Score: 9.00 |
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| Title: BRASSINOSTEROID UPREGULATED1 , encoding a helix-loop-helix protein , is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice .
| | Author: Tanaka A Nakagawa H Tomita C Shimatani Z Ohtake M Nomura T Jiang CJ Dubouzet JG Kikuchi S Sekimoto H Yokota T Asami T Kamakura T Mori M | | Journal: Plant Physiol Citation: V : 151 P : 669-80 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19648232 Accession (PMID): 19648232 | | Abstract: Brassinosteroids ( BRs ) are involved in many developmental processes and regulate many subsets of downstream genes throughout the plant kingdom .
However , little is known about the BR signal transduction and response network in monocots .
To identify novel BR-related genes in rice ( Oryza sativa ) , we monitored the transcriptomic response of the brassinosteroid deficient1 ( brd1 ) mutant , with a defective BR biosynthetic gene , to brassinolide treatment .
Here , we describe a novel BR-induced rice gene BRASSINOSTEROID UPREGULATED1 ( BU1 ) , encoding a helix-loop-helix protein .
Rice plants overexpressing BU1 ( BU1 : OX ) showed enhanced bending of the lamina joint , increased grain size , and resistance to brassinazole , an inhibitor of BR biosynthesis .
In contrast to BU1 : OX , RNAi plants designed to repress both BU1 and its homologs displayed erect leaves .
In addition , compared to the wild type , the induction of BU1 by exogenous brassinolide did not require de novo protein synthesis and it was weaker in a BR receptor mutant OsbriI ( Oryza sativa brassinosteroid insensitive1 , d61 ) and a rice G protein alpha subunit ( RGA1 ) mutant d1 .
These results indicate that BU1 protein is a positive regulator of BR response : it controls bending of the lamina joint in rice and it is a novel primary response gene that participates in two BR signaling pathways through OsBRI1 and RGA1 .
Furthermore , expression analyses showed that BU1 is expressed in several organs including lamina joint , phloem , and epithelial cells in embryos .
These results indicate that BU1 may participate in some other unknown processes modulated by BR in rice .
| Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Rice plants overexpressing BU1 ( BU1 : OX ) showed enhanced bending of the lamina joint , increased grain size , and resistance to brassinazole , an inhibitor of BR biosynthesis .
[ Sen. 6, subscore: 2.00 ]: In contrast to BU1 : OX , RNAi plants designed to repress both BU1 and its homologs displayed erect leaves .
[ Sen. 4, subscore: 1.00 ]: Here , we describe a novel BR-induced rice gene BRASSINOSTEROID UPREGULATED1 ( BU1 ) , encoding a helix-loop-helix protein .
[ Sen. 7, subscore: 1.00 ]: In addition , compared to the wild type , the induction of BU1 by exogenous brassinolide did not require de novo protein synthesis and it was weaker in a BR receptor mutant OsbriI ( Oryza sativa brassinosteroid insensitive1 , d61 ) and a rice G protein alpha subunit ( RGA1 ) mutant d1 .
[ Sen. 8, subscore: 1.00 ]: These results indicate that BU1 protein is a positive regulator of BR response : it controls bending of the lamina joint in rice and it is a novel primary response gene that participates in two BR signaling pathways through OsBRI1 and RGA1 .
[ Sen. 9, subscore: 1.00 ]: Furthermore , expression analyses showed that BU1 is expressed in several organs including lamina joint , phloem , and epithelial cells in embryos .
[ Sen. 10, subscore: 1.00 ]: These results indicate that BU1 may participate in some other unknown processes modulated by BR in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: H3K36 methylation is critical for brassinosteroid-regulated plant growth and development in rice .
| | Author: Sui P Jin J Ye S Mu C Gao J Feng H Shen WH Yu Y Dong A | | Journal: Plant J Citation: V : 70 P : 340-7 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22136623 Accession (PMID): 22136623 | | Abstract: Methylation of histone lysine residues plays an essential role in epigenetic regulation of gene expression in eukaryotes .
Enzymes involved in establishment of the repressive H3K9 and H3K27 methylation marks have been previously characterized , but the deposition and function of H3K4 and H3K36 methylation remain uncharacterized in rice .
Here , we report that rice SDG725 encodes a H3K36 methyltransferase , and its down-regulation causes wide-ranging defects , including dwarfism , shortened internodes , erect leaves and small seeds .
These defects resemble the phenotypes previously described for some brassinosteroid-knockdown mutants .
Consistently , transcriptome analyses revealed that SDG725 depletion results in down-regulation by more than two-fold of over 1000 genes , including D11 , BRI1 and BU1 , which are known to be involved in brassinosteroid biosynthesis or signaling pathways .
Chromatin immunoprecipitation analyses showed that levels of H3K36me2/3 are reduced in chromatin at some regions of these brassinosteroid-related genes in SDG725 knockdown plants , and that SDG725 protein is able to directly bind to these target genes .
Taken together , our data indicate that SDG725-mediated H3K36 methylation modulates brassinosteroid-related gene expression , playing an important role in rice plant growth and development .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Consistently , transcriptome analyses revealed that SDG725 depletion results in down-regulation by more than two-fold of over 1000 genes , including D11 , BRI1 and BU1 , which are known to be involved in brassinosteroid biosynthesis or signaling pathways .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
