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Score: 16.00 |
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| Title: Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6 .
| | Author: Qiu Y Guo J Jing S Zhu L He G | | Journal: Theor Appl Genet Citation: V : 124 P : 485-94 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22038433 Accession (PMID): 22038433 | | Abstract: The brown planthopper ( Nilaparvata lugens Stal ; BPH ) has become a severe constraint on rice production .
Identification and pyramiding BPH-resistance genes is an economical and effective solution to increase the resistance level of rice varieties .
All the BPH-resistance genes identified to date have been from indica rice or wild species .
The BPH12 gene in the indica rice accession B14 is derived from the wild species Oryza latifolia .
Using an F ( 2 ) population from a cross between the indica cultivar 93-11 and B14 , we mapped the BPH12 gene to a 1 . 9-cM region on chromosome 4 , flanked by the markers RM16459 and RM1305 .
In this population , BPH12 appeared to be partially dominant and explained 73 . 8% of the phenotypic variance in BPH resistance .
A near-isogenic line ( NIL ) containing the BPH12 locus in the background of the susceptible japonica variety Nipponbare was developed and crossed with a NIL carrying BPH6 to generate a pyramid line ( PYL ) with both genes .
BPH insects showed significant differences in non-preference in comparisons between the lines harboring resistance genes ( NILs and PYL ) and Nipponbare .
BPH growth and development were inhibited and survival rates were lower on the NIL-BPH12 and NIL-BPH6 plants compared to the recurrent parent Nipponbare .
PYL-BPH6 + BPH12 exhibited 46 . 4 , 26 . 8 and 72 . 1% reductions in population growth rates ( PGR ) compared to NIL-BPH12 , NIL-BPH6 and Nipponbare , respectively .
Furthermore , insect survival rates were the lowest on the PYL-BPH6 + BPH12 plants .
These results demonstrated that pyramiding different BPH-resistance genes resulted in stronger antixenotic and antibiotic effects on the BPH insects .
This gene pyramiding strategy should be of great benefit for the breeding of BPH-resistant japonica rice varieties .
| Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: In this population , BPH12 appeared to be partially dominant and explained 73 . 8% of the phenotypic variance in BPH resistance .
[ Sen. 7, subscore: 2.00 ]: A near-isogenic line ( NIL ) containing the BPH12 locus in the background of the susceptible japonica variety Nipponbare was developed and crossed with a NIL carrying BPH6 to generate a pyramid line ( PYL ) with both genes .
[ Sen. 12, subscore: 2.00 ]: These results demonstrated that pyramiding different BPH-resistance genes resulted in stronger antixenotic and antibiotic effects on the BPH insects .
[ Sen. 1, subscore: 1.00 ]: The brown planthopper ( Nilaparvata lugens Stal ; BPH ) has become a severe constraint on rice production .
[ Sen. 2, subscore: 1.00 ]: Identification and pyramiding BPH-resistance genes is an economical and effective solution to increase the resistance level of rice varieties .
[ Sen. 3, subscore: 1.00 ]: All the BPH-resistance genes identified to date have been from indica rice or wild species .
[ Sen. 4, subscore: 1.00 ]: The BPH12 gene in the indica rice accession B14 is derived from the wild species Oryza latifolia .
[ Sen. 5, subscore: 1.00 ]: Using an F ( 2 ) population from a cross between the indica cultivar 93-11 and B14 , we mapped the BPH12 gene to a 1 . 9-cM region on chromosome 4 , flanked by the markers RM16459 and RM1305 .
[ Sen. 8, subscore: 1.00 ]: BPH insects showed significant differences in non-preference in comparisons between the lines harboring resistance genes ( NILs and PYL ) and Nipponbare .
[ Sen. 9, subscore: 1.00 ]: BPH growth and development were inhibited and survival rates were lower on the NIL-BPH12 and NIL-BPH6 plants compared to the recurrent parent Nipponbare .
[ Sen. 10, subscore: 1.00 ]: PYL-BPH6 + BPH12 exhibited 46 . 4 , 26 . 8 and 72 . 1% reductions in population growth rates ( PGR ) compared to NIL-BPH12 , NIL-BPH6 and Nipponbare , respectively .
[ Sen. 11, subscore: 1.00 ]: Furthermore , insect survival rates were the lowest on the PYL-BPH6 + BPH12 plants .
[ Sen. 13, subscore: 1.00 ]: This gene pyramiding strategy should be of great benefit for the breeding of BPH-resistant japonica rice varieties .
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Score: 15.00 |
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| Title: High-resolution mapping of a new brown planthopper ( BPH ) resistance gene , Bph18 ( t ) , and marker-assisted selection for BPH resistance in rice ( Oryza sativa L ) .
| | Author: Jena KK Jeung JU Lee JH Choi HC Brar DS .
| | Journal: Theor . Appl . Genet . Citation: V : 112 ( 2 ) P : 288-97 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16240104 Accession (PMID): 16240104 | | Abstract: Brown planthopper ( BPH ) is a destructive insect pest of rice in Asia .
Identification and the incorporation of new BPH resistance genes into modern rice cultivars are important breeding strategies to control the damage caused by new biotypes of BPH .
In this study , a major resistance gene , Bph18 ( t ) , has been identified in an introgression line ( IR65482-7-216-1-2 ) that has inherited the gene from the wild species Oryza australiensis .
Genetic analysis revealed the dominant nature of the Bph18 ( t ) gene and identified it as non-allelic to another gene , Bph10 that was earlier introgressed from O australiensis .
After linkage analysis using MapMaker followed by single-locus ANOVA on quantitatively expressed resistance levels of the progenies from an F2 mapping population identified with marker allele types , the Bph18 ( t ) gene was initially located on the subterminal region of the long arm of chromosome 12 flanked by the SSR marker RM463 and the STS marker S15552 .
The corresponding physical region was identified in the Nipponbare genome pseudomolecule 3 through electronic chromosome landing ( e-landing ) , in which 15 BAC clones covered 1 . 612 Mb .
Eleven DNA markers tagging the BAC clones were used to construct a high-resolution genetic map of the target region .
The Bph18 ( t ) locus was further localized within a 0 . 843-Mb physical interval that includes three BAC clones between the markers R10289S and RM6869 by means of single-locus ANOVA of resistance levels of mapping population and marker-gene association analysis on 86 susceptible F2 progenies based on six time-point phenotyping .
Using gene annotation information of TIGR , a putative resistance gene was identified in the BAC clone OSJNBa0028L05 and the sequence information was used to generate STS marker 7312 . T4A .
The marker allele of 1 , 078 bp completely co-segregated with the BPH resistance phenotype .
STS marker 7312 . T4A was validated using BC2F2 progenies derived from two temperate japonica backgrounds .
Some 97 resistant BC2F2 individuals out of 433 screened completely co-segregated with the resistance-specific marker allele ( 1 , 078 bp ) in either homozygous or heterozygous state .
This further confirmed a major gene-controlled resistance to the BPH biotype of Korea .
Identification of Bph18 ( t ) enlarges the BPH resistance gene pool to help develop improved rice cultivars , and the PCR marker ( 7312 . T4A ) for the Bph18 ( t ) gene should be readily applicable for marker-assisted selection ( MAS ) . | Matching Sentences:
[ Sen. 14, subscore: 3.00 ]: Identification of Bph18 ( t ) enlarges the BPH resistance gene pool to help develop improved rice cultivars , and the PCR marker ( 7312 . T4A ) for the Bph18 ( t ) gene should be readily applicable for marker-assisted selection ( MAS ) .
[ Sen. 2, subscore: 2.00 ]: Identification and the incorporation of new BPH resistance genes into modern rice cultivars are important breeding strategies to control the damage caused by new biotypes of BPH .
[ Sen. 4, subscore: 2.00 ]: Genetic analysis revealed the dominant nature of the Bph18 ( t ) gene and identified it as non-allelic to another gene , Bph10 that was earlier introgressed from O australiensis .
[ Sen. 10, subscore: 2.00 ]: The marker allele of 1 , 078 bp completely co-segregated with the BPH resistance phenotype .
[ Sen. 1, subscore: 1.00 ]: Brown planthopper ( BPH ) is a destructive insect pest of rice in Asia .
[ Sen. 3, subscore: 1.00 ]: In this study , a major resistance gene , Bph18 ( t ) , has been identified in an introgression line ( IR65482-7-216-1-2 ) that has inherited the gene from the wild species Oryza australiensis .
[ Sen. 5, subscore: 1.00 ]: After linkage analysis using MapMaker followed by single-locus ANOVA on quantitatively expressed resistance levels of the progenies from an F2 mapping population identified with marker allele types , the Bph18 ( t ) gene was initially located on the subterminal region of the long arm of chromosome 12 flanked by the SSR marker RM463 and the STS marker S15552 .
[ Sen. 8, subscore: 1.00 ]: The Bph18 ( t ) locus was further localized within a 0 . 843-Mb physical interval that includes three BAC clones between the markers R10289S and RM6869 by means of single-locus ANOVA of resistance levels of mapping population and marker-gene association analysis on 86 susceptible F2 progenies based on six time-point phenotyping .
[ Sen. 12, subscore: 1.00 ]: Some 97 resistant BC2F2 individuals out of 433 screened completely co-segregated with the resistance-specific marker allele ( 1 , 078 bp ) in either homozygous or heterozygous state .
[ Sen. 13, subscore: 1.00 ]: This further confirmed a major gene-controlled resistance to the BPH biotype of Korea .
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Score: 14.00 |
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| Title: Heritability of blood pressure responses to cold pressor test in a Chinese population .
| | Author: Mei H Gu D Rice TK Hixson JE Chen J Jaquish CE Zhao Q Chen CS Chen JC Gu CC Kelly TN He J | | Journal: Am J Hypertens Citation: V : 22 P : 1096-100 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19661924 Accession (PMID): 19661924 | | Abstract: BACKGROUND : Genetic determinants of blood pressure ( BP ) responses to the cold pressor test ( CPT ) , a phenotype associated with risk of hypertension and cardiovascular disease has not been well studied .
METHODS : We examined the heritability of BP response to CPT in 1 , 994 subjects from 627 families in rural north China .
BP was measured before and at 0 , 1 , 2 , and 4 min after the participants immersed their hand in ice water for 1 min .
Heritabilities of baseline BP and responses at 0 min , maximum response , and area-under-the-curve ( AUC ) during CPT were computed using a variance components method .
Additionally , bivariate heritabilities were calculated to test the existence of shared genetic determinants between baseline BP and responses to CPT .
RESULTS : Heritabilities of baseline BP and responses to CPT were estimated from 14 to 35% , which all significantly differed from 0 ( P < or = 0 . 002 ) .
Genetic correlations ( s . e . ) due to the same genes between baseline BP and responses to CPT ranged from -0 . 07 ( 0 . 14 ) to 0 . 21 ( 0 . 15 ) , which were not significantly different from 0 .
Genetic correlations between reactivity and recovery were 0 . 67 ( 0 . 10 ) and 0 . 59 ( 0 . 10 ) for systolic ( SBP ) and diastolic BP ( DBP ) , respectively , which were significantly different from 0 .
CONCLUSIONS : We concluded that ( i ) baseline BP and BP responses to CPT had strong genetic determinants ; ( ii ) baseline BP and BP response to CPT did not share the same genetic components ; and ( iii ) BP reactivity and recovery shared the same genetic components .
These findings may lead to a better understanding of the genetic mechanism of BP responses to CPT .
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[ Sen. 9, subscore: 5.00 ]: CONCLUSIONS : We concluded that ( i ) baseline BP and BP responses to CPT had strong genetic determinants ; ( ii ) baseline BP and BP response to CPT did not share the same genetic components ; and ( iii ) BP reactivity and recovery shared the same genetic components .
[ Sen. 1, subscore: 1.00 ]: BACKGROUND : Genetic determinants of blood pressure ( BP ) responses to the cold pressor test ( CPT ) , a phenotype associated with risk of hypertension and cardiovascular disease has not been well studied .
[ Sen. 2, subscore: 1.00 ]: METHODS : We examined the heritability of BP response to CPT in 1 , 994 subjects from 627 families in rural north China .
[ Sen. 3, subscore: 1.00 ]: BP was measured before and at 0 , 1 , 2 , and 4 min after the participants immersed their hand in ice water for 1 min .
[ Sen. 4, subscore: 1.00 ]: Heritabilities of baseline BP and responses at 0 min , maximum response , and area-under-the-curve ( AUC ) during CPT were computed using a variance components method .
[ Sen. 5, subscore: 1.00 ]: Additionally , bivariate heritabilities were calculated to test the existence of shared genetic determinants between baseline BP and responses to CPT .
[ Sen. 6, subscore: 1.00 ]: RESULTS : Heritabilities of baseline BP and responses to CPT were estimated from 14 to 35% , which all significantly differed from 0 ( P < or = 0 . 002 ) .
[ Sen. 7, subscore: 1.00 ]: Genetic correlations ( s . e . ) due to the same genes between baseline BP and responses to CPT ranged from -0 . 07 ( 0 . 14 ) to 0 . 21 ( 0 . 15 ) , which were not significantly different from 0 .
[ Sen. 8, subscore: 1.00 ]: Genetic correlations between reactivity and recovery were 0 . 67 ( 0 . 10 ) and 0 . 59 ( 0 . 10 ) for systolic ( SBP ) and diastolic BP ( DBP ) , respectively , which were significantly different from 0 .
[ Sen. 10, subscore: 1.00 ]: These findings may lead to a better understanding of the genetic mechanism of BP responses to CPT .
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Score: 14.00 |
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| Title: Biological effects of rice harbouring Bph14 and Bph15 on brown planthopper , Nilaparvata lugens .
| | Author: Li J Chen Q Wang L Liu J Shang K Hua H | | Journal: Pest Manag Sci Citation: V : 67 P : 528-34 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21254325 Accession (PMID): 21254325 | | Abstract: BACKGROUND : Brown planthopper ( Nilaparvata lugens Stal ; BPH ) resistance genes Bph14 and Bph15 have been introgressed singly or pyramided into rice variety Minghui 63 ( MH63 ) .
The antibiosis and antixenosis effects of these rice lines on BPH and the expression of five P450 genes of BPH regulated by these rice lines were investigated in this study .
RESULTS : The resistance level of rice lines harbouring resistance genes was improved compared with MH63 .
MH63 : : 14 ( carrying Bph14 ) had negative effects on the development of males , honeydew excretion of females , the female ratio and the copulation rate compared with MH63 .
MH63 : : 14 also exhibited antixenosis action against BPH nymphs , female adults and oviposition .
Besides these negative effects , MH63 : : 15 ( carrying Bph15 ) could also retard the development of females , lower the fecundity and shorten the lifespan of females .
The antixenosis action of MH63 : : 15 was stronger than that of MH63 : : 14 .
When Bph14 and Bph15 were pyramided , antibiosis and antixenosis effects were significantly enhanced relative to single-introgression lines .
Among the five P450 genes of BPH , expression of three genes was upregulated , one gene was downregulated and one gene was unchanged by resistant hosts .
CONCLUSION : Both Bph14 and Bph15 could improve resistance levels of MH63 .
MH63 : : 15 and MH63 : : 14&15 had greater potential to control BPH infestations than MH63 : : 14 .
| Matching Sentences:
[ Sen. 1, subscore: 3.00 ]: BACKGROUND : Brown planthopper ( Nilaparvata lugens Stal ; BPH ) resistance genes Bph14 and Bph15 have been introgressed singly or pyramided into rice variety Minghui 63 ( MH63 ) .
[ Sen. 2, subscore: 2.00 ]: The antibiosis and antixenosis effects of these rice lines on BPH and the expression of five P450 genes of BPH regulated by these rice lines were investigated in this study .
[ Sen. 8, subscore: 2.00 ]: When Bph14 and Bph15 were pyramided , antibiosis and antixenosis effects were significantly enhanced relative to single-introgression lines .
[ Sen. 10, subscore: 2.00 ]: CONCLUSION : Both Bph14 and Bph15 could improve resistance levels of MH63 .
[ Sen. 4, subscore: 1.00 ]: MH63 : : 14 ( carrying Bph14 ) had negative effects on the development of males , honeydew excretion of females , the female ratio and the copulation rate compared with MH63 .
[ Sen. 5, subscore: 1.00 ]: MH63 : : 14 also exhibited antixenosis action against BPH nymphs , female adults and oviposition .
[ Sen. 6, subscore: 1.00 ]: Besides these negative effects , MH63 : : 15 ( carrying Bph15 ) could also retard the development of females , lower the fecundity and shorten the lifespan of females .
[ Sen. 9, subscore: 1.00 ]: Among the five P450 genes of BPH , expression of three genes was upregulated , one gene was downregulated and one gene was unchanged by resistant hosts .
[ Sen. 11, subscore: 1.00 ]: MH63 : : 15 and MH63 : : 14&15 had greater potential to control BPH infestations than MH63 : : 14 .
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Score: 13.00 |
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| Title: High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds .
| | Author: Qiu Y Guo J Jing S Zhu L He G | | Journal: Theor Appl Genet Citation: V : 121 P : 1601-11 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20680611 Accession (PMID): 20680611 | | Abstract: Brown planthopper ( Nilaparvata lugens Stal , BPH ) is one of the most destructive insect pests of rice .
Exploring resistance genes from diverse germplasms and incorporating them into cultivated varieties are critical for controlling this insect .
The rice variety Swarnalata was reported to carry a resistance gene ( designated Bph6 ) , which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown .
In this study , we identified and mapped this gene using the F ( 2 ) and backcrossing populations and characterized its resistance in indica 9311 and japonica Nipponbare using near isogenic lines ( NILs ) .
In analysis of 9311/Swarnalata F ( 2 ) population , the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742 .
The gene was further mapped precisely to a 25-kb region delimited between the STS markers Y19 and Y9 ; and the distance between these markers is 25-kb in Nipponbare genome .
The Bph6 explained 77 . 5% of the phenotypic variance of BPH resistance in F ( 2 ) population and 84 . 9% in BC ( 2 ) F ( 2 ) population .
Allele from Swarnalata significantly increased resistance to the BPH , resulted in a reduced damage score .
In characterization of Bph6-mediated resistance , the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release .
BPH growth and development were inhibited , and the insects survival rates were lower on Bph6-NIL plants , compared with the parents 9311 and Nipponbare .
The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants , and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants .
| Matching Sentences:
[ Sen. 11, subscore: 3.00 ]: The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants , and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants .
[ Sen. 7, subscore: 2.00 ]: The Bph6 explained 77 . 5% of the phenotypic variance of BPH resistance in F ( 2 ) population and 84 . 9% in BC ( 2 ) F ( 2 ) population .
[ Sen. 9, subscore: 2.00 ]: In characterization of Bph6-mediated resistance , the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release .
[ Sen. 10, subscore: 2.00 ]: BPH growth and development were inhibited , and the insects survival rates were lower on Bph6-NIL plants , compared with the parents 9311 and Nipponbare .
[ Sen. 1, subscore: 1.00 ]: Brown planthopper ( Nilaparvata lugens Stal , BPH ) is one of the most destructive insect pests of rice .
[ Sen. 3, subscore: 1.00 ]: The rice variety Swarnalata was reported to carry a resistance gene ( designated Bph6 ) , which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown .
[ Sen. 5, subscore: 1.00 ]: In analysis of 9311/Swarnalata F ( 2 ) population , the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742 .
[ Sen. 8, subscore: 1.00 ]: Allele from Swarnalata significantly increased resistance to the BPH , resulted in a reduced damage score .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
