| 66 matches found in 30 documents. Search time: 0.398 seconds. |
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Score: 9.00 |
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| Title: Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene .
| | Author: Wang Y Zhang W Cao J McElroy D Wu R | | Journal: Mol . Cell . Biol . Citation: V : 12 ( 8 ) P : 3399-406 Year: 1992 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1630454 Accession (PMID): 1630454 | | Abstract: The promoter of the constitutively expressed rice ( Oryza sativa ) actin 1 gene ( Act1 ) is highly active in transformed rice plants ( W Zhang , D McElroy , and R Wu , Plant Cell 3 : 1150-1160 , 1991 ) .
A region 834 bp upstream of the Act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts ( D McElroy , W Zhang , J Cao , and R Wu , Plant Cell 2 : 163-171 , 1990 ) .
We have constructed a series of Act1 promoter deletions fused to a bacterial beta-glucuronidase reporter sequence ( Gus ) .
Transient expression assays in transformed rice protoplasts , as well as transformed maize cells and it issues , identified two distinct cis-acting regulatory elements in the Act1 promoter .
A 38-bp poly ( dA-dT ) region was found to be a positive regulator of Act1 promoter activity .
Deletion of the poly ( dA-dT ) element lowered Gus expression by at least threefold compared with expression produced by the full-length Act1 promoter .
By gel retardation and footprinting , we identified a ubiquitous rice protein which specifically recognizes this poly ( dA-dT ) element in the constitutively active Act1 promoter .
A CCCAA pentamer repeat-containing region was found to be a negative regulator of the Act1 promoter in transformed rice protoplasts .
Transient expression assays in different maize cells and it issues with use of the Act1 deletion constructs suggested that the CCCAA pentamer repeat region functions in a complex it issue-specific manner .
A CCCAA-binding protein was detected only in root extracts . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The promoter of the constitutively expressed rice ( Oryza sativa ) actin 1 gene ( Act1 ) is highly active in transformed rice plants ( W Zhang , D McElroy , and R Wu , Plant Cell 3 : 1150-1160 , 1991 ) .
[ Sen. 2, subscore: 1.00 ]: A region 834 bp upstream of the Act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts ( D McElroy , W Zhang , J Cao , and R Wu , Plant Cell 2 : 163-171 , 1990 ) .
[ Sen. 3, subscore: 1.00 ]: We have constructed a series of Act1 promoter deletions fused to a bacterial beta-glucuronidase reporter sequence ( Gus ) .
[ Sen. 4, subscore: 1.00 ]: Transient expression assays in transformed rice protoplasts , as well as transformed maize cells and it issues , identified two distinct cis-acting regulatory elements in the Act1 promoter .
[ Sen. 5, subscore: 1.00 ]: A 38-bp poly ( dA-dT ) region was found to be a positive regulator of Act1 promoter activity .
[ Sen. 6, subscore: 1.00 ]: Deletion of the poly ( dA-dT ) element lowered Gus expression by at least threefold compared with expression produced by the full-length Act1 promoter .
[ Sen. 7, subscore: 1.00 ]: By gel retardation and footprinting , we identified a ubiquitous rice protein which specifically recognizes this poly ( dA-dT ) element in the constitutively active Act1 promoter .
[ Sen. 8, subscore: 1.00 ]: A CCCAA pentamer repeat-containing region was found to be a negative regulator of the Act1 promoter in transformed rice protoplasts .
[ Sen. 9, subscore: 1.00 ]: Transient expression assays in different maize cells and it issues with use of the Act1 deletion constructs suggested that the CCCAA pentamer repeat region functions in a complex it issue-specific manner .
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Score: 9.00 |
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| Title: Construction of expression vectors based on the rice actin 1 ( Act1 ) 5 region for use in monocot transformation .
| | Author: McElroy D Blowers AD Jenes B Wu R | | Journal: Mol . Gen . Genet . Citation: V : 231 ( 1 ) P : 150-60 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1753941 Accession (PMID): 1753941 | | Abstract: It has been previously reported that the 5 region of the rice actin 1 gene ( Act1 ) promoted high-level expression of a beta-glucuronidase reporter gene ( Gus ) in transformed rice cells .
In this paper we describe the construction of Act1-based expression vectors for use in monocot transformation .
As part of the development of these vectors , we have evaluated the influence of the Act1 first intron , the Act1-Gus junction-encoded N-terminal amino acids , and the sequence context surrounding the Act1 and Gus translation initiation site on Act1-Gus gene expression in rice and maize cells .
We have found that addition of Act1 intron 1 to the transcription unit of a Gus reporter gene under control of the cauliflower mosaic virus ( CaMV ) 35S promoter stimulated GUS activity more than 10-fold in transformed rice cells .
Optimization of the sequence context around the Gus translation initiation site resulted in a 4-fold stimulation of Gus expression in transformed rice cells .
By utilizing both the Act1 intron 1 and optimized Gus translation initiation site , a 40-fold stimulation in Gus expression from the CaMV 35S promoter has been achieved in transformed rice cells ; very similar results were obtained in transformed maize cells .
Taken together these results suggest that the Act1-based expression vectors described here should promote the expression of foreign genes in most , if not all , transformed monocot cells to levels that have not previously been attainable with alternative expression vectors . | Matching Sentences:
[ Sen. 3, subscore: 4.00 ]: As part of the development of these vectors , we have evaluated the influence of the Act1 first intron , the Act1-Gus junction-encoded N-terminal amino acids , and the sequence context surrounding the Act1 and Gus translation initiation site on Act1-Gus gene expression in rice and maize cells .
[ Sen. 1, subscore: 1.00 ]: It has been previously reported that the 5 region of the rice actin 1 gene ( Act1 ) promoted high-level expression of a beta-glucuronidase reporter gene ( Gus ) in transformed rice cells .
[ Sen. 2, subscore: 1.00 ]: In this paper we describe the construction of Act1-based expression vectors for use in monocot transformation .
[ Sen. 4, subscore: 1.00 ]: We have found that addition of Act1 intron 1 to the transcription unit of a Gus reporter gene under control of the cauliflower mosaic virus ( CaMV ) 35S promoter stimulated GUS activity more than 10-fold in transformed rice cells .
[ Sen. 6, subscore: 1.00 ]: By utilizing both the Act1 intron 1 and optimized Gus translation initiation site , a 40-fold stimulation in Gus expression from the CaMV 35S promoter has been achieved in transformed rice cells ; very similar results were obtained in transformed maize cells .
[ Sen. 7, subscore: 1.00 ]: Taken together these results suggest that the Act1-based expression vectors described here should promote the expression of foreign genes in most , if not all , transformed monocot cells to levels that have not previously been attainable with alternative expression vectors .
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Score: 6.00 |
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| Title: Isolation of an efficient actin promoter for use in rice transformation .
| | Author: McElroy D Zhang W Cao J Wu R | | Journal: Plant Cell Citation: V : 2 ( 2 ) P : 163-71 Year: 1990 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub2136633 Accession (PMID): 2136633 | | Abstract: We have characterized the 5 region of the rice actin 1 gene ( Act1 ) and show that it is an efficient promoter for regulating the constitutive expression of a foreign gene in transgenic rice .
By constructing plasmids with 5 regions from the rice Act1 gene fused to the coding sequence of a gene encoding bacterial beta-glucuronidase , we demonstrate that a region 1 . 3 kilobases upstream of the Act1 translation initiation codon contains all of the 5-regulatory elements necessary for high-level beta-glucuronidase ( GUS ) expression in transient assays of transformed rice protoplasts .
The rice Act1 primary transcript has a noncoding exon separated by a 5 intron from the first coding exon .
Fusions that lack this Act1 intron showed no detectable GUS activity in transient assays of transformed rice protoplasts .
Deletion analysis of the Act1 5 intron suggests that the intron-mediated stimulation of GUS expression is associated , in part , with an in vivo requirement for efficient intron splicing . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: By constructing plasmids with 5 regions from the rice Act1 gene fused to the coding sequence of a gene encoding bacterial beta-glucuronidase , we demonstrate that a region 1 . 3 kilobases upstream of the Act1 translation initiation codon contains all of the 5-regulatory elements necessary for high-level beta-glucuronidase ( GUS ) expression in transient assays of transformed rice protoplasts .
[ Sen. 1, subscore: 1.00 ]: We have characterized the 5 region of the rice actin 1 gene ( Act1 ) and show that it is an efficient promoter for regulating the constitutive expression of a foreign gene in transgenic rice .
[ Sen. 3, subscore: 1.00 ]: The rice Act1 primary transcript has a noncoding exon separated by a 5 intron from the first coding exon .
[ Sen. 4, subscore: 1.00 ]: Fusions that lack this Act1 intron showed no detectable GUS activity in transient assays of transformed rice protoplasts .
[ Sen. 5, subscore: 1.00 ]: Deletion analysis of the Act1 5 intron suggests that the intron-mediated stimulation of GUS expression is associated , in part , with an in vivo requirement for efficient intron splicing .
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Score: 5.00 |
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| Title: Multiple conserved 5 elements are required for high-level pollen expression of the Arabidopsis reproductive actin ACT1 .
| | Author: Vitale A Wu RJ Cheng Z Meagher RB .
| | Journal: Plant Mol . Biol . Citation: V : 52 ( 6 ) P : 1135-51 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14682614 Accession (PMID): 14682614 | | Abstract: The Arabidopsis thaliana actin gene family comprises eight genes , which are divided into two ancient classes , vegetative and reproductive .
We dissected various 5 elements and the conserved expression pattern of the reproductive actin gene ACT1 , which is the most strongly expressed pollen actin gene .
A basal construct containing only 310 bp of sequence upstream of the major transcriptional start site showed essentially full promoter activity in pollen and ovules .
Further truncations of the 5-flanking region and two different 10 bp replacements within a 55 bp conserved domain each caused a several-fold reduction in mature pollen expression .
Intron L , located in the 5-untranslated region ( 5-UTR ) was also required for high-level expression of pollen and organ primordia , and it had the properties of an enhancer .
Pollen expression was not preserved when intron L was precisely replaced by intron L2 from the vegetatively expressed actin gene ACT2 .
ACT1 reporter gene constructs were strongly expressed in both pollen and ovules of tobacco and in the pollen of rice .
Promoter-reporter fusions of the most distantly related Arabidopsis reproductive actin gene ACT1 showed strong expression in tobacco pollen and ovules indistinguishable from that directed by ACT1 .
Thus , multiple conserved cis-sequence elements within the 5-flanking region and 5-UTR of ACT1 direct high levels of reproductive it issue-specific expression . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Promoter-reporter fusions of the most distantly related Arabidopsis reproductive actin gene ACT1 showed strong expression in tobacco pollen and ovules indistinguishable from that directed by ACT1 .
[ Sen. 2, subscore: 1.00 ]: We dissected various 5 elements and the conserved expression pattern of the reproductive actin gene ACT1 , which is the most strongly expressed pollen actin gene .
[ Sen. 7, subscore: 1.00 ]: ACT1 reporter gene constructs were strongly expressed in both pollen and ovules of tobacco and in the pollen of rice .
[ Sen. 9, subscore: 1.00 ]: Thus , multiple conserved cis-sequence elements within the 5-flanking region and 5-UTR of ACT1 direct high levels of reproductive it issue-specific expression .
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Score: 5.00 |
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| Title: Analysis of rice Act1 5 region activity in transgenic rice plants .
| | Author: Zhang W McElroy D Wu R | | Journal: Plant Cell Citation: V : 3 ( 11 ) P : 1155-65 Year: 1991 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1821763 Accession (PMID): 1821763 | | Abstract: The 5 region of the rice actin 1 gene ( Act1 ) has been developed as an efficient regulator of foreign gene expression in transgenic rice plants .
To determine the pattern and level of rice Act1 5 region activity , transgenic rice plants containing the Act1 5 region fused to a bacterial beta-glucuronidase ( Gus ) coding sequence were generated .
Two independent clonal lines of transgenic rice plants were analyzed in detail .
Quantitative analysis showed that it issue from these transgenic rice plants have a level of GUS protein that represents as much as 3% of total soluble protein .
We were able to demonstrate that Act1-Gus gene expression is constitutive throughout the sporophytic and gametophytic it issues of these transgenic rice plants .
Plants from one transgenic line were analyzed for the segregation of GUS activity in pollen by in situ histochemical staining , and the inheritance and stability of Act1-Gus expression were assayed in subsequently derived progeny plants . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: To determine the pattern and level of rice Act1 5 region activity , transgenic rice plants containing the Act1 5 region fused to a bacterial beta-glucuronidase ( Gus ) coding sequence were generated .
[ Sen. 1, subscore: 1.00 ]: The 5 region of the rice actin 1 gene ( Act1 ) has been developed as an efficient regulator of foreign gene expression in transgenic rice plants .
[ Sen. 5, subscore: 1.00 ]: We were able to demonstrate that Act1-Gus gene expression is constitutive throughout the sporophytic and gametophytic it issues of these transgenic rice plants .
[ Sen. 6, subscore: 1.00 ]: Plants from one transgenic line were analyzed for the segregation of GUS activity in pollen by in situ histochemical staining , and the inheritance and stability of Act1-Gus expression were assayed in subsequently derived progeny plants .
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