| 27 matches found in 9 documents. Search time: 0.004 seconds. |
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| | Author: Lee DY Lee J Moon S Park SY An G | | Journal: Plant J Citation: V : 49 ( 1 ) P : 64-78 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17144896 Accession (PMID): 17144896 | | Abstract: Regulating the transition of meristem identity is a critical step in reproductive development .
After the shoot apical meristem ( SAM ) acquires inflorescence meristem identity , it goes through a sequential transition to second and higher-order meristems that can eventually give rise to floral organs .
Despite ample information on the molecular mechanisms that control the transition from SAM to inflorescence meristems , little is known about the mechanism for inflorescence development , especially in monocots .
Here , we report the identification of the SUPERNUMERARY BRACT ( SNB ) gene controlling the transition from spikelet meristem to floral meristem and the floral organ development .
This gene encodes a putative transcription factor carrying two AP2 domains .
The SNB : GFP fusion protein is localized to the nucleus .
SNB is expressed in all the examined it issues , but most strongly in the newly emerging spikelet meristems .
In SNB knockout plants , the transition from spikelet meristems to floral meristems is delayed , resulting in the production of multiple rudimentary glumes in an alternative phyllotaxy .
The development of additional bracts interferes with subsequent floral architecture .
In some spikelets , the empty glumes and lodicules are transformed into lemma/palea-like organs .
Occasionally , the number of stamens and carpels is altered and an ectopic floret occurs in the axil of the rachilla .
These phenotypes suggest that snb is a heterochronic mutant , affecting the phase transition of spikelet meristems , the pattern formation of floral organs and spikelet meristem determinancy .
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[ Sen. 4, subscore: 1.00 ]: Here , we report the identification of the SUPERNUMERARY BRACT ( SNB ) gene controlling the transition from spikelet meristem to floral meristem and the floral organ development .
[ Sen. 6, subscore: 1.00 ]: The SNB : GFP fusion protein is localized to the nucleus .
[ Sen. 7, subscore: 1.00 ]: SNB is expressed in all the examined it issues , but most strongly in the newly emerging spikelet meristems .
[ Sen. 8, subscore: 1.00 ]: In SNB knockout plants , the transition from spikelet meristems to floral meristems is delayed , resulting in the production of multiple rudimentary glumes in an alternative phyllotaxy .
[ Sen. 12, subscore: 1.00 ]: These phenotypes suggest that snb is a heterochronic mutant , affecting the phase transition of spikelet meristems , the pattern formation of floral organs and spikelet meristem determinancy .
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Score: 3.00 |
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| | Author: Park DS Lee SK Lee JH Song MY Song SY Kwak DY Yeo US Jeon NS Park SK Yi G Song YC Nam MH Ku YC Jeon JS | | Journal: Theor Appl Genet Citation: V : 115 P : 537-47 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17585380 Accession (PMID): 17585380 | | Abstract: The development of rice varieties ( Oryza sativa L ) that are resistant to the brown planthopper ( BPH ; Nilaparvata lugens Stal ) is an important objective in current breeding programs .
In this study , we generated 132 BC ( 5 ) F ( 5 ) near-isogenic rice lines ( NILs ) by five backcrosses of Samgangbyeo , a BPH resistant indica variety carrying the Bph1 locus , with Nagdongbyeo , a BPH susceptible japonica variety .
To identify genes that confer BPH resistance , we employed representational difference analysis ( RDA ) to detect transcripts that were exclusively expressed in one of our BPH resistant NIL , SNBC61 , during insect feeding .
The chromosomal mapping of the RDA clones that we subsequently isolated revealed that they are located in close proximity either to known quantitative trait loci or to an introgressed SSR marker from the BPH resistant donor parent Samgangbyeo .
Genomic DNA gel-blot analysis further revealed that loci of all RDA clones in SNBC61 correspond to the alleles of Samgangbyeo .
Most of the RDA clones were found to be exclusively expressed in SNBC61 and could be assigned to functional groups involved in plant defense .
These RDA clones therefore represent candidate defense genes for BPH resistance .
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[ Sen. 3, subscore: 1.00 ]: To identify genes that confer BPH resistance , we employed representational difference analysis ( RDA ) to detect transcripts that were exclusively expressed in one of our BPH resistant NIL , SNBC61 , during insect feeding .
[ Sen. 5, subscore: 1.00 ]: Genomic DNA gel-blot analysis further revealed that loci of all RDA clones in SNBC61 correspond to the alleles of Samgangbyeo .
[ Sen. 6, subscore: 1.00 ]: Most of the RDA clones were found to be exclusively expressed in SNBC61 and could be assigned to functional groups involved in plant defense .
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Score: 4.00 |
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| | Author: Park DS Song MY Park SK Lee SK Lee JH Song SY Eun MY Hahn TR Sohn JK Yi G Nam MH Jeon JS | | Journal: Mol Genet Genomics Citation: V : 280 P : 163-72 Year: 2008 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub18553105 Accession (PMID): 18553105 | | Abstract: During brown planthopper ( BPH ) feeding on rice plants , we employed a modified representational difference analysis ( RDA ) method to detect rare transcripts among those differentially expressed in SNBC61 , a BPH resistant near-isogenic line ( NIL ) carrying the Bph1 resistance gene .
This identified 3 RDA clones : OsBphi237 , OsBphi252 and OsBphi262 .
DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo .
Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding .
Interestingly , analysis of 64 SNBC NILs , derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo , using a cleaved amplified polymorphic sequence ( CAPS ) marker indicated that OsBphi252 , which encodes a putative lipoxygenase ( LOX ) , co-segregates with BPH resistance .
Our results suggest that OsBphi252 is tightly linked to Bph1 , and may be useful in marker-assisted selection ( MAS ) for resistance to BPH .
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[ Sen. 1, subscore: 1.00 ]: During brown planthopper ( BPH ) feeding on rice plants , we employed a modified representational difference analysis ( RDA ) method to detect rare transcripts among those differentially expressed in SNBC61 , a BPH resistant near-isogenic line ( NIL ) carrying the Bph1 resistance gene .
[ Sen. 3, subscore: 1.00 ]: DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo .
[ Sen. 4, subscore: 1.00 ]: Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding .
[ Sen. 5, subscore: 1.00 ]: Interestingly , analysis of 64 SNBC NILs , derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo , using a cleaved amplified polymorphic sequence ( CAPS ) marker indicated that OsBphi252 , which encodes a putative lipoxygenase ( LOX ) , co-segregates with BPH resistance .
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Score: 6.00 |
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| | Author: Zhu QH Upadhyaya NM Gubler F Helliwell CA | | Journal: BMC Plant Biol Citation: V : 9 P : 149 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub20017947 Accession (PMID): 20017947 | | Abstract: BACKGROUND : Regulation of gene expression by microRNAs ( miRNAs ) plays a crucial role in many developmental and physiological processes in plants . miRNAs act to repress expression of their target genes via mRNA cleavage or translational repression .
Dozens of miRNA families have been identified in rice , 21 of which are conserved between rice and Arabidopsis . miR172 is a conserved miRNA family which has been shown to regulate expression of APETALA2 ( AP2 ) -like transcription factors in Arabidopsis and maize .
The rice genome encodes five AP2-like genes predicted to be targets of miR172 .
To determine whether these rice AP2-like genes are regulated by miR172 and investigate the function of the target genes , we studied the effect of over-expressing two members of the miR172 family on rice plant development .
RESULTS : Analysis of miR172 expression showed that it is most highly expressed in late vegetative stages and developing panicles .
Analyses of expression of three miR172 targets showed that SUPERNUMERARY BRACT ( SNB ) and Os03g60430 have high expression in developing panicles .
Expression of miR172 was not inversely correlated with expression of its targets although miR172-mediated cleavage of SNB was detected by 5 rapid amplification of cDNA ends ( RACE ) .
Over-expression of miR172b in rice delayed the transition from spikelet meristem to floral meristem , and resulted in floral and seed developmental defects , including changes to the number and identity of floral organs , lower fertility and reduced seed weight .
Plants over-expressing miR172b not only phenocopied the T-DNA insertion mutant of SNB but showed additional defects in floret development not seen in the snb mutant .
However SNB expression was not reduced in the miR172b over-expression plants .
CONCLUSIONS : The phenotypes resulting from over-expression of miR172b suggests it represses SNB and at least one of the other miR172 targets , most likely Os03g60430 , indicating roles for other AP2-like genes in rice floret development . miR172 and the AP2-like genes had overlapping expression patterns in rice and their expression did not show an obvious negative correlation .
There was not a uniform decrease in the expression of the AP2-like miR172 target mRNAs in the miR172b over-expression plants .
These observations are consistent with miR172 functioning via translational repression or with expression of the AP2-like genes being regulated by a negative feedback loop .
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[ Sen. 9, subscore: 2.00 ]: Plants over-expressing miR172b not only phenocopied the T-DNA insertion mutant of SNB but showed additional defects in floret development not seen in the snb mutant .
[ Sen. 6, subscore: 1.00 ]: Analyses of expression of three miR172 targets showed that SUPERNUMERARY BRACT ( SNB ) and Os03g60430 have high expression in developing panicles .
[ Sen. 7, subscore: 1.00 ]: Expression of miR172 was not inversely correlated with expression of its targets although miR172-mediated cleavage of SNB was detected by 5 rapid amplification of cDNA ends ( RACE ) .
[ Sen. 10, subscore: 1.00 ]: However SNB expression was not reduced in the miR172b over-expression plants .
[ Sen. 11, subscore: 1.00 ]: CONCLUSIONS : The phenotypes resulting from over-expression of miR172b suggests it represses SNB and at least one of the other miR172 targets , most likely Os03g60430 , indicating roles for other AP2-like genes in rice floret development . miR172 and the AP2-like genes had overlapping expression patterns in rice and their expression did not show an obvious negative correlation .
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Score: 1.00 |
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| | Author: Zhang Z Friesen TL Xu SS Shi G Liu Z Rasmussen JB Faris JD | | Journal: Plant J Citation: V : 65 P : 27-38 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21175887 Accession (PMID): 21175887 | | Abstract: The pathogen Stagonospora nodorum produces multiple effectors , also known as host-selective toxins ( HSTs ) , that interact with corresponding host sensitivity genes in an inverse gene-for-gene manner to cause the disease Stagonospora nodorum blotch ( SNB ) in wheat .
In this study , a sensitivity gene was identified in Aegilops tauschii , the diploid D-genome donor of common wheat .
The gene was mapped to the short arm of chromosome 5D and mediated recognition of the effector SnTox3 , which was previously shown to be recognized by the wheat gene Snn3 on chromosome arm 5BS .
Comparative mapping suggested that Snn3 and the gene on 5DS are probably homoeologous and derived from a common ancestor .
Therefore , we propose to designate these genes as Snn3-B1 and Snn3-D1 , respectively .
Compatible Snn3-D1-SnTox3 interactions resulted in more severe necrosis in both effector infiltration and spore inoculation experiments than compatible Snn3-B1-SnTox3 interactions , indicating that Snn3-B1 and Snn3-D1 may have different affinities in SnTox3 recognition or signal transduction .
Wheat bin-mapped expressed sequence tags and good levels of collinearity among the wheat Snn3 regions , rice ( Oryza sativa ) , and Brachypodium distachyon were exploited for saturation and fine mapping of the Snn3-D1 locus .
Markers delineating the Snn3-D1 locus to a 1 . 4 cM interval will be useful for initiating positional cloning .
Further characterization of how these homoeologous genes mediate recognition of the same pathogen effector should enhance understanding of host manipulation by necrotrophic pathogens in causing disease .
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[ Sen. 1, subscore: 1.00 ]: The pathogen Stagonospora nodorum produces multiple effectors , also known as host-selective toxins ( HSTs ) , that interact with corresponding host sensitivity genes in an inverse gene-for-gene manner to cause the disease Stagonospora nodorum blotch ( SNB ) in wheat .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
