Score: 2.00 | Title: Characterization of the rice pathogen-related protein Rir1a and regulation of the corresponding gene .
| Author: Mauch F Reimmann C Freydl E Schaffrath U Dudler R | Journal: Plant Mol . Biol . Citation: V : 38 ( 4 ) P : 577-86 Year: 1998 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub9747803 Accession (PMID): 9747803 | Abstract: In rice ( Oryza sativa L ) , local acquired resistance against Pyricularia oryzae ( Cav . ) , the causal agent of rice blast , can be induced by a preinoculation with the non-host pathogen Pseudomonas syringae pv . syringae .
We have cloned a cDNA ( Rir1a ) and a closely related gene ( Rir1b ) corresponding to transcripts that accumulate in leaf it issue upon inoculation with P syringae pv . syringae .
The cDNA encodes a putative 107 amino acid protein , Rir1a , that exhibits a putative signal peptide cleavage site in its hydrophobic N-terminal part and a C-terminal part that is relatively rich in glycine and proline .
The Rir1b gene contains a Tourist and a Wanderer miniature transposable element in its single intron and encodes a nearly identical protein .
Rir1a is similar in sequence ( ca .
35% identical and ca 60% conservatively changed amino acids ) to the putative Wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat .
Using antibodies raised against a Rir1a-fusion protein we show that Rir1a is secreted from rice protoplasts transiently expressing a 35S : : Rir1a construct and that the protein accumulates in the cell wall compartment of rice leaves upon inoculation with P syringae pv . syringae .
Possible roles of Rir1a in pathogen defense are discussed . | Matching Sentences: [ Sen. 2, subscore: 1.00 ]: In rice ( Oryza sativa L ) , local acquired resistance against Pyricularia oryzae ( Cav . ) , the causal agent of rice blast , can be induced by a preinoculation with the non-host pathogen Pseudomonas syringae pv . syringae . We have cloned a cDNA ( Rir1a ) and a closely related gene ( Rir1b ) corresponding to transcripts that accumulate in leaf it issue upon inoculation with P syringae pv . syringae . The cDNA encodes a putative 107 amino acid protein , Rir1a , that exhibits a putative signal peptide cleavage site in its hydrophobic N-terminal part and a C-terminal part that is relatively rich in glycine and proline . The Rir1b gene contains a Tourist and a Wanderer miniature transposable element in its single intron and encodes a nearly identical protein . Rir1a is similar in sequence ( ca . 35% identical and ca 60% conservatively changed amino acids ) to the putative Wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat . Using antibodies raised against a Rir1a-fusion protein we show that Rir1a is secreted from rice protoplasts transiently expressing a 35S : : Rir1a construct and that the protein accumulates in the cell wall compartment of rice leaves upon inoculation with P syringae pv . syringae . Possible roles of Rir1a in pathogen defense are discussed . [ Sen. 4, subscore: 1.00 ]: In rice ( Oryza sativa L ) , local acquired resistance against Pyricularia oryzae ( Cav . ) , the causal agent of rice blast , can be induced by a preinoculation with the non-host pathogen Pseudomonas syringae pv . syringae . We have cloned a cDNA ( Rir1a ) and a closely related gene ( Rir1b ) corresponding to transcripts that accumulate in leaf it issue upon inoculation with P syringae pv . syringae . The cDNA encodes a putative 107 amino acid protein , Rir1a , that exhibits a putative signal peptide cleavage site in its hydrophobic N-terminal part and a C-terminal part that is relatively rich in glycine and proline . The Rir1b gene contains a Tourist and a Wanderer miniature transposable element in its single intron and encodes a nearly identical protein . Rir1a is similar in sequence ( ca . 35% identical and ca 60% conservatively changed amino acids ) to the putative Wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat . Using antibodies raised against a Rir1a-fusion protein we show that Rir1a is secreted from rice protoplasts transiently expressing a 35S : : Rir1a construct and that the protein accumulates in the cell wall compartment of rice leaves upon inoculation with P syringae pv . syringae . Possible roles of Rir1a in pathogen defense are discussed .
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Score: 1.00 | Title: Constitutive expression of the defense-related Rir1b gene in transgenic rice plants confers enhanced resistance to the rice blast fungus Magnaporthe grisea .
| Author: Schaffrath U Mauch F Freydl E Schweizer P Dudler R | Journal: Plant Mol . Biol . Citation: V : 43 ( 1 ) P : 59-66 Year: 2000 Type: ARTICLE | Literature: oryza Field: title Doc ID: pub10949374 Accession (PMID): 10949374 | Abstract: The Rirlb gene of rice ( Oryza sativa ) is one of a set of putative defense genes whose transcripts accumulate upon inoculation of rice with the non-host pathogen Pseudomonas syringae pv . syringae .
It belongs to a family of genes encoding small extracellular proteins so far only identified in cereals .
To assess the function of the Rirlb gene in rice blast resistance , it was placed under the control of the CaMV 35S promoter and transferred into rice plants of the japonica variety Taipei 309 by biolistic transformation of immature embryos .
Two out of 12 hygromycin-resistant regenerated plants ( OE1 and OE3 ) were fertile .
DNA gel blot analysis suggested that these two T0 plants were independent transformants , each of which had stably incorporated one complete copy of the transgene into the genome .
In addition , the OE1 plant appeared also a contain a rearranged copy or incomplete copy .
T1 plants homozygous for the transgene were identified by DNA gel blot analysis of individual T2 progeny and further propagated .
Expression analysis of the transgene showed that the transgene was active both in T1 plants and homozygous decendants .
Challenge inoculation of homozygous transgenic plants with Magnaporthe grisea , the causal agent of rice blast disease , revealed that both independent transgenic lines were more resistant than the untransformed wild type , suggesting that over-expression of the Rirlb gene confers partial resistance against this important pathogen . | Matching Sentences: [ Sen. 1, subscore: 1.00 ]: Constitutive expression of the defense-related Rir1b gene in transgenic rice plants confers enhanced resistance to the rice blast fungus Magnaporthe grisea .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |