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Score: 1.00 | Title: Respiratory symptoms and pulmonary function among Chinese rice-granary workers .
| Author: Ye TT Huang JX Shen YE Lu PL Christiani DC .
| Journal: Citation: V : 4 ( 3 ) P : 155-9 Year: Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10026476 Accession (PMID): 10026476 | Abstract: The authors conducted a cross-sectional study of 474 rice-granary workers and 235 non-granary worker controls in a rural area near Shanghai , the Peoples Republic of China .
Responses to a respiratory-symptom questionnaire and pre and post-shift spirometry were obtained for all subjects .
Area sampling was performed for total and vertically elutriated ( = 15 micrometer ) dust levels .
Total dust levels were high , ranging from 6 . 6 mg/m ( 3 ) to 59 . 8 mg/m ( 3 ) , with vertical elutriated dust concentrations ranging from 2 . 0 to 10 .
4 mg/m ( 3 ) .
The granary workers reported significantly more respiratory symptoms , including chronic cough , sputum production , chronic bronchitis , grain fever ( ODTS ) , and nasal and skin irritation .
Grain dust and tobacco smoking were more than additive for the prevalence of chronic cough and chronic bronchitis .
After adjusting for confounders , the granary workers had lower mean FEV&inf1 ; /FVC values both pre and post-shift , indicating an association between chronic grain-dust exposure and chronic airway obstruction .
The results suggest that exposure to rice dust can induce pulmonary responses similar to those observed with exposures to other types of grains . | Matching Sentences: [ Sen. 9, subscore: 1.00 ]: The results suggest that exposure to rice dust can induce pulmonary responses similar to those observed with exposures to other types of grains .
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Score: 1.00 | Title: Evolutionary dynamics of Ty1-copia group retrotransposons in grass shown by reverse transcriptase domain analysis .
| Author: Matsuoka Y Tsunewaki K | Journal: Mol . Biol . Evol Citation: V : 16 ( 2 ) P : 208-17 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10028288 Accession (PMID): 10028288 | Abstract: The evolutionary dynamics of Ty1-copia group retrotransposons in grass were examined by reverse transcriptase ( RT ) domain analysis .
Twenty-three rice RT sequences were newly determined for this report .
Phylogenetic analysis of 177 RT sequences , mostly derived from wheat , rice , and , maize , showed four distinct families , which were designated G1 , G2 , G3 , and G4 .
Three of these families have elements obtained from distantly related species , indicative of origins prior to the radiation of grass species .
Results of Southern hybridization and detailed comparisons between the wheat and rice sequences indicated that each of the families had undergone a distinct pattern of evolution .
Multiple families appear to have evolved in parallel in a host species .
Analyses of synonymous and nonsynonymous substitutions suggested that there is a low percentage of elements carrying functional RT domains in the G4 family , indicating that the production of new G4 elements has been controlled by a small number of elements carrying functional RT domains . | Matching Sentences: [ Sen. 7, subscore: 1.00 ]: Analyses of synonymous and nonsynonymous substitutions suggested that there is a low percentage of elements carrying functional RT domains in the G4 family , indicating that the production of new G4 elements has been controlled by a small number of elements carrying functional RT domains .
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Score: 1.00 | Title: Gene cloning and expression of cytosolic glutathione reductase in rice ( Oryza sativa L ) .
| Author: Kaminaka H Morita S Nakajima M Masumura T Tanaka K | Journal: Plant Cell Physiol .
Citation: V : 39 ( 12 ) P : 1269-80 Year: 1998 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10050312 Accession (PMID): 10050312 | Abstract: We have isolated a cDNA ( RGRC2 ) encoding glutathione reductase ( GR ) from rice ( Oryza sativa L ) .
The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform .
The recombinant RGRC2 protein had enzymatic properties comparable to those of GR from rice embryo .
Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol . mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf it issues .
Southern blot analysis showed that the RGRC2 gene exists as a single copy gene .
Here , we have also isolated a genomic clone completely corresponding to RGRC2 .
The RGRC2 gene is split into 16 exons spread about 7 . 4 kb of chromosomal DNA , with coding sequence beginning in the 2nd exon and ending in the 16th exon .
From the presence of two ABA-responsive elements in the 5-flanking region of RGRC2 , we examined the expression in rice seedlings treated with ABA and the ABA-related environmental stresses , chilling , drought and salinity .
The expression of RGRC2 was strongly induced by all these treatments .
We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses . | Matching Sentences: [ Sen. 10, subscore: 1.00 ]: We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses .
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Score: 1.00 | Title: A "defeated" rice resistance gene acts as a QTL against a virulent strain of Xanthomonas oryzae pv . oryzae .
| Author: Li ZK Luo LJ Mei HW Paterson AH Zhao XH Zhong DB Wang YP Yu XQ Zhu L Tabien R Stansel JW Ying CS .
| Journal: Mol . Gen . Genet . Citation: V : 261 ( 1 ) P : 58-63 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10071210 Accession (PMID): 10071210 | Abstract: The genetic components responsible for qualitative and quantitative resistance of rice plants to three strains ( CR4 , CXO8 , and CR6 ) of Xanthomonas oryzae pv . oryzae ( Xoo ) were investigated using a set of 315 recombinant inbred lines ( RILs ) from the cross Lemont ( japonica ) x Teqing ( indica ) and a complete linkage map with 182 well distributed RFLP markers .
We mapped a major gene ( Xa4 ) and ten quantitative trait loci ( QTLs ) which were largely responsible for segregation of the resistance phenotype in the RILs .
The Teqing allele at the Xa4 locus , Xa4T , acted as a dominant resistance gene against CR4 and CXO8 .
The breakdown of Xa4T-associated resistance mediated by the mutant allele at the avrXa4 locus in the virulent strain CR6 results from significant changes in both gene action ( lose of dominance ) and the magnitude of gene effect ( approximately 50% reduction ) .
Nevertheless , Xa4T still acted as a recessive QTL with a significant residual effect against CR6 .
The mutant alleles at the avrXa4 locus in CXO8 and CR6 that lead to a reduction in effect , or "breakdown" , of Xa4T were apparently accompanied by corresponding penalties for their fitness .
The quantitative component of resistance to Xoo in the RILs was largely due to a number of resistance QTLs .
Most resistance QTLs mapped to genomic locations where major resistance genes and/or QTLs for resistance to Xoo , blast and sheath blight were identified in the same cross .
Most QTLs showed consistent levels of resistance against all three Xoo strains .
Our results suggest that a high level of durable resistance to Xoo may be achieved by the cumulative effects of multiple QTLs , including the residual effects of "defeated" major resistance genes . | Matching Sentences: [ Sen. 10, subscore: 1.00 ]: Our results suggest that a high level of durable resistance to Xoo may be achieved by the cumulative effects of multiple QTLs , including the residual effects of "defeated" major resistance genes .
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Score: 2.00 | Title: Digestibility index and factors affecting rate of starch digestion in vitro in conventional food preparation .
| Author: Urooj A Puttraj S | Journal: Citation: V : 43 ( 1 ) P : 42-7 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10079465 Accession (PMID): 10079465 | Abstract: The rate of starch hydrolysis in ten cereal-based food preparations was studied using an in vitro dialysis system .
The foods were incubated with human saliva and porcine pancreatin .
The sugars released after 3 h digestion were expressed as digestibility index ( DI ) , the percentage starch digested was determined and correlated with the degree of gelatinization ( DG ) .
Granule morphology was also investigated and related with starch availability for hydrolysis .
Significant differences were observed in the in vitro starch digestibility of the 10 foods ( P < 0 . 05 ) .
The DI ranged from 53 for chapathi to 78 for rice flakes .
DI was inversely related to the protein ( r = -0 . 79 , P < 0 . 01 ) , fat ( r = -0 . 63 , P < 0 . 05 ) and energy ( r = -0 . 61 , P < 0 . 01 ) .
Percent starch digested was inversely related to the insoluble ( r = -0 . 49 , P < 0 . 05 ) and total dietary fiber ( r = -0 . 63 , P < 0 . 01 ) content of the foods .
The SEM results provided a better understanding of granular morphology on cooking and the effect of protein on limiting DG .
The results suggest that carbohydrate foods of potential use in the therapeutic diets may be identified by their in vitro digestion characteristics .
| Matching Sentences: [ Sen. 3, subscore: 1.00 ]: The sugars released after 3 h digestion were expressed as digestibility index ( DI ) , the percentage starch digested was determined and correlated with the degree of gelatinization ( DG ) . [ Sen. 10, subscore: 1.00 ]: The results suggest that carbohydrate foods of potential use in the therapeutic diets may be identified by their in vitro digestion characteristics .
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Score: 2.00 | Title: Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis .
| Author: Postma-Haarsma AD Verwoert II Stronk OP Koster J Lamers GE Hoge JH Meijer AH .
| Journal: Plant Mol . Biol . Citation: V : 39 ( 2 ) P : 257-71 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10080693 Accession (PMID): 10080693 | Abstract: For identification of genes involved in embryogenesis in the model cereal rice , we have constructed a collection of cDNA libraries of well-defined stages of embryo development before , during and after organ differentiation .
Here , we focus on the possible role of KNOX ( maize Knotted1-like ) class homeobox genes in regulation of rice embryogenesis .
Three types of KNOX clones were identified in libraries of early zygotic embryos .
Two of these , Oskn2 and Oskn3 , encode newly described KNOX genes , whereas the third ( Oskn1 ) corresponds to the previously described OSH1 gene .
In situ hybridizations showed that during the early stages of embryo development , all three KNOX genes are expressed in the region where the shoot apical meristem ( SAM ) is organizing , suggesting that these genes are involved in regulating SAM formation .
Whereas OSH1 was previously proposed to function also in SAM maintenance , Oskn3 may be involved in patterning organ positions , as its expression was found to mark the boundaries of different embryonic organs following SAM formation .
The expression pattern of Oskn2 suggested an additional role in scutellum and epiblast development .
Transgenic expression of Oskn2 and Oskn3 in tobacco further supported their involvement in cell fate determination , like previously reported for Knotted1 and OSH1 ectopic expression .
Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development , Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology .
The observation that the KNOX genes produce similar though distinct phenotypic reponses in tobacco , indicates that their gene products act on overlapping but different sets of target genes , or that cell-type specific factors determine their precise action . | Matching Sentences: [ Sen. 5, subscore: 1.00 ]: In situ hybridizations showed that during the early stages of embryo development , all three KNOX genes are expressed in the region where the shoot apical meristem ( SAM ) is organizing , suggesting that these genes are involved in regulating SAM formation . [ Sen. 7, subscore: 1.00 ]: The expression pattern of Oskn2 suggested an additional role in scutellum and epiblast development .
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Score: 1.00 | Title: Molecular characterization of catalytic-subunit cDNA sequences encoding protein phosphatases 1 and 2A and study of their roles in the gibberellin-dependent Osamy-c expression in rice .
| Author: Chang M Wang B Chen X Wu R | Journal: Plant Mol . Biol . Citation: V : 39 ( 1 ) P : 105-15 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10080713 Accession (PMID): 10080713 | Abstract: To understand the molecular mechanism of gibberellin-dependent gene regulation , the effect of three phosphatase inhibitors on the germination of rice seeds and the expression of a target gene , the alpha-amylase gene , Osamy-c , were measured .
We found that okadaic acid , microcystin-LR , and calyculin A , which are known to specifically inhibit Ser/Thr phosphatases 1 and 2A , strongly inhibit the expression of the Osamy-c and may be involved in the germination of rice seeds .
The protein phosphatase enzyme activity assays showed that there is no obvious effect of GA3 on total PP1/PP2A activities .
To further understand the possible role of protein phosphatases 1 and 2A in the GA-dependent expression of Osamy-c , we isolated cDNA clones encoding protein phosphatase 1 and protein phosphatase 2A from a rice aleurone cDNA library .
These were designated OsPP1c and OsPP2Ac , respectively .
Comparison of the deduced amino acid sequences of OsPP1c and OsPP2Ac with the catalytic subunits of PP1 or PP2A of rabbit skeletal muscle , Arabidopsis thaliana , maize and Brassica napus showed that the catalytic subunit sequences of PP1 or PP2A among these organisms are highly conserved ( 73% to 90% similarity ) .
Genomic Southern blot analysis indicated that there are only one or two copies of OsPP1c genes and more than two copies of OsPP2Ac genes in the rice genome .
Northern blot analysis showed that OsPP1c and OsPP2Ac genes are expressed in several organs of rice , including seed , shoot and root .
We also showed by using 3 gene-specific probes of OsPP1c and OsPP2Ac cDNA , that the expression of neither gene is regulated by GA .
Taken together , our results suggest that protein phosphatases PP1 or PP2A are involved in the GA-dependent expression of the rice Osamy-c gene , though the PP1 or/and PP2A enzymatic activities as well as mRNA levels do not increase upon GA3 treatment . | Matching Sentences: [ Sen. 10, subscore: 1.00 ]: Taken together , our results suggest that protein phosphatases PP1 or PP2A are involved in the GA-dependent expression of the rice Osamy-c gene , though the PP1 or/and PP2A enzymatic activities as well as mRNA levels do not increase upon GA3 treatment .
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Score: 1.00 | Title: Molecular characterization of a gene for alanine aminotransferase from rice ( Oryza sativa ) .
| Author: Kikuchi H Hirose S Toki S Akama K Takaiwa F | Journal: Plant Mol . Biol . Citation: V : 39 ( 1 ) P : 149-59 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10080717 Accession (PMID): 10080717 | Abstract: A cDNA clone encoding alanine aminotransferase ( AlaAT ) has isolated from randomly sequenced clones derived from a cDNA library of maturing rice seeds by comparison to previously identified genes .
The deduced amino acid sequence was 88% and 91% homologous to those of the enzymes from barley and broomcorn millet ( Panicum miliaceum ) , respectively .
Using this cDNA as a probe , we isolated and sequenced the corresponding genomic clone .
Comparison of the sequences of the cDNA and the genomic gene revealed that the coding region of the gene was interrupted by 14 introns 66 to 1547 bp long .
Northern and western blotting analyses showed that the gene was expressed at high levels in developing seeds .
When the 5-flanking region between -930 and +85 from the site of initiation of transcription was fused to a reporter gene for beta-glucuronidase ( GUS ) and then introduced into the rice genome , histochemical staining revealed strong GUS activity in the inner endosperm it issue of developing seeds and weak activity in root tips .
Similar it issue-specific expression was also detected by in situ hybridization .
These results suggest that AlaAT is involved in nitrogen metabolism during the maturation of rice seed . | Matching Sentences: [ Sen. 8, subscore: 1.00 ]: These results suggest that AlaAT is involved in nitrogen metabolism during the maturation of rice seed .
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Score: 1.00 | Title: Modified starch enhances absorption and accelerates recovery in experimental diarrhea in rats .
| Author: Wingertzahn MA Teichberg S Wapnir RA .
| Journal: Pediatr . Res . Citation: V : 45 ( 3 ) P : 397-402 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10088661 Accession (PMID): 10088661 | Abstract: Rice gruels have been used as home remedies to treat dehydration associated with diarrheal illness in developing countries .
These preparations have produced conflicting results , most likely due to the heterogeneity of starch used .
We investigated whether the modified tapioca starch , Textra ( TX ) , at 5 . 0 or 10 . 0 g/L added to a 90 mmol/L Na+-111 mmol glucose oral rehydration solution ( ORS ) enhanced water and electrolyte absorption in two models of diarrhea .
To induce a secretory state ( model A ) , the jejunum of juvenile rats was perfused with 10 mmol/L theophylline ( THEO ) under anesthesia and then perfused with the solutions indicated above .
To produce chronic osmotic-secretory diarrhea ( model B ) , rats had a magnesium citrate-phenolphthalein solution as the sole fluid source for 1 wk , and then were perfused as the THEO-treated rats .
Water , electrolyte , and glucose absorption were measured during both perfusions .
As an extension of the perfusion studies , we compared how fast rats recovered from chronic osmotic diarrhea by offering them either water , ORS , or ORS containing 5 . 0 g/L TX along with solid food .
Recovery rate markers were measured after 24 h and included weight gain , food and fluid intake , and stool output .
In model A , addition of 5 . 0 g/L TX to ORS reversed Na+ secretion and improved net water as well as K+ and glucose absorption , compared with THEO-treated rats perfused with ORS without TX .
In model B , addition of TX to ORS increased water , Na+ , K+ , and glucose absorption , compared with rats perfused without TX .
Increasing TX from 5 . 0 to 10 . 0 g/L had no additional benefit .
In recovery experiments , animals with free access to ORS with TX had significantly greater weight gain and decreased stool output compared with animals recovering with water or ORS without TX .
Our experiments suggest that TX may be a useful additive to standard ORS to promote fluid and electrolyte absorption and may provide additional energy without increasing ORS osmotic load .
| Matching Sentences: [ Sen. 13, subscore: 1.00 ]: Our experiments suggest that TX may be a useful additive to standard ORS to promote fluid and electrolyte absorption and may provide additional energy without increasing ORS osmotic load .
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Score: 1.00 | Title: New lead compounds for brassinosteroid biosynthesis inhibitors .
| Author: Min YK Asami T Fujioka S Murofushi N Yamaguchi I Yoshida S | Journal: Bioorg . Med . Chem . Lett . Citation: V : 9 ( 3 ) P : 425-30 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10091696 Accession (PMID): 10091696 | Abstract: The first brassinosteroid biosynthesis inhibitor is reported .
Among newly synthesized triazole derivatives , 4- ( 4-chlorophenyl ) -2-phenyl-3- ( 1 , 2 , 4-triazoyl ) butan-2-ol ( 6 ) was found to inhibit the growth of cress seedlings , and this inhibition was recovered by the treatment of brassinolide , suggesting that compound 6 primarily inhibits brassinosteroid biosynthesis . | Matching Sentences: [ Sen. 2, subscore: 1.00 ]: Among newly synthesized triazole derivatives , 4- ( 4-chlorophenyl ) -2-phenyl-3- ( 1 , 2 , 4-triazoyl ) butan-2-ol ( 6 ) was found to inhibit the growth of cress seedlings , and this inhibition was recovered by the treatment of brassinolide , suggesting that compound 6 primarily inhibits brassinosteroid biosynthesis .
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