Score: 2.00 |
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| Title: Molecular cloning , expression and mapping analysis of a novel cytosolic ascorbate peroxidase gene from tomato .
| | Author: Zou LP Li HX Ouyang B Zhang JH Ye ZB .
| | Journal: DNA Seq .
Citation: V : 16 ( 6 ) P : 456-61 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16287625 Accession (PMID): 16287625 | | Abstract: Ascorbate peroxidase ( APX , EC 1 . 11 . 1 . 11 ) plays a major role in H ( 2 ) O ( 2 ) -scavenging in plants and can help to avoid reactive oxygen species ( ROS ) damage .
A new cytosolic APX gene was cloned from tomato ( designated LecAPX2 ) by RACE-PCR .
The full-length cDNA of LecAPX2 contained a complete open reading frame ( ORF ) of 753 bp , which encoding 250 amino acid residues .
Homology analysis of LecAPX2 showed a 94% identity with potato cAPX gene and 92% identity with another tomato cAPX gene ( APX20 ) , the deduced amino acid showed 88% homology with APX20 protein and 75-92% identity with cAPX from other plants such as potato , tobacco , broccoli , spinach , pea , rice , etc LecAPX2 revealed the existence of a haem peroxidase and plant APX family signatures .
Northern blot analysis showed that LecAPX2 was constitutively expressed in root , stem , leaf , flower and fruit of tomato , whereas the expression levels were different .
LecAPX2 was mapped to 6-A using 75 tomato introgression lines ( ILs ) , each containing a single homozygous RFLP-defined chromosome segment from the green-fruited species Lycopersicon pennellii .
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[ Sen. 4, subscore: 2.00 ]: Homology analysis of LecAPX2 showed a 94% identity with potato cAPX gene and 92% identity with another tomato cAPX gene ( APX20 ) , the deduced amino acid showed 88% homology with APX20 protein and 75-92% identity with cAPX from other plants such as potato , tobacco , broccoli , spinach , pea , rice , etc LecAPX2 revealed the existence of a haem peroxidase and plant APX family signatures .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Cytosolic APx knockdown indicates an ambiguous redox responses in rice .
| | Author: Rosa SB Caverzan A Teixeira FK Lazzarotto F Silveira JA Ferreira-Silva SL Abreu-Neto J Margis R Margis-Pinheiro M | | Journal: Phytochemistry Citation: V : 71 P : 548-58 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20129631 Accession (PMID): 20129631 | | Abstract: Ascorbate peroxidases ( APX , EC 1 . 1 . 11 . 1 ) are class I heme-peroxidases , which catalyze the conversion of H ( 2 ) O ( 2 ) into H ( 2 ) O , using ascorbate as a specific electron donor .
Previously , the presence of eight Apx genes was identified in the nuclear genome of rice ( Oryza sativa ) , encoding isoforms that are located in different sub-cellular compartments .
Herein , the generation of rice transgenic plants silenced for either both or each one of the cytosolic Apx1 and Apx2 genes was carried out in order to investigate the importance of cytosolic Apx isoforms on plant development and on plant stress responses .
Transgenic double Apx1/2-silenced plants exhibited normal development , even though these plants showed a global reduction of Apx activity which strongly impacts the whole antioxidant system regulation .
Apx1/2-silenced plants also showed increased H ( 2 ) O ( 2 ) accumulation under control and stress situations and presented higher tolerance to toxic concentration of aluminum when compared to wild type plants .
On the other hand , silencing OsApx1 and OsApx2 genes individually resulted in strong effect on plant development producing semi-dwarf phenotype .
These results suggested that the double silencing of cytosolic OsApx genes induced compensatory antioxidant mechanisms in rice while single knockdown of these genes did not , which resulted in the impairing of normal plant development .
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[ Sen. 3, subscore: 1.00 ]: Herein , the generation of rice transgenic plants silenced for either both or each one of the cytosolic Apx1 and Apx2 genes was carried out in order to investigate the importance of cytosolic Apx isoforms on plant development and on plant stress responses .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
