Score: 9.00 |
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| | Author: Liu G Lu G Zeng L Wang GL .
| | Journal: Mol . Genet . Genomics Citation: V : 267 ( 4 ) P : 472-80 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12111554 Accession (PMID): 12111554 | | Abstract: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted .
These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 .
To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants .
Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates .
Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique .
Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers .
A high-resolution map of Pi9 ( t ) was constructed using BAC ends .
The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants .
These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region .
The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 3, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 4, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 5, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 6, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 7, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 8, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 9, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
[ Sen. 10, subscore: 1.00 ]: To understand the molecular basis of broad-spectrum resistance to rice blast , fine-scale mapping of the two blast resistance ( R ) genes , Pi9 ( t ) and Pi2 ( t ) , was conducted . These two genes were introgressed from different resistance donors , previously reported to confer resistance to many blast isolates in the Philippines , and were mapped to an approximately 10-cM interval on chromosome 6 . To further test their resistance spectrum , 43 blast isolates collected from 13 countries were used to inoculate the Pi2 ( t ) and Pi9 ( t ) plants . Pi9 ( t ) -bearing lines were highly resistant to all isolates tested , and lines carrying Pi2 ( t ) were resistant to 36 isolates , confirming the broad-spectrum resistance of these two genes to diverse blast isolates . Three RAPD markers tightly linked to Pi9 ( t ) were identified using the bulk segregant analysis technique . Twelve positive bacterial artificial chromosome ( BAC ) clones were identified and a BAC contig covering about 100 kb was constructed when the Pi9 ( t ) BAC library was screened with one of the markers . A high-resolution map of Pi9 ( t ) was constructed using BAC ends . The Pi2 ( t ) gene was tightly linked to all of the Pi9 ( t ) markers in 450 F ( 2 ) plants . These data suggest that Pi9 ( t ) and Pi2 ( t ) are either allelic or tightly linked in an approximately 100-kb region . The mapping results for Pi9 ( t ) and Pi2 ( t ) provide essential information for the positional cloning of these two important blast resistance genes in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| | Author: Lu G Jantasuriyarat C Zhou B Wang GL .
| | Journal: Theor . Appl . Genet . Citation: V : 108 ( 3 ) P : 525-34 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14605807 Accession (PMID): 14605807 | | Abstract: To identify early-induced defense genes involved in broad-spectrum resistance to rice blast , suppression subtractive hybridization was used to generate two cDNA libraries enriched for transcripts differentially expressed in Pi9 ( t ) -resistant and -susceptible plants .
After differential screening by membrane-based hybridization and subsequent confirmation by reverse Northern blot analysis , selected clones were sequenced and analyzed .
Forty-seven unique cDNA clones were found and assigned to eight different groups according to the putative function of their homologous genes in the database .
These genes may be involved in pathogen or stress response , signal transduction , transcription , cell transport , metabolism , energy or protein destination .
Northern blot analysis showed that most of these genes were induced or suppressed after blast infection , and that half of them showed differential expression patterns between compatible and incompatible interactions .
Interestingly , all but one of the identified genes are reported here for the first time to be involved in defense response to rice blast In addition , hybridization of these clones with cDNAs synthesized from RNA samples from bacterial blight-infected leaves showed that few of them are induced or repressed in Xa21 or Xa7-resistant plants , suggesting a minimum overlap of defense responses mediated by different resistance genes to fungal and bacterial pathogens at an early stage of infection .
Further characterization and functional analysis of these genes will enhance our understanding of the molecular mechanism of broad-spectrum resistance in rice . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To identify early-induced defense genes involved in broad-spectrum resistance to rice blast , suppression subtractive hybridization was used to generate two cDNA libraries enriched for transcripts differentially expressed in Pi9 ( t ) -resistant and -susceptible plants . After differential screening by membrane-based hybridization and subsequent confirmation by reverse Northern blot analysis , selected clones were sequenced and analyzed . Forty-seven unique cDNA clones were found and assigned to eight different groups according to the putative function of their homologous genes in the database . These genes may be involved in pathogen or stress response , signal transduction , transcription , cell transport , metabolism , energy or protein destination . Northern blot analysis showed that most of these genes were induced or suppressed after blast infection , and that half of them showed differential expression patterns between compatible and incompatible interactions . Interestingly , all but one of the identified genes are reported here for the first time to be involved in defense response to rice blast In addition , hybridization of these clones with cDNAs synthesized from RNA samples from bacterial blight-infected leaves showed that few of them are induced or repressed in Xa21 or Xa7-resistant plants , suggesting a minimum overlap of defense responses mediated by different resistance genes to fungal and bacterial pathogens at an early stage of infection . Further characterization and functional analysis of these genes will enhance our understanding of the molecular mechanism of broad-spectrum resistance in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| | Author: Deng Y Zhu X Shen Y He Z | | Journal: Theor . Appl . Genet . Citation: V : 113 ( 4 ) P : 705-13 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16832648 Accession (PMID): 16832648 | | Abstract: The identification and utilization of broad-spectrum resistance genes have been proven the most effective and economical approach to control rice blast disease .
To understand the molecular mechanism of broad-spectrum resistance to rice blast , we conducted genetic and fine mapping analysis of the blast resistance gene in a Chinese rice variety : Gumei 4 ( GM4 ) identified with broad-spectrum resistance and used in rice breeding for blast resistance for more than 20 years .
Genetic and mapping analysis indicated that blast resistance to nine isolates of different Chinese races in GM4 was controlled by the same dominant locus designated as Pigm ( t ) that was finely mapped to an approximately 70-kb interval between markers C5483 and C0428 on chromosome 6 , which contains five candidate NBS--LRR disease resistance genes .
The allelism test showed that Pigm ( t ) was either tightly linked or allelic to Pi2 and Pi9 , two known blast resistance genes .
Mapping information also indicated that another blast resistance gene Pi26 ( t ) might also be located at the same region .
Candidate genes were identified by sequence analysis of the Nipponbare and Pi9 locus and the corresponding region in GM4 .
Sequence divergence of candidate genes was observed between GM4 and model varieties Nipponbare and 9311 , and Pi9 .
Our current study provides essential information and new genetic resource for the cloning of functional resistance gene ( s ) and for marker-assisted selection in rice breeding for broad-spectrum blast resistance . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The identification and utilization of broad-spectrum resistance genes have been proven the most effective and economical approach to control rice blast disease . To understand the molecular mechanism of broad-spectrum resistance to rice blast , we conducted genetic and fine mapping analysis of the blast resistance gene in a Chinese rice variety : Gumei 4 ( GM4 ) identified with broad-spectrum resistance and used in rice breeding for blast resistance for more than 20 years . Genetic and mapping analysis indicated that blast resistance to nine isolates of different Chinese races in GM4 was controlled by the same dominant locus designated as Pigm ( t ) that was finely mapped to an approximately 70-kb interval between markers C5483 and C0428 on chromosome 6 , which contains five candidate NBS--LRR disease resistance genes . The allelism test showed that Pigm ( t ) was either tightly linked or allelic to Pi2 and Pi9 , two known blast resistance genes . Mapping information also indicated that another blast resistance gene Pi26 ( t ) might also be located at the same region . Candidate genes were identified by sequence analysis of the Nipponbare and Pi9 locus and the corresponding region in GM4 . Sequence divergence of candidate genes was observed between GM4 and model varieties Nipponbare and 9311 , and Pi9 . Our current study provides essential information and new genetic resource for the cloning of functional resistance gene ( s ) and for marker-assisted selection in rice breeding for broad-spectrum blast resistance .
[ Sen. 6, subscore: 1.00 ]: The identification and utilization of broad-spectrum resistance genes have been proven the most effective and economical approach to control rice blast disease . To understand the molecular mechanism of broad-spectrum resistance to rice blast , we conducted genetic and fine mapping analysis of the blast resistance gene in a Chinese rice variety : Gumei 4 ( GM4 ) identified with broad-spectrum resistance and used in rice breeding for blast resistance for more than 20 years . Genetic and mapping analysis indicated that blast resistance to nine isolates of different Chinese races in GM4 was controlled by the same dominant locus designated as Pigm ( t ) that was finely mapped to an approximately 70-kb interval between markers C5483 and C0428 on chromosome 6 , which contains five candidate NBS--LRR disease resistance genes . The allelism test showed that Pigm ( t ) was either tightly linked or allelic to Pi2 and Pi9 , two known blast resistance genes . Mapping information also indicated that another blast resistance gene Pi26 ( t ) might also be located at the same region . Candidate genes were identified by sequence analysis of the Nipponbare and Pi9 locus and the corresponding region in GM4 . Sequence divergence of candidate genes was observed between GM4 and model varieties Nipponbare and 9311 , and Pi9 . Our current study provides essential information and new genetic resource for the cloning of functional resistance gene ( s ) and for marker-assisted selection in rice breeding for broad-spectrum blast resistance .
[ Sen. 7, subscore: 1.00 ]: The identification and utilization of broad-spectrum resistance genes have been proven the most effective and economical approach to control rice blast disease . To understand the molecular mechanism of broad-spectrum resistance to rice blast , we conducted genetic and fine mapping analysis of the blast resistance gene in a Chinese rice variety : Gumei 4 ( GM4 ) identified with broad-spectrum resistance and used in rice breeding for blast resistance for more than 20 years . Genetic and mapping analysis indicated that blast resistance to nine isolates of different Chinese races in GM4 was controlled by the same dominant locus designated as Pigm ( t ) that was finely mapped to an approximately 70-kb interval between markers C5483 and C0428 on chromosome 6 , which contains five candidate NBS--LRR disease resistance genes . The allelism test showed that Pigm ( t ) was either tightly linked or allelic to Pi2 and Pi9 , two known blast resistance genes . Mapping information also indicated that another blast resistance gene Pi26 ( t ) might also be located at the same region . Candidate genes were identified by sequence analysis of the Nipponbare and Pi9 locus and the corresponding region in GM4 . Sequence divergence of candidate genes was observed between GM4 and model varieties Nipponbare and 9311 , and Pi9 . Our current study provides essential information and new genetic resource for the cloning of functional resistance gene ( s ) and for marker-assisted selection in rice breeding for broad-spectrum blast resistance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| | Author: Liu X Lin F Wang L Pan Q | | Journal: Genetics Citation: V : 176 P : Sep-41 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17507669 Accession (PMID): 17507669 | | Abstract: The indica rice variety Kasalath carries Pi36 , a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8 .
The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance ( R ) gene content of the interval and hence for the identification of candidate gene ( s ) for Pi36 .
Three such sequences , which all had both a nucleotide-binding site and a leucine-rich repeat motif , were present .
The three candidate genes were amplified from the genomic DNA of a number of varieties by long-range PCR , and the resulting amplicons were inserted into pCAMBIA1300 and/or pYLTAC27 vectors to determine sequence polymorphisms correlated to the resistance phenotype and to perform transgenic complementation tests .
Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063 , which allowed the identification of Pi36-3 as the functional gene , with the other two candidates being probable pseudogenes .
The Pi36-encoded protein is composed of 1056 amino acids , with a single substitution event ( Asp to Ser ) at residue 590 associated with the resistant phenotype .
Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
An RT-PCR analysis showed that Pi36 is constitutively expressed in Kasalath .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The indica rice variety Kasalath carries Pi36 , a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8 . The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance ( R ) gene content of the interval and hence for the identification of candidate gene ( s ) for Pi36 . Three such sequences , which all had both a nucleotide-binding site and a leucine-rich repeat motif , were present . The three candidate genes were amplified from the genomic DNA of a number of varieties by long-range PCR , and the resulting amplicons were inserted into pCAMBIA1300 and/or pYLTAC27 vectors to determine sequence polymorphisms correlated to the resistance phenotype and to perform transgenic complementation tests . Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063 , which allowed the identification of Pi36-3 as the functional gene , with the other two candidates being probable pseudogenes . The Pi36-encoded protein is composed of 1056 amino acids , with a single substitution event ( Asp to Ser ) at residue 590 associated with the resistant phenotype . Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t . An RT-PCR analysis showed that Pi36 is constitutively expressed in Kasalath .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
