Score: 2.00 |
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| Title: Enhanced sheath blight resistance in transgenic rice expressing an endochitinase gene from Trichoderma virens .
| | Author: Shah JM Raghupathy V Veluthambi K | | Journal: Biotechnol Lett Citation: V : 31 P : 239-44 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18923909 Accession (PMID): 18923909 | | Abstract: The 42-kDa endochitinase ( cht42 ) gene from the mycoparasitic fungus , Trichoderma virens , driven by CaMV 35S promoter , was introduced into rice by Agrobacterium-mediated transformation .
Eight transgenic plants harboring single copies of complete T-DNA were identified by Southern blot analysis .
Homozygous transgenic plants were identified for five lines in the T ( 1 ) generation by Southern blot analysis .
Homozygous T ( 2 ) plants constitutively accumulated high levels of the cht42 transcript , showed 2 . 4 to 4 . 6-fold higher chitinase activity in total leaf extract and 1 . 6 to 1 . 8-fold higher chitinase activity in the extracellular fluid .
Infection assays performed on the homozygous T ( 2 ) plants with Rhizoctonia solani showed up to 62% reduction in the sheath blight disease index | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The 42-kDa endochitinase ( cht42 ) gene from the mycoparasitic fungus , Trichoderma virens , driven by CaMV 35S promoter , was introduced into rice by Agrobacterium-mediated transformation .
[ Sen. 4, subscore: 1.00 ]: Homozygous T ( 2 ) plants constitutively accumulated high levels of the cht42 transcript , showed 2 . 4 to 4 . 6-fold higher chitinase activity in total leaf extract and 1 . 6 to 1 . 8-fold higher chitinase activity in the extracellular fluid .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Distribution , structure , organ-specific expression , and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice ( Oryza sativa L ) .
| | Author: Nakazaki T Tsukiyama T Okumoto Y Kageyama D Naito K Inouye K Tanisaka T | | Journal: Genome Citation: V : 49 ( 6 ) P : 619-30 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16936841 Accession (PMID): 16936841 | | Abstract: Rice ( Oryza sativa L ) pathogenesis-related ( PR ) -3 chitinases , like other PR proteins , are each coded by one of the genes of a multigene family in the plant genome .
We assembled the database information about rice PR-3 chitinase sequences .
A total of 12 PR-3 chitinase loci ( Cht1 to Cht12 ) were found deployed in the rice genome .
Some of the loci were occupied by 2 or more alleles .
For all the loci expect Cht4 , Cht5 , Cht6 , and Cht11 , the amino acid sequence was polymorphic between japonica and indica varieties of rice , but glutamic acid acting as a catalytic residue was completely conserved in all the loci expect Cht7 .
All the genes except Cht7 , which was not tested in this study , were transcripted in some organs ( leaf , sheath , root , and meristem ) of rice plants .
These results suggest that chitinase proteins encoded by the genes at these loci have important biological effects , at least antifungal activities , on rice plants .
We also proposed a new classification of rice PR-3 chitinases based on their domain structures .
This classification was consistent with the results of phylogenetic analysis of rice chitinases . | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: For all the loci expect Cht4 , Cht5 , Cht6 , and Cht11 , the amino acid sequence was polymorphic between japonica and indica varieties of rice , but glutamic acid acting as a catalytic residue was completely conserved in all the loci expect Cht7 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
