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Title: Solid state bioreactor production of transglutaminase by Amazonian Bacillus circulans BL32 strain .
Author: de Souza CF Heck JX Ayub MA
Journal: J Ind Microbiol Biotechnol Citation: V : 35 P : 1677-85 Year: 2008 Type: In-Data-Review
Literature: oryza Field: title Doc ID: pub18696133 Accession (PMID): 18696133
Abstract: In this work , we investigated the production of transglutaminase ( TGase ) by an Amazonian isolated strain of Bacillus circulans by solid-state cultivation ( SSC ) . Several agro-industrial residues , such as untreated corn grits , milled brewers rice , industrial fibrous soy residue , soy hull , and malt bagasse , were used as substrates for microbial growth and enzyme production . Growth on industrial fibrous soy residue , which is rich in protein and hemicellulose , produced the highest TGase activity ( 0 . 74 U g ( -1 ) of dried substrate after 48 h of incubation ) . A 2 ( 3 ) central composite design was applied to determine the optimal conditions of aeration , cultivation temperature and inoculum cell concentration to TGase production . The best culture conditions were determined as being 0 . 6 L air min ( -1 ) , 33 degrees C and 10 log ( 10 ) CFU g ( -1 ) of dried substrate , respectively . Under the proposed optimized conditions , the model predicted an enzyme production of 1 . 16 U g ( -1 ) of dried substrate , closely matching the experimental activity of 1 . 25 U g ( -1 ) . Results presented in this work point to the use of this newly isolated B circulans strain as a potential alternative of microbial source for TGase production by SSC , using inexpensive culture media .
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[ Sen. 1, subscore: 1.00 ]: Solid state bioreactor production of transglutaminase by Amazonian Bacillus circulans BL32 strain .
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