| 59 matches found in 24 documents. Search time: 0.22 seconds. |
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Score: 11.00 |
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| Title: Ds tagging of BRANCHED FLORETLESS 1 ( BFL1 ) that mediates the transition from spikelet to floret meristem in rice ( Oryza sativa L ) .
| | Author: Zhu QH Hoque MS Dennis ES Upadhyaya NM .
| | Journal: BMC Plant Biol .
Citation: V : 3 ( ) P : 6 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14503923 Accession (PMID): 14503923 | | Abstract: BACKGROUND : The genetics of spikelet formation , a feature unique to grasses such as rice and maize , is yet to be fully understood , although a number of meristem and organ identity mutants have been isolated and investigated in Arabidopsis and maize .
Using a two-element Ac/Ds transposon tagging system we have isolated a rice mutant , designated branched floretless 1 ( bfl1 ) which is defective in the transition from spikelet meristem to floret meristem .
RESULTS : The bfl1 mutant shows normal differentiation of the primary rachis-branches leading to initial spikelet meristem ( bract-like structure equivalent to rudimentary glumes ) formation but fails to develop empty glumes and florets .
Instead , axillary meristems in the bract-like structure produce sequential alternate branching , thus resulting in a coral shaped morphology of the branches in the developing panicle .
The bfl1 mutant harbours a single Ds insertion in the upstream region of the BFL1 gene on chromosome 7 corresponding to PAC clone P0625E02 ( GenBank Acc No message URL http : //www . ncbi . nlm . nih . gov/entrez/query . fcgi?cmd=Retrieve&db=nucleotide&list_uids=34395191&dopt=GenBank&term=ap004570AP004570 ) .
RT-PCR analyses revealed a drastic reduction of BFL1 transcript levels in the bfl1 mutant compared to that in the wild-type .
In each of the normal panicle-bearing progeny plants , from occasional revertant seeds of the vegetatively-propagated mutant plant , Ds was shown to be excised from the bfl1 locus .
BFL1 contains an EREBP/AP2 domain and is most likely an ortholog of the maize transcription factor gene BRANCHED SILKLESS1 ( BD1 ) .
CONCLUSIONS : bfl1 is a Ds-tagged rice mutant defective in the transition from spikelet meristem ( SM ) to floret meristem ( FM ) .
BFL1 is most probably a rice ortholog of the maize ERF ( EREBP/AP2 ) transcription factor gene BD1 .
Based on the similarities in mutant phenotypes bfl1 is likely to be an allele of the previously reported frizzy panicle locus . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: The bfl1 mutant harbours a single Ds insertion in the upstream region of the BFL1 gene on chromosome 7 corresponding to PAC clone P0625E02 ( GenBank Acc No message URL http : //www . ncbi . nlm . nih . gov/entrez/query . fcgi?cmd=Retrieve&db=nucleotide&list_uids=34395191&dopt=GenBank&term=ap004570AP004570 ) .
[ Sen. 6, subscore: 2.00 ]: RT-PCR analyses revealed a drastic reduction of BFL1 transcript levels in the bfl1 mutant compared to that in the wild-type .
[ Sen. 2, subscore: 1.00 ]: Using a two-element Ac/Ds transposon tagging system we have isolated a rice mutant , designated branched floretless 1 ( bfl1 ) which is defective in the transition from spikelet meristem to floret meristem .
[ Sen. 3, subscore: 1.00 ]: RESULTS : The bfl1 mutant shows normal differentiation of the primary rachis-branches leading to initial spikelet meristem ( bract-like structure equivalent to rudimentary glumes ) formation but fails to develop empty glumes and florets .
[ Sen. 7, subscore: 1.00 ]: In each of the normal panicle-bearing progeny plants , from occasional revertant seeds of the vegetatively-propagated mutant plant , Ds was shown to be excised from the bfl1 locus .
[ Sen. 8, subscore: 1.00 ]: BFL1 contains an EREBP/AP2 domain and is most likely an ortholog of the maize transcription factor gene BRANCHED SILKLESS1 ( BD1 ) .
[ Sen. 9, subscore: 1.00 ]: CONCLUSIONS : bfl1 is a Ds-tagged rice mutant defective in the transition from spikelet meristem ( SM ) to floret meristem ( FM ) .
[ Sen. 10, subscore: 1.00 ]: BFL1 is most probably a rice ortholog of the maize ERF ( EREBP/AP2 ) transcription factor gene BD1 .
[ Sen. 11, subscore: 1.00 ]: Based on the similarities in mutant phenotypes bfl1 is likely to be an allele of the previously reported frizzy panicle locus .
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Score: 6.00 |
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| Title: [ Observation for CH4 and N2O emissions under different rates of nitrogen and phosphate fertilization in double rice fields ] .
| | Author: Shi SW Li YE Wan YF Qin XB Gao QZ | | Journal: Huan Jing Ke Xue Citation: V : 32 P : 1899-907 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21922807 Accession (PMID): 21922807 | | Abstract: Two non-CO2 greenhouse gas emissions ( methane and nitrous oxide ) and related environmental factors were measured within rice growing season under five treatments including non-fertilization ( CK ) , balanced fertilization ( BF ) , decreased nitrogen and phosphate 1 ( DNP1 ) , decreased nitrogen and phosphate 2 ( DNP2 ) and increased nitrogen and phosphate 1 ( INP ) in double rice fields of red clay soil in 2009 , using the method of static chamber-gas chromatograph techniques .
The results showed that the average CH4 emission fluxes for treatments of BF , DNP1 , DNP2 and INP were 4 . 57 , 5 . 42 , 4 . 70 and 4 . 65 mg x ( m2 x h ) ( -1 ) during early rice growing period , which increased by 39% , 49% , 41% and 40% compared with non-fertilizer treatment , respectively .
The average CH4 emission fluxes in late rice growing season was higher than preseasons .
Compared to CK , CH4 emission increased by 11% , 1% , 26% and - 4% in treatments of BF , DNP1 , DNP2 and INP within late rice growing season .
Applying nitrogen and phosphate enhanced CH4 emission in turning green period for early and late rice .
No significant difference was observed between the CH4 emissions of five treatments during early and late rice growing season ( p > 0 . 05 ) .
N2O emission was very little during mid-seasonal drainage period .
In contrast , N2O emission peaks were observed in period of alternation of wetting and drying after mid-seasonal drainage in this experiment .
N2O emission was , on average , equivalent to 0 . 18% of the nitrogen applied in double rice growing season .
Statistically , air temperature , soil Eh and soil moisture ( water-filled pore space , WFPS ) at 0-10cm depth significantly affected the fluctuations of the seasonal CH4 flux , but no significant correlationship has been found between N2O flux and related environmental factors .
CH4 was the dominated greenhouse gas in double rice fields which contributed approximately 90% for the integrated global warming potential of CH4 and N2O released during the rice growing season .
Therefore , the mitigation options should focus on how to reduce CH4 emission in local area .
The result indicates that BF is a recommended fertilization method for early rice production , and a optimum fertilization for late season can increase rates of nitrogen and phosphate fertilizers on the basis of BF treatment slightly by considering total global warming potential and grain yield .
The rates of BF treatment were 150-90-90 kg x hm ( -2 ) N-P2O5-K2O for early rice , and 180-90-135 kg x hm ( -2 ) N-P2O5-K2O for late rice , respectively .
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[ Sen. 13, subscore: 2.00 ]: The result indicates that BF is a recommended fertilization method for early rice production , and a optimum fertilization for late season can increase rates of nitrogen and phosphate fertilizers on the basis of BF treatment slightly by considering total global warming potential and grain yield .
[ Sen. 1, subscore: 1.00 ]: Two non-CO2 greenhouse gas emissions ( methane and nitrous oxide ) and related environmental factors were measured within rice growing season under five treatments including non-fertilization ( CK ) , balanced fertilization ( BF ) , decreased nitrogen and phosphate 1 ( DNP1 ) , decreased nitrogen and phosphate 2 ( DNP2 ) and increased nitrogen and phosphate 1 ( INP ) in double rice fields of red clay soil in 2009 , using the method of static chamber-gas chromatograph techniques .
[ Sen. 2, subscore: 1.00 ]: The results showed that the average CH4 emission fluxes for treatments of BF , DNP1 , DNP2 and INP were 4 . 57 , 5 . 42 , 4 . 70 and 4 . 65 mg x ( m2 x h ) ( -1 ) during early rice growing period , which increased by 39% , 49% , 41% and 40% compared with non-fertilizer treatment , respectively .
[ Sen. 4, subscore: 1.00 ]: Compared to CK , CH4 emission increased by 11% , 1% , 26% and - 4% in treatments of BF , DNP1 , DNP2 and INP within late rice growing season .
[ Sen. 14, subscore: 1.00 ]: The rates of BF treatment were 150-90-90 kg x hm ( -2 ) N-P2O5-K2O for early rice , and 180-90-135 kg x hm ( -2 ) N-P2O5-K2O for late rice , respectively .
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Score: 5.00 |
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| Title: The effects of decreasing low-molecular weight hemoglobin components of hemoglobin-based oxygen carriers in swine with hemorrhagic shock .
| | Author: Rice J Philbin N Light R Arnaud F Steinbach T McGwin G Collier S Malkevich N Moon-Massatt P Rentko V Pearce LB Ahlers S McCarron R Handrigan M Freilich D | | Journal: J Trauma Citation: V : 64 P : 1240-57 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18469646 Accession (PMID): 18469646 | | Abstract: BACKGROUND : Some hemoglobin-based oxygen carriers ( HBOCs ) improve outcome in animal models of hemorrhagic shock ( HS ) in comparison with standard asanguinous resuscitation fluids .
Nevertheless , concern about intrinsic vasoactivity , linked in part to low-molecular weight ( MW ) hemoglobin ( Hb ) , has slowed HBOC development .
We assessed the impact of decreasing the low-MW Hb component of bovine HBOC on vasoactivity in severe HS .
METHODS : Anesthetized invasively monitored swine were hemorrhaged 55% blood volume and resuscitated with bovine HBOC containing 31% ( 31 TD [ HBOC-301 ] ) , 2% ( 2 TD [ HBOC-201 ] ) , or 0 . 4% ( 0 . 4 TD ) low-MW Hb .
Pigs received four 10 mL/kg infusions over 60 minutes , hospital arrival was simulated at 75 minutes , organ blood flow ( BF ) was evaluated by microsphere injection , and monitoring was continued for 4 hours followed by complete necrotic evaluation .
RESULTS : There were few differences between 2 TD and 0 . 4 TD .
Thirty-one TD pigs had higher systemic and pulmonary blood pressure ( BP ) , systemic vascular resistance index , and pulmonary artery wedge pressure , compared with 2 TD or 0 . 4 TD ( p < 0 . 01 ) ; however , pigs in all groups had at least mildly elevated BP .
Transcutaneous it issue oxygenation , base excess , and mixed venous oxygen saturation were similar across groups ; lactate and methemoglobin were highest with 0 . 4 TD ( p < 0 . 03 ) .
There were no group differences in BF .
Over time , myocardial BF increased and hepatic BF decreased in all groups ( for 31 TD , p < 0 . 05 ) ; renal BF was unchanged in all groups .
There were no group differences in heart , lung , or liver histopathology , and survival CONCLUSIONS : Although purification from 31% to 2% low-MW Hb content significantly decreased vasoactive responses , further purification to 0 . 4% had no additional clinically measurable effects in severe HS .
If further diminution in HBOC vasoactivity is desired for use in HS , additional technical approaches may be required .
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[ Sen. 10, subscore: 3.00 ]: Over time , myocardial BF increased and hepatic BF decreased in all groups ( for 31 TD , p < 0 . 05 ) ; renal BF was unchanged in all groups .
[ Sen. 5, subscore: 1.00 ]: Pigs received four 10 mL/kg infusions over 60 minutes , hospital arrival was simulated at 75 minutes , organ blood flow ( BF ) was evaluated by microsphere injection , and monitoring was continued for 4 hours followed by complete necrotic evaluation .
[ Sen. 9, subscore: 1.00 ]: There were no group differences in BF .
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Score: 5.00 |
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| Title: Enhancement of growth and nutrient uptake of rapeseed ( Brassica napus L ) by applying mineral nutrients and biofertilizers .
| | Author: Yasari E Azadgoleh MA Mozafari S Alashti MR | | Journal: Pak J Biol Sci Citation: V : 12 P : 127-33 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19579932 Accession (PMID): 19579932 | | Abstract: For investigating the effect of chemical fertilizer as well as biofertilizers on seed yield and quality ie oil , protein and nutrients concentration of rapeseed ( Brassica napus L ) , a split-plot fertilizers application experimental design in 4 replications was carried out during the 2005-2006 growing season , at the Gharakheil Agricultural Research Station in the Mazandaran province of Iran .
Rapeseed was grown as a second crop in rotation after rice .
Biofertilizers treatments were two different levels : control ( no seed inoculation ) and seeds inoculation with a combination of Azotobacter chroococcum and Azosprillum brasilense and Azosprillum lipoferum , as main plot and chemical fertilizers comprised N , P , K and their combinations , NPKS and NPK Zn as sub plots .
The maximum value of seed yield obtained at ( BF+NPK Zn ) 3421 . 2 kg h ( -1 ) corresponding to 244 . 5 pods per plant and maximum concentration of Zn in leaves as well as seeds .
The highest weight of 1000 seeds ( 4 . 45 g ) happened to obtain at ( BF+NPK S ) which coinciding with the maximum K levels in leaves .
The highest number of branches was obtained at ( BF+NPK Zn ) with 4 . 43 branches per plant ie , 46 . 2% increase over the control .
The maximum value of rapeseed oil content 47 . 73% obtained at T16 ( BF+NK ) but maximum protein concentration of seed obtained at T12 ( BF+N ) .
Overall the results indicated that inoculation resulted in increase in seeds yield ( 21 . 17% ) , number of pods per plant ( 16 . 05% ) , number of branches ( 11 . 78% ) , weight of 1000 grain ( 2 . 92% ) , oil content of seeds ( 1 . 73% ) and protein ( 3 . 91% ) but decrease ( -0 . 24% ) in number of seeds per pods comparing to non-Biofertilizers treatments .
Irrespective to the treatments , results showed that application of Biofertilizers coincided with 3 . 86 , 0 . 82 , 2 . 25 , 0 . 75 and 0 . 91% increase in concentrations of N , P , K , S and Zn in the seeds over the non-Biofertilizers treatments .
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[ Sen. 7, subscore: 2.00 ]: The maximum value of rapeseed oil content 47 . 73% obtained at T16 ( BF+NK ) but maximum protein concentration of seed obtained at T12 ( BF+N ) .
[ Sen. 4, subscore: 1.00 ]: The maximum value of seed yield obtained at ( BF+NPK Zn ) 3421 . 2 kg h ( -1 ) corresponding to 244 . 5 pods per plant and maximum concentration of Zn in leaves as well as seeds .
[ Sen. 5, subscore: 1.00 ]: The highest weight of 1000 seeds ( 4 . 45 g ) happened to obtain at ( BF+NPK S ) which coinciding with the maximum K levels in leaves .
[ Sen. 6, subscore: 1.00 ]: The highest number of branches was obtained at ( BF+NPK Zn ) with 4 . 43 branches per plant ie , 46 . 2% increase over the control .
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Score: 5.00 |
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| Title: Additive and synergistic effects of bFGF and hypoxia on leporine meniscus cell-seeded PLLA scaffolds .
| | Author: Gunja NJ Athanasiou KA | | Journal: J it issue Eng Regen Med Citation: V : P : Year: 2009 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub19937913 Accession (PMID): 19937913 | | Abstract: Injuries to avascular regions of menisci do not heal and result in significant discomfort to patients .
Current treatments , such as partial meniscectomy , alleviate these symptoms in the short term but lead to premature osteoarthritis as a result of compromised stability and changes in knee biomechanics .
Thus , it issue engineering of the meniscus may provide an alternative treatment modality to overcome this problem .
In this experiment , a scaffold-based it issue-engineering approach was utilized to regenerate the meniscus .
Meniscus cells were cultured on poly-L-lactic acid scaffolds in normoxic ( approximately 21% oxygen ) or hypoxic ( approximately 2% oxygen ) conditions in the presence or absence of the growth factor , basic fibroblast growth factor ( bFGF ) .
At t = 4 weeks , histological sections of constructs showed presence of collagen and glycosaminoglycan ( GAG ) in all groups .
Immunohistochemical staining showed the presence of collagen I in all groups and collagen II in groups cultured under hypoxic conditions . bFGF in the culture medium significantly increased cell number/construct by 25% , regardless of culture conditions .
For GAG/construct , synergistic increases were observed in constructs cultured in hypoxic conditions and bFGF ( two-fold ) when compared to constructs cultured in normoxic conditions .
Compressive tests showed synergistic increases in the relaxation modulus and coefficient of viscosity and additive increases in the instantaneous modulus for constructs cultured under hypoxic conditions and bFGF , when compared to constructs cultured under normoxic conditions .
Overall , these results demonstrate that bFGF and hypoxia can significantly enhance the ability of meniscus cells to produce GAGs and improve the compressive properties of it issue-engineered meniscus constructs in vitro .
Copyright ( c ) 2009 John Wiley & Sons , Ltd .
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[ Sen. 5, subscore: 1.00 ]: Meniscus cells were cultured on poly-L-lactic acid scaffolds in normoxic ( approximately 21% oxygen ) or hypoxic ( approximately 2% oxygen ) conditions in the presence or absence of the growth factor , basic fibroblast growth factor ( bFGF ) .
[ Sen. 7, subscore: 1.00 ]: Immunohistochemical staining showed the presence of collagen I in all groups and collagen II in groups cultured under hypoxic conditions . bFGF in the culture medium significantly increased cell number/construct by 25% , regardless of culture conditions .
[ Sen. 8, subscore: 1.00 ]: For GAG/construct , synergistic increases were observed in constructs cultured in hypoxic conditions and bFGF ( two-fold ) when compared to constructs cultured in normoxic conditions .
[ Sen. 9, subscore: 1.00 ]: Compressive tests showed synergistic increases in the relaxation modulus and coefficient of viscosity and additive increases in the instantaneous modulus for constructs cultured under hypoxic conditions and bFGF , when compared to constructs cultured under normoxic conditions .
[ Sen. 10, subscore: 1.00 ]: Overall , these results demonstrate that bFGF and hypoxia can significantly enhance the ability of meniscus cells to produce GAGs and improve the compressive properties of it issue-engineered meniscus constructs in vitro .
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