| 87 matches found in 70 documents. Search time: 0.64 seconds. |
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Score: 11.00 |
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| Title: Novel transgenic rice overexpressing anthocyanidin synthase accumulates a mixture of flavonoids leading to an increased antioxidant potential | | Author: Reddy AM Reddy VS Scheffler BE Wienand U Reddy AR .
| | Journal: Metab . Eng . Citation: V : 9 ( 1 ) P : 95-111 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17157544 Accession (PMID): 17157544 | | Abstract: In addition to their plant-associated functions , flavonoids act as antioxidants against harmful free radicals in animals .
Genetic engineering of food crops for a mix of antioxidant flavonoids is highly beneficial in promoting human health .
Anthocyanidin synthase ( ANS ) is one of the four dioxygenases ( DOX ) of the flavonoid biosynthetic pathway that catalyzes the formation of anthocyanidins from leucoanthocyanidins .
To investigate whether ANS mediates different DOX reactions of the pathway and produces a mix of flavonoids , the rice ANS cDNA was cloned and overexpressed in a rice mutant Nootripathu ( NP ) .
This mutant accumulates proanthocyanidins exclusively in pericarp and absolutely no anthocyanins in any it issue .
In silico sequence analysis revealed that ANS contains a double-stranded beta helix and shows high sequence similarity with other DOXs of the pathway including flavonol synthase , flavonone 3beta-hydroxylase and flavone synthase I Bacterially expressed ANS protein converted dihydroquercetin to quercetin and Pro ( 35S ) : ANS complemented the maize a2 mutant in producing anthocyanins in aleurone , suggesting that ANS functions as a DOX with different flavonoid substrates .
Similarly , transgenic NP plants overexpressing Pro ( MAS ) : ANS channeled the proanthocaynidin precursors to the production of anthocyanins in pericarp .
Transgenics showed approximately ten and four-fold increase in the ANS transcripts and enzyme activity , respectively .
As a result , these plants showed an increased accumulation of a mixture of flavonoids and anthocyanins , with a concomitant decrease in proanthocyanidins , suggesting that ANS may act directly on different flavonoid substrates of DOX reactions .
Thus , overexpression of ANS in a rice mutant resulted in novel transgenic rice with a mixture of flavonoids and an enhanced antioxidant potential | Matching Sentences:
[ Sen. 6, subscore: 4.00 ]: In silico sequence analysis revealed that ANS contains a double-stranded beta helix and shows high sequence similarity with other DOXs of the pathway including flavonol synthase , flavonone 3beta-hydroxylase and flavone synthase I Bacterially expressed ANS protein converted dihydroquercetin to quercetin and Pro ( 35S ) : ANS complemented the maize a2 mutant in producing anthocyanins in aleurone , suggesting that ANS functions as a DOX with different flavonoid substrates .
[ Sen. 4, subscore: 2.00 ]: To investigate whether ANS mediates different DOX reactions of the pathway and produces a mix of flavonoids , the rice ANS cDNA was cloned and overexpressed in a rice mutant Nootripathu ( NP ) .
[ Sen. 3, subscore: 1.00 ]: Anthocyanidin synthase ( ANS ) is one of the four dioxygenases ( DOX ) of the flavonoid biosynthetic pathway that catalyzes the formation of anthocyanidins from leucoanthocyanidins .
[ Sen. 7, subscore: 1.00 ]: Similarly , transgenic NP plants overexpressing Pro ( MAS ) : ANS channeled the proanthocaynidin precursors to the production of anthocyanins in pericarp .
[ Sen. 8, subscore: 1.00 ]: Transgenics showed approximately ten and four-fold increase in the ANS transcripts and enzyme activity , respectively .
[ Sen. 9, subscore: 1.00 ]: As a result , these plants showed an increased accumulation of a mixture of flavonoids and anthocyanins , with a concomitant decrease in proanthocyanidins , suggesting that ANS may act directly on different flavonoid substrates of DOX reactions .
[ Sen. 10, subscore: 1.00 ]: Thus , overexpression of ANS in a rice mutant resulted in novel transgenic rice with a mixture of flavonoids and an enhanced antioxidant potential
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
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| Title: Comparing clinician knowledge and online information regarding Alli ( Orlistat ) .
| | Author: Nelson S Hwang KO Bernstam EV | | Journal: Int J Med Inform Citation: V : 78 P : 772-7 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19716762 Accession (PMID): 19716762 | | Abstract: BACKGROUND : Many consumers join online communities focused on health .
Online forums are a popular medium for the exchange of health information between consumers , so it is important to determine the accuracy and completeness of information posted to online forums .
OBJECTIVE : We compared the accuracy and completeness of information regarding the FDA-approved over-the counter weight-loss drug Alli ( Orlistat ) from forums and from clinicians .
METHODS : We identified Alli-related questions posted on online forums and then posed the questions to 11 primary care providers .
We then compared the clinicians answers to the answers given on the forums .
A panel of blinded experts evaluated the accuracy and completeness of the answers on a scale of 0-4 .
Another panel of blinded experts categorized questions as being best answered based on clinical experience versus review of the literature .
RESULTS : The accuracy and completeness of clinician responses was slightly better than forum responses , but there was no significant difference ( 2 . 3 vs 2 . 1 , p=0 . 5 ) .
Only one forum answer contained information that could potentially cause harm if the advice was followed .
CONCLUSIONS : Forum answers were comparable to clinicians answers with respect to accuracy and completeness , but answers from both sources were unsatisfactory .
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[ Sen. 10, subscore: 3.00 ]: CONCLUSIONS : Forum answers were comparable to clinicians answers with respect to accuracy and completeness , but answers from both sources were unsatisfactory .
[ Sen. 5, subscore: 2.00 ]: We then compared the clinicians answers to the answers given on the forums .
[ Sen. 6, subscore: 1.00 ]: A panel of blinded experts evaluated the accuracy and completeness of the answers on a scale of 0-4 .
[ Sen. 7, subscore: 1.00 ]: Another panel of blinded experts categorized questions as being best answered based on clinical experience versus review of the literature .
[ Sen. 9, subscore: 1.00 ]: Only one forum answer contained information that could potentially cause harm if the advice was followed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Possible mechanism for the inhibition of lectin-erythrocyte interaction in presence of endogenous lectin receptor .
| | Author: Basu PS Datta PK Datta TK .
| | Journal: Biosci . Rep . Citation: V : 16 ( 6 ) P : 453-8 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9062696 Accession (PMID): 9062696 | | Abstract: The presence of hydrophobic sites in the lectin-I molecule was indicated by hydrophobic probes like 1-anilinonapthalene-8-sulfonic acid ( ANS ) , 2-p-toluidinyl napthalene-6-sulfonic acid ( TNS ) .
N-phenyl-1-napthylamine ( NA ) and rose bengal ( RB ) .
This was further confirmed by amino acid modifications in the hydrophobic region of the lectin-I molecule .
The binding of ANS , TNS , NA and RB to lectin-I was affected in the presence of NaCl .
The involvement of hydrophobic interactions in rice-bean lectin-I-endogenous lectin receptor ( ELR ) complex were indicated by alterations in the circular dichroism and fluorescence emission spectra .
The percentage of beta-conformation ( 55-63% ) of lectin-I was decreased by addition of ELR .
ELR on reacting with lectin-I reduced the fluorescence emissions of the hydrophobic probes while fluorescence emission of ANS , TNS , NA and RB were greatly enhanced in presence of lectin-I alone .
N-aceyl-galactosamine did not change the fluorescence emissions of any of the hydrophobic probes in presence or in absence of lectin-I This demonstrates that carbohydrate and hydrophobic sites may be different and non-interacting .
It is proposed that the ELR in reacting with lectin-I , induced conformational changes in the lectin-I molecule and thereby affected its erythroagglutinating activity with human blood group "A" erythrocytes .
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[ Sen. 1, subscore: 1.00 ]: The presence of hydrophobic sites in the lectin-I molecule was indicated by hydrophobic probes like 1-anilinonapthalene-8-sulfonic acid ( ANS ) , 2-p-toluidinyl napthalene-6-sulfonic acid ( TNS ) .
[ Sen. 4, subscore: 1.00 ]: The binding of ANS , TNS , NA and RB to lectin-I was affected in the presence of NaCl .
[ Sen. 7, subscore: 1.00 ]: ELR on reacting with lectin-I reduced the fluorescence emissions of the hydrophobic probes while fluorescence emission of ANS , TNS , NA and RB were greatly enhanced in presence of lectin-I alone .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Rices ansatz for overdamped phi4 kinks at finite temperature .
| | Author: Lythe G Mertens FG .
| | Journal: Citation: V : 67 ( 2 Pt 2 ) P : 027601 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12636870 Accession (PMID): 12636870 | | Abstract: The dynamics of a kink driven by noise is analyzed using the two collective variables of the Rice ansatz : position and width .
Starting from a stochastic partial differential equation , with the phi ( 4 ) potential in the overdamped limit , the pair of stochastic differential equations for the collective variables are derived without approximation other than the ansatz itself .
From the steady state probability density of the kink width , the diffusivity of a kink is calculated .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The dynamics of a kink driven by noise is analyzed using the two collective variables of the Rice ansatz : position and width .
[ Sen. 2, subscore: 1.00 ]: Starting from a stochastic partial differential equation , with the phi ( 4 ) potential in the overdamped limit , the pair of stochastic differential equations for the collective variables are derived without approximation other than the ansatz itself .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Diversity in the Oryza genus .
| | Author: Vaughan DA Morishima H Kadowaki K | | Journal: Curr . Opin . Plant Biol .
Citation: V : 6 ( 2 ) P : 139-46 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12667870 Accession (PMID): 12667870 | | Abstract: The pan-tropical wild relatives of rice grow in a wide variety of habitats : forests , savanna , mountainsides , rivers and lakes .
The completion of the sequencing of the rice nuclear and cytoplasmic genomes affords an opportunity to widen our understanding of the genomes of the genus Oryza .
Research on the Oryza genus has begun to help to answer questions related to domestication , speciation , polyploidy and ecological adaptation that can not be answered by studying rice alone .
The wild relatives of rice have furnished genes for the hybrid rice revolution , and other genes from Oryza species with major impact on rice yields and sustainable rice production are likely to be found .
Care is needed , however , when using wild relatives of rice in experiments and in interpreting the results of these experiments .
Careful checking of species identity , maintenance of herbarium specimens and recording of Genbank accession numbers of material used in experiments should be standard procedure when studying wild relatives of rice . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Research on the Oryza genus has begun to help to answer questions related to domestication , speciation , polyploidy and ecological adaptation that can not be answered by studying rice alone .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
