| 14 matches found in 5 documents. Search time: 0.209 seconds. |
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Score: 8.00 |
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| Title: Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase .
| | Author: Lee J Das A Yamaguchi M Hashimoto J Tsutsumi N Uchimiya H Umeda M | | Journal: Plant J Citation: V : 34 ( 4 ) P : 417-25 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12753582 Accession (PMID): 12753582 | | Abstract: Cyclin-dependent kinases ( CDKs ) are involved in the control of cell cycle progression .
Plant A-type CDKs are functional homologs of yeast Cdc2/Cdc28 and are expressed throughout the cell cycle .
In contrast , B-type CDK ( CDKB ) is a family of mitotic CDKs expressed during the S/M phase , and its precise function remains unknown .
Here , we identified two B2-type cyclins , CycB2 ; 1 and CycB2 ; 2 , as a specific partner of rice CDKB2 ; 1 .
The CDKB2 ; 1-CycB2 complexes produced in insect cells showed a significant level of kinase activity in vitro , suggesting that CycB2 binds to and activates CDKB2 .
We then expressed green fluorescent protein ( GFP ) -fused CDKB2 ; 1 and CycB2 ; 2 in tobacco BY2 cells to investigate their subcellular localization during mitosis .
Surprisingly , the fluorescence signal of CDKB2 ; 1-GFP was tightly associated with chromosome alignment as well as with spindle structure during the metaphase .
During the telophase , the signal was localized to the spindle midzone and the separating sister chromosomes , and then to the phragmoplast On the other hand , the CycB2 ; 2-GFP fluorescence signal was detected in nuclei during the interphase and prophase , moved to the metaphase chromosomes , and then disappeared completely after the cells passed through the metaphase .
Co-localization of CDKB2 ; 1-GFP and CycB2 ; 2-GFP on chromosomes aligned at the center of the metaphase cells suggests that the CDKB2-CycB2 complex may function in retaining chromosomes at the metaphase plate .
Overexpression of CycB2 ; 2 in rice plants resulted in acceleration of root growth without any increase in cell size , indicating that CycB2 ; 2 promoted cell division probably through association with CDKB2 in the root meristem . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: The CDKB2 ; 1-CycB2 complexes produced in insect cells showed a significant level of kinase activity in vitro , suggesting that CycB2 binds to and activates CDKB2 .
[ Sen. 9, subscore: 2.00 ]: Co-localization of CDKB2 ; 1-GFP and CycB2 ; 2-GFP on chromosomes aligned at the center of the metaphase cells suggests that the CDKB2-CycB2 complex may function in retaining chromosomes at the metaphase plate .
[ Sen. 4, subscore: 1.00 ]: Here , we identified two B2-type cyclins , CycB2 ; 1 and CycB2 ; 2 , as a specific partner of rice CDKB2 ; 1 .
[ Sen. 6, subscore: 1.00 ]: We then expressed green fluorescent protein ( GFP ) -fused CDKB2 ; 1 and CycB2 ; 2 in tobacco BY2 cells to investigate their subcellular localization during mitosis .
[ Sen. 7, subscore: 1.00 ]: Surprisingly , the fluorescence signal of CDKB2 ; 1-GFP was tightly associated with chromosome alignment as well as with spindle structure during the metaphase .
[ Sen. 10, subscore: 1.00 ]: Overexpression of CycB2 ; 2 in rice plants resulted in acceleration of root growth without any increase in cell size , indicating that CycB2 ; 2 promoted cell division probably through association with CDKB2 in the root meristem .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: CDKB2 is involved in mitosis and DNA damage response in rice .
| | Author: Endo M Nakayama S Umeda-Hara C Ohtsuki N Saika H Umeda M Toki S | | Journal: Plant J Citation: V : 69 P : 967-77 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22092531 Accession (PMID): 22092531 | | Abstract: DNA damage checkpoints delay mitotic cell-cycle progression in response to DNA stress , stalling the cell cycle to allow time for repair .
CDKB is a plant-specific cyclin-dependent kinase ( CDK ) that is required for the G ( 2 ) /M transition of the cell cycle .
In Arabidopsis , DNA damage leads the degradation of CDKB2 , and the subsequent G ( 2 ) arrest gives cells time to repair damaged DNA .
G ( 2 ) arrest also triggers transition from the mitotic cycle to endoreduplication , leading to the presence of polyploid cells in many it issues .
In contrast , in rice ( Oryza sativa ) , polyploid cells are found only in the endosperm .
It was unclear whether endoreduplication contributes to alleviating DNA damage in rice ( Oryza sativa ) .
Here , we show that DNA damage neither down-regulates Orysa ; CDKB2 ; 1 nor induces endoreduplication in rice .
Furthermore , we found increased levels of Orysa ; CDKB2 ; 1 protein upon DNA damage .
These results suggest that CDKB2 functions differently in Arabidopsis and rice in response to DNA damage .
Arabidopsis may adopt endoreduplication as a survival strategy under genotoxic stress conditions , but rice may enhance DNA repair capacity upon genotoxic stress .
In addition , polyploid cells due to endomitosis were present in CDKB2 ; 1 knockdown rice , suggesting an important role for Orysa ; CDKB2 ; 1 during mitosis .
| Matching Sentences:
[ Sen. 11, subscore: 2.00 ]: In addition , polyploid cells due to endomitosis were present in CDKB2 ; 1 knockdown rice , suggesting an important role for Orysa ; CDKB2 ; 1 during mitosis .
[ Sen. 3, subscore: 1.00 ]: In Arabidopsis , DNA damage leads the degradation of CDKB2 , and the subsequent G ( 2 ) arrest gives cells time to repair damaged DNA .
[ Sen. 7, subscore: 1.00 ]: Here , we show that DNA damage neither down-regulates Orysa ; CDKB2 ; 1 nor induces endoreduplication in rice .
[ Sen. 8, subscore: 1.00 ]: Furthermore , we found increased levels of Orysa ; CDKB2 ; 1 protein upon DNA damage .
[ Sen. 9, subscore: 1.00 ]: These results suggest that CDKB2 functions differently in Arabidopsis and rice in response to DNA damage .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Increased expression of the rice C-type cyclin-dependent protein kinase gene , Orysa ; CDKC ; 1 , in response to salt stress .
| | Author: Huang YW Tsay WS Chen CC Lin CW Huang HJ | | Journal: Plant Physiol Biochem Citation: V : 46 P : 71-81 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18054244 Accession (PMID): 18054244 | | Abstract: Cyclin-dependent protein kinases ( CDKs ) form a conserved superfamily of eukaryotic serine/threonine protein kinases which require binding to a regulatory cyclin for activity .
CDKs are organized in several gene families and are involved in different aspects of cell biology , such as gene transcription , cell proliferation and differentiation .
In this study , we present the characteristics of the rice C-type CDK gene , Orysa ; CDKC ; 1 .
The gene was expressed in all organs of the plant and relatively high amounts of its transcript were detected in mature leaves and panicles .
This indicated a putative role during later stages of leaf and panicle development .
Using yeast two-hybrid analysis , we identified Orysa ; CYCT as an interactor with Orysa ; CDKC ; 1 .
The expression profiles of the new Orysa ; CDKC ; 1 in response to various factors from rice cells were compared with those of previously described Orysa ; CDKA ; 1 , and Orysa ; CDKB2 ; 1 .
The Orysa ; CDKC ; 1 transcript was induced by salt stress and ABA treatments , but was unaffected by mannitol .
In addition , the MBP kinase activity associated with Orysa ; CDKC ; 1 immunocomplexes was induced under salt stress .
We propose that Orysa ; CDKC ; 1 may be involved in developmental programs , as well as in the salt and ABA-signaling pathway .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The expression profiles of the new Orysa ; CDKC ; 1 in response to various factors from rice cells were compared with those of previously described Orysa ; CDKA ; 1 , and Orysa ; CDKB2 ; 1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The syncytium-specific expression of the Orysa ; KRP3 CDK inhibitor : implication of its involvement in the cell cycle control in the rice ( Oryza sativa L ) syncytial endosperm .
| | Author: Mizutani M Naganuma T Tsutsumi K Saitoh Y | | Journal: J Exp Bot Citation: V : 61 P : 791-8 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19933315 Accession (PMID): 19933315 | | Abstract: During rice ( Oryza sativa L ) seed development , the primary endosperm nucleus undergoes a series of divisions without cytokinesis , producing a multinucleate cell , known as a syncytium .
After several rounds of rapid nuclear proliferation , the syncytium ceases to undergo mitosis ; thereafter , the syncytium is partitioned into individual cells by a specific type of cytokinesis called cellularization .
The transition between syncytium and cellularization is important in determining the final seed size and is a model for studying the cell cycle and cytokinesis .
The involvement of cyclin-dependent kinase ( CDK ) inhibitors ( CKIs ) in cell cycle control was investigated here during the transition between syncytium and cellularization .
It was found that one of the rice CKIs , Orysa ; KRP3 , is strongly expressed in the caryopsis at 2 d after flowering ( DAF ) , and its expression is significantly reduced at 3 DAF .
The other CKI transcripts did not show such a shift at 2 DAF .
In situ hybridization analysis revealed that Orysa ; KRP3 is expressed in multinucleate syncytial endosperm at 2 DAF , but not in cellularized endosperm at 3 DAF .
Two-hybrid assays showed that Orysa ; KRP3 binds Orysa ; CDKA ; 1 , Orysa ; CDKA ; 2 , Orysa ; CycA1 ; 1 , and Orysa ; CycD2 ; 2 .
By contrast , Orysa ; CDKB2 ; 1 and Orysa ; CycB2 ; 2 do not show binding to Orysa ; KRP3 .
Orysa ; KRP3 was able to rescue yeast premature cell division due to the dominant positive expression of mutant rice CDKA ; 1 indicating that Orysa ; KRP3 inhibited rice CDK .
These data suggest that Orysa ; KRP3 is involved in cell cycle control of syncytial endosperm .
| Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: By contrast , Orysa ; CDKB2 ; 1 and Orysa ; CycB2 ; 2 do not show binding to Orysa ; KRP3 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: CDKB2 is involved in mitosis and DNA damage response in rice .
| | Author: Endo M Nakayama S Umeda-Hara C Ohtsuki N Saika H Umeda M Toki S | | Journal: Plant J Citation: V : 69 P : 967-77 Year: 2012 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub22092531 Accession (PMID): 22092531 | | Abstract: DNA damage checkpoints delay mitotic cell-cycle progression in response to DNA stress , stalling the cell cycle to allow time for repair .
CDKB is a plant-specific cyclin-dependent kinase ( CDK ) that is required for the G ( 2 ) /M transition of the cell cycle .
In Arabidopsis , DNA damage leads the degradation of CDKB2 , and the subsequent G ( 2 ) arrest gives cells time to repair damaged DNA .
G ( 2 ) arrest also triggers transition from the mitotic cycle to endoreduplication , leading to the presence of polyploid cells in many it issues .
In contrast , in rice ( Oryza sativa ) , polyploid cells are found only in the endosperm .
It was unclear whether endoreduplication contributes to alleviating DNA damage in rice ( Oryza sativa ) .
Here , we show that DNA damage neither down-regulates Orysa ; CDKB2 ; 1 nor induces endoreduplication in rice .
Furthermore , we found increased levels of Orysa ; CDKB2 ; 1 protein upon DNA damage .
These results suggest that CDKB2 functions differently in Arabidopsis and rice in response to DNA damage .
Arabidopsis may adopt endoreduplication as a survival strategy under genotoxic stress conditions , but rice may enhance DNA repair capacity upon genotoxic stress .
In addition , polyploid cells due to endomitosis were present in CDKB2 ; 1 knockdown rice , suggesting an important role for Orysa ; CDKB2 ; 1 during mitosis .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: CDKB2 is involved in mitosis and DNA damage response in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
