| 12 matches found in 5 documents. Search time: 0.635 seconds. |
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| Title: Molecular and cellular characterizations of a cDNA clone encoding a novel isozyme of aldehyde dehydrogenase from rice .
| | Author: Li Y Nakazono M Tsutsumi N Hirai A | | Journal: Gene Citation: V : 249 ( 1-2 ) P : 67-74 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10831839 Accession (PMID): 10831839 | | Abstract: Aldehyde dehydrogenases ( ALDHs ) are a group of enzymes catalyzing the conversion of aldehydes to the corresponding acids .
In mammals and yeasts , at least two isozymes of ALDH are known to be involved in ethanol metabolism ( cytosolic ALDH1 and mitochondrial ALDH2 ) .
Although mitochondrial ALDH isozymes have previously been identified in several plants , such as maize and tobacco , it is unclear whether cytosolic ALDH isozymes also exist in plants .
In this study , we identified and characterized a cDNA clone encoding aldehyde dehydrogenase ( ALDH1a ) from rice ( Oryza sativa L cv .
Nipponbare ) .
The open reading frame of this clone did not contain a typical mitochondrial targeting signal Analysis of the subcellular localization of ALDH1a using green fluorescent protein ( GFP ) suggested that ALDH1a is a cytosolic enzyme rather than a mitochondrial enzyme .
A genomic Southern hybridization indicated that sequences homologous to the ALDH1a gene are present in at least two regions of the rice genome .
Amplification by RT-PCR showed that ALDH1a is expressed strongly in roots , but not in leaves , of rice seedlings , suggesting that ALDH1a functions in roots . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The open reading frame of this clone did not contain a typical mitochondrial targeting signal Analysis of the subcellular localization of ALDH1a using green fluorescent protein ( GFP ) suggested that ALDH1a is a cytosolic enzyme rather than a mitochondrial enzyme .
[ Sen. 8, subscore: 2.00 ]: Amplification by RT-PCR showed that ALDH1a is expressed strongly in roots , but not in leaves , of rice seedlings , suggesting that ALDH1a functions in roots .
[ Sen. 4, subscore: 1.00 ]: In this study , we identified and characterized a cDNA clone encoding aldehyde dehydrogenase ( ALDH1a ) from rice ( Oryza sativa L cv .
[ Sen. 7, subscore: 1.00 ]: A genomic Southern hybridization indicated that sequences homologous to the ALDH1a gene are present in at least two regions of the rice genome .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Overexpression of stem cell associated ALDH1A1 , a target of the leukemogenic transcription factor TLX1/HOX11 , inhibits lymphopoiesis and promotes myelopoiesis in murine hematopoietic progenitors .
| | Author: Rice KL Izon DJ Ford J Boodhoo A Kees UR Greene WK | | Journal: Leuk Res Citation: V : 32 P : 873-83 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18082256 Accession (PMID): 18082256 | | Abstract: TLX1/HOX11 is an oncogenic transcription factor in human T-cell leukemia , however , the molecular basis for its transforming activity has remained elusive .
The ALDH1A1 gene , whose product participates in retinoic acid synthesis , was previously identified as a TLX1-responsive gene .
Here , we confirm regulation of ALDH1A1 transcription by TLX1 and show that ALDH1A1 can profoundly perturb murine hematopoiesis by promoting myeloid differentiation at the expense of lymphopoiesis .
Together , these data demonstrate that ALDH1A1 plays a key role in normal hematopoiesis , and confirm ALDH1A1 as a TLX1 transcriptional target that may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth .
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[ Sen. 3, subscore: 2.00 ]: Here , we confirm regulation of ALDH1A1 transcription by TLX1 and show that ALDH1A1 can profoundly perturb murine hematopoiesis by promoting myeloid differentiation at the expense of lymphopoiesis .
[ Sen. 4, subscore: 2.00 ]: Together , these data demonstrate that ALDH1A1 plays a key role in normal hematopoiesis , and confirm ALDH1A1 as a TLX1 transcriptional target that may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth .
[ Sen. 2, subscore: 1.00 ]: The ALDH1A1 gene , whose product participates in retinoic acid synthesis , was previously identified as a TLX1-responsive gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Transcriptional regulation of FHL1 by TLX1/HOX11 is dosage , cell-type and promoter context-dependent .
| | Author: Rice KL Kees UR Greene WK | | Journal: Biochem Biophys Res Commun Citation: V : 367 P : 707-13 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18073142 Accession (PMID): 18073142 | | Abstract: TLX1/HOX11 encodes an NK-like homeodomain transcription factor that is both normally required for embryonic development and aberrantly expressed in T-cell acute lymphoblastic leukemia .
Previous studies have shown that TLX1 can regulate target genes including ALDH1A1 and FHL1 .
However , whereas ALDH1A1 is consistently regulated by TLX1 , endogenous FHL1 is only induced in a proportion of fibroblast or T-cell clones stably expressing TLX1 .
Here , we provide an explanation for these findings by demonstrating that the induction of FHL1 , but not ALDH1A1 , requires a high level of TLX1 expression in NIH 3T3 cells .
In luciferase reporter assays , TLX1-mediated repression rather than activation of the FHL1 gene promoter and the magnitude of this effect was strongly influenced by the cellular background .
Together , these results characterize TLX1 as a dual function regulator whose activity in respect to FHL1 is critically dependent upon its cellular concentration , as well as cell type and promoter context .
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[ Sen. 2, subscore: 1.00 ]: Previous studies have shown that TLX1 can regulate target genes including ALDH1A1 and FHL1 .
[ Sen. 3, subscore: 1.00 ]: However , whereas ALDH1A1 is consistently regulated by TLX1 , endogenous FHL1 is only induced in a proportion of fibroblast or T-cell clones stably expressing TLX1 .
[ Sen. 4, subscore: 1.00 ]: Here , we provide an explanation for these findings by demonstrating that the induction of FHL1 , but not ALDH1A1 , requires a high level of TLX1 expression in NIH 3T3 cells .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Associations and interactions between SNPs in the alcohol metabolizing genes and alcoholism phenotypes in European Americans .
| | Author: Sherva R Rice JP Neuman RJ Rochberg N Saccone NL Bierut LJ | | Journal: Alcohol Clin Exp Res Citation: V : 33 P : 848-57 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19298322 Accession (PMID): 19298322 | | Abstract: BACKGROUND : Alcohol dependence is a major cause of morbidity and mortality worldwide and has a strong familial component .
Several linkage and association studies have identified chromosomal regions and/or genes that affect alcohol consumption , notably in genes involved in the 2-stage pathway of alcohol metabolism .
METHODS : Here , we use multiple regression models to test for associations and interactions between 2 alcohol-related phenotypes and SNPs in 17 genes involved in alcohol metabolism in a sample of 1 , 588 European American subjects .
RESULTS : The strongest evidence for association after correcting for multiple testing was between rs1229984 , a nonsynonymous coding SNP in ADH1B , and DSM-IV symptom count ( p = 0 . 0003 ) .
This SNP was also associated with maximum number of drinks in 24 hours ( p = 0 . 0004 ) .
Each minor allele at this SNP predicts 45% fewer DSM-IV symptoms and 18% fewer max drinks .
Another SNP in a splice site in ALDH1A1 ( rs8187974 ) showed evidence for association with both phenotypes as well ( p = 0 . 02 and 0 . 004 , respectively ) , but neither association was significant after accounting for multiple testing .
Minor alleles at this SNP predict greater alcohol consumption .
In addition , pairwise interactions were observed between SNPs in several genes ( p = 0 . 00002 ) .
CONCLUSIONS : We replicated the large effect of rs1229984 on alcohol behavior , and although not common ( MAF = 4% ) , this polymorphism may be highly relevant from a public health perspective in European Americans .
Another SNP , rs8187974 , may also affect alcohol behavior but requires replication .
Also , interactions between polymorphisms in genes involved in alcohol metabolism are likely determinants of the parameters that ultimately affect alcohol consumption .
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[ Sen. 7, subscore: 1.00 ]: Another SNP in a splice site in ALDH1A1 ( rs8187974 ) showed evidence for association with both phenotypes as well ( p = 0 . 02 and 0 . 004 , respectively ) , but neither association was significant after accounting for multiple testing .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Overexpression of stem cell associated ALDH1A1 , a target of the leukemogenic transcription factor TLX1/HOX11 , inhibits lymphopoiesis and promotes myelopoiesis in murine hematopoietic progenitors .
| | Author: Rice KL Izon DJ Ford J Boodhoo A Kees UR Greene WK | | Journal: Leuk Res Citation: V : 32 P : 873-83 Year: 2008 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18082256 Accession (PMID): 18082256 | | Abstract: TLX1/HOX11 is an oncogenic transcription factor in human T-cell leukemia , however , the molecular basis for its transforming activity has remained elusive .
The ALDH1A1 gene , whose product participates in retinoic acid synthesis , was previously identified as a TLX1-responsive gene .
Here , we confirm regulation of ALDH1A1 transcription by TLX1 and show that ALDH1A1 can profoundly perturb murine hematopoiesis by promoting myeloid differentiation at the expense of lymphopoiesis .
Together , these data demonstrate that ALDH1A1 plays a key role in normal hematopoiesis , and confirm ALDH1A1 as a TLX1 transcriptional target that may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Overexpression of stem cell associated ALDH1A1 , a target of the leukemogenic transcription factor TLX1/HOX11 , inhibits lymphopoiesis and promotes myelopoiesis in murine hematopoietic progenitors .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
