| 22 matches found in 9 documents. Search time: 0.109 seconds. |
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| Title: Rice Triosephosphate Isomerase Gene 5 [ prime ] Sequence Directs [ beta ] -Glucuronidase Activity in Transgenic Tobacco but Requires an Intron for Expression in Rice .
| | Author: Xu Y Yu H Hall TC .
| | Journal: Citation: V : 106 ( 2 ) P : 459-467 Year: 1994 | | Literature: oryza Field: abstract Doc ID: pub12232342 Accession (PMID): 12232342 | | Abstract: In rice ( Oryza sativa L ) , cytosolic triosephosphate isomerase ( TPI ) is encoded by a single gene .
TPI catalyzes a vital step in glycolysis , and RNA blots showed that the tpi gene is expressed in all vegetative it issues ( root , culm , and leaves ) and in rice suspension cells .
No effect of light on expression was detected , but submergence of rice seedlings resulted in elevated levels of TPI mRNA in roots and culms .
The 2767-bp 5 [ prime ] upstream sequence of the tpi gene was fused translationally with the [ beta ] -glucuronidase ( gusA ) gene , and the resulting construct , TPI-GUS , was found to express constitutive , high levels of GUS activity in transgenic tobacco ( Nicotiana tabacum ) plants .
However , the same construct yielded no GUS activity in stably transformed rice plants , and RNA blots showed that no GUS mRNA could be detected even though stable integration of functional copies of the construct was confirmed by Southern blot and genomic polymerase chain reaction analyses .
Transient assays using particle bombardment yielded high levels of GUS expression from the TPI-GUS construct in tobacco leaves , but essentially no expression in rice , barley , or maize leaves .
When the first intron of the tpi gene was included in the construct ( TPI-int1-GUS ) , transient GUS activity was routinely obtained in rice leaves , revealing that the first intron of the rice tpi gene is crucial for its expression in rice .
TPI-int1-GUS also directed transient GUS expression in maize and barley leaves , but little or no activity was obtained from this construct in tobacco , tomato , or soybean leaves .
These results with the rice tpi promoter are in accordance with mounting evidence that differences in gene expression exist between monocots and dicots . | Matching Sentences:
[ Sen. 7, subscore: 3.00 ]: When the first intron of the tpi gene was included in the construct ( TPI-int1-GUS ) , transient GUS activity was routinely obtained in rice leaves , revealing that the first intron of the rice tpi gene is crucial for its expression in rice .
[ Sen. 2, subscore: 2.00 ]: TPI catalyzes a vital step in glycolysis , and RNA blots showed that the tpi gene is expressed in all vegetative it issues ( root , culm , and leaves ) and in rice suspension cells .
[ Sen. 4, subscore: 2.00 ]: The 2767-bp 5 [ prime ] upstream sequence of the tpi gene was fused translationally with the [ beta ] -glucuronidase ( gusA ) gene , and the resulting construct , TPI-GUS , was found to express constitutive , high levels of GUS activity in transgenic tobacco ( Nicotiana tabacum ) plants .
[ Sen. 1, subscore: 1.00 ]: In rice ( Oryza sativa L ) , cytosolic triosephosphate isomerase ( TPI ) is encoded by a single gene .
[ Sen. 3, subscore: 1.00 ]: No effect of light on expression was detected , but submergence of rice seedlings resulted in elevated levels of TPI mRNA in roots and culms .
[ Sen. 6, subscore: 1.00 ]: Transient assays using particle bombardment yielded high levels of GUS expression from the TPI-GUS construct in tobacco leaves , but essentially no expression in rice , barley , or maize leaves .
[ Sen. 8, subscore: 1.00 ]: TPI-int1-GUS also directed transient GUS expression in maize and barley leaves , but little or no activity was obtained from this construct in tobacco , tomato , or soybean leaves .
[ Sen. 9, subscore: 1.00 ]: These results with the rice tpi promoter are in accordance with mounting evidence that differences in gene expression exist between monocots and dicots .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: [ Phosphorus fractions under different land uses in Sanjiang plain ] | | Author: Qin SJ Liu JS Wang GP Zhou WM | | Journal: Huan Jing Ke Xue Citation: V : 28 P : 2777-82 Year: 2007 | | Literature: oryza Field: abstract Doc ID: pub18290436 Accession (PMID): 18290436 | | Abstract: Five land-uses type ( soybean-filed , rice-field , abandoned cultivation , artificial forest , natural wetland ) were obtained before planting and soil P fractions were determined by a modified Hedley fraction method .
The result showed that the content of total P ( TP ) in natural wetland was higher than other soil .
While the proportion of total inorganic P ( TPi ) in TP of farm land soil was higher than that of wetland .
Adversely , the proportion of total organic P ( TPo ) was higher in wetland and least in rice-field .
There was no obvious difference between that of abandoned field and artificial forest The values of labile inorganic phosphorus ( labile Pi ) under different land-uses were similar .
The contents of Resin-P and NaHCO3-Pi varied at a range of 32-36 . 3 mg x kg ( -1 ) and 33 . 77-50 . 42 mg x kg ( -1 ) , respectively .
The contents of NaOH-Pi and CHCl-Pi in farmland were higher than those of wetland , especially in rice-field which was 152 . 4 mg x kg ( -1 ) .
But for DHCl-Pi , the content was higher in wetland with the value of 84 . 3 mg x kg ( -1 ) , and the contents of NaOH-Pi , C HCl-Pi and DHCl-Pi in abandoned field and artificial forest were in the middle of the above two land use patterns , which indicated that the restoration of soil P in abandoned farmland tended to approach the level of natural wetland .
The value and percentage of Po was decreased greatly with the reclamation of wetland , and the decrease of C HCl-Po was the most significant in all forms of organic P , and its values in soybean-filed and rice-field were only 25 . 96% and 19 . 26% of that in wetland .
As the time went by the content of Po in abandoned arable land increased with very slow speed , which indicated that the restoration of wetland after reclamation would need a long time .
The distribution of soil P in different P fractions has significant difference for the land-use change in Sanjiang plain .
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[ Sen. 3, subscore: 1.00 ]: While the proportion of total inorganic P ( TPi ) in TP of farm land soil was higher than that of wetland .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Changes in protein expression profiles between a low phytic acid rice ( Oryza sativa L Ssp . japonica ) line and its parental line : a proteomic and bioinformatic approach .
| | Author: Emami K Morris NJ Cockell SJ Golebiowska G Shu QY Gatehouse AM | | Journal: J Agric Food Chem Citation: V : 58 P : 6912-22 Year: 2010 | | Literature: oryza Field: abstract Doc ID: pub20441221 Accession (PMID): 20441221 | | Abstract: The seed proteome of a low phytic acid ( lpa ) rice line ( Os-lpa-XS110-1 ) , developed as a novel food source , was compared to that of its parental line , Xiushui 110 ( XS-110 ) .
Analysis by surfaced enhanced laser desorption ionization-time-of-flight mass spectrometry ( SELDI-TOF MS ) and two-dimensional gel electrophoresis ( 2-DE ) allowed the detection of a potential low molecular weight biomarker and identification of 23 differentially expressed proteins that include stress-related proteins , storage proteins , and potential allergens .
Bioinformatic analyses revealed that triose phosphate isomerase ( TPI ) and fructose bisphosphatealdolase ( FBA ) , two major differentially expressed proteins , are involved in myo-inositol metabolism .
Accumulation of globulin was also significantly decreased in the lpa line .
This study demonstrates the potential of proteomic and bioinformatic profiling techniques for safety assessment of novel foods .
Furthermore , these techniques provide powerful tools for studying functional genomics due to the possibility of identifying genes related to the mutated traits .
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[ Sen. 3, subscore: 1.00 ]: Bioinformatic analyses revealed that triose phosphate isomerase ( TPI ) and fructose bisphosphatealdolase ( FBA ) , two major differentially expressed proteins , are involved in myo-inositol metabolism .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae .
| | Author: Kim IS Kim YS Yoon HS | | Journal: Mol Cells Citation: V : 33 P : 285-93 Year: 2012 | | Literature: oryza Field: abstract Doc ID: pub22382682 Accession (PMID): 22382682 | | Abstract: Abscisic acid stress ripening ( ASR1 ) protein is a small hydrophilic , low molecular weight , and stress-specific plant protein .
The gene coding region of ASR1 protein , which is induced under high salinity in rice ( Oryza sativa Ilmi ) , was cloned into a yeast expression vector pVTU260 and transformed into yeast cells .
Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide ( H ( 2 ) O ( 2 ) ) , high salinity ( NaCl ) , heat shock , menadione , copper sulfate , sulfuric acid , lactic acid , salicylic acid , and also high concentration of ethanol .
In particular , the expression of metabolic enzymes ( Fba1p , Pgk1p , Eno2p , Tpi1p , and Adh1p ) , antioxidant enzyme ( Ahp1p ) , molecular chaperone ( Ssb1p ) , and pyrimidine biosynthesis-related enzyme ( Ura1p ) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells .
All of these enzymes contribute to an alleviated redox state to H2O2-induced oxidative stress .
In the in vitro assay , the purified ASR1 protein was able to scavenge ROS by converting H ( 2 ) O ( 2 ) to H ( 2 ) O Taken together , these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells .
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[ Sen. 4, subscore: 1.00 ]: In particular , the expression of metabolic enzymes ( Fba1p , Pgk1p , Eno2p , Tpi1p , and Adh1p ) , antioxidant enzyme ( Ahp1p ) , molecular chaperone ( Ssb1p ) , and pyrimidine biosynthesis-related enzyme ( Ura1p ) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Changes in the contents of metabolites and enzyme activities in rice plants responding to Rhizoctonia solani Kuhn infection : activation of glycolysis and connection to phenylpropanoid pathway .
| | Author: Mutuku JM Nose A | | Journal: Plant Cell Physiol Citation: V : 53 P : 1017-32 Year: 2012 | | Literature: oryza Field: abstract Doc ID: pub22492233 Accession (PMID): 22492233 | | Abstract: Rhizoctonia solani Kuhn causes sheath blight disease in rice , and genetic resistance against it is the most desirable characteristic .
Current improvement efforts are based on analysis of polygenic quantitative trait loci ( QTLs ) , but interpretation is limited by the lack of information on the changes in metabolic pathways .
Our previous studies linked activation of the glycolytic pathway to enhanced generation of lignin in the phenylpropanoid pathway .
The current studies investigated the regulation of glycolysis by examining the time course of changes in enzymatic activities and metabolite contents .
The results showed that the activities of all glycolytic enzymes as well as fructose-6-phosphate ( F-6-P ) , fructose-1 , 6-bisphosphate ( F-1 , 6-P ( 2 ) ) , dihydroxyacetone phosphate ( DHAP ) , glyceraldehyde-3-phosphate ( GAP ) , 3-phosphoglycerate ( 3-PG ) , phosphoenolpyruvate ( PEP ) and pyruvate contents increased .
These results combined with our previous findings that the expression of phosphoglucomutase ( PGM ) , triosephosphate isomerase ( TPI ) , glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) , enolase and pyruvate kinase ( PK ) increased after infection suggested that the additional establishment of glycolysis in the cytosol compartment occurred after infection .
Further evidence for this was our recent findings that the increase in expression of the 6-phosphofructokinase ( PFK ) plastid isozyme Os06g05860 was accompanied by an increase in expression of three cytosolic PFK isozymes , ie Os01g09570 , Os01g53680 and Os04g39420 , as well as pyrophosphate-dependent phosphofrucokinase ( PFP ) isozymes Os08g25720 ( alpha-subunit ) and Os06g13810 ( beta-subunit ) in infected rice plants of the resistant line .
The results also showed that the reactions catalysed by PFK/PFP , aldolase , GAPDH + phosphoglycerate kinase ( PGK ) and PK in leaf sheaths of R solani-infected rice plants were non-equilibrium reactions in vivo .
This study showed that PGM , phosphoglucose isomerase ( PGI ) , TPI and phosphoglycerate mutase ( PGmu ) + enolase could be regulated through coarse control whereas , PFK/PFP , aldolase , GAPDH + PGK and PK could be regulated through coarse and fine controls simultaneously .
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[ Sen. 6, subscore: 1.00 ]: These results combined with our previous findings that the expression of phosphoglucomutase ( PGM ) , triosephosphate isomerase ( TPI ) , glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) , enolase and pyruvate kinase ( PK ) increased after infection suggested that the additional establishment of glycolysis in the cytosol compartment occurred after infection .
[ Sen. 9, subscore: 1.00 ]: This study showed that PGM , phosphoglucose isomerase ( PGI ) , TPI and phosphoglycerate mutase ( PGmu ) + enolase could be regulated through coarse control whereas , PFK/PFP , aldolase , GAPDH + PGK and PK could be regulated through coarse and fine controls simultaneously .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
