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2 matches found in 2 documents. Search time: 0.077 seconds.
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Score: 1.00
Title: Cloning , purification , crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein .
Author: Abd Aziz AG Ruzheinikov SN Sedelnikova SE Mohamed R Nathan S Baker PJ Rice DW
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Citation: V : 68 P : 347-50 Year: 2012 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub22442241 Accession (PMID): 22442241
Abstract: The gene encoding the L1 ribosomal protein from Burkholderia pseudomallei strain D286 has been cloned into the pETBLUE-1 vector system , overexpressed in Escherichia coli and purified . Crystals of the native protein were grown by the hanging-drop vapour-diffusion technique using PEG 3350 as a precipitant and diffracted to beyond 1 . 65 A resolution . The crystals belonged to space group P2 ( 1 ) 2 ( 1 ) 2 , with unit-cell parameters a = 53 . 6 , b = 127 . 1 , c = 31 . 8 A and with a single molecule in the asymmetric unit .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The gene encoding the L1 ribosomal protein from Burkholderia pseudomallei strain D286 has been cloned into the pETBLUE-1 vector system , overexpressed in Escherichia coli and purified .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator , BPSL0128 , from Burkholderia pseudomallei .
Author: Abd Aziz AG Sedelnikova SE Ruzheinikov SN Thorpe S Mohamed R Nathan S Rafferty JB Baker PJ Rice DW
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Citation: V : 68 P : 917-22 Year: 2012 Type: In-Data-Review
Literature: oryza Field: abstract Doc ID: pub22869122 Accession (PMID): 22869122
Abstract: bpsl0128 , a gene encoding a putative response regulator from Burkholderia pseudomallei strain D286 , has been cloned into a pETBLUE-1 vector system , overexpressed in Escherichia coli and purified . The full-length protein is degraded during purification to leave a fragment corresponding to the putative receiver domain , and crystals of this protein that diffracted to beyond 1 . 75 A resolution have been grown by the hanging-drop vapour-diffusion technique using PEG 6000 as the precipitant . The crystals belonged to one of the enantiomorphic pair of space groups P3 ( 1 ) 21 and P3 ( 2 ) 21 , with unit-cell parameters a = b = 65 . 69 , c = 105 . 01 A and either one or two molecules in the asymmetric unit .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: bpsl0128 , a gene encoding a putative response regulator from Burkholderia pseudomallei strain D286 , has been cloned into a pETBLUE-1 vector system , overexpressed in Escherichia coli and purified .
Supplemental links/files: reference in endnote online text related articles pubmed citation
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