| 11 matches found in 5 documents. Search time: 0.636 seconds. |
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| Title: Evolutionary history of the Asr gene family .
| | Author: Frankel N Carrari F Hasson E Iusem ND .
| | Journal: Gene Citation: V : 378 ( ) P : 74-83 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16822623 Accession (PMID): 16822623 | | Abstract: The Asr gene family is widespread in higher plants .
Most Asr genes are up-regulated under different environmental stress conditions and during fruit ripening .
ASR proteins are localized in the nucleus and their likely function is transcriptional regulation .
In cultivated tomato , we identified a novel fourth family member , named Asr4 , which maps close to its sibling genes Asr1-Asr2-Asr3 and displays an unshared region coding for a domain containing a 13-amino acid repeat .
In this work we were able to expand our previous analysis for Asr2 and investigated the coding regions of the four known Asr paralogous genes in seven tomato species from different geographic locations .
In addition , we performed a phylogenetic analysis on ASR proteins .
The first conclusion drawn from this work is that tomato ASR proteins cluster together in the tree .
This observation can be explained by a scenario of concerted evolution or birth and death of genes .
Secondly , our study showed that Asr1 is highly conserved at both replacement and synonymous sites within the genus Lycopersicon .
ASR1 protein sequence conservation might be associated with its multiple functions in different it issues while the low rate of synonymous substitutions suggests that silent variation in Asr1 is selectively constrained , which is probably related to its high expression levels .
Finally , we found that Asr1 activation under water stress is not conserved between Lycopersicon species . | Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: ASR1 protein sequence conservation might be associated with its multiple functions in different it issues while the low rate of synonymous substitutions suggests that silent variation in Asr1 is selectively constrained , which is probably related to its high expression levels .
[ Sen. 4, subscore: 1.00 ]: In cultivated tomato , we identified a novel fourth family member , named Asr4 , which maps close to its sibling genes Asr1-Asr2-Asr3 and displays an unshared region coding for a domain containing a 13-amino acid repeat .
[ Sen. 9, subscore: 1.00 ]: Secondly , our study showed that Asr1 is highly conserved at both replacement and synonymous sites within the genus Lycopersicon .
[ Sen. 11, subscore: 1.00 ]: Finally , we found that Asr1 activation under water stress is not conserved between Lycopersicon species .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae .
| | Author: Kim IS Kim YS Yoon HS | | Journal: Mol Cells Citation: V : 33 P : 285-93 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22382682 Accession (PMID): 22382682 | | Abstract: Abscisic acid stress ripening ( ASR1 ) protein is a small hydrophilic , low molecular weight , and stress-specific plant protein .
The gene coding region of ASR1 protein , which is induced under high salinity in rice ( Oryza sativa Ilmi ) , was cloned into a yeast expression vector pVTU260 and transformed into yeast cells .
Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide ( H ( 2 ) O ( 2 ) ) , high salinity ( NaCl ) , heat shock , menadione , copper sulfate , sulfuric acid , lactic acid , salicylic acid , and also high concentration of ethanol .
In particular , the expression of metabolic enzymes ( Fba1p , Pgk1p , Eno2p , Tpi1p , and Adh1p ) , antioxidant enzyme ( Ahp1p ) , molecular chaperone ( Ssb1p ) , and pyrimidine biosynthesis-related enzyme ( Ura1p ) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells .
All of these enzymes contribute to an alleviated redox state to H2O2-induced oxidative stress .
In the in vitro assay , the purified ASR1 protein was able to scavenge ROS by converting H ( 2 ) O ( 2 ) to H ( 2 ) O Taken together , these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells .
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[ Sen. 6, subscore: 2.00 ]: In the in vitro assay , the purified ASR1 protein was able to scavenge ROS by converting H ( 2 ) O ( 2 ) to H ( 2 ) O Taken together , these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells .
[ Sen. 1, subscore: 1.00 ]: Abscisic acid stress ripening ( ASR1 ) protein is a small hydrophilic , low molecular weight , and stress-specific plant protein .
[ Sen. 2, subscore: 1.00 ]: The gene coding region of ASR1 protein , which is induced under high salinity in rice ( Oryza sativa Ilmi ) , was cloned into a yeast expression vector pVTU260 and transformed into yeast cells .
[ Sen. 3, subscore: 1.00 ]: Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide ( H ( 2 ) O ( 2 ) ) , high salinity ( NaCl ) , heat shock , menadione , copper sulfate , sulfuric acid , lactic acid , salicylic acid , and also high concentration of ethanol .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp .
ASR1 .
| | Author: Sajidan A Farouk A Greiner R Jungblut P MEler EC Borriss R | | Journal: Appl . Microbiol . Biotechnol . Citation: V : 65 ( 1 ) P : 110-8 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14727093 Accession (PMID): 14727093 | | Abstract: Klebsiella sp . strain ASR1 isolated from an Indonesian rice field is able to hydrolyse myo-inositol hexakis phosphate ( phytate ) .
The phytase protein was purified and characterised as a 42 kDa protein accepting phytate , NADP and sugar phosphates as substrates .
The corresponding gene ( phyK ) was cloned from chromosomal DNA using a combined approach of protein and genome analysis , and expressed in Escherichia coli .
The recombinant enzyme was identified as a 3-phytase yielding myo-inositol monophosphate , Ins ( 2 ) P , as the final product of enzymatic phytate hydrolysis .
Based on its amino acid sequence , PhyK appears to be a member of a hitherto unknown subfamily of histidine acid phytate-degrading enzymes with the active site RHGXRXP and HD sequence motifs , and is different from other general phosphatases and phytases .
Due to its ability to degrade sodium phytate to the mono phosphate ester , the phyK gene product is an interesting candidate for industrial and agricultural applications to make phytate phosphorous available for plant and animal nutrition .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Klebsiella sp . strain ASR1 isolated from an Indonesian rice field is able to hydrolyse myo-inositol hexakis phosphate ( phytate ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp .
ASR1 .
| | Author: Sajidan A Farouk A Greiner R Jungblut P MEler EC Borriss R | | Journal: Appl . Microbiol . Biotechnol . Citation: V : 65 ( 1 ) P : 110-8 Year: 2004 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub14727093 Accession (PMID): 14727093 | | Abstract: Klebsiella sp . strain ASR1 isolated from an Indonesian rice field is able to hydrolyse myo-inositol hexakis phosphate ( phytate ) .
The phytase protein was purified and characterised as a 42 kDa protein accepting phytate , NADP and sugar phosphates as substrates .
The corresponding gene ( phyK ) was cloned from chromosomal DNA using a combined approach of protein and genome analysis , and expressed in Escherichia coli .
The recombinant enzyme was identified as a 3-phytase yielding myo-inositol monophosphate , Ins ( 2 ) P , as the final product of enzymatic phytate hydrolysis .
Based on its amino acid sequence , PhyK appears to be a member of a hitherto unknown subfamily of histidine acid phytate-degrading enzymes with the active site RHGXRXP and HD sequence motifs , and is different from other general phosphatases and phytases .
Due to its ability to degrade sodium phytate to the mono phosphate ester , the phyK gene product is an interesting candidate for industrial and agricultural applications to make phytate phosphorous available for plant and animal nutrition .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: ASR1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae .
| | Author: Kim IS Kim YS Yoon HS | | Journal: Mol Cells Citation: V : 33 P : 285-93 Year: 2012 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub22382682 Accession (PMID): 22382682 | | Abstract: Abscisic acid stress ripening ( ASR1 ) protein is a small hydrophilic , low molecular weight , and stress-specific plant protein .
The gene coding region of ASR1 protein , which is induced under high salinity in rice ( Oryza sativa Ilmi ) , was cloned into a yeast expression vector pVTU260 and transformed into yeast cells .
Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide ( H ( 2 ) O ( 2 ) ) , high salinity ( NaCl ) , heat shock , menadione , copper sulfate , sulfuric acid , lactic acid , salicylic acid , and also high concentration of ethanol .
In particular , the expression of metabolic enzymes ( Fba1p , Pgk1p , Eno2p , Tpi1p , and Adh1p ) , antioxidant enzyme ( Ahp1p ) , molecular chaperone ( Ssb1p ) , and pyrimidine biosynthesis-related enzyme ( Ura1p ) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells .
All of these enzymes contribute to an alleviated redox state to H2O2-induced oxidative stress .
In the in vitro assay , the purified ASR1 protein was able to scavenge ROS by converting H ( 2 ) O ( 2 ) to H ( 2 ) O Taken together , these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
