| 21 matches found in 9 documents. Search time: 0.041 seconds. |
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| Title: Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants .
| | Author: Sato Y Sentoku N Miura Y Hirochika H Kitano H Matsuoka M | | Journal: EMBO J Citation: V : 18 ( 4 ) P : 992-1002 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10022841 Accession (PMID): 10022841 | | Abstract: The rice homeobox gene OSH15 ( Oryza sativa homeobox ) is a member of the knotted1-type homeobox gene family .
We report here on the identification and characterization of a loss-of-function mutation in OSH15 from a library of retrotransposon-tagged lines of rice .
Based on the phenotype and map position , we have identified three independent deletion alleles of the locus among conventional morphological mutants .
All of these recessive mutations , which are considered to be null alleles , exhibit defects in internode elongation .
Introduction of a 14 kbp genomic DNA fragment that includes all exons , introns and 5- and 3- flanking sequences of OSH15 complemented the defects in internode elongation , confirming that they were caused by the loss-of-function of OSH15 .
Internodes of the mutants had abnormal-shaped epidermal and hypodermal cells and showed an unusual arrangement of small vascular bundles .
These mutations demonstrate a role for OSH15 in the development of rice internodes .
This is the first evidence that the knotted1-type homeobox genes have roles other than shoot apical meristem formation and/or maintenance in plant development . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Introduction of a 14 kbp genomic DNA fragment that includes all exons , introns and 5- and 3- flanking sequences of OSH15 complemented the defects in internode elongation , confirming that they were caused by the loss-of-function of OSH15 .
[ Sen. 1, subscore: 1.00 ]: The rice homeobox gene OSH15 ( Oryza sativa homeobox ) is a member of the knotted1-type homeobox gene family .
[ Sen. 2, subscore: 1.00 ]: We report here on the identification and characterization of a loss-of-function mutation in OSH15 from a library of retrotransposon-tagged lines of rice .
[ Sen. 7, subscore: 1.00 ]: These mutations demonstrate a role for OSH15 in the development of rice internodes .
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| Title: KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice .
| | Author: Ito Y Eiguchi M Kurata N | | Journal: Genesis Citation: V : 30 ( 4 ) P : 231-8 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11536429 Accession (PMID): 11536429 | | Abstract: We produced transgenic rice calli , which constitutively express each of four KNOX family class 1 homeobox genes of rice , OSH1 , OSH16 , OSH15 , and OSH71 , and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli , which showed continuous growth around their shoot-regenerating stages .
Transgenic calli transferred onto regeneration medium began to display green spots , a sign of regeneration , but most of the transformants continued to propagate green spots at given stages .
In the normal shoot-regeneration process of calli , expression of endogenous OSH1 was restricted in presumptive shoot-regenerating regions of calli and not observed in other areas .
This restricted expression pattern should be required for further differentiation of the regenerating shoots .
Thus our present results support the proposed function that KNOX family class 1 homeobox genes play a role in the formation and maintenance of the undetermined meristematic state of cells . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We produced transgenic rice calli , which constitutively express each of four KNOX family class 1 homeobox genes of rice , OSH1 , OSH16 , OSH15 , and OSH71 , and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli , which showed continuous growth around their shoot-regenerating stages .
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Score: 3.00 |
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| Title: Functional analysis of the conserved domains of a rice KNOX homeodomain protein , OSH15 .
| | Author: Nagasaki H Sakamoto T Sato Y Matsuoka M | | Journal: Plant Cell Citation: V : 13 ( 9 ) P : 2085-98 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11549765 Accession (PMID): 11549765 | | Abstract: The rice KNOX protein OSH15 consists of four conserved domains : the MEINOX domain , which can be divided into two subdomains ( KNOX1 and KNOX2 ) ; the GSE domain ; the ELK domain ; and the homeodomain ( HD ) .
To investigate the function of each domain , we generated 10 truncated proteins with deletions in the conserved domains and four proteins with mutations in the conserved amino acids in the HD .
Transgenic analysis suggested that KNOX2 and HD are essential for inducing the abnormal phenotype and that the KNOX1 and ELK domains affect phenotype severity .
We also found that both KNOX2 and HD are necessary for homodimerization and that only HD is needed for binding of OSH15 to its target sequence .
Transactivation studies suggested that both the KNOX1 and ELK domains play a role in suppressing target gene expression .
On the basis of these findings , we propose that overproduced OSH15 probably acts as a dimer and may ectopically suppress the expression of target genes that induce abnormal morphology in transgenic plants . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice KNOX protein OSH15 consists of four conserved domains : the MEINOX domain , which can be divided into two subdomains ( KNOX1 and KNOX2 ) ; the GSE domain ; the ELK domain ; and the homeodomain ( HD ) .
[ Sen. 4, subscore: 1.00 ]: We also found that both KNOX2 and HD are necessary for homodimerization and that only HD is needed for binding of OSH15 to its target sequence .
[ Sen. 6, subscore: 1.00 ]: On the basis of these findings , we propose that overproduced OSH15 probably acts as a dimer and may ectopically suppress the expression of target genes that induce abnormal morphology in transgenic plants .
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Score: 3.00 |
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| Title: Members of TALE and WUS subfamilies of homeodomain proteins with potentially important functions in development form dimers within each subfamily in rice .
| | Author: Nagasaki H Matsuoka M Sato Y | | Journal: Genes Genet .
Syst Citation: V : 80 ( 4 ) P : 261-7 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16284419 Accession (PMID): 16284419 | | Abstract: Transacting factors often form homo and heterodimers and regulate various targets , the type of regulation depending on the dimeric combination .
The WUS and TALE subfamilies are two atypical homeodomains in plants .
A homeodomain mediates sequence-specific binding to its target DNA and usually consists of 60 amino acid residues , whereas atypical homeodomains have extra amino acid residues in the well-conserved region .
The genes OsWUS and OsPRS , which encode atypical homeodomain proteins from the WUS subfamily , and OsBEL and OSH15 , which encode those from the TALE subfamily , were isolated from rice and tested for their interactions by yeast two-hybrid analysis .
OsWUS and OsPRS formed homodimers and formed heterodimers with each other but did not form dimers with the TALE family homeodomain proteins OSH15 or OsBEL .
Likewise , OSH15 and OsBEL formed homodimers and heterodimers but did not form dimers with the WUS family homeodomain proteins OsWUS and OsPRS .
These findings suggest that the combinations of dimers are well correlated with the classification of these proteins on the basis of sequence similarity .
RT-PCR analysis revealed that expression of OsWUS and OsPRS was detected in the same organs , namely floral buds , roots , and suspension cells .
Therefore , it is possible that the proteins encoded by both of these genes function as homo and heterodimers in planta .
These results suggest that , during the evolution of these subfamilies , various combinations of dimers within proteins encoded by paralogous genes were formed and generated independent regulatory networks that enabled complex patterns of plant development . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The genes OsWUS and OsPRS , which encode atypical homeodomain proteins from the WUS subfamily , and OsBEL and OSH15 , which encode those from the TALE subfamily , were isolated from rice and tested for their interactions by yeast two-hybrid analysis .
[ Sen. 5, subscore: 1.00 ]: OsWUS and OsPRS formed homodimers and formed heterodimers with each other but did not form dimers with the TALE family homeodomain proteins OSH15 or OsBEL .
[ Sen. 6, subscore: 1.00 ]: Likewise , OSH15 and OsBEL formed homodimers and heterodimers but did not form dimers with the WUS family homeodomain proteins OsWUS and OsPRS .
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Score: 2.00 |
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| Title: Positive Autoregulation of a KNOX Gene Is Essential for Shoot Apical Meristem Maintenance in Rice .
| | Author: Tsuda K Ito Y Sato Y Kurata N | | Journal: Plant Cell Citation: V : P : Year: 2011 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22207572 Accession (PMID): 22207572 | | Abstract: Self-maintenance of the shoot apical meristem ( SAM ) , from which aerial organs are formed throughout the life cycle , is crucial in plant development .
Class I Knotted1-like homeobox ( KNOX ) genes restrict cell differentiation and play an indispensable role in maintaining the SAM .
However , the mechanism that positively regulates their expression is unknown .
Here , we show that expression of a rice ( Oryza sativa ) KNOX gene , Oryza sativa homeobox1 ( OSH1 ) , is positively regulated by direct autoregulation .
Interestingly , loss-of-function mutants of OSH1 lose the SAM just after germination but can be rescued to grow until reproductive development when they are regenerated from callus .
Double mutants of osh1 and d6 , a loss-of-function mutant of OSH15 , fail to establish the SAM both in embryogenesis and regeneration .
Expression analyses in these mutants reveal that KNOX gene expression is positively regulated by the phytohormone cytokinin and by KNOX genes themselves .
We demonstrate that OSH1 directly binds to five KNOX loci , including OSH1 and OSH15 , through evolutionarily conserved cis-elements and that the positive autoregulation of OSH1 is indispensable for its own expression and SAM maintenance .
Thus , the maintenance of the indeterminate state mediated by positive autoregulation of a KNOX gene is an indispensable mechanism of self-maintenance of the SAM .
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[ Sen. 6, subscore: 1.00 ]: Double mutants of osh1 and d6 , a loss-of-function mutant of OSH15 , fail to establish the SAM both in embryogenesis and regeneration .
[ Sen. 8, subscore: 1.00 ]: We demonstrate that OSH1 directly binds to five KNOX loci , including OSH1 and OSH15 , through evolutionarily conserved cis-elements and that the positive autoregulation of OSH1 is indispensable for its own expression and SAM maintenance .
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