| 29 matches found in 16 documents. Search time: 0.372 seconds. |
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| Title: Glucose modulates the abscisic acid-inducible Rab16A gene in cereal embryos .
| | Author: Toyofuku K Loreti E Vernieri P Alpi A Perata P Yamaguchi J | | Journal: Plant Mol . Biol . Citation: V : 42 ( 3 ) P : 451-60 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10798615 Accession (PMID): 10798615 | | Abstract: Glucose effects on the expression of the abscisic acid-inducible Rab16A gene were examined in rice and barley embryos .
Glucose feeding to rice embryos negatively affects the endogenous abscisic acid content and represses the promoter activity of the Rab16A gene .
Glucose repression of the Rab16A gene takes place both at a transcriptional and a post-transcriptional level .
Modulation of the abscisic acid content in rice embryos triggered by glucose did not directly influence the expression of the rice alpha-amylase gene RAmy3D , which is known to be under glucose control .
The possible interaction between the glucose and abscisic acid signaling pathway is discussed . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Glucose effects on the expression of the abscisic acid-inducible Rab16A gene were examined in rice and barley embryos . Glucose feeding to rice embryos negatively affects the endogenous abscisic acid content and represses the promoter activity of the Rab16A gene . Glucose repression of the Rab16A gene takes place both at a transcriptional and a post-transcriptional level . Modulation of the abscisic acid content in rice embryos triggered by glucose did not directly influence the expression of the rice alpha-amylase gene RAmy3D , which is known to be under glucose control . The possible interaction between the glucose and abscisic acid signaling pathway is discussed .
[ Sen. 2, subscore: 1.00 ]: Glucose effects on the expression of the abscisic acid-inducible Rab16A gene were examined in rice and barley embryos . Glucose feeding to rice embryos negatively affects the endogenous abscisic acid content and represses the promoter activity of the Rab16A gene . Glucose repression of the Rab16A gene takes place both at a transcriptional and a post-transcriptional level . Modulation of the abscisic acid content in rice embryos triggered by glucose did not directly influence the expression of the rice alpha-amylase gene RAmy3D , which is known to be under glucose control . The possible interaction between the glucose and abscisic acid signaling pathway is discussed .
[ Sen. 3, subscore: 1.00 ]: Glucose effects on the expression of the abscisic acid-inducible Rab16A gene were examined in rice and barley embryos . Glucose feeding to rice embryos negatively affects the endogenous abscisic acid content and represses the promoter activity of the Rab16A gene . Glucose repression of the Rab16A gene takes place both at a transcriptional and a post-transcriptional level . Modulation of the abscisic acid content in rice embryos triggered by glucose did not directly influence the expression of the rice alpha-amylase gene RAmy3D , which is known to be under glucose control . The possible interaction between the glucose and abscisic acid signaling pathway is discussed .
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Score: 1.00 |
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| Title: A Ca ( 2+ ) -dependent protein kinase that endows rice plants with cold and salt-stress tolerance functions in vascular bundles .
| | Author: Saijo Y Kinoshita N Ishiyama K Hata S Kyozuka J Hayakawa T Nakamura T Shimamoto K Yamaya T Izui K | | Journal: Plant Cell Physiol .
Citation: V : 42 ( 11 ) P : 1228-33 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11726707 Accession (PMID): 11726707 | | Abstract: A rice Ca ( 2+ ) -dependent protein kinase , OsCDPK7 , is a positive regulator commonly involved in the tolerance to cold and salt/drought .
We carried out in situ detection of the transcript and immunolocalization of the protein .
In the wild-type rice plants under both stress conditions , OsCDPK7 was expressed predominantly in vascular it issues of crowns and roots , vascular bundles and central cylinder , respectively , where water stress occurs most severely .
This enzyme was also expressed in the peripheral cylinder of crown vascular bundles and root sclerenchyma .
Similar localization patterns with stronger signals were observed in stress-tolerant OsCDPK7 over-expressing transformants with the cauliflower mosaic virus 35S promoter .
The transcript of a putative target gene of the OsCDPK7 signaling pathway , rab16A , was also detected essentially in the same it issues upon salt stress , suggesting that the OsCDPK7 pathway operates predominantly in these regions .
We propose that the use of the 35S promoter fortuitously strengthened the localized expression of OsCDPK7 , resulting in enhancement of the stress signaling in the inherently operating regions leading to improved stress tolerance . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: A rice Ca ( 2+ ) -dependent protein kinase , OsCDPK7 , is a positive regulator commonly involved in the tolerance to cold and salt/drought . We carried out in situ detection of the transcript and immunolocalization of the protein . In the wild-type rice plants under both stress conditions , OsCDPK7 was expressed predominantly in vascular it issues of crowns and roots , vascular bundles and central cylinder , respectively , where water stress occurs most severely . This enzyme was also expressed in the peripheral cylinder of crown vascular bundles and root sclerenchyma . Similar localization patterns with stronger signals were observed in stress-tolerant OsCDPK7 over-expressing transformants with the cauliflower mosaic virus 35S promoter . The transcript of a putative target gene of the OsCDPK7 signaling pathway , rab16A , was also detected essentially in the same it issues upon salt stress , suggesting that the OsCDPK7 pathway operates predominantly in these regions . We propose that the use of the 35S promoter fortuitously strengthened the localized expression of OsCDPK7 , resulting in enhancement of the stress signaling in the inherently operating regions leading to improved stress tolerance .
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Score: 1.00 |
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| Title: The slender rice mutant , with constitutively activated gibberellin signal transduction , has enhanced capacity for abscisic acid level .
| | Author: Ikeda A Sonoda Y Vernieri P Perata P Hirochika H Yamaguchi J | | Journal: Plant Cell Physiol .
Citation: V : 43 ( 9 ) P : 974-9 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12354914 Accession (PMID): 12354914 | | Abstract: The slender rice ( slr1-1 ) mutant , carrying a lethal and recessive single mutation , has a constitutive gibberellin ( GA ) -response phenotype and behaves as if it were saturated with GAs [ Ikeda et al ( 2001 ) Plant Cell 13 , 999 ] .
The SLR1 gene , with sequence homology to members of the plant-specific GRAS gene family , is a mediator of the GA signal transduction process .
In the slender rice , GA-inducible alpha-amylase was produced from the aleurone layer without applying GA .
GA-independent alpha-amylase production in the mutant was inhibited by applying abscisic acid ( ABA ) .
Shoot elongation in the mutant was also suppressed by ABA , indicating that the slender rice responds normally to ABA .
Interestingly , shoot ABA content was 10-fold higher in the mutant than in the wild type , while there was no difference in root ABA content .
Expression of the Rab16A gene , which is known to be ABA inducible , was about 10-fold higher in shoots of the mutant than in those of the wild type .
These results indicate that constitutive activation of the GA signal transduction pathway by the slr1-1 mutation promotes the endogenous ABA level . | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The slender rice ( slr1-1 ) mutant , carrying a lethal and recessive single mutation , has a constitutive gibberellin ( GA ) -response phenotype and behaves as if it were saturated with GAs [ Ikeda et al ( 2001 ) Plant Cell 13 , 999 ] . The SLR1 gene , with sequence homology to members of the plant-specific GRAS gene family , is a mediator of the GA signal transduction process . In the slender rice , GA-inducible alpha-amylase was produced from the aleurone layer without applying GA . GA-independent alpha-amylase production in the mutant was inhibited by applying abscisic acid ( ABA ) . Shoot elongation in the mutant was also suppressed by ABA , indicating that the slender rice responds normally to ABA . Interestingly , shoot ABA content was 10-fold higher in the mutant than in the wild type , while there was no difference in root ABA content . Expression of the Rab16A gene , which is known to be ABA inducible , was about 10-fold higher in shoots of the mutant than in those of the wild type . These results indicate that constitutive activation of the GA signal transduction pathway by the slr1-1 mutation promotes the endogenous ABA level .
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Score: 1.00 |
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| Title: Expression of a calcineurin gene improves salt stress tolerance in transgenic rice .
| | Author: Ma X Qian Q Zhu D | | Journal: Plant Mol . Biol . Citation: V : 58 ( 4 ) P : 483-95 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16021334 Accession (PMID): 16021334 | | Abstract: Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase and has multiple functions in animal cells including regulating ionic homeostasis .
We generated transgenic rice plants that not only expressed a truncated form of the catalytic subunit of mouse calcineurin , but also were able to grow and fertilize normally in the field .
Notably , the expression of the mouse calcineurin gene in rice resulted in its higher salt stress tolerance than the non-transgenic rice .
Physiological studies have indicated that the root growth of transgenic plants was less inhibited than the shoot growth , and that less Na+ was accumulated in the roots of transgenic plants after a prolonged period of salt stress .
These findings imply that the heterologous calcineurin plays a significant role in maintaining ionic homeostasis and the integrity of plant roots when exposed to salt .
In addition , the calcineurin gene expression in the stems of transgenic plants correlated with the increased expression of the Rab16A gene that encodes a group 2-type late-embryogenesis-abundant ( LEA ) protein .
Altogether our findings provide the first genetic and physiological evidence that expression of the mouse calcineurin protein functionally improves the salt stress tolerance of rice partly by limiting Na+ accumulation in the roots . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase and has multiple functions in animal cells including regulating ionic homeostasis . We generated transgenic rice plants that not only expressed a truncated form of the catalytic subunit of mouse calcineurin , but also were able to grow and fertilize normally in the field . Notably , the expression of the mouse calcineurin gene in rice resulted in its higher salt stress tolerance than the non-transgenic rice . Physiological studies have indicated that the root growth of transgenic plants was less inhibited than the shoot growth , and that less Na+ was accumulated in the roots of transgenic plants after a prolonged period of salt stress . These findings imply that the heterologous calcineurin plays a significant role in maintaining ionic homeostasis and the integrity of plant roots when exposed to salt . In addition , the calcineurin gene expression in the stems of transgenic plants correlated with the increased expression of the Rab16A gene that encodes a group 2-type late-embryogenesis-abundant ( LEA ) protein . Altogether our findings provide the first genetic and physiological evidence that expression of the mouse calcineurin protein functionally improves the salt stress tolerance of rice partly by limiting Na+ accumulation in the roots .
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Score: 1.00 |
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| Title: An ABRE-binding factor , OSBZ8 , is highly expressed in salt tolerant cultivars than in salt sensitive cultivars of indica rice .
| | Author: Mukherjee K Choudhury AR Gupta B Gupta S Sengupta DN .
| | Journal: BMC Plant Biol .
Citation: V : 6 ( ) P : 18 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16939657 Accession (PMID): 16939657 | | Abstract: BACKGROUND : The bZIP class Abscisic acid Responsive Element ( ABRE ) -binding factor , OSBZ8 ( 38 . 5 kD ) has been considered to regulate ABA-mediated transcription in the suspension cultured cells of japonica rice .
Still , nothing is known about the expression of OSBZ8 at protein level in vegetative it issue of salt sensitive and salt tolerant rice plants .
In our previous study , Electrophoretic Mobility Shift Assay ( EMSA ) of [ 32P ] ABRE-DNA and nuclear extracts prepared from the lamina of Pokkali rice plants has detected the presence of an ABRE-binding factor .
Northern analysis has also detected salinity stress induced accumulation of transcripts for bZIP class of factor .
Therefore , OSBZ8 was considered to play an important role in the regulation of transcription in the vegetative it issue of rice .
The aim of this study is to find out whether OSBZ8 has any role in regulating the NaCl-stress induced gene expression in vegetative it issue and whether the expression of OSBZ8 factor directly correlates with the stress tolerance of different varieties of indica type rice .
RESULTS : Northern analysis of total RNA from roots and lamina of salt-sensitive M-I-48 and salt-tolerant Nonabokra , when probed with the N-terminal unique region of OSBZ8 ( OSBZ8p , without the highly conserved basic region ) , a transcript of 1 . 3 kb hybridized and its level was much higher in tolerant cultivar .
EMSA with Em1a , the strongest ABA Responsive Element till reported from the upstream of EmBP1 , and the nuclear extracts from laminar it issue of untreated and salt-treated seedlings of three salt sensitive , one moderately sensitive and two salt tolerant indica rice cultivars showed specific binding of nuclear factor to ABRE element .
Intensity of binding was low and inducible in salt sensitive rice cultivars while high and constitutive in salt tolerant cultivars .
EMSA with 300 bp 5upstream region of Rab16A gene , a well known salt stress and ABA-inducible gene of rice , showed formation of two complexes , again very weak in salt sensitive and strong in salt tolerant rice cultivar .
CONCLUSION : The bZIP factor OSBZ8 was found to be present in the ABRE-DNA : protein complex as shown by the supershift of the complex by the purified antiserum raised against OSBZ8p .
Treatment of the seedlings with NaCl was found to enhance the complex formation , suggesting the regulation of OSBZ8 gene at both transcriptional and post-translational steps .
Comparative EMSA with different varieties of rice suggests a positive correlation with the expression pattern of OSBZ8 and salt tolerance in rice cultivars . | Matching Sentences:
[ Sen. 10, subscore: 1.00 ]: BACKGROUND : The bZIP class Abscisic acid Responsive Element ( ABRE ) -binding factor , OSBZ8 ( 38 . 5 kD ) has been considered to regulate ABA-mediated transcription in the suspension cultured cells of japonica rice . Still , nothing is known about the expression of OSBZ8 at protein level in vegetative it issue of salt sensitive and salt tolerant rice plants . In our previous study , Electrophoretic Mobility Shift Assay ( EMSA ) of [ 32P ] ABRE-DNA and nuclear extracts prepared from the lamina of Pokkali rice plants has detected the presence of an ABRE-binding factor . Northern analysis has also detected salinity stress induced accumulation of transcripts for bZIP class of factor . Therefore , OSBZ8 was considered to play an important role in the regulation of transcription in the vegetative it issue of rice . The aim of this study is to find out whether OSBZ8 has any role in regulating the NaCl-stress induced gene expression in vegetative it issue and whether the expression of OSBZ8 factor directly correlates with the stress tolerance of different varieties of indica type rice . RESULTS : Northern analysis of total RNA from roots and lamina of salt-sensitive M-I-48 and salt-tolerant Nonabokra , when probed with the N-terminal unique region of OSBZ8 ( OSBZ8p , without the highly conserved basic region ) , a transcript of 1 . 3 kb hybridized and its level was much higher in tolerant cultivar . EMSA with Em1a , the strongest ABA Responsive Element till reported from the upstream of EmBP1 , and the nuclear extracts from laminar it issue of untreated and salt-treated seedlings of three salt sensitive , one moderately sensitive and two salt tolerant indica rice cultivars showed specific binding of nuclear factor to ABRE element . Intensity of binding was low and inducible in salt sensitive rice cultivars while high and constitutive in salt tolerant cultivars . EMSA with 300 bp 5upstream region of Rab16A gene , a well known salt stress and ABA-inducible gene of rice , showed formation of two complexes , again very weak in salt sensitive and strong in salt tolerant rice cultivar . CONCLUSION : The bZIP factor OSBZ8 was found to be present in the ABRE-DNA : protein complex as shown by the supershift of the complex by the purified antiserum raised against OSBZ8p . Treatment of the seedlings with NaCl was found to enhance the complex formation , suggesting the regulation of OSBZ8 gene at both transcriptional and post-translational steps . Comparative EMSA with different varieties of rice suggests a positive correlation with the expression pattern of OSBZ8 and salt tolerance in rice cultivars .
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