| 16 matches found in 7 documents. Search time: 0.111 seconds. |
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| Title: Synthesis of hemoglobins in rice ( Oryza sativa var .
Jackson ) plants growing in normal and stress conditions .
| | Author: Lira-Ruan V Sarath G Klucas RV Arredondo-Peter R | | Journal: Citation: V : 161 ( 2 ) P : 279-287 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11448759 Accession (PMID): 11448759 | | Abstract: In rice ( Oryza sativa var .
Jackson ) at least three copies of hemoglobin ( hb ) gene exist Rice hb1 and hb2 genes are differentially expressed in roots and leaves from mature plants .
We used polyclonal antibodies raised to recombinant rice Hb1 and Western blotting to analyze the synthesis of Hbs in rice plants growing under normal or stress conditions .
Results showed that rice Hbs are synthesized in coleoptiles , seminal roots and embryos from seeds germinated for 6 days , and also in leaves and roots from plants 2-14 weeks after germination .
Analysis of Hb synthesis in stressed rice showed that : ( i ) level of Hbs was higher in etiolated than control plants , ( ii ) level of Hbs increased in roots from flooded rice , and ( iii ) level of Hbs did not change under oxidative ( H ( 2 ) O ( 2 ) ) , nitrosative ( SNP ) and hormonal ( 2 , 4-D ) stresses .
These results suggest that the effect of light withdrawal in etiolated leaves and O ( 2 ) -limiting conditions in flooded roots , but not oxidative , nitrosative and hormonal stresses , modulate the synthesis of rice Hbs . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Jackson ) at least three copies of hemoglobin ( hb ) gene exist Rice hb1 and hb2 genes are differentially expressed in roots and leaves from mature plants .
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| Title: Use of in silico ( computer ) methods to predict and analyze the tertiary structure of plant hemoglobins .
| | Author: Gopalasubramaniam SK Garrocho-Villegas V Rivera GB Pastor N Arredondo-Peter R | | Journal: Methods Enzymol Citation: V : 436 P : 393-410 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18237645 Accession (PMID): 18237645 | | Abstract: Amino acid sequences for more than 60 plant hemoglobins ( Hbs ) are deposited in databases , but the tertiary structure of only 4 plant Hbs have been reported ; thus , the gap between the reported sequences and structures of plant Hbs is large .
Elucidating the structure of plant Hbs is essential to fully understanding the function of these proteins in plant cells .
Determining the actual protein structure by experimental methods ( ie , by X-ray crystallography ) requires considerable protein material and is expensive ; thus , this type of work is limited to few laboratories around the world .
In silico ( computer ) methods to predict the tertiary structure of proteins from amino acid sequences have been implemented and are helping reduce the sequence-structure gap .
Thus , in silico methods are useful tools for predicting the tertiary structure of several plant Hbs from amino acid sequences deposited in databases .
In this chapter , we describe a method for predicting and analyzing the structure of a rice Hb2 from the template structure of native rice Hb1 .
This method is based on a comparative modeling method that uses programs from the SWISS-MODEL server .
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[ Sen. 6, subscore: 1.00 ]: In this chapter , we describe a method for predicting and analyzing the structure of a rice Hb2 from the template structure of native rice Hb1 .
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| Title: Expression of non-symbiotic hemoglobin 1 and 2 genes in rice ( Oryza sativa ) embryonic organs .
| | Author: Lira-Ruan V Ruiz-Kubli M Arredondo-Peter R | | Journal: Commun Integr Biol Citation: V : 4 P : 457-8 Year: 2011 Type: PubMed-not-MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21966570 Accession (PMID): 21966570 | | Abstract: Rice ( Oryza sativa ) contains five copies of the non-symbiotic hemoglobin ( hb ) gene , namely hb1 to hb5 .
Previous analysis by RT-PCR revealed that rice hb1 expresses in roots and leaves and hb2 expresses in leaves .
However , it is not known whether or not hb1 and hb2 express in rice embryonic organs .
Here , we report the expression of hb1 and hb2 genes in rice embryonic organs using RT-PCR and specific oligos for Hb1 and Hb2 .
Our results indicate that hb1 and hb2 genes express in embryonic organs in rice growing under normal conditions .
Specifically , hb1 expresses in rice embryos and seminal roots , and hb2 expresses in embryos , coleoptiles and seminal roots .
These observations suggest that Hb1 and Hb2 coexist and function in rice embryonic organs .
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[ Sen. 4, subscore: 2.00 ]: Here , we report the expression of hb1 and hb2 genes in rice embryonic organs using RT-PCR and specific oligos for Hb1 and Hb2 .
[ Sen. 2, subscore: 1.00 ]: Previous analysis by RT-PCR revealed that rice hb1 expresses in roots and leaves and hb2 expresses in leaves .
[ Sen. 3, subscore: 1.00 ]: However , it is not known whether or not hb1 and hb2 express in rice embryonic organs .
[ Sen. 5, subscore: 1.00 ]: Our results indicate that hb1 and hb2 genes express in embryonic organs in rice growing under normal conditions .
[ Sen. 6, subscore: 1.00 ]: Specifically , hb1 expresses in rice embryos and seminal roots , and hb2 expresses in embryos , coleoptiles and seminal roots .
[ Sen. 7, subscore: 1.00 ]: These observations suggest that Hb1 and Hb2 coexist and function in rice embryonic organs .
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| Title: Fine mapping of a major QTL for flag leaf width in rice , qFLW4 , which might be caused by alternative splicing of NAL1 .
| | Author: Chen M Luo J Shao G Wei X Tang S Sheng Z Song J Hu P | | Journal: Plant Cell Rep Citation: V : 31 P : 863-72 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22179305 Accession (PMID): 22179305 | | Abstract: Leaf width is an important agricultural trait in rice .
QTL mapping in a recombinant inbred line population derived from the cross between the javanica cultivar D50 ( narrow-leaved ) and the indica cultivar HB277 ( wide-leaved ) identified five QTLs controlling flag leaf width .
Fine mapping of the major QTL qFLW4 narrowed its location to a 74 . 8 kb interval between the SSR loci RM17483 and RM17486 , a region which also contains the gene NAL1 ( Narrow leaf 1 ) .
There was no difference in the level of NAL1 expression between cvs D50 and HB277 , but an analysis of the NAL1 transcripts showed that while most ( if not all ) of those produced in cv .
D50 were full-length , two-thirds of those in HB277 were non-functional due to either loss or gain of sequence .
The inference was that NAL1 is probably synonymous with qFLW4 , and that the functional difference between the two alleles was due to alternative splicing .
The analysis of expression of other known genes involved in the determination of leaf width provided no evidence of their having any clear functional association with qFLW4/NAL1 .
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[ Sen. 2, subscore: 1.00 ]: QTL mapping in a recombinant inbred line population derived from the cross between the javanica cultivar D50 ( narrow-leaved ) and the indica cultivar HB277 ( wide-leaved ) identified five QTLs controlling flag leaf width .
[ Sen. 4, subscore: 1.00 ]: There was no difference in the level of NAL1 expression between cvs D50 and HB277 , but an analysis of the NAL1 transcripts showed that while most ( if not all ) of those produced in cv .
[ Sen. 5, subscore: 1.00 ]: D50 were full-length , two-thirds of those in HB277 were non-functional due to either loss or gain of sequence .
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| Title: Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis ( Bt ) Cry1B toxin .
| | Author: Zhang X Liu Y Zhang C Wang Y Xu C Liu X | | Journal: Ecotoxicol Environ Saf Citation: V : 81 P : 84-90 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22627013 Accession (PMID): 22627013 | | Abstract: Single chain variable fragment antibody ( scFv ) is capable of binding its target antigens and is one of the most popular recombinant antibodies format for many applications .
In this study , a large human synthetic phage displayed library ( Tomlinson J ) was employed to generate scFvs against Cry1B toxin by affinity panning .
After four rounds of panning , six monoclonal phage particles capable of binding with the Cry1B were isolated , sequenced and characterized by Enzyme-Linked Immunosorbent Assay ( ELISA ) .
Two of the identified novel anti-Cry1B scFvs , namely H9 and B12 , were expressed in Escherichia coli HB2151 and purified by Ni metal ion affinity chromatography .
Sodium dodecyl sulfate polyacrylamine gel electrophoresis ( SDS-PAGE ) indicated that the relative molecular mass of scFv was estimated at 30 kDa .
The purified scFv-H9 was used to develop an indirect competitive enzyme-linked immunosorbent assay ( ic-ELISA ) for Cry1B toxin .
The linear range of detection for standards in this ic-ELISA was approximately 0 . 19-1 . 1 mug mL ( - ) ( 1 ) and 50% inhibition of control ( IC ( 5 ) ( 0 ) ) was 0 . 84 mug mL ( - ) ( 1 ) for Cry1B .
The affinity of scfv-H9 was ( 1 . 95+/-0 . 12 ) x 10 ( 7 ) M ( - ) ( 1 ) and showed cross-reactivity with Cry1Ab toxin and Cry1Ac toxin ( 8 . 53% and 7 . 58% , respectively ) , higher cross-reactivity ( 12 . 8% ) with Cry1C toxin .
The average recoveries of Cry1B toxin from spiked leaf and rice samples were in the range 89 . 5-96 . 4% , and 88 . 5-95 . 6% , respectively , with a coefficient of variation ( CV ) less than 6 . 0% .
These results showed promising applications of scfv-H9 for detecting Cry1B toxin in agricultural and environmental samples .
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[ Sen. 4, subscore: 1.00 ]: Two of the identified novel anti-Cry1B scFvs , namely H9 and B12 , were expressed in Escherichia coli HB2151 and purified by Ni metal ion affinity chromatography .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
