Score: 6.00 |
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| Title: ACT domain repeat protein 7 , ACR7 , interacts with a chaperone HSP18 . 0-CII in rice nuclei .
| | Author: Hayakawa T Kudo T Ito T Takahashi N Yamaya T | | Journal: Plant Cell Physiol .
Citation: V : 47 ( 7 ) P : 891-904 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16720649 Accession (PMID): 16720649 | | Abstract: The regulatory ACT domains serve as amino acid-binding sites in some amino acid metabolic enzymes and transcriptional regulators in bacteria .
To elucidate the molecular roles of the glutamine ( Gln ) -sensing system in nitrogen ( N ) metabolism in plants , we isolated six genes encoding ACT domain repeat proteins ( ACR1 , and ACR5-ACR9 ) from rice ( Oryza sativa L ) using genomic information on the primary structure composed of four copies of the domain homologous to those of bacterial Gln sensor GLND .
Since expression of ACR7 was the most abundant of the six ACR orthologous genes , we focused on this ACR in the current study .
Gene products of ACR7 were most abundant in young developing leaf blades of rice , and ACR7 protein is specifically localized in the nucleus of the parenchyma cells of phloem and xylem in the vascular bundles .
A yeast two-hybrid screen identified a small heat stress protein ( HSP18 . 0-CII ) as a protein interacting with ACR7 .
Transient expression analysis of HSP18 . 0-CII : sGFP in cultured rice cells , followed by co-immunoprecipitation , suggests that the nuclear ACR7 indeed interacted with nucleocytoplasmic HSP18 . 0-CII in vivo .
The potential ability of nuclear protein ACR7 to bind Gln and the possibility of the protein acting as a Gln sensor in rice leaves is discussed . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Gene products of ACR7 were most abundant in young developing leaf blades of rice , and ACR7 protein is specifically localized in the nucleus of the parenchyma cells of phloem and xylem in the vascular bundles .
[ Sen. 3, subscore: 1.00 ]: Since expression of ACR7 was the most abundant of the six ACR orthologous genes , we focused on this ACR in the current study .
[ Sen. 5, subscore: 1.00 ]: A yeast two-hybrid screen identified a small heat stress protein ( HSP18 . 0-CII ) as a protein interacting with ACR7 .
[ Sen. 6, subscore: 1.00 ]: Transient expression analysis of HSP18 . 0-CII : sGFP in cultured rice cells , followed by co-immunoprecipitation , suggests that the nuclear ACR7 indeed interacted with nucleocytoplasmic HSP18 . 0-CII in vivo .
[ Sen. 7, subscore: 1.00 ]: The potential ability of nuclear protein ACR7 to bind Gln and the possibility of the protein acting as a Gln sensor in rice leaves is discussed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: ACT domain repeat protein 7 , ACR7 , interacts with a chaperone HSP18 . 0-CII in rice nuclei .
| | Author: Hayakawa T Kudo T Ito T Takahashi N Yamaya T | | Journal: Plant Cell Physiol .
Citation: V : 47 ( 7 ) P : 891-904 Year: 2006 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16720649 Accession (PMID): 16720649 | | Abstract: The regulatory ACT domains serve as amino acid-binding sites in some amino acid metabolic enzymes and transcriptional regulators in bacteria .
To elucidate the molecular roles of the glutamine ( Gln ) -sensing system in nitrogen ( N ) metabolism in plants , we isolated six genes encoding ACT domain repeat proteins ( ACR1 , and ACR5-ACR9 ) from rice ( Oryza sativa L ) using genomic information on the primary structure composed of four copies of the domain homologous to those of bacterial Gln sensor GLND .
Since expression of ACR7 was the most abundant of the six ACR orthologous genes , we focused on this ACR in the current study .
Gene products of ACR7 were most abundant in young developing leaf blades of rice , and ACR7 protein is specifically localized in the nucleus of the parenchyma cells of phloem and xylem in the vascular bundles .
A yeast two-hybrid screen identified a small heat stress protein ( HSP18 . 0-CII ) as a protein interacting with ACR7 .
Transient expression analysis of HSP18 . 0-CII : sGFP in cultured rice cells , followed by co-immunoprecipitation , suggests that the nuclear ACR7 indeed interacted with nucleocytoplasmic HSP18 . 0-CII in vivo .
The potential ability of nuclear protein ACR7 to bind Gln and the possibility of the protein acting as a Gln sensor in rice leaves is discussed . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: ACT domain repeat protein 7 , ACR7 , interacts with a chaperone HSP18 . 0-CII in rice nuclei .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
