| 6 matches found in 4 documents. Search time: 0.715 seconds. |
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| Title: A rice cab gene promoter contains separate cis-acting elements that regulate expression in dicot and monocot plants .
| | Author: Luan S Bogorad L | | Journal: Plant Cell Citation: V : 4 ( 8 ) P : 971-81 Year: 1992 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1392604 Accession (PMID): 1392604 | | Abstract: The major light-harvesting chlorophyll a/b binding proteins of the photosynthetic apparatus are encoded by families of nuclear cab genes .
The expression of most cab genes is it issue specific and photoregulated in angiosperms .
In transgenic tobacco plants , expression of the reporter gene beta-glucuronidase ( GUS ) is photoregulated and it issue specific from 5 upstream sequences of the rice cab1R gene ; deletion of sequences upstream from position -170 with respect to the transcription start site eliminates the enhanced and photoregulated expression in the transgenic plants .
Using an in situ transient expression assay , we have determined that the sequence OCT-R , an octamer repeat that lies within the -269 to -170 region of cab1R , is essential for photoregulated expression of the chimeric GUS gene in leaf cells of maize and rice but is not required for expression in illuminated tobacco leaves .
Conversely , box III*- and G-box-like sequences found near OCT-R in cab1R are necessary for high-level transient expression of the reporter gene in tobacco leaf it issue but are not required for transient expression in maize or rice leaves . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: In transgenic tobacco plants , expression of the reporter gene beta-glucuronidase ( GUS ) is photoregulated and it issue specific from 5 upstream sequences of the rice cab1R gene ; deletion of sequences upstream from position -170 with respect to the transcription start site eliminates the enhanced and photoregulated expression in the transgenic plants .
[ Sen. 4, subscore: 1.00 ]: Using an in situ transient expression assay , we have determined that the sequence OCT-R , an octamer repeat that lies within the -269 to -170 region of cab1R , is essential for photoregulated expression of the chimeric GUS gene in leaf cells of maize and rice but is not required for expression in illuminated tobacco leaves .
[ Sen. 5, subscore: 1.00 ]: Conversely , box III*- and G-box-like sequences found near OCT-R in cab1R are necessary for high-level transient expression of the reporter gene in tobacco leaf it issue but are not required for transient expression in maize or rice leaves .
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| Title: Light regulation of circadian clock-controlled gene expression in rice .
| | Author: Sugiyama N Izawa T Oikawa T Shimamoto K | | Journal: Plant J Citation: V : 26 ( 6 ) P : 607-15 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11489174 Accession (PMID): 11489174 | | Abstract: Using transgenic rice seedlings expressing a firefly luciferase ( luc ) gene under the control of a rice CAB ( chlorophyll a/b binding protein ) promoter , we demonstrated how light affects circadian clocks in the transcription of the CAB gene .
Rhythmic luc expression was observed for more than 5 d under constant light and dark ( DD ) conditions after light/dark entrainment .
After a light pulse was applied at different time points in DD various temporal patterns of CAB gene expression were individually observed .
We first examined two distinct properties related to the entrainment mechanism of the circadian clock : fluence-rate dependence of free-running periods ( FRPs ) and phase resetting by a light pulse .
Although fluence-rate dependent shortening of FRP was demonstrated , the FRP in DD was almost equal to that in constant light of a middle fluence-rate , indicating that this fluence-rate dependence may not fully describe the entrainment of the circadian clock in rice .
Typical phase responses of the circadian clock by a single light pulse were also observed at the transcriptional level in rice seedlings .
Thus , the phase resettings upon the light/dark transitions of daily cycles may be sufficient to explain the entrainment mechanisms of rice .
We have further demonstrated that , in addition to having a gating effect to acute response , a light pulse can activate the circadian clock-controlled CAB1R gene expression at the first circadian peak in a phase-dependent manner .
This suggests that light activates circadian clock activity in the diurnal CAB gene expression under daily light/dark cycles . | Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: We have further demonstrated that , in addition to having a gating effect to acute response , a light pulse can activate the circadian clock-controlled CAB1R gene expression at the first circadian peak in a phase-dependent manner .
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| Title: A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis .
| | Author: Wu Z Zhang X He B Diao L Sheng S Wang J Guo X Su N Wang L Jiang L Wang C Zhai H Wan J | | Journal: Plant Physiol Citation: V : 145 P : 29-40 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17535821 Accession (PMID): 17535821 | | Abstract: Chlorophyll ( Chl ) synthase catalyzes esterification of chlorophyllide to complete the last step of Chl biosynthesis .
Although the Chl synthases and the corresponding genes from various organisms have been well characterized , Chl synthase mutants have not yet been reported in higher plants .
In this study , a rice ( Oryza Sativa ) Chl-deficient mutant , yellow-green leaf1 ( ygl1 ) , was isolated , which showed yellow-green leaves in young plants with decreased Chl synthesis , increased level of tetrapyrrole intermediates , and delayed chloroplast development .
Genetic analysis demonstrated that the phenotype of ygl1 was caused by a recessive mutation in a nuclear gene .
The ygl1 locus was mapped to chromosome 5 and isolated by map-based cloning .
Sequence analysis revealed that it encodes the Chl synthase and its identity was verified by transgenic complementation .
A missense mutation was found in a highly conserved residue of YGL1 in the ygl1 mutant , resulting in reduction of the enzymatic activity .
YGL1 is constitutively expressed in all it issues , and its expression is not significantly affected in the ygl1 mutant .
Interestingly , the mRNA expression of the cab1R gene encoding the Chl a/b-binding protein was severely suppressed in the ygl1 mutant .
Moreover , the expression of some nuclear genes associated with Chl biosynthesis or chloroplast development was also affected in ygl1 seedlings .
These results indicate that the expression of nuclear genes encoding various chloroplast proteins might be feedback regulated by the level of Chl or Chl precursors .
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[ Sen. 9, subscore: 1.00 ]: Interestingly , the mRNA expression of the cab1R gene encoding the Chl a/b-binding protein was severely suppressed in the ygl1 mutant .
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| Title: A rice stromal processing peptidase regulates chloroplast and root development .
| | Author: Yue R Wang X Chen J Ma X Zhang H Mao C Wu P | | Journal: Plant Cell Physiol Citation: V : 51 P : 475-85 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20097911 Accession (PMID): 20097911 | | Abstract: The stromal processing peptidase ( SPP ) is a metalloendopeptidase that cleaves a broad range of precursor substrates .
In this study , we isolated a rice mutant showing leaf chlorosis at the early seedling stage but inhibition of root growth during the whole growth period .
Genetic analysis demonstrates that the phenotypes of the mutant were caused by a recessive single gene mutation .
The mutated gene was cloned by map-based cloning , and was shown to encode an SPP .
Sequence analysis showed a glutamate deletion in the highly conserved C-terminus of SPP in the mutant .
The mutation of SPP in the mutant was verified by transgenic complementation .
SPP is constitutively expressed in all it issues .
Subcellular localization analysis indicates that SPP is targeted to the chloroplast The expression of some genes associated with chloroplast development was decreased in young seedlings of the spp mutant , but not in 14-day-old seedlings .
Western blot analysis revealed that the Rubisco small subunit is not precisely processed in the spp mutant in 7-day-old seedlings , but the processing activity in the spp mutant is restored in 14-day-old seedlings .
Moreover , the expression levels of Cab1R and Cab2R for the light-harvesting chlorophyll a/b-binding protein ( LHCP ) were highly up-regulated in the transgenic plants with overexpression of SPP .
The present results reveal that SPP is essential for chloroplast biogenesis at the early growth stage and for rice root development ; this is the first report on the function of SPP in monocot plants .
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[ Sen. 10, subscore: 1.00 ]: Moreover , the expression levels of Cab1R and Cab2R for the light-harvesting chlorophyll a/b-binding protein ( LHCP ) were highly up-regulated in the transgenic plants with overexpression of SPP .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
