| 73 matches found in 35 documents. Search time: 0.062 seconds. |
|---|
|
Score: 2.00 |
|---|
| | Author: L HJ Shao CG Gong ZX .
| | Journal: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Citation: V : 34 ( 5 ) P : 565-70 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12198557 Accession (PMID): 12198557 | | Abstract: Rice ragged stunt oryzavirus ( RRSV ) is a member of the genus oryzavirus within the family Reoviridae .
Its genome consists of ten segments of dsRNA .
The functions of all products encoded by these viral genome segments , except one encoded by S9 , have not yet been elucidated .
In the present study , the ORF of S 8 of RRSV-Philippines isolate was sequenced and expressed in E coli .
The 67 kD product of S8 could be self-cleaved into two fragments with molecular weights of 43 kD and 26 kD .
Western blotting indicated that both 67 kD and 43 kD products were major structural proteins of the virus .
It was also found that the 67 kD protein could self aggregate into aggregates with higher sedimentation rate in sucrose gradients during centrifugation .
Moreover , the self-aggregation process could be accelerated by the complex of S6 product and genome dsRNAs of RRSV .
These results suggest that the S8 products , 67 kD or 43 kD , may be the structural components of the viral inner-capsid . | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: The 67 kD product of S8 could be self-cleaved into two fragments with molecular weights of 43 kD and 26 kD .
[ Sen. 9, subscore: 1.00 ]: These results suggest that the S8 products , 67 kD or 43 kD , may be the structural components of the viral inner-capsid .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| | Author: Distfano AJ Conci LR Muoz Hidalgo M Guzmn FA Hopp HE del Vas M | | Journal: Arch . Virol . Citation: V : 147 ( 9 ) P : 1699-709 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12209310 Accession (PMID): 12209310 | | Abstract: This is the first sequence-based characterization of Mal de R Cuarto virus ( MRCV ) , currently classified as a variant of Maize rough dwarf virus ( MRDV ) and exclusively found in South America .
We sequenced and analyzed genome segments S4 and S8 .
MRCV S4 coded for a putative 131 . 67 kDa protein while MRCV S8 coded for a putative 68 . 26 kDa protein containing an ATP/GTP-binding motif .
The 5 and 3 ends of MRCV segments , were 5AAGUUUUU3 and 5CAGCUnnnGUC3 , respectively .
Prediction of secondary structure of both segments coding strands showed that terminal regions were able to form structures that are proposed to be replication and packaging signals .
MRCV S4 showed identity to members of Fijivirus as well as to two other genera of the Reoviridae family .
MRCV S8 revealed identity with Rice black streaked dwarf virus ( RBSDV ) S8 , MRDV S7 , Oat sterile dwarf virus ( OSDV ) S9 and Nilaparvata lugens reovirus ( NLRV ) S7 .
While MRDV and RBSDV segments are highly homologous between each other , MRCV identity levels with them was considerably lower .
We discussed the evolutionary relationships of MRCV to other Reoviridae , and based on phylogenetic analysis we proposed that although MRCV is related to MRDV , it could be regarded as a new species of the Fijivirus genus .
| Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: MRCV S8 revealed identity with Rice black streaked dwarf virus ( RBSDV ) S8 , MRDV S7 , Oat sterile dwarf virus ( OSDV ) S9 and Nilaparvata lugens reovirus ( NLRV ) S7 .
[ Sen. 2, subscore: 1.00 ]: We sequenced and analyzed genome segments S4 and S8 .
[ Sen. 3, subscore: 1.00 ]: MRCV S4 coded for a putative 131 . 67 kDa protein while MRCV S8 coded for a putative 68 . 26 kDa protein containing an ATP/GTP-binding motif .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| | Author: Deng W Yang X Zhang Y Liu Y Kang L | | Journal: Wei Sheng Wu Xue Bao Citation: V : 40 ( 5 ) P : 488-94 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12548759 Accession (PMID): 12548759 | | Abstract: The double strand RNA of maize rough dwarf virus ( MRDV ) was prepared from the maize samples showing symptoms which was from the Luanchen county of Heibei province of China .
The primers were designed according to the known sequence of MRDV , the cDNA sequence of the seventh segment of MRDV was obtained by RT-PCR , the S7 sequence was analyzed by computer after sequencing .
The results showed : the full length of the S7 cDNA is 1936 bp and equal to that of the S7 cDNA from abroad , the two open reading frame ( ORF1 and ORF2 ) contained in the S7 segment are also unchanged .
In comparison with the S7 segment from Italy , the homology of S7 nucleotide is 87 . 7% and the homology of ORF1 amino acid sequence is 91 . 6% .
However , the MRDV S7 segment and the rice black strike dwarf virus S8 segment showed 95 . 5% nucleotide identities and 93 . 5% ORF1 amino acid identities .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: However , the MRDV S7 segment and the rice black strike dwarf virus S8 segment showed 95 . 5% nucleotide identities and 93 . 5% ORF1 amino acid identities .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| | Author: Li S Liu H Chen Z Li Y | | Journal: Wei Sheng Wu Xue Bao Citation: V : 41 ( 2 ) P : 162-6 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12549020 Accession (PMID): 12549020 | | Abstract: Outer coat protein gene ( S8 ) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8 .
The recombinant vector pVL1393-S8 and the linear baculovirus RP23 .
LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8 .
RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot .
The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Outer coat protein gene ( S8 ) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8 .
[ Sen. 5, subscore: 1.00 ]: The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| | Author: Beers EP Jones AM Dickerman AW .
| | Journal: Phytochemistry Citation: V : 65 ( 1 ) P : 43-58 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14697270 Accession (PMID): 14697270 | | Abstract: The Arabidopsis thaliana genome has over 550 protease sequences representing all five catalytic types : serine , cysteine , aspartic acid , metallo and threonine ( MEROPS peptidase database , http : //merops . sanger . ac . uk/ ) , which probably reflect a wide variety of as yet unidentified functions performed by plant proteases .
Recent indications that the 26S proteasome , a T1 family-threonine protease , is a regulator of light and hormone responsive signal transduction highlight the potential of proteases to participate in many aspects of plant growth and development .
Recent discoveries that proteases are required for stomatal distribution , embryo development and disease resistance point to wider roles for four additional multigene families that include some of the most frequently studied ( yet poorly understood ) plant proteases : the subtilisin-like , serine proteases ( family S8 ) , the papain-like , cysteine proteases ( family C1A ) , the pepsin-like , aspartic proteases ( family A1 ) and the plant matrixin , metalloproteases ( family M10A ) .
In this report , 54 subtilisin-like , 30 papain-like and 59 pepsin-like proteases from Arabidopsis , are compared with S8 , C1A and A1 proteases known from other plant species at the functional , phylogenetic and gene structure levels .
Examples of structural conservation between S8 , C1A and A1 genes from rice , barley , tomato and soybean and those from Arabidopsis are noted , indicating that some common , essential plant protease roles were established before the divergence of monocots and eudicots .
Numerous examples of tandem duplications of protease genes and evidence for a variety of restricted expression patterns suggest that a high degree of specialization exists among proteases within each family .
We propose that comprehensive analysis of the functions of these genes in Arabidopsis will firmly establish serine , cysteine and aspartic proteases as regulators and effectors of a wide range of plant processes .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Recent discoveries that proteases are required for stomatal distribution , embryo development and disease resistance point to wider roles for four additional multigene families that include some of the most frequently studied ( yet poorly understood ) plant proteases : the subtilisin-like , serine proteases ( family S8 ) , the papain-like , cysteine proteases ( family C1A ) , the pepsin-like , aspartic proteases ( family A1 ) and the plant matrixin , metalloproteases ( family M10A ) .
[ Sen. 4, subscore: 1.00 ]: In this report , 54 subtilisin-like , 30 papain-like and 59 pepsin-like proteases from Arabidopsis , are compared with S8 , C1A and A1 proteases known from other plant species at the functional , phylogenetic and gene structure levels .
[ Sen. 5, subscore: 1.00 ]: Examples of structural conservation between S8 , C1A and A1 genes from rice , barley , tomato and soybean and those from Arabidopsis are noted , indicating that some common , essential plant protease roles were established before the divergence of monocots and eudicots .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
