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Score: 1.00
Title: A characterization of a MutM/Fpg ortholog in sugarcane--A monocot plant .
Author: Scortecci KC Lima AF Carvalho FM Silva UB Agnez-Lima LF Batistuzzo de Medeiros SR
Journal: Biochem Biophys Res Commun Citation: V : 361 P : 1054-60 Year: 2007 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub17686457 Accession (PMID): 17686457
Abstract: Plant genomic projects , such as Arabidopsis thaliana , rice , and maize , have provided excellent tools for comparative genome analysis on Base Excision DNA Repair ( BER ) . A data-mining study associated with the SUCEST Genome project identified two EST clusters that shared homology to the bacteria MutM/Fpg protein . Comparative analyses presented here show a duplication of the MutM/Fpg gene in sugarcane , wheat and rice . The complementation assays show that both cDNAs from sugarcane are able to complement the Fpg and MutY-glycosylase deficiency in a double mutant Escherichia coli strain ( CC104mutMmutY ) , reducing the spontaneous mutation frequency by 10-fold . The expression analyses by semi-quantitative RT-PCR show that these two mRNAs have different expression levels .
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[ Sen. 4, subscore: 1.00 ]: The complementation assays show that both cDNAs from sugarcane are able to complement the Fpg and MutY-glycosylase deficiency in a double mutant Escherichia coli strain ( CC104mutMmutY ) , reducing the spontaneous mutation frequency by 10-fold .
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