| 6 matches found in 3 documents. Search time: 0.63 seconds. |
|---|
|
Score: 4.00 |
|---|
| Title: The expression of Orysa ; CycB1 ; 1 is essential for endosperm formation and causes embryo enlargement in rice .
| | Author: Guo J Wang F Song J Sun W Zhang XS | | Journal: Planta Citation: V : 231 P : 293-303 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19921249 Accession (PMID): 19921249 | | Abstract: The cell cycle is an important process during seed development in plants and its progression is driven by a number of core regulators such as the cyclins .
Currently , however , little is known regarding the role of the cyclins in embryo and endosperm development in cereals .
In our current study , we show that the knockdown of Orysa ; CycB1 ; 1 in rice results in the production of abnormal seeds , which at maturity contain only an enlarged embryo .
It was further found that a delayed and abnormal cellularization occurred in the endosperm in these knockdown seeds which eventually became abortive .
Moreover , the observed development of the enlarged embryo was also morphologically abnormal and found to be caused by an enlarged cell size rather than an increased cell number .
Expression analysis showed that Orysa ; CycB1 ; 1 transcripts were localized in the endosperm and embryo .
Genome-wide transcriptional profiling further indicated that a large number of genes are responsible for the phenotype of the enlarged embryo .
The results of the knockdown of Orysa ; CycB1 ; 1 via an endosperm or an embryo-specific promoter also suggest that the enlarged embryo may be correlated to the abortive endosperm .
Our results suggest that Orysa ; CycB1 ; 1 expression is critical for endosperm formation via the regulation of mitotic division , and that the endosperm plays an important role in maintenance of embryo development in rice .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: In our current study , we show that the knockdown of Orysa ; CycB1 ; 1 in rice results in the production of abnormal seeds , which at maturity contain only an enlarged embryo .
[ Sen. 6, subscore: 1.00 ]: Expression analysis showed that Orysa ; CycB1 ; 1 transcripts were localized in the endosperm and embryo .
[ Sen. 8, subscore: 1.00 ]: The results of the knockdown of Orysa ; CycB1 ; 1 via an endosperm or an embryo-specific promoter also suggest that the enlarged embryo may be correlated to the abortive endosperm .
[ Sen. 9, subscore: 1.00 ]: Our results suggest that Orysa ; CycB1 ; 1 expression is critical for endosperm formation via the regulation of mitotic division , and that the endosperm plays an important role in maintenance of embryo development in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Interacting proteins and differences in nuclear transport reveal specific functions for the NAP1 family proteins in plants .
| | Author: Dong A Liu Z Zhu Y Yu F Li Z Cao K Shen WH .
| | Journal: Plant Physiol .
Citation: V : 138 ( 3 ) P : 1446-56 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15980199 Accession (PMID): 15980199 | | Abstract: Nucleosome assembly protein 1 ( NAP1 ) is conserved from yeast to human and facilitates the in vitro assembly of nucleosomes as a histone chaperone .
Inconsistent with their proposed function in the nucleus , however , many NAP1 proteins had been reported to localize in the cytoplasm .
We investigated the subcellular localization of tobacco ( Nicotiana tabacum ) and rice ( Oryza sativa ) NAP1 family proteins first by identification of interacting partners and by direct examination of the localization of green fluorescent protein-tagged proteins .
Through treatment of tobacco cells with leptomycin B and mutagenesis of nuclear export signal , we demonstrated that Nicta ; NAP1 ; 1 and Orysa ; NAP1 ; 1 shuttle between the cytoplasm and the nucleus .
Together with the demonstration that tobacco NAP1 proteins bind histone H2A and H2B , our results support the current model and provide additional evidence that function of NAP1 as histone chaperones appears to be conserved in plants .
In addition , we show that tobacco NAP1 proteins interact with tubulin and the mitotic cyclin Nicta ; CYCB1 ; 1 , suggesting a role for NAP1 in microtubule dynamics .
Interestingly , in spite of their high homology with the above NAP1 proteins , the other three tobacco proteins and Orysa ; NAP1 ; 2 did not show nucleocytoplasmic shuttling and were localized only in the cytoplasm .
Moreover , Orysa ; NAP1 ; 3 that lacks a typical nuclear localization signal sequence was localized in both the cytoplasm and the nucleus .
Finally , we show that only Orysa ; NAP1 ; 3 could be phosphorylated by casein kinase 2alpha in vitro .
However , this phosphorylation was not responsible for nuclear import of Orysa ; NAP1 ; 3 as being demonstrated through mutagenesis studies .
Together , our results provide an important step toward elucidating the molecular mechanism of function of the NAP1 family proteins in plants . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: In addition , we show that tobacco NAP1 proteins interact with tubulin and the mitotic cyclin Nicta ; CYCB1 ; 1 , suggesting a role for NAP1 in microtubule dynamics .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: The expression of Orysa ; CycB1 ; 1 is essential for endosperm formation and causes embryo enlargement in rice .
| | Author: Guo J Wang F Song J Sun W Zhang XS | | Journal: Planta Citation: V : 231 P : 293-303 Year: 2010 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub19921249 Accession (PMID): 19921249 | | Abstract: The cell cycle is an important process during seed development in plants and its progression is driven by a number of core regulators such as the cyclins .
Currently , however , little is known regarding the role of the cyclins in embryo and endosperm development in cereals .
In our current study , we show that the knockdown of Orysa ; CycB1 ; 1 in rice results in the production of abnormal seeds , which at maturity contain only an enlarged embryo .
It was further found that a delayed and abnormal cellularization occurred in the endosperm in these knockdown seeds which eventually became abortive .
Moreover , the observed development of the enlarged embryo was also morphologically abnormal and found to be caused by an enlarged cell size rather than an increased cell number .
Expression analysis showed that Orysa ; CycB1 ; 1 transcripts were localized in the endosperm and embryo .
Genome-wide transcriptional profiling further indicated that a large number of genes are responsible for the phenotype of the enlarged embryo .
The results of the knockdown of Orysa ; CycB1 ; 1 via an endosperm or an embryo-specific promoter also suggest that the enlarged embryo may be correlated to the abortive endosperm .
Our results suggest that Orysa ; CycB1 ; 1 expression is critical for endosperm formation via the regulation of mitotic division , and that the endosperm plays an important role in maintenance of embryo development in rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The expression of Orysa ; CycB1 ; 1 is essential for endosperm formation and causes embryo enlargement in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
