| 12 matches found in 7 documents. Search time: 0.026 seconds. |
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| Title: Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus , Magnaporthe grisea .
| | Author: Kim ST Kim SG Hwang DH Kang SY Kim HJ Lee BH Lee JJ Kang KY .
| | Journal: Proteomics Citation: V : 4 ( 11 ) P : 3569-78 Year: 2004 | | Literature: oryza Field: abstract Doc ID: pub15478215 Accession (PMID): 15478215 | | Abstract: Proteomic approaches using two-dimensional gel electrophoresis ( 2-DE ) were adopted to identify proteins from rice leaf that are differentially expressed in response to the rice blast fungus , Magnaporthe grisea .
Microscopic observation of inoculated leaf with M grisea revealed that callose deposition and hypersensitive response was clearly visible in incompatible interactions but excessive invading hypha with branches were evident in compatible interactions .
Proteins were extracted from leaves 24 , 48 , and 72 hours after rice blast fungus inoculation .
Eight proteins resolved on the 2-DE gels were induced or increased in the inoculated leaf .
Matrix-assisted laser desorption/ionization-time of flight analysis of these differentially displayed proteins showed them to be two receptor-like protein kinases ( RLK ) , two beta-1 . 3-glucanases ( Glu1 , Glu2 ) , thaumatin-like protein ( TLP ) , peroxidase ( POX 22 . 3 ) , probenazole-inducible protein ( PBZ1 ) , and rice pathogenesis-related 10 ( OsPR-10 ) .
Of these proteins , RLK , TLP , PBZ , and OsPR-10 proteins were induced more in the incompatible interactions than in compatible ones .
A phytohormone , jasmonic acid also induced all eight proteins in leaves .
To confirm whether the expression profile is equal to the 2-DE data , seven cDNA clones were used as probes in Northern hybridization experiments using total RNA from leaf it issues inoculated with incompatible and compatible rice blast fungal races .
The genes encoding POX22 . 3 , Glu1 , Glu2 , TLP , OsRLK , PBZ1 , and OsPR-10 were activated in inoculated leaves , with TLP , OsRLK , PBZ1 , and OsPR-10 being expressed earlier and more in incompatible than in compatible interactions .
These results suggest that early and high induction of these genes may provide host plants with leading edges to defend themselves .
The localization of two rice PR-10 proteins , PBZ1 and OsPR-10 , was further examined by immunohistochemical analysis .
PBZ1 accumulated highly in mesophyll cells under the attachment site of the appressorium .
In contrast , OsPR-10 expression was mainly localized to vascular it issue . | | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: OsWRKY03 , a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1 .
| | Author: Liu XQ Bai XQ Qian Q Wang XJ Chen MS Chu CC .
| | Journal: Cell Res .
Citation: V : 15 ( 8 ) P : 593-603 Year: 2005 | | Literature: oryza Field: abstract Doc ID: pub16117849 Accession (PMID): 16117849 | | Abstract: WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways .
It has been shown that one Arabidopsis WRKY protein , AtWRKY29/22 , is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens .
However , little is known about the biological roles of WRKY proteins in rice .
In this study , we investigated the expression patterns of rice AtWRKY29/22 homolog , OsWRKY03 , under different conditions , and also its possible role involved in plant defense .
Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments .
Further analysis revealed that the expression of OsWRKY03 was light dependent .
Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay .
Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein .
OsNPR1 as well as several other pathogenesis-related genes , such as OsPR1b , phenylalanine ammonia-lyase ( ZB8 ) and peroxidase ( POX22 . 3 ) , were induced in OsWRKY03-overexpressing transgenic plants .
These results indicated that OsWRKY03 is located upstream of OsNPR1 as a transcriptional activator in salicylic acid ( SA ) -dependent or jasmonic acid ( JA ) -dependent defense signaling cascades . | | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: Rice lesion mimic mutants with enhanced resistance to diseases .
| | Author: Wu C Bordeos A Madamba MR Baraoidan M Ramos M Wang GL Leach JE Leung H | | Journal: Mol Genet Genomics Citation: V : 279 P : 605-19 Year: 2008 | | Literature: oryza Field: abstract Doc ID: pub18357468 Accession (PMID): 18357468 | | Abstract: Lesion mimic mutants are characterized by the formation of necrotic lesions in the absence of pathogens .
Such genetic defects often result in enhanced resistance to pathogen infection and constitutive expression of defense response genes .
To understand the genetic mechanisms leading to these mutations , we characterized 21 lesion mimic mutants isolated from IR64 rice mutant populations produced by mutagenesis with diepoxybutane ( D ) , gamma rays ( G ) , and fast neutrons ( F ) .
Four mutations are controlled by single dominant genes , one of which is inherited maternally .
Five lesion mimics are allelic to known spotted leaf ( spl ) mutants spl1 , spl2 , spl3 , or spl6 .
In total , 11 new lesion mimic mutations , named spl16 , spl17 , and spl19 through Spl27 , were established based on allelism tests .
Two lesion mimics , spl17 and Spl26 showed enhanced resistance to multiple strains of Magnaporthe oryzae , the rice blast pathogen , and Xanthomonas oryzae pv . oryzae , the bacterial blight ( BB ) pathogen .
Co-segregation analyses of blast and BB resistance and lesion mimic phenotypes in segregating populations of spl17 and Spl26 indicate that enhanced resistance to the two diseases is conferred by mutations in the lesion mimic genes .
A double mutant produced from two independent lesion mimics showed more severe lesions and higher level of resistance to X o . pv . oryzae than their single mutant parents indicating a synergistic effect of the two mutations .
In mutants that exhibit enhanced disease resistance to both pathogens , increases in expression of defense response genes PR-10a , POX22 . 3 , and PO-C1 were correlated with lesion mimic development and enhancement of resistance .
These lesion mimic mutants may provide essential materials for a comprehensive dissection of the disease resistance pathways in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: A Raf-like MAPKKK gene DSM1 mediates drought resistance through reactive oxygen species scavenging in rice .
| | Author: Ning J Li X Hicks LM Xiong L | | Journal: Plant Physiol Citation: V : 152 P : 876-90 Year: 2010 | | Literature: oryza Field: abstract Doc ID: pub20007444 Accession (PMID): 20007444 | | Abstract: Mitogen-activated protein kinase ( MAPK ) cascades have been identified in various signaling pathways involved in plant development and stress responses .
We identified a drought-hypersensitive mutant ( drought-hypersensitive mutant1 [ dsm1 ] ) of a putative MAPK kinase kinase ( MAPKKK ) gene in rice ( Oryza sativa ) .
Two allelic dsm1 mutants were more sensitive than wild-type plants to drought stress at both seedling and panicle development stages .
The dsm1 mutants lost water more rapidly than wild-type plants under drought stress , which was in agreement with the increased drought-sensitivity phenotype of the mutant plants .
DSM1-RNA interference lines were also hypersensitive to drought stress .
The predicted DSM1 protein belongs to a B3 subgroup of plant Raf-like MAPKKKs and was localized in the nucleus .
By real-time PCR analysis , the DSM1 gene was induced by salt , drought , and abscisic acid , but not by cold .
Microarray analysis revealed that two peroxidase ( POX ) genes , POX22 . 3 and POX8 . 1 , were sharply down-regulated compared to wild type , suggesting that DSM1 may be involved in reactive oxygen species ( ROS ) signaling .
Peroxidase activity , electrolyte leakage , chlorophyll content , and 3 , 3-diaminobenzidine staining revealed that the dsm1 mutant was more sensitive to oxidative stress due to an increase in ROS damage caused by the reduced POX activity .
Overexpression of DSM1 in rice increased the tolerance to dehydration stress at the seedling stage .
Together , these results suggest that DSM1 might be a novel MAPKKK functioning as an early signaling component in regulating responses to drought stress by regulating scavenging of ROS in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: Rice 14-3-3 protein ( GF14e ) negatively affects cell death and disease resistance .
| | Author: Manosalva PM Bruce M Leach JE | | Journal: Plant J Citation: V : 68 P : 777-87 Year: 2011 | | Literature: oryza Field: abstract Doc ID: pub21793954 Accession (PMID): 21793954 | | Abstract: Plant 14-3-3 proteins regulate important cellular processes , including plant immune responses , through protein-protein interactions with a wide range of target proteins .
In rice ( Oryza sativa ) , the GF14e gene , which encodes a 14-3-3 protein , is induced during effector-triggered immunity ( ETI ) associated with pathogens such as Xanthomonas oryzae pv . oryzae ( Xoo ) .
To determine whether the GF14e gene plays a direct role in resistance to disease in rice , we suppressed its expression by RNAi silencing .
GF14e suppression was correlated with the appearance of a lesion-mimic ( LM ) phenotype in the transgenic plants at 3 weeks after sowing .
This indicates inappropriate regulation of cell death , a phenotype that is frequently associated with enhanced resistance to pathogens .
GF14e-silenced rice plants showed high levels of resistance to a virulent strain of Xoo compared with plants that were not silenced .
Enhanced resistance was correlated with GF14e silencing prior to and after development of the LM phenotype , higher basal expression of a defense response peroxidase gene ( POX22 . 3 ) , and accumulation of reactive oxygen species ( ROS ) .
In addition , GF14e-silenced plants also exhibit enhanced resistance to the necrotrophic fungal pathogen Rhizoctonia solani .
Together , our findings suggest that GF14e negatively affects the induction of plant defense response genes , cell death and broad-spectrum resistance in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
