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Enter keyword(s) and/or category/ies. Selecting categories for a query makes a search more specific. For example, you can retrieve sentences that contain the word HSN and a
Oryza sativa
gene name by typing the keyword 'SPW1' and choosing the category 'gene (Oryza sativa)'. A category hit occurs when a particular word or phrase in the sentence is defined as a member of a particular category. Categories will be concatenated by a Boolean 'and' operation to other categories and keyword(s) if present. To search for terms in categories, click on the Categories/Ontology link above.
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Title:
High-resolution mapping of a new brown planthopper ( BPH ) resistance gene , Bph18 ( t ) , and marker-assisted selection for BPH resistance in rice ( Oryza sativa L ) .
Author:
Jena KK Jeung JU Lee JH Choi HC Brar DS .
Journal:
Theor . Appl . Genet .
Citation:
V : 112 ( 2 ) P : 288-97
Year:
2006
Type:
ARTICLE
Literature:
oryza
Field:
abstract
Doc ID:
pub16240104
Accession (PMID):
16240104
Abstract:
Brown planthopper ( BPH ) is a destructive insect pest of rice in Asia . Identification and the incorporation of new BPH resistance genes into modern rice cultivars are important breeding strategies to control the damage caused by new biotypes of BPH . In this study , a major resistance gene , Bph18 ( t ) , has been identified in an introgression line ( IR65482-7-216-1-2 ) that has inherited the gene from the wild species Oryza australiensis . Genetic analysis revealed the dominant nature of the Bph18 ( t ) gene and identified it as non-allelic to another gene ,
Bph10
that was earlier introgressed from O australiensis . After linkage analysis using MapMaker followed by single-locus ANOVA on quantitatively expressed resistance levels of the progenies from an F2 mapping population identified with marker allele types , the Bph18 ( t ) gene was initially located on the subterminal region of the long arm of chromosome 12 flanked by the SSR marker RM463 and the STS marker S15552 . The corresponding physical region was identified in the Nipponbare genome pseudomolecule 3 through electronic chromosome landing ( e-landing ) , in which 15 BAC clones covered 1 . 612 Mb . Eleven DNA markers tagging the BAC clones were used to construct a high-resolution genetic map of the target region . The Bph18 ( t ) locus was further localized within a 0 . 843-Mb physical interval that includes three BAC clones between the markers R10289S and RM6869 by means of single-locus ANOVA of resistance levels of mapping population and marker-gene association analysis on 86 susceptible F2 progenies based on six time-point phenotyping . Using gene annotation information of TIGR , a putative resistance gene was identified in the BAC clone OSJNBa0028L05 and the sequence information was used to generate STS marker 7312 . T4A . The marker allele of 1 , 078 bp completely co-segregated with the BPH resistance phenotype . STS marker 7312 . T4A was validated using BC2F2 progenies derived from two temperate japonica backgrounds . Some 97 resistant BC2F2 individuals out of 433 screened completely co-segregated with the resistance-specific marker allele ( 1 , 078 bp ) in either homozygous or heterozygous state . This further confirmed a major gene-controlled resistance to the BPH biotype of Korea . Identification of Bph18 ( t ) enlarges the BPH resistance gene pool to help develop improved rice cultivars , and the PCR marker ( 7312 . T4A ) for the Bph18 ( t ) gene should be readily applicable for marker-assisted selection ( MAS ) .
Matching Sentences:
[ Sen. 4, subscore: 1.00 ]:
Genetic analysis revealed the dominant nature of the Bph18 ( t ) gene and identified it as non-allelic to another gene ,
Bph10
that was earlier introgressed from O australiensis .
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