mutations found by the Line Probe assay were the S531L ( 14 of 34 isolates ) , D516V ( 7 of 34 ) , H526D ( 4 of 34 ) , and D516Y plus P535S ( 4 of 34 ) mutations .
[ Sen. 4, subscore: 2.00 ]: Single-strand conformation polymorphism analysis showed strand mobility differences between the rifampin-susceptible and -resistant samples for the D516V , H526D , and D516Y plus P535S mutations but not for the S531L mutation .
[ Sen. 3, subscore: 1.00 ]: Direct sequencing of seven isolates with unclear results from Line Probe assay showed the presence of the L533P mutation and the Q510H plus H526Y ( 1 of 34 ) and D516V plus P535S ( 4 of 34 ) double mutations , neither of which has been described previously .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: The ectopic expression of phenylalanine ammonia lyase with ectopic accumulation of polysaccharide-linked hydroxycinnamoyl esters in internode parenchyma of rice mutant Fukei 71 .
| | Author: Mase K Sato K Nakano Y Nishikubo N Tsuboi Y Zhou J Kitano H Katayama Y | | Journal: Plant Cell Rep .
Citation: V : 24 ( 8 ) P : 487-93 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15838683 Accession (PMID): 15838683 | | Abstract: Both polysaccharide-linked hydroxycinnamoyl esters ( PHEs ) and lignin are biosynthesized via the phenylpropanoid pathway .
In the abnormal internode parenchyma of the rice ( Oryza sativa L ) mutant Fukei 71 , which has a defective recessive gene ( d50 ) , the biosynthesis of lignin and PHEs differs .
. The polysaccharide-linked ferulate and p-coumarate have been shown to accumulate to high levels in the irregularly shaped and collapsed internode parenchyma cells of Fukei 71 without an accompanying overaccumulation of lignin as a result of the defective d50 gene .
In the present study we demonstrated that in this abnormal parenchyma it issue of Fukei 71 the expression of phenylalanine ammonia lyase ( PAL ) and glutamine synthetase ( GS ) were ectopically induced with the ectopic accumulation of PHEs , suggesting that the d50 gene may play a role as a controlling element in the biosynthesis of PHEs during cell-wall formation in the grasses . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In the abnormal internode parenchyma of the rice ( Oryza sativa L ) mutant Fukei 71 , which has a defective recessive gene ( d50 ) , the biosynthesis of lignin and PHEs differs .
[ Sen. 3, subscore: 1.00 ]: . The polysaccharide-linked ferulate and p-coumarate have been shown to accumulate to high levels in the irregularly shaped and collapsed internode parenchyma cells of Fukei 71 without an accompanying overaccumulation of lignin as a result of the defective d50 gene .
[ Sen. 4, subscore: 1.00 ]: In the present study we demonstrated that in this abnormal parenchyma it issue of Fukei 71 the expression of phenylalanine ammonia lyase ( PAL ) and glutamine synthetase ( GS ) were ectopically induced with the ectopic accumulation of PHEs , suggesting that the d50 gene may play a role as a controlling element in the biosynthesis of PHEs during cell-wall formation in the grasses .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
