| 56 matches found in 25 documents. Search time: 0.212 seconds. |
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| Title: The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes .
| | Author: Wang ZX Yano M Yamanouchi U Iwamoto M Monna L Hayasaka H Katayose Y Sasaki T | | Journal: Plant J Citation: V : 19 ( 1 ) P : 55-64 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10417726 Accession (PMID): 10417726 | | Abstract: Rice blast , caused by the fungal pathogen Magnaporthe grisea , is one of the most serious diseases of rice .
Here we describe the isolation and characterization of Pib , one of the rice blast resistance genes .
The Pib gene was isolated by a map-based cloning strategy .
The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site ( NBS ) and leucine-rich repeats ( LRRs ) ; thus , Pib is a member of the NBS-LRR class of plant disease resistance genes .
Interestingly , a duplication of the kinase 1a , 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein .
In addition , eight cysteine residues are clustered in the middle of the LRRs , a feature which has not been reported for other R genes .
Pib gene expression was induced upon altered environmental conditions , such as altered temperatures and darkness . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site ( NBS ) and leucine-rich repeats ( LRRs ) ; thus , Pib is a member of the NBS-LRR class of plant disease resistance genes .
[ Sen. 2, subscore: 1.00 ]: Here we describe the isolation and characterization of Pib , one of the rice blast resistance genes .
[ Sen. 3, subscore: 1.00 ]: The Pib gene was isolated by a map-based cloning strategy .
[ Sen. 5, subscore: 1.00 ]: Interestingly , a duplication of the kinase 1a , 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein .
[ Sen. 7, subscore: 1.00 ]: Pib gene expression was induced upon altered environmental conditions , such as altered temperatures and darkness .
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Score: 11.00 |
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| Title: Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences .
| | Author: Wang ZX Yamanouchi U Katayose Y Sasaki T Yano M | | Journal: Plant Mol . Biol . Citation: V : 47 ( 5 ) P : 653-61 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11725950 Accession (PMID): 11725950 | | Abstract: The rice blast resistance gene Pib is a member of the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) class of plant disease resistance ( R ) genes and belongs to a small gene family .
We describe here the isolation and characterization of a Pib homologue ( PibH8 ) , and extensive investigation of the expression of the Pib gene family ( Pib , PibH8 , HPibH8-1 , HPibH8-2 ) under various environmental and chemical treatments .
PibH8 shows 42% identity and 60% similarity to Pib and , like Pib , has a duplication of the kinase 1a , 2 , and 3a motifs of the NBS region in the N-terminal half of the protein .
Interestingly , genes of the Pib family exhibit a diurnal rhythm of expression .
RNA gel blot analysis revealed that their expression was regulated dramatically by environmental signals . such as temperature , light and water availability .
Their expression was also induced by chemical treatments , such as jasmonic acid , salicylic acid , ethylene and probenazole .
Our findings suggest that expression of the Pib gene family is up-regulated by environmental conditions that would favour pathogen infection .
This may reflect the evolution of anticipatory control of R gene expression . | Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: We describe here the isolation and characterization of a Pib homologue ( PibH8 ) , and extensive investigation of the expression of the Pib gene family ( Pib , PibH8 , HPibH8-1 , HPibH8-2 ) under various environmental and chemical treatments .
[ Sen. 3, subscore: 3.00 ]: PibH8 shows 42% identity and 60% similarity to Pib and , like Pib , has a duplication of the kinase 1a , 2 , and 3a motifs of the NBS region in the N-terminal half of the protein .
[ Sen. 1, subscore: 1.00 ]: The rice blast resistance gene Pib is a member of the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) class of plant disease resistance ( R ) genes and belongs to a small gene family .
[ Sen. 4, subscore: 1.00 ]: Interestingly , genes of the Pib family exhibit a diurnal rhythm of expression .
[ Sen. 7, subscore: 1.00 ]: Our findings suggest that expression of the Pib gene family is up-regulated by environmental conditions that would favour pathogen infection .
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Score: 1.00 |
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| Title: Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates .
| | Author: Wu JL Fan YY Li DB Zheng KL Leung H Zhuang JY .
| | Journal: Theor . Appl . Genet . Citation: V : 111 ( 1 ) P : 50-6 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15856160 Accession (PMID): 15856160 | | Abstract: To further our understanding of the genetic control of blast resistance in rice cultivar Gumei 2 and , consequently , to facilitate the utilization of this durably blast-resistant cultivar , we studied 304 recombinant inbred lines of indica rice cross Zhong 156/Gumei 2 and a linkage map comprising 181 markers .
An analysis of segregation for resistance against five isolates of rice blast suggested that one gene cluster and three additional major genes that are independently inherited are responsible for the complete resistance of Gumei 2 .
The gene cluster was located to chromosome 6 and includes two genes mapped previously , Pi25 ( t ) , against Chinese rice blast isolate 92-183 ( race ZC15 ) and Pi26 ( t ) against Philippine rice blast isolate Ca89 ( lineage 4 ) , and a gene for resistance against Philippine rice blast isolate 92330-5 ( lineage 17 ) .
Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
We used three components of partial blast resistance , percentage diseased leaf area ( DLA ) , lesion number and lesion size , all measured in the greenhouse , to measure the degree of susceptibility to isolates Ca89 and C923-39 and subsequently identified nine and eight quantitative trait loci ( QTLs ) , respectively .
Epistasis was determined to play an important role in partial resistance against Ca89 .
Using DLA measured on lines susceptible in a blast nursery , we detected six QTLs .
While different QTLs were detected for partial resistance to Ca89 and C923-39 , respectively , most were involved in the partial resistance in the field .
Our results suggest that the blast resistance in Gumei 2 is controlled by multiple major genes and minor genes with epistatic effects . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
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Score: 4.00 |
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| Title: Characterization of rice mutants with enhanced susceptibility to rice blast | | Author: Kim HK Lee SK Cho JI Lee S An G Jwa NS Kim BR Cho YC Han SS Bhoo SH Lee YH Hong YK Yi G Park DS Hahn TR Jeon JS .
| | Journal: Mol .
Cells Citation: V : 20 ( 3 ) P : 385-91 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16404154 Accession (PMID): 16404154 | | Abstract: As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance , we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 ( 91-033 ) from a T-DNA insertion library of the japonica rice cultivar , Hwayeong .
Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene ( s ) responsible for the enhanced susceptibility of the Hwayeong mutants .
A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar , Nagdong .
Chi-square analysis of the F2 segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy .
Randomly selected susceptible plants in the F2 population were used to build an initial map of Pi-hy .
The SSLP marker RM2265 on chromosome 2 was closely linked to resistance .
High resolution mapping using 105 F2 plants revealed that the resistance gene was tightly linked , or identical , to Pib , a resistance gene with a nucleotide binding sequence and leucine-rich repeats ( NB-LRR ) previously isolated .
Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene , demonstrating that the Pi-hy gene is Pib .
The Pib mutations in 1D-22-10-13 , 1D-54-16-8 , and 1C-143-16-1 were , respectively , a missense mutation in the conserved NB domain 3 , a nonsense mutation in the 5th LRR , and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus .
These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene .
They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene , demonstrating that the Pi-hy gene is Pib .
[ Sen. 7, subscore: 1.00 ]: High resolution mapping using 105 F2 plants revealed that the resistance gene was tightly linked , or identical , to Pib , a resistance gene with a nucleotide binding sequence and leucine-rich repeats ( NB-LRR ) previously isolated .
[ Sen. 9, subscore: 1.00 ]: The Pib mutations in 1D-22-10-13 , 1D-54-16-8 , and 1C-143-16-1 were , respectively , a missense mutation in the conserved NB domain 3 , a nonsense mutation in the 5th LRR , and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus .
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Score: 1.00 |
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| Title: Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes .
| | Author: Hayashi K Yoshida H Ashikawa I | | Journal: Citation: V : ( ) P : Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16673143 Accession (PMID): 16673143 | | Abstract: Blast resistance is one of the most important traits in rice breeding , and application of molecular markers for blast resistance breeding is likely to allow the rapid screening for the trait during early growth stages , without the need for inoculation of pathogen and phenotyping .
Allele-specific PCR markers and insertion/deletion ( InDel ) markers , which genotype single-nucleotide polymorphisms and InDel polymorphisms , respectively , are useful tools for marker-assisted selections .
We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib-which are commonly used in Japanese blast resistance rice breeding programs .
For each resistance gene , we used the segregation information from thousands of progeny in several crosses or published gene locations to generate a marker that cosegregated with the gene and markers that closely flanked the gene on either side .
The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
Therefore , these markers will likely facilitate the development of multiline cultivars carrying one or a combination of these nine blast resistance genes .
In addition , the systems we developed may be valuable tools in the quality control of seed production from blast-resistant multiline cultivars .
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[ Sen. 3, subscore: 1.00 ]: We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib-which are commonly used in Japanese blast resistance rice breeding programs .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
