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Enter keyword(s) and/or category/ies. Selecting categories for a query makes a search more specific. For example, you can retrieve sentences that contain the word HSN and a
Oryza sativa
gene name by typing the keyword 'SPW1' and choosing the category 'gene (Oryza sativa)'. A category hit occurs when a particular word or phrase in the sentence is defined as a member of a particular category. Categories will be concatenated by a Boolean 'and' operation to other categories and keyword(s) if present. To search for terms in categories, click on the Categories/Ontology link above.
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Score:
6.00
Title:
White-core endosperm floury endosperm-4 in rice is generated by knockout mutations in the C-type pyruvate orthophosphate dikinase gene ( OsPPDKB ) .
Author:
Kang HG Park S Matsuoka M An G
Journal:
Plant J
Citation:
V : 42 ( 6 ) P : 901-11
Year:
2005
Type:
ARTICLE
Literature:
oryza
Field:
abstract
Doc ID:
pub15941402
Accession (PMID):
15941402
Abstract:
We have isolated a floury endosperm-4 (
flo4
) rice mutant with a floury-white endosperm but a normal outer portion . Scanning electron microscopic analysis revealed that this abnormal endosperm consisted of loosely packed starch granules . The mutant phenotype was generated by T-DNA insertion into the fifth intron of the OsPPDKB gene encoding pyruvate orthophosphate dikinase ( PPDK ) . Plants containing
flo4
-1 produced no OsPPDKB transcript or the OsPPDKB protein in their developing kernels and leaves . We obtained two additional alleles ,
flo4
-2 and
flo4
-3 , that also showed the same white-core endosperm phenotype . The
flo4
kernels weighed about 6% less than wild-type ones . Starch contents in both kernel types were similar , but the total protein content was slightly higher in the mutant kernels . Moreover , lipid contents were significantly increased in the
flo4
kernels . Expression analyses demonstrated that the cytosolic mRNA of OsPPDKB was induced in the reproductive organs after pollination , and greatly increased until about 10 days after fertilization . This mRNA was localized mainly in the endosperm , aleurone , and scutellum of the developing kernel . Our results suggest that cytosolic PPDK functions in rice to modulate carbon metabolism during grain filling .
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