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Score: 1.00
Title: Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses .
Author: Peres A Churchman ML Hariharan S Himanen K Verkest A Vandepoele K Magyar Z Hatzfeld Y Van Der Schueren E Beemster GT Frankard V Larkin JC Inze D De Veylder L
Journal: J Biol Chem Citation: V : 282 P : 25588-96 Year: 2007 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub17599908 Accession (PMID): 17599908
Abstract: The EL2 gene of rice ( Oryza sativa ) , previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function , was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM ) gene of Arabidopsis thaliana . Iterative two-hybrid screens , in vitro pull-down assays , and fluorescence resonance energy transfer analyses showed that Orysa ; EL2 binds the cyclin-dependent kinase ( CDK ) CDKA1 ; 1 and D-type cyclins . No interaction was observed with the plant-specific B-type CDKs . The amino acid motif ELERFL was identified to be essential for cyclin , but not for CDK binding . Orysa ; EL2 impaired the ability of Orysa ; CYCD5 ; 3 to complement a budding yeast ( Saccharomyces cerevisiae ) triple CLN mutant , whereas recombinant protein inhibited CDK activity in vitro . Moreover , Orysa ; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis , unequivocally demonstrating that Orysa ; EL2 operates as a cell cycle inhibitor . Orysa ; EL2 mRNA levels were induced by cold , drought , and propionic acid . Our data suggest that Orysa ; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses .
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[ Sen. 5, subscore: 1.00 ]: Orysa ; EL2 impaired the ability of Orysa ; CYCD5 ; 3 to complement a budding yeast ( Saccharomyces cerevisiae ) triple CLN mutant , whereas recombinant protein inhibited CDK activity in vitro .
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