2411 matches found in 1927 documents. Search time: 0.071 seconds. |
|
Score: 1.00 | Title: A rice homolog of Cdk7/MO15 phosphorylates both cyclin-dependent protein kinases and the carboxy-terminal domain of RNA polymerase II .
| Author: Yamaguchi M Umeda M Uchimiya H | Journal: Plant J Citation: V : 16 ( 5 ) P : 613-9 Year: 1998 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10036778 Accession (PMID): 10036778 | Abstract: The activation of cyclin-dependent protein kinases ( CDKs ) requires phosphorylation of a threonine residue within the T-loop by a CDK-activating kinase ( CAK ) .
The R2 protein of rice is very similar to CAKs of animals and fission yeast at the amino acid level but phosphorylation by R2 has not yet been demonstrated .
When R2 was overexpressed in a CAK-deficient mutant of budding yeast , it suppressed the temperature sensitivity of the mutation .
Immunoprecipitates of rice proteins with the anti-R2 antibody phosphorylated human CDK2 , one of the rice CDKs ( Cdc2Os1 ) , and the carboxy-terminal domain ( CTD ) of the largest subunit of RNA polymerase II of Arabidopsis .
Mutational analysis indicated that R2 phosphorylated the threonine residue within the T-loop of CDK2 and Cdc2Os1 .
R2 was found mainly in two protein complexes which had molecular masses of 190 kDa and 70 kDa , respectively , whilst the CDK and CTD-kinase activities associated with R2 were identified in a complex of 105 kDa .
These results indicate that R2 is closely related to CAKs of animals and fission yeast in terms of its phosphorylation activity and , moreover , that this CAK of rice is distinct from a CAK of the dicotyledonous plant Arabidopsis . | Matching Sentences: [ Sen. 6, subscore: 1.00 ]: The R2 protein of rice is very similar to CAKs of animals and fission yeast at the amino acid level but phosphorylation by R2 has not yet been demonstrated . When R2 was overexpressed in a CAK-deficient mutant of budding yeast , it suppressed the temperature sensitivity of the mutation . Immunoprecipitates of rice proteins with the anti-R2 antibody phosphorylated human CDK2 , one of the rice CDKs ( Cdc2Os1 ) , and the carboxy-terminal domain ( CTD ) of the largest subunit of RNA polymerase II of Arabidopsis . Mutational analysis indicated that R2 phosphorylated the threonine residue within the T-loop of CDK2 and Cdc2Os1 . R2 was found mainly in two protein complexes which had molecular masses of 190 kDa and 70 kDa , respectively , whilst the CDK and CTD-kinase activities associated with R2 were identified in a complex of 105 kDa . These results indicate that R2 is closely related to CAKs of animals and fission yeast in terms of its phosphorylation activity and , moreover , that this CAK of rice is distinct from a CAK of the dicotyledonous plant Arabidopsis .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 | Title: The status of ORT ( oral rehydration therapy ) in Bangladesh : how widely is it used?
| Author: Chowdhury AM Karim F Sarkar SK Cash RA Bhuiya A | Journal: Citation: V : 12 ( 1 ) P : 58-66 Year: 1997 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10166103 Accession (PMID): 10166103 | Abstract: During 1980-1990 BRAC , a Bangladeshi non-governmental organization , taught over 12 million mothers how to prepare oral rehydration therapy ( ORT ) at home with lobon ( common salt ) and gur ( unrefined brown sugar ) .
This was followed by a strong promotion and distribution of prepackaged ORS by various agencies including the government .
In 1993 we assessed knowledge of ORT preparation , its local availability and its use for the management of diarrhoea .
Over 9000 households in 90 villages were revisited ; 306 government outreach health workers , 296 drug sellers , and 237 village doctors were interviewed ; 152 government facilities and 495 pharmacies/shops were visited .
ORT prepared by mothers in a sub-sample of the households was analyzed for chloride content and interviewers collected information on use of ORT for diarrhoeal episodes occurring in the preceding two weeks .
The data quality was assessed through a resurvey of sample respondents within two weeks of the first interview .
Over 70% of the mothers could prepare a chemically safe and effective ORS .
A significant proportion of these mothers were very young at the time of the mass campaigns using house to house teaching , implying an intergenerational transfer of the knowledge on ORT .
ORT was found to be used in 60% of all diarrhoeal episodes , but the rate varied with the type of diarrhoea , being highest for daeria ( severe watery diarrhoea ) and lowest for amasha ( dysentery ) .
Drug sellers and village doctors now recommend ORT much more frequently than before .
Members of the medical profession ( qualified and unqualified ) still lag behind in prescribing the use of ORT .
The availability of pre-packaged ORS in rural pharmacies has improved enormously .
There is convincing evidence that the widescale promotion in the past of ORS for dehydration in diarrhoea has led to this marked improvement today .
Nevertheless the use of rice-based ORS , culturally appropriate messages and the promotion of ORS with food offer opportunities to further improve the utilization of ORT .
| Matching Sentences: [ Sen. 8, subscore: 1.00 ]: Over 9000 households in 90 villages were revisited ; 306 government outreach health workers , 296 drug sellers , and 237 village doctors were interviewed ; 152 government facilities and 495 pharmacies/shops were visited . ORT prepared by mothers in a sub-sample of the households was analyzed for chloride content and interviewers collected information on use of ORT for diarrhoeal episodes occurring in the preceding two weeks . The data quality was assessed through a resurvey of sample respondents within two weeks of the first interview . Over 70% of the mothers could prepare a chemically safe and effective ORS . A significant proportion of these mothers were very young at the time of the mass campaigns using house to house teaching , implying an intergenerational transfer of the knowledge on ORT . ORT was found to be used in 60% of all diarrhoeal episodes , but the rate varied with the type of diarrhoea , being highest for daeria ( severe watery diarrhoea ) and lowest for amasha ( dysentery ) . Drug sellers and village doctors now recommend ORT much more frequently than before . Members of the medical profession ( qualified and unqualified ) still lag behind in prescribing the use of ORT . The availability of pre-packaged ORS in rural pharmacies has improved enormously .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 | Title: Genetic effects on weight change and food intake in Swedish adult twins .
| Author: Heitmann BL Harris JR Lissner L Pedersen NL .
| Journal: Am .
J Clin . Nutr . Citation: V : 69 ( 4 ) P : 597-602 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10197559 Accession (PMID): 10197559 | Abstract: BACKGROUND : Obesity is influenced by genetic and environmental factors .
Additionally , synergistic effects of genes and environments may be important in the development of obesity .
OBJECTIVE : The aim of this study was to test for genetic effects on food consumption frequency , food preferences , and their interaction with subsequent weight gain .
DESIGN : Complete data on the frequencies of consumption of 11 foods typical of the Swedish diet were available for 98 monozygotic and 176 dizygotic twin pairs aged 25-59 y who are part of the Swedish Twin Registry .
The data were collected in 1973 as part of a questionnaire study .
Body mass index was measured in 1973 and again in 1984 .
RESULTS : There was some evidence that genetic effects influenced the frequency of intake of some foods .
Similarity among monozygotic twins exceeded that among dizygotic twins for intake of flour and grain products and fruit in men and women , intake of milk in men , and intake of vegetables and rice in women , suggesting that genes influence preferences for these foods .
Analyses conducted for twins reared together and apart also suggested greater monozygotic than dizygotic correlations , but cross-twin , cross-trait correlations were all insignificant , suggesting that the genes that affect consumption frequencies are not responsible for mediating the relation between the frequency of intake and weight change .
CONCLUSIONS : Genetic effects and the frequency of intake are independently related to change in body mass index However , there was no suggestion of differential genetic effects on weight gain that were dependent on the consumption frequency of the foods studied .
| Matching Sentences: [ Sen. 6, subscore: 1.00 ]: Additionally , synergistic effects of genes and environments may be important in the development of obesity . OBJECTIVE : The aim of this study was to test for genetic effects on food consumption frequency , food preferences , and their interaction with subsequent weight gain . DESIGN : Complete data on the frequencies of consumption of 11 foods typical of the Swedish diet were available for 98 monozygotic and 176 dizygotic twin pairs aged 25-59 y who are part of the Swedish Twin Registry . The data were collected in 1973 as part of a questionnaire study . Body mass index was measured in 1973 and again in 1984 . RESULTS : There was some evidence that genetic effects influenced the frequency of intake of some foods . Similarity among monozygotic twins exceeded that among dizygotic twins for intake of flour and grain products and fruit in men and women , intake of milk in men , and intake of vegetables and rice in women , suggesting that genes influence preferences for these foods . Analyses conducted for twins reared together and apart also suggested greater monozygotic than dizygotic correlations , but cross-twin , cross-trait correlations were all insignificant , suggesting that the genes that affect consumption frequencies are not responsible for mediating the relation between the frequency of intake and weight change . CONCLUSIONS : Genetic effects and the frequency of intake are independently related to change in body mass index However , there was no suggestion of differential genetic effects on weight gain that were dependent on the consumption frequency of the foods studied . [ Sen. 10, subscore: 1.00 ]: Body mass index was measured in 1973 and again in 1984 . RESULTS : There was some evidence that genetic effects influenced the frequency of intake of some foods . Similarity among monozygotic twins exceeded that among dizygotic twins for intake of flour and grain products and fruit in men and women , intake of milk in men , and intake of vegetables and rice in women , suggesting that genes influence preferences for these foods . Analyses conducted for twins reared together and apart also suggested greater monozygotic than dizygotic correlations , but cross-twin , cross-trait correlations were all insignificant , suggesting that the genes that affect consumption frequencies are not responsible for mediating the relation between the frequency of intake and weight change . CONCLUSIONS : Genetic effects and the frequency of intake are independently related to change in body mass index However , there was no suggestion of differential genetic effects on weight gain that were dependent on the consumption frequency of the foods studied .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 | Title: Isolation and characterization of the gene encoding the starch debranching enzyme limit dextrinase from germinating barley .
| Author: Kristensen M Lok F Planchot V Svendsen I Leah R Svensson B | Journal: Biochim . Biophys . Acta Citation: V : 1431 ( 2 ) P : 538-46 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10350630 Accession (PMID): 10350630 | Abstract: The gene encoding the starch debranching enzyme limit dextrinase , LD , from barley ( Hordeum vulgare ) , was isolated from a genomic phage library using a barley cDNA clone as probe .
The gene encodes a protein of 904 amino acid residues with a calculated molecular mass of 98 . 6 kDa .
This is in agreement with a value of 105 kDa estimated by SDS-PAGE .
The coding sequence is interrupted by 26 introns varying in length from 93 bp to 825 bp .
The 27 exons vary in length from 53 bp to 197 bp .
Southern blot analysis shows that the limit dextrinase gene is present as a single copy in the barley genome .
Gene expression is high during germination and the steady state transcription level reaches a maximum at day 5 of germination .
The deduced amino acid sequence corresponds to the protein sequence of limit dextrinase purified from germinating malt , as determined by automated N-terminal sequencing of tryptic fragments coupled with matrix assisted laser desorption mass spectrometry .
The sequenced peptide fragments cover 70% of the entire protein sequence , which shows 62% and 77% identity to that of starch debranching enzymes from spinach and rice and 37% identity to Klebsiella pullulanase .
Sequence alignment supports the multidomain architecture and identifies both secondary structure elements of the catalytic ( beta/alpha ) 8-barrel substrate , catalytic residues , and specificity associated motifs characteristic of members of the glycoside hydrolase family 13 which cleave alpha-1 , 6-glucosidic bonds .
A remarkable distribution of the secondary structure elements to individual exons is observed .
| Matching Sentences: [ Sen. 2, subscore: 1.00 ]: The gene encoding the starch debranching enzyme limit dextrinase , LD , from barley ( Hordeum vulgare ) , was isolated from a genomic phage library using a barley cDNA clone as probe . The gene encodes a protein of 904 amino acid residues with a calculated molecular mass of 98 . 6 kDa . This is in agreement with a value of 105 kDa estimated by SDS-PAGE . The coding sequence is interrupted by 26 introns varying in length from 93 bp to 825 bp . The 27 exons vary in length from 53 bp to 197 bp . Southern blot analysis shows that the limit dextrinase gene is present as a single copy in the barley genome . [ Sen. 8, subscore: 1.00 ]: The coding sequence is interrupted by 26 introns varying in length from 93 bp to 825 bp . The 27 exons vary in length from 53 bp to 197 bp . Southern blot analysis shows that the limit dextrinase gene is present as a single copy in the barley genome . Gene expression is high during germination and the steady state transcription level reaches a maximum at day 5 of germination . The deduced amino acid sequence corresponds to the protein sequence of limit dextrinase purified from germinating malt , as determined by automated N-terminal sequencing of tryptic fragments coupled with matrix assisted laser desorption mass spectrometry . The sequenced peptide fragments cover 70% of the entire protein sequence , which shows 62% and 77% identity to that of starch debranching enzymes from spinach and rice and 37% identity to Klebsiella pullulanase . Sequence alignment supports the multidomain architecture and identifies both secondary structure elements of the catalytic ( beta/alpha ) 8-barrel substrate , catalytic residues , and specificity associated motifs characteristic of members of the glycoside hydrolase family 13 which cleave alpha-1 , 6-glucosidic bonds . A remarkable distribution of the secondary structure elements to individual exons is observed .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 | Title: The evaluation of antianaphylactic effect of Oryza sativa L in rats .
| Author: Kim HM Yi DK Shin HY .
| Journal: Am .
J Chin . Med . Citation: V : 27 ( 1 ) P : 63-71 Year: Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10354818 Accession (PMID): 10354818 | Abstract: This study was carried out to examine the effect of methanol extract of Oryza sativa L ( Dong-Jin in Korean , abbreviate as Os-DJ hereafter ) on anaphylaxis .
Os-DJ ( 10 ( -5 ) to 1 g/kg ) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats .
When Os-DJ was pretreated at concentration ranging from 10 ( -5 ) to 1 g/kg , the serum histamine levels were reduced in a dose-dependent manner .
Os-DJ ( 1 g/kg ) also significantly inhibited local anaphylaxis activated by anti-dinitrophenyl ( DNP ) IgE .
Moreover , Os-DJ dose-dependently inhibited the histamine release from rat peritoneal mast cells ( RPMC ) activated by compound 48/80 or anti-DNP IgE .
These results indicate that Os-DJ possess antianaphylactic activity by inhibition of histamine release from mast cells in vivo and in vitro . | Matching Sentences: [ Sen. 5, subscore: 1.00 ]: This study was carried out to examine the effect of methanol extract of Oryza sativa L ( Dong-Jin in Korean , abbreviate as Os-DJ hereafter ) on anaphylaxis . Os-DJ ( 10 ( -5 ) to 1 g/kg ) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats . When Os-DJ was pretreated at concentration ranging from 10 ( -5 ) to 1 g/kg , the serum histamine levels were reduced in a dose-dependent manner . Os-DJ ( 1 g/kg ) also significantly inhibited local anaphylaxis activated by anti-dinitrophenyl ( DNP ) IgE . Moreover , Os-DJ dose-dependently inhibited the histamine release from rat peritoneal mast cells ( RPMC ) activated by compound 48/80 or anti-DNP IgE . These results indicate that Os-DJ possess antianaphylactic activity by inhibition of histamine release from mast cells in vivo and in vitro . [ Sen. 6, subscore: 1.00 ]: Os-DJ ( 10 ( -5 ) to 1 g/kg ) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats . When Os-DJ was pretreated at concentration ranging from 10 ( -5 ) to 1 g/kg , the serum histamine levels were reduced in a dose-dependent manner . Os-DJ ( 1 g/kg ) also significantly inhibited local anaphylaxis activated by anti-dinitrophenyl ( DNP ) IgE . Moreover , Os-DJ dose-dependently inhibited the histamine release from rat peritoneal mast cells ( RPMC ) activated by compound 48/80 or anti-DNP IgE . These results indicate that Os-DJ possess antianaphylactic activity by inhibition of histamine release from mast cells in vivo and in vitro .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |