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Score: 2.00
Title: Statistical optimization of thermo-tolerant xylanase activity from Amazon isolated Bacillus circulans on solid-state cultivation .
Author: Heck JX Flres SH Hertz PF Ayub MA .
Journal: Bioresour . Technol . Citation: V : 97 ( 15 ) P : 1902-6 Year: 2006 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub16216495 Accession (PMID): 16216495
Abstract: A 2 ( 2 ) factorial design was performed to find the best conditions of pH and temperature for xylanolytic activity of Bacillus circulans BL53 isolated from the Amazon environment . Solid-state cultivation was carried out on an inexpensive , abundant agro-industrial soybean residue . The central composite design ( CCD ) used for the analysis of treatment combinations showed that a second-order polynomial regression model was in good agreement with experimental results , with R ( 2 ) = 0 . 9369 ( P < 0 . 05 ) . The maximum activity was obtained at a high temperature ( 80 degrees C ) and over a large pH range ( 4 . 0-7 . 0 ) . Enzymatic activity was maintained in heated extracts up to 50 degrees C , suggesting that the xylanases of B circulans BL53 are thermo-tolerant biocatalysts , being of interest for industrial processes . The crude enzyme extract hydrolyzed rice straw , sugar cane bagasse and soybean fiber and its activity was stimulated by Co ( 2+ ) , Fe ( 3+ ) , and beta-mercaptoethanol but inhibited by Mn ( 2+ ) , Cu ( 2+ ) , Ca ( 2+ ) , Zn ( 2+ ) , Ba ( 2+ ) , Mg ( 2+ ) and by EDTA .
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[ Sen. 1, subscore: 1.00 ]: A 2 ( 2 ) factorial design was performed to find the best conditions of pH and temperature for xylanolytic activity of Bacillus circulans BL53 isolated from the Amazon environment .
[ Sen. 5, subscore: 1.00 ]: Enzymatic activity was maintained in heated extracts up to 50 degrees C , suggesting that the xylanases of B circulans BL53 are thermo-tolerant biocatalysts , being of interest for industrial processes .
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