| 29 matches found in 14 documents. Search time: 0.135 seconds. |
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| Title: Sequence analysis of Pns11 , a nonstructural protein of rice gall dwarf virus , and its expression and detection in infected rice plants and vector insects .
| | Author: Moriyasu Y Ishikawa K Kikuchi A Imanishi S Tomita S Akutsu K Omura T | | Citation: V : 20 ( 3 ) P : 237-41 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10949951 Accession (PMID): 10949951 | | Abstract: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined .
The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus .
A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA .
An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects .
However , the antiserum did not react with purified viral proteins .
These results suggest that S11 encodes a nonstructural protein of RGDV .
This protein was named Pns11 . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined . The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus . A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV . This protein was named Pns11 .
[ Sen. 3, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined . The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus . A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV . This protein was named Pns11 .
[ Sen. 6, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined . The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus . A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV . This protein was named Pns11 .
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Score: 1.00 |
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| Title: Two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance .
| | Author: Tan GX Weng QM Ren X Huang Z Zhu LL He GC .
| | Citation: V : 92 ( 3 ) P : 212-7 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14666132 Accession (PMID): 14666132 | | Abstract: The whitebacked planthopper ( WBPH ) , Sogatella furcifera , and brown planthopper ( BPH ) Nilaparvata lugens Stl are important sucking insects of rice ( Oryza sativa L ) crops throughout the world .
Rice B5 , which has derived its resistance genes from the wild rice O officinalis Wall ex Watt , is a line that is highly resistant to both WBPH and BPH .
Previously , two resistance genes against BPH , Qbp1 , and Qbp2 in B5 had been mapped onto chromosome 3 and chromosome 4 , respectively .
In this study , we employed a mapping population composed of 187 recombinant inbred lines ( RILs ) , produced from a cross between B5 and susceptible variety Minghui63 , to locate the WBPH and BPH resistance genes .
A RFLP survey of the bulked extremes from the RIL population identified two genomic regions , one on chromosome 3 and the other on chromosome 4 , likely containing the resistance genes to planthoppers .
QTL analysis of the RILs further confirmed that two WBPH resistance genes were mapped on the same loci as Qbp1 and Qbp2 , using a linkage map with 242 molecular markers distributed on 12 rice chromosomes .
Of the two WBPH resistance genes , one designated Wbph7 ( t ) was located within a 1 . 1-cM region between R1925 and G1318 on chromosome 3 , the other designated Wbph8 ( t ) was within a 0 . 3-cM region flanked by R288 and S11182 on chromosome 4 .
A two-way analysis of variance showed that two loci acted independently with each other in determining WBPH resistance .
The results have significant implications in studying the interactions between sucking insects and plants and in breeding programs of resistance to rice planthoppers . | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The whitebacked planthopper ( WBPH ) , Sogatella furcifera , and brown planthopper ( BPH ) Nilaparvata lugens Stl are important sucking insects of rice ( Oryza sativa L ) crops throughout the world . Rice B5 , which has derived its resistance genes from the wild rice O officinalis Wall ex Watt , is a line that is highly resistant to both WBPH and BPH . Previously , two resistance genes against BPH , Qbp1 , and Qbp2 in B5 had been mapped onto chromosome 3 and chromosome 4 , respectively . In this study , we employed a mapping population composed of 187 recombinant inbred lines ( RILs ) , produced from a cross between B5 and susceptible variety Minghui63 , to locate the WBPH and BPH resistance genes . A RFLP survey of the bulked extremes from the RIL population identified two genomic regions , one on chromosome 3 and the other on chromosome 4 , likely containing the resistance genes to planthoppers . QTL analysis of the RILs further confirmed that two WBPH resistance genes were mapped on the same loci as Qbp1 and Qbp2 , using a linkage map with 242 molecular markers distributed on 12 rice chromosomes . Of the two WBPH resistance genes , one designated Wbph7 ( t ) was located within a 1 . 1-cM region between R1925 and G1318 on chromosome 3 , the other designated Wbph8 ( t ) was within a 0 . 3-cM region flanked by R288 and S11182 on chromosome 4 . A two-way analysis of variance showed that two loci acted independently with each other in determining WBPH resistance . The results have significant implications in studying the interactions between sucking insects and plants and in breeding programs of resistance to rice planthoppers .
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Score: 1.00 |
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| Title: High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice ( Oryza sativa L ) .
| | Author: Yang H You A Yang Z Zhang F He R Zhu L He G | | Citation: V : 110 ( 1 ) P : 182-91 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15549231 Accession (PMID): 15549231 | | Abstract: Resistance to the brown planthopper ( BPH ) , Nilaparvata lugens Stal , a devastating sucking insect pest of rice , is an important breeding objective in rice improvement programs .
Bph15 , one of the 17 major BPH resistance genes so far identified in both cultivated and wild rice , has been identified in an introgression line , B5 , and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers C820 and S11182 .
In order to pave the way for positional cloning of this gene , we have developed a high-resolution genetic map of Bph15 by positioning 21 DNA markers in the target chromosomal region .
Mapping was based on a PCR-based screening of 9 , 472 F ( 2 ) individuals derived from a cross between RI93 , a selected recombinant inbred line of B5 bearing the resistance gene Bph15 , and a susceptible variety , Taichung Native 1 , in order to identify recombinant plants within the Bph15 region .
Recombinant F ( 2 ) individuals with the Bph15 genotype were determined by phenotype evaluation .
Analysis of recombination events in the Bph15 region delimited the gene locus to an interval between markers RG1 and RG2 that co-segregated with the M1 marker .
A genomic library of B5 was screened using these markers , and bacterial artificial chromosome clones spanning the Bph15 chromosome region were obtained .
An assay of the recombinants using the sub-clones of these clones in combination with sequence analysis delimited the Bph15 gene to a genomic segment of approximately 47 kb .
This result should serve as the basis for eventual isolation of the Bph15 resistance gene . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Resistance to the brown planthopper ( BPH ) , Nilaparvata lugens Stal , a devastating sucking insect pest of rice , is an important breeding objective in rice improvement programs . Bph15 , one of the 17 major BPH resistance genes so far identified in both cultivated and wild rice , has been identified in an introgression line , B5 , and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers C820 and S11182 . In order to pave the way for positional cloning of this gene , we have developed a high-resolution genetic map of Bph15 by positioning 21 DNA markers in the target chromosomal region . Mapping was based on a PCR-based screening of 9 , 472 F ( 2 ) individuals derived from a cross between RI93 , a selected recombinant inbred line of B5 bearing the resistance gene Bph15 , and a susceptible variety , Taichung Native 1 , in order to identify recombinant plants within the Bph15 region . Recombinant F ( 2 ) individuals with the Bph15 genotype were determined by phenotype evaluation . Analysis of recombination events in the Bph15 region delimited the gene locus to an interval between markers RG1 and RG2 that co-segregated with the M1 marker . A genomic library of B5 was screened using these markers , and bacterial artificial chromosome clones spanning the Bph15 chromosome region were obtained . An assay of the recombinants using the sub-clones of these clones in combination with sequence analysis delimited the Bph15 gene to a genomic segment of approximately 47 kb . This result should serve as the basis for eventual isolation of the Bph15 resistance gene .
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Score: 2.00 |
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| Title: Molecular analysis of six segments of Tobacco leaf enation virus , a novel phytoreovirus from tobacco | | Author: Picton A Potgieter C Rey ME .
| | Citation: V : ( ) P : Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17356907 Accession (PMID): 17356907 | | Abstract: Tobacco leaf enation virus ( TLEV ) is a putative member of the genus Phytoreovirus within the family Reoviridae .
Previous western blot analysis of structural viral proteins ( apparent molecular weights of 93 kDa ; 58 kDa ; 48 kDa ; 39 kDa and 36 kDa ) associated with TLEV , isolated from infected tobacco in South Africa , suggested that these proteins may correspond to structural Wound tumor virus ( WTV ) proteins .
To further establish the nature of this novel virus disease phenotype in tobacco , molecular characterization of six dsRNA components was undertaken .
Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method .
Results of this study revealed the conserved terminal sequence : 5GG ( U/C ) . . . UGAU 3 of segments S6-S12 , while adjacent to these conserved terminal sequences are imperfect inverted repeats ( 7-15 bp in length ) , both features being common to reoviruses .
The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined .
Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses , Rice gall dwarf virus ( RGDV ) , Rice dwarf virus ( RDV ) and WTV has shown nucleotide and predicted amino acid identities within the range of 30-60% .
TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available .
Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) .
The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus , distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Tobacco leaf enation virus ( TLEV ) is a putative member of the genus Phytoreovirus within the family Reoviridae . Previous western blot analysis of structural viral proteins ( apparent molecular weights of 93 kDa ; 58 kDa ; 48 kDa ; 39 kDa and 36 kDa ) associated with TLEV , isolated from infected tobacco in South Africa , suggested that these proteins may correspond to structural Wound tumor virus ( WTV ) proteins . To further establish the nature of this novel virus disease phenotype in tobacco , molecular characterization of six dsRNA components was undertaken . Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method . Results of this study revealed the conserved terminal sequence : 5GG ( U/C ) . . . UGAU 3 of segments S6-S12 , while adjacent to these conserved terminal sequences are imperfect inverted repeats ( 7-15 bp in length ) , both features being common to reoviruses . The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined . Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses , Rice gall dwarf virus ( RGDV ) , Rice dwarf virus ( RDV ) and WTV has shown nucleotide and predicted amino acid identities within the range of 30-60% . TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available . Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) . The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus , distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa .
[ Sen. 9, subscore: 1.00 ]: Tobacco leaf enation virus ( TLEV ) is a putative member of the genus Phytoreovirus within the family Reoviridae . Previous western blot analysis of structural viral proteins ( apparent molecular weights of 93 kDa ; 58 kDa ; 48 kDa ; 39 kDa and 36 kDa ) associated with TLEV , isolated from infected tobacco in South Africa , suggested that these proteins may correspond to structural Wound tumor virus ( WTV ) proteins . To further establish the nature of this novel virus disease phenotype in tobacco , molecular characterization of six dsRNA components was undertaken . Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method . Results of this study revealed the conserved terminal sequence : 5GG ( U/C ) . . . UGAU 3 of segments S6-S12 , while adjacent to these conserved terminal sequences are imperfect inverted repeats ( 7-15 bp in length ) , both features being common to reoviruses . The complete nucleotide sequences of segments S5 ( 2 , 610 bp ) , S7 ( 1 , 740 bp ) , S8 ( 1 , 439 bp ) , S10 ( 1 , 252 bp ) , S11 ( 1 , 187 bp ) and S12 ( 836 bp ) were determined . Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses , Rice gall dwarf virus ( RGDV ) , Rice dwarf virus ( RDV ) and WTV has shown nucleotide and predicted amino acid identities within the range of 30-60% . TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available . Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 ( 90 . 6 kDa ) ; S7 ( 58 . 1 kDa ) ; S8 ( 47 . 7 kDa ) ; S10 ( 39 . 8 kDa ) ; S11 ( 35 kDa ) and S12 ( 19 . 5 kDa ) . The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus , distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa .
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Score: 1.00 |
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| Title: Molecular characterization of the largest and smallest genome segments , S1 and S12 , of Rice gall dwarf virus .
| | Author: Zhang HM Yang J Xin X Chen JP Adams MJ | | Citation: V : 35 P : 815-23 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17674177 Accession (PMID): 17674177 | | Abstract: The nucleotide sequences of segments S1 and S12 of a Chinese isolate of Rice gall dwarf virus ( RGDV ) were determined .
This provides the first complete sequences of these segments .
The complete sequence of S1 , the largest genome segment of RGDV , was 4 , 505 nucleotides in length and was predicted to encode a large protein of 1 , 458 amino acids with a calculated molecular mass of nearly 166 . 2 kDa .
The protein was related to that encoded by S1 of Rice dwarf virus ( RDV ; 50% identity and 67% similarity ) and ( to a lesser extent ) to some large proteins of other reoviruses .
It appears to be an RNA-dependent RNA polymerase ( RdRp ) and is probably present in particles as a minor core protein .
S12 , the smallest genome segment of RGDV , was 853 nucleotides in length , encoding a single major protein of 206 amino acids with a calculated molecular mass of nearly 23 . 6 kDa .
This protein , though a little larger than those of RDV S11 and Wound tumor virus ( WTV ) S12 in size , showed some similarity to them , especially in the conserved N-terminal region and may have RNA-binding properties .
Despite having a common host plant , RDV and RGDV were not more closely related to one another than either of them was to WTV .
Phylogenetic analysis of the RdRp showed that members of the genus Phytoreovirus were more closely related to those of the genus Rotavirus than to any other genus within the family Reoviridae .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The nucleotide sequences of segments S1 and S12 of a Chinese isolate of Rice gall dwarf virus ( RGDV ) were determined . This provides the first complete sequences of these segments . The complete sequence of S1 , the largest genome segment of RGDV , was 4 , 505 nucleotides in length and was predicted to encode a large protein of 1 , 458 amino acids with a calculated molecular mass of nearly 166 . 2 kDa . The protein was related to that encoded by S1 of Rice dwarf virus ( RDV ; 50% identity and 67% similarity ) and ( to a lesser extent ) to some large proteins of other reoviruses . It appears to be an RNA-dependent RNA polymerase ( RdRp ) and is probably present in particles as a minor core protein . S12 , the smallest genome segment of RGDV , was 853 nucleotides in length , encoding a single major protein of 206 amino acids with a calculated molecular mass of nearly 23 . 6 kDa . This protein , though a little larger than those of RDV S11 and Wound tumor virus ( WTV ) S12 in size , showed some similarity to them , especially in the conserved N-terminal region and may have RNA-binding properties . Despite having a common host plant , RDV and RGDV were not more closely related to one another than either of them was to WTV . Phylogenetic analysis of the RdRp showed that members of the genus Phytoreovirus were more closely related to those of the genus Rotavirus than to any other genus within the family Reoviridae .
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