Score: 2.00 |
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| Title: On-line preconcentration and separation of Co , Ni and Cd via capillary microextraction on ordered mesoporous alumina coating and determination by inductively plasma mass spectrometry ( ICP-MS ) .
| | Author: Hu W Hu B Jiang Z | | Journal: Anal Chim Acta Citation: V : 572 P : 55-62 Year: 2006 Type: PubMed-not-MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17723461 Accession (PMID): 17723461 | | Abstract: In this paper , an ordered mesoporous alumina coating was prepared and applied to capillary microextraction ( CME ) of trace Co , Ni and Cd for the first time .
The coated capillary was used for on-line coupling CME with inductively plasma mass spectrometry ( ICP-MS ) for the determination of trace metals of Co , Ni and Cd .
The porous structure of Al2O3 coating was examined by SEM and TEM .
The effects of the extraction parameters including pH , sample flow rate and volume , elution solution and interfering ions on the recovery of analytes have been investigated and optimized .
Under the optimized conditions , the limits of detection were 0 . 33 , 1 . 5 and 1 . 4 ng L ( -1 ) for Co , Ni and Cd , respectively , with a preconcentration factor of 10 times .
The precisions for all investigated elements were 2 . 7 , 4 . 1 and 2 . 5% ( c=0 . 05 ng L ( -1 ) , n=7 ) , for Co , Ni and Cd , respectively , and the sample frequency was 8 h ( -1 ) . The proposed method was successfully applied for the analysis of trace metals in water , rice and urine samples with the recovery of 94-105% .
In order to validate the proposed method , two certified reference materials of GBW 0913 human urine and NIES No10-b rice flour were analyzed , and the determination values are in good agreement with the certified values .
The ordered mesoporous Al2O3 coated capillary can be used more than 20 times without decreasing the extraction efficiency .
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[ Sen. 3, subscore: 1.00 ]: The porous structure of Al2O3 coating was examined by SEM and TEM .
[ Sen. 8, subscore: 1.00 ]: The ordered mesoporous Al2O3 coated capillary can be used more than 20 times without decreasing the extraction efficiency .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: A novel endosperm transfer cell-containing region-specific gene and its promoter in rice .
| | Author: Kuwano M Masumura T Yoshida KT | | Journal: Plant Mol Biol Citation: V : 76 P : 47-56 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21409497 Accession (PMID): 21409497 | | Abstract: The endosperm of cereal grains is an important resource for both food and feed .
It contains three major types of it issue : starchy endosperm , the aleurone layer , and transfer cells .
To improve grain quality and quantity using molecular methods , control of transgene expression directed by distinct temporal and spatial promoter activity is necessary .
To identify aleurone layer-specific and/or transfer cell-specific promoters in rice , microarray analyses were performed , comparing the aleurone layer containing transfer cells and the other reproductive and vegetative it issues .
After confirmation by RT-PCR analysis , we identified two putative aleurone layer and/or transfer cell-specific genes , AL1 and AL2 .
The promoter regions of these genes and beta-glucuronidase ( GUS ) fusion constructs were stably transformed into rice .
The GUS expression patterns indicated that the AL1 promoter was active exclusively in the dorsal aleurone layer adjacent to the main vascular bundle .
In rice , transfer cells are differentiated in this region .
Therefore , the promoter of the AL1 gene exhibits transfer cell-containing region-specific activity .
The AL1 gene encodes a putative anthranilate N-hydroxycinnamoyl/benzoyltransferase .
The promoter of this gene will be useful for enhancing uptake of nutrients from the mother cells and protecting filial seeds from pathogen attack .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: After confirmation by RT-PCR analysis , we identified two putative aleurone layer and/or transfer cell-specific genes , AL1 and AL2 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
