| 28 matches found in 22 documents. Search time: 0.389 seconds. |
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| Title: Screening for IgE mediated allergy among people working in the Marseilles harbour .
| | Author: Panzani RC Falagiani P Riva G Mercier P Delord . | | Journal: Citation: V : 21 ( 1 ) P : 25-9 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8328353 Accession (PMID): 8328353 | | Abstract: Screening for IgE mediated allergy by RASTs to professional ( castor bean , green coffee , peanut , soy protein , wheat , rice ) , and non professional ( pollens , mites , cat , Alternaria tenuis ) air borne antigens among 36 people working in the Marseilles harbour has showed rather unexpected findings : only one case of IgE positivity to Dermatophagoides pteronyssinus ( class I ) and one case of IgE positivity to castor bean seed ( Ricinus communis ) ( class IV ) .
IgG4 specific antibodies against castor bean and green coffee were also measured by an ELISA technique , with eleven cases of positivity to castor bean and only one case to green coffee being recorded .
Several explanations can be put forward for the low incidence of IgE responses to the commonest airborne antigens and to the professional antigens ( castor bean being the only offender ) , and for the rather high incidence of specific IgG4 antibodies to castor bean .
Most likely , the low incidence of latent atopy is the result of a natural selection among the workers who gave up their job if experiencing of discomfort .
As far as the elevated IgG4 antibody levels to castor bean are concerned , these are probably natural blocking antibodies .
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[ Sen. 1, subscore: 2.00 ]: Screening for IgE mediated allergy by RASTs to professional ( castor bean , green coffee , peanut , soy protein , wheat , rice ) , and non professional ( pollens , mites , cat , Alternaria tenuis ) air borne antigens among 36 people working in the Marseilles harbour has showed rather unexpected findings : only one case of IgE positivity to Dermatophagoides pteronyssinus ( class I ) and one case of IgE positivity to castor bean seed ( Ricinus communis ) ( class IV ) .
[ Sen. 2, subscore: 2.00 ]: IgG4 specific antibodies against castor bean and green coffee were also measured by an ELISA technique , with eleven cases of positivity to castor bean and only one case to green coffee being recorded .
[ Sen. 3, subscore: 2.00 ]: Several explanations can be put forward for the low incidence of IgE responses to the commonest airborne antigens and to the professional antigens ( castor bean being the only offender ) , and for the rather high incidence of specific IgG4 antibodies to castor bean .
[ Sen. 5, subscore: 1.00 ]: As far as the elevated IgG4 antibody levels to castor bean are concerned , these are probably natural blocking antibodies .
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Score: 2.00 |
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| Title: Antiquity and function of CASTOR and POLLUX , the twin ion channel-encoding genes key to the evolution of root symbioses in plants .
| | Author: Chen C Fan C Gao M Zhu H | | Journal: Plant Physiol Citation: V : 149 P : 306-17 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18978069 Accession (PMID): 18978069 | | Abstract: Root symbioses with arbuscular mycorrhizal fungi and rhizobial bacteria share a common signaling pathway in legumes .
Among the common symbiosis genes are CASTOR and POLLUX , the twin homologous genes in Lotus japonicus that encode putative ion channel proteins .
Here , we show that the orthologs of CASTOR and POLLUX are ubiquitously present and highly conserved in both legumes and nonlegumes .
Using rice ( Oryza sativa ) as a study system , we employ reverse genetic tools ( knockout mutants and RNA interference ) to demonstrate that Os-CASTOR and Os-POLLUX are indispensable for mycorrhizal symbiosis in rice .
Furthermore , a cross-species complementation test indicates that Os-POLLUX can restore nodulation , but not rhizobial infection , to a Medicago truncatula dmi1 mutant .
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[ Sen. 2, subscore: 1.00 ]: Among the common symbiosis genes are CASTOR and POLLUX , the twin homologous genes in Lotus japonicus that encode putative ion channel proteins .
[ Sen. 3, subscore: 1.00 ]: Here , we show that the orthologs of CASTOR and POLLUX are ubiquitously present and highly conserved in both legumes and nonlegumes .
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Score: 2.00 |
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| Title: Gene-based microsatellites for cassava ( Manihot esculenta Crantz ) : prevalence , polymorphisms , and cross-taxa utility .
| | Author: Raji AA Anderson JV Kolade OA Ugwu CD Dixon AG Ingelbrecht IL | | Journal: BMC Plant Biol Citation: V : 9 P : 118 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19747391 Accession (PMID): 19747391 | | Abstract: BACKGROUND : Cassava ( Manihot esculenta Crantz ) , a starchy root crop grown in tropical and subtropical climates , is the sixth most important crop in the world after wheat , rice , maize , potato and barley .
The repertoire of simple sequence repeat ( SSR ) markers for cassava is limited and warrants a need for a larger number of polymorphic SSRs for germplasm characterization and breeding applications .
RESULTS : A total of 846 putative microsatellites were identified in silico from an 8 , 577 cassava unigene set with an average density of one SSR every 7 kb .
One hundred and ninety-two candidate SSRs were screened for polymorphism among a panel of cassava cultivars from Africa , Latin America and Asia , four wild Manihot species as well as two other important taxa in the Euphorbiaceae , leafy spurge ( Euphorbia esula ) and castor bean ( Ricinus communis ) .
Of 168 markers with clean amplification products , 124 ( 73 . 8% ) displayed polymorphism based on high resolution agarose gels .
Of 85 EST-SSR markers screened , 80 ( 94 . 1% ) amplified alleles from one or more wild species ( M epruinosa , M glaziovii , M brachyandra , M tripartita ) whereas 13 ( 15 . 3% ) amplified alleles from castor bean and 9 ( 10 . 6% ) amplified alleles from leafy spurge ; hence nearly all markers were transferable to wild relatives of M esculenta while only a fraction was transferable to the more distantly related taxa .
In a subset of 20 EST-SSRs assessed by fluorescence-based genotyping the number of alleles per locus ranged from 2 to 10 with an average of 4 . 55 per locus .
These markers had a polymorphism information content ( PIC ) from 0 . 19 to 0 . 75 with an average value of 0 . 55 and showed genetic relationships consistent with existing information on these genotypes .
CONCLUSION : A set of 124 new , unique polymorphic EST-SSRs was developed and characterized which extends the repertoire of SSR markers for cultivated cassava and its wild relatives .
The markers show high PIC values and therefore will be useful for cultivar identification , taxonomic studies , and genetic mapping .
The study further shows that mining ESTs is a highly efficient strategy for polymorphism detection within the cultivated cassava gene pool .
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[ Sen. 4, subscore: 1.00 ]: One hundred and ninety-two candidate SSRs were screened for polymorphism among a panel of cassava cultivars from Africa , Latin America and Asia , four wild Manihot species as well as two other important taxa in the Euphorbiaceae , leafy spurge ( Euphorbia esula ) and castor bean ( Ricinus communis ) .
[ Sen. 6, subscore: 1.00 ]: Of 85 EST-SSR markers screened , 80 ( 94 . 1% ) amplified alleles from one or more wild species ( M epruinosa , M glaziovii , M brachyandra , M tripartita ) whereas 13 ( 15 . 3% ) amplified alleles from castor bean and 9 ( 10 . 6% ) amplified alleles from leafy spurge ; hence nearly all markers were transferable to wild relatives of M esculenta while only a fraction was transferable to the more distantly related taxa .
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Score: 2.00 |
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| Title: Identification and expression profiles of the WRKY transcription factor family in Ricinus communis .
| | Author: Li HL Zhang LB Guo D Li CZ Peng SQ | | Journal: Gene Citation: V : 503 P : 248-53 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22579867 Accession (PMID): 22579867 | | Abstract: In plants , WRKY proteins constitute a large family of transcription factors .
They are involved in many biological processes , such as plant development , metabolism , and responses to biotic and abiotic stresses .
A large number of WRKY transcription factors have been reported from Arabidopsis , rice , and other higher plants .
The recent publication of the draft genome sequence of castor bean ( Ricinus communis ) has allowed a genome-wide search for R communis WRKY ( RcWRKY ) transcription factors and the comparison of these positively identified proteins with their homologs in model plants .
A total of 47 WRKY genes were identified in the castor bean genome .
According to the structural features of the WRKY domain , the RcWRKY are classified into seven main phylogenetic groups .
Furthermore , putative orthologs of RcWRKY proteins in Arabidopsis and rice could now be assigned .
An analysis of expression profiles of RcWRKY genes indicates that 47 WRKY genes display differential expressions either in their transcript abundance or expression patterns under normal growth conditions .
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[ Sen. 4, subscore: 1.00 ]: The recent publication of the draft genome sequence of castor bean ( Ricinus communis ) has allowed a genome-wide search for R communis WRKY ( RcWRKY ) transcription factors and the comparison of these positively identified proteins with their homologs in model plants .
[ Sen. 5, subscore: 1.00 ]: A total of 47 WRKY genes were identified in the castor bean genome .
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Score: 1.00 |
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| Title: Phosphoenolpyruvate carboxylase protein kinase from developing castor oil seeds : partial purification , characterization , and reversible control by photosynthate supply .
| | Author: Murmu J Plaxton WC | | Journal: Planta Citation: V : 226 P : 1299-310 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17624549 Accession (PMID): 17624549 | | Abstract: Phosphoenolpyruvate carboxylase ( PEPC , EC 4 . 1 . 1 . 31 ) protein kinase ( PPCK ) was purified approximately 1 , 500-fold from developing castor oil seeds ( COS ) .
Gel filtration and immunoblotting with anti- ( rice PPCK2 ) -immune serum indicated that this Ca2+-insensitive PPCK exists as a 31-kDa monomer .
COS PPCK-mediated rephosphorylation of the 107-kDa subunit ( p107 ) of COS PEPC1 ( Km = 2 . 2 microM ) activated PEPC1 by approximately 80% when assayed under suboptimal conditions ( pH 7 . 3 , 0 . 2 mM PEP , and 0 . 125 mM malate ) .
COS PPCK displayed remarkable selectivity for phosphorylating COS PEPC1 ( relative to tobacco , sorghum , or maize PEPCs ) , exhibited a broad pH-activity optima of approximately pH 8 . 5 , and at pH 7 . 3 was activated 40-65% by 1 mM PEP , or 10 mM Gln or Asn , but inhibited 65% by 10 mM L-malate .
The possible control of COS PPCK by disulfide-dithiol interconversion was suggested by its rapid inactivation and subsequent reactivation when incubated with oxidized glutathione and then dithiothreitol .
In vitro PPCK activity correlated with in vivo p107 phosphorylation status , with both peaking in mid-cotyledon to full-cotyledon developing COS .
Notably , PPCK activity and p107 phosphorylation of developing COS were eliminated following pod excision or prolonged darkness of intact plants .
Both effects were fully reversed 12 h following reillumination of darkened plants .
These results implicate a direct relationship between the up-regulation of COS PPCK and p107 phosphorylation during the recommencement of photosynthate delivery from illuminated leaves to the non-photosynthetic COS .
Overall , the results support the hypothesis that PEPC and PPCK participate in the control of photosynthate partitioning into C-skeletons needed as precursors for key biosynthetic pathways of developing COS .
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[ Sen. 1, subscore: 1.00 ]: Phosphoenolpyruvate carboxylase ( PEPC , EC 4 . 1 . 1 . 31 ) protein kinase ( PPCK ) was purified approximately 1 , 500-fold from developing castor oil seeds ( COS ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
