| 8 matches found in 3 documents. Search time: 1.274 seconds. |
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| Title: The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase .
| | Author: Harris LJ Saparno A Johnston A Prisic S Xu M Allard S Kathiresan A Ouellet T Peters RJ .
| | Journal: Plant Mol . Biol . Citation: V : 59 ( 6 ) P : 881-94 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16307364 Accession (PMID): 16307364 | | Abstract: Using the technique of differential display , a maize transcript was identified whose silk it issue expression is induced in the presence of the ear rot pathogen Fusarium graminearum .
The 3445 nt transcript includes a 727 nt 5 untranslated leader with the potential for extensive secondary structure and represents the maize gene An2 .
An2 encodes a copalyl diphosphate synthase ( CPS ) -like protein with 60% amino acid sequence identity with the maize An1 gene product involved in gibberellin ( GA ) biosynthesis .
Recombinant expression and functional analysis demonstrated that both AN1 and AN2 are ent-copalyl diphosphate ( ent-CPP ) synthases ( ent-CPS ) .
Notably , the presence of an additional ent-CPS gene is consistent with previous reports that maize GA biosynthesis can proceed in the absence of An1 .
In addition , northern blot analysis showed that An2 transcript levels were strongly up-regulated by Fusarium attack , with an increase in silk , husk and ear tip it issues as early as 6 h after inoculation of silk channels with spore suspensions of various Fusarium sp .
Gene expression of a third maize CPS-like gene , Cpsl1 , is not affected by Fusarium infection .
The Fusarium-inducible nature of An2 is also consistent with a previous report that cell-free extracts from maize seedlings produce ent-CPP derived diterpenes in response to Fusarium infection .
However , it is not known whether An2 is involved in defense-related secondary metabolism in addition to GA synthesis . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The 3445 nt transcript includes a 727 nt 5 untranslated leader with the potential for extensive secondary structure and represents the maize gene An2 .
[ Sen. 3, subscore: 1.00 ]: An2 encodes a copalyl diphosphate synthase ( CPS ) -like protein with 60% amino acid sequence identity with the maize An1 gene product involved in gibberellin ( GA ) biosynthesis .
[ Sen. 4, subscore: 1.00 ]: Recombinant expression and functional analysis demonstrated that both AN1 and AN2 are ent-copalyl diphosphate ( ent-CPP ) synthases ( ent-CPS ) .
[ Sen. 6, subscore: 1.00 ]: In addition , northern blot analysis showed that An2 transcript levels were strongly up-regulated by Fusarium attack , with an increase in silk , husk and ear tip it issues as early as 6 h after inoculation of silk channels with spore suspensions of various Fusarium sp .
[ Sen. 8, subscore: 1.00 ]: The Fusarium-inducible nature of An2 is also consistent with a previous report that cell-free extracts from maize seedlings produce ent-CPP derived diterpenes in response to Fusarium infection .
[ Sen. 9, subscore: 1.00 ]: However , it is not known whether An2 is involved in defense-related secondary metabolism in addition to GA synthesis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Identity , regulation , and activity of inducible diterpenoid phytoalexins in maize .
| | Author: Schmelz EA Kaplan F Huffaker A Dafoe NJ Vaughan MM Ni X Rocca JR Alborn HT Teal PE | | Journal: Proc Natl Acad Sci U S A Citation: V : 108 P : 5455-60 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21402917 Accession (PMID): 21402917 | | Abstract: Phytoalexins constitute a broad category of pathogen and insect-inducible biochemicals that locally protect plant it issues .
Because of their agronomic significance , maize and rice have been extensively investigated for their terpenoid-based defenses , which include insect-inducible monoterpene and sesquiterpene volatiles .
Rice also produces a complex array of pathogen-inducible diterpenoid phytoalexins .
Despite the demonstration of fungal-induced ent-kaur-15-ene production in maize over 30 y ago , the identity of functionally analogous maize diterpenoid phytoalexins has remained elusive .
In response to stem attack by the European corn borer ( Ostrinia nubilalis ) and fungi , we observed the induced accumulation of six ent-kaurane-related diterpenoids , collectively termed kauralexins .
Isolation and identification of the predominant Rhizopus microsporus-induced metabolites revealed ent-kaur-19-al-17-oic acid and the unique analog ent-kaur-15-en-19-al-17-oic acid , assigned as kauralexins A3 and B3 , respectively .
Encoding an ent-copalyl diphosphate synthase , fungal-induced An2 transcript accumulation precedes highly localized kauralexin production , which can eventually exceed 100 mug . g ( -1 ) fresh weight .
Pharmacological applications of jasmonic acid and ethylene also synergize the induced accumulation of kauralexins .
Occurring at elevated levels in the scutella of all inbred lines examined , kauralexins appear ubiquitous in maize .
At concentrations as low as 10 mug . mL ( -1 ) , kauralexin B3 significantly inhibited the growth of the opportunistic necrotroph R microsporus and the causal agent of anthracnose stalk rot , Colletotrichum graminicola .
Kauralexins also exhibited significant O nubilalis antifeedant activity .
Our work establishes the presence of diterpenoid defenses in maize and enables a more detailed analysis of their biosynthetic pathways , regulation , and crop defense function .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Encoding an ent-copalyl diphosphate synthase , fungal-induced An2 transcript accumulation precedes highly localized kauralexin production , which can eventually exceed 100 mug . g ( -1 ) fresh weight .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase .
| | Author: Harris LJ Saparno A Johnston A Prisic S Xu M Allard S Kathiresan A Ouellet T Peters RJ .
| | Journal: Plant Mol . Biol . Citation: V : 59 ( 6 ) P : 881-94 Year: 2005 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16307364 Accession (PMID): 16307364 | | Abstract: Using the technique of differential display , a maize transcript was identified whose silk it issue expression is induced in the presence of the ear rot pathogen Fusarium graminearum .
The 3445 nt transcript includes a 727 nt 5 untranslated leader with the potential for extensive secondary structure and represents the maize gene An2 .
An2 encodes a copalyl diphosphate synthase ( CPS ) -like protein with 60% amino acid sequence identity with the maize An1 gene product involved in gibberellin ( GA ) biosynthesis .
Recombinant expression and functional analysis demonstrated that both AN1 and AN2 are ent-copalyl diphosphate ( ent-CPP ) synthases ( ent-CPS ) .
Notably , the presence of an additional ent-CPS gene is consistent with previous reports that maize GA biosynthesis can proceed in the absence of An1 .
In addition , northern blot analysis showed that An2 transcript levels were strongly up-regulated by Fusarium attack , with an increase in silk , husk and ear tip it issues as early as 6 h after inoculation of silk channels with spore suspensions of various Fusarium sp .
Gene expression of a third maize CPS-like gene , Cpsl1 , is not affected by Fusarium infection .
The Fusarium-inducible nature of An2 is also consistent with a previous report that cell-free extracts from maize seedlings produce ent-CPP derived diterpenes in response to Fusarium infection .
However , it is not known whether An2 is involved in defense-related secondary metabolism in addition to GA synthesis . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
