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Title: Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media .
Author: Wu F Yan H Ma X Jia J Zhang G Guo X Gui Z
Journal: World J Microbiol Biotechnol Citation: V : 28 P : 2029-38 Year: 2012 Type: In-Data-Review
Literature: oryza Field: abstract Doc ID: pub22806024 Accession (PMID): 22806024
Abstract: Two acidic polysaccharide fractions , CM-jd-CPS2 and CM-jd ( Y ) -CPS2 , were isolated from the fruiting bodies of cultured Cordyceps militaris grown on solid rice medium and silkworm pupa , respectively , by hot-water extraction , ethanol precipitation and fractionation using ion-exchange column ( DEAE-cellulose-52 ) and gel-filtration column ( Sephadex G-100 ) chromatography . Their structural characterizations were performed by gas chromatography and fourier-transform infrared spectroscopy . Some differences existed between their structures , which indicated that culture media could influence the structure of polysaccharides of C militaris . The antioxidant activities of CM-jd-CPS2 and CM-jd ( Y ) -CPS2 were evaluated by various methods in vitro . They had strong 2 , 2-diphenyl-1-picrylhydrazyl radical-scavenging activity and ferrous ion-chelating capacity , but moderate reducing power . The antioxidant activities of CM-jd ( Y ) -CPS2 were slightly higher than those of CM-jd-CPS2 . These two acidic fractions were evaluated for proliferation of mouse splenocyte activity in vitro . They both possessed does-dependent mitogenic effects on mouse splenocytes , and could synergistically promote murine T and B-lymphocytes induced by Con A and LPS . CM-jd ( Y ) -CPS2 exhibited stronger stimulatory activities upon immunomodulation than CM-jd-CPS2 . These results are beneficial for the interpretation of the connection between polysaccharide structures and their biological activities .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Two acidic polysaccharide fractions , CM-jd-CPS2 and CM-jd ( Y ) -CPS2 , were isolated from the fruiting bodies of cultured Cordyceps militaris grown on solid rice medium and silkworm pupa , respectively , by hot-water extraction , ethanol precipitation and fractionation using ion-exchange column ( DEAE-cellulose-52 ) and gel-filtration column ( Sephadex G-100 ) chromatography .
[ Sen. 4, subscore: 1.00 ]: The antioxidant activities of CM-jd-CPS2 and CM-jd ( Y ) -CPS2 were evaluated by various methods in vitro .
[ Sen. 6, subscore: 1.00 ]: The antioxidant activities of CM-jd ( Y ) -CPS2 were slightly higher than those of CM-jd-CPS2 .
[ Sen. 9, subscore: 1.00 ]: CM-jd ( Y ) -CPS2 exhibited stronger stimulatory activities upon immunomodulation than CM-jd-CPS2 .
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