| 45 matches found in 19 documents. Search time: 0.014 seconds. |
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| Title: Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice ( Oryza sativa ) .
| | Author: Sirithunya P Tragoonrung S Vanavichit A Pa-In N Vongsaprom C Toojinda T | | Journal: DNA Res .
Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12168952 Accession (PMID): 12168952 | | Abstract: Blast is an economically important disease of rice .
To map genes controlling blast resistance , recombinant inbred lines ( RIL ) were developed from Khao Dawk Mali 105 , an aromatic , blast-susceptible cultivar and the blast resistance donor , CT 9993-5-10-M ( CT ) .
A linkage map encompassing 2112 cM was constructed from 141 RILs using 90 restriction fragment length polymorphisms ( RFLPs ) and 31 simple sequence repeats ( SSR ) .
Virulent isolates of blast fungus were identified by screening differential host sets against 87 single-spore isolates collected from the north and northeast of Thailand .
Fifteen virulent blast isolates were selected for leaf blast screening .
Neck blast was evaluated both under natural conditions and controlled inoculations .
Quantitative trait loci ( QTLs ) for broad resistance spectrum ( BRS ) to leaf blast were located on chromosomes 7 and 9 .
In particular , the QTL ( ch9 ) was mapped near the Pi5 ( t ) locus .
The QTL ( ch7 ) was located close to a previously mapped partial resistance QTL .
Both loci showed significant allelic interaction .
Genotypes having CT alleles at both QTL ( ch7 ) and QTL ( ch9 ) were the most resistant .
Two neck-blast QTLs were mapped on chromosomes 5 and 6 .
The inconsistent map locations between the leaf and neck blast QTLs indicate the complexity of fixing both leaf and neck blast resistance .
The coincidence of BRS and field resistance QTLs on chromosome 7 supports the idea that BRS may reflect the broad resistance spectrum to leaf blast in rice .
These findings laid the foundation for the development of a marker-assisted scheme for improving Khoa Dawk Mali 105 and the majority of aromatic Thai rice varieties that are susceptible to blast | |
Score: 7.00 |
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| Title: Genetic and physical mapping of Pi5 ( t ) , a locus associated with broad-spectrum resistance to rice blast | | Author: Jeon JS Chen D Yi GH Wang GL Ronald PC .
| | Journal: Mol . Genet . Genomics Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12756540 Accession (PMID): 12756540 | | Abstract: To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea ) , we have initiated a project to clone Pi5 ( t ) , a locus associated with broad-spectrum resistance to diverse blast isolates .
AFLP-derived markers linked to Pi5 ( t ) -mediated resistance were isolated using bulked segregant analysis of F ( 2 ) populations generated by crossing three recombinant inbred lines ( RILs ) , RIL125 , RIL249 , and RIL260 with the susceptible line CO39 .
The most tightly linked AFLP marker , S04G03 , was positioned on chromosome 9 of the fingerprint-based physical map of Nipponbare , a well-characterized rice genotype .
Flanking BAC-based Nipponbare markers were generated for saturation mapping using four populations , the three initial RILs and an additional one derived from a cross between M202 and RIL260 .
A BIBAC ( binary BAC ) library was constructed from RIL260 .
Using these resources Pi5 ( t ) was mapped to a 170-kb interval , and a contiguous set of BIBAC clones spanning this region was constructed .
It had previously been suggested that Pi3 ( t ) and Pi5 ( t ) might be allelic , due to their identical resistance spectrum and tight linkage .
We therefore compared genomic regions for lines containing Pi3 ( t ) using the Pi5 ( t ) -linked markers .
DNA gel-blot analyses indicated that the region around Pi3 ( t ) is identical to that of Pi5 ( t ) , suggesting that Pi3 ( t ) and Pi5 ( t ) are the same resistance gene . | |
Score: 6.00 |
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| Title: Use of Pi5 ( t ) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea .
| | Author: Yi G Lee SK Hong YK Cho YC Nam MH Kim SC Han SS Wang GL Hahn TR Ronald PC Jeon JS .
| | Journal: Theor . Appl . Genet . Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15141293 Accession (PMID): 15141293 | | Abstract: Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection ( MAS ) .
The rice Pi5 ( t ) locus confers broad-spectrum resistance to Magnaporthe grisea , the causal agent of rice blast disease .
It has been hypothesized that the Pi5 ( t ) locus carries the same gene as that encoded by the Pi3 ( t ) and Pii ( t ) loci .
We developed three PCR-based dominant markers ( JJ80-T3 , JJ81-T3 , and JJ113-T3 ) from three previously identified BIBAC clones-JJ80 , JJ81 , and JJ113-that are linked to the Pi5 ( t ) locus .
PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 ( t ) , Pi3 ( t ) , and Pii ( t ) .
PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3 , JJ81-T3 , and JJ113-T3 indicated that Tetep is the likely donor of Pi5 ( t ) .
Of the 184 rice varieties tested , 34 carried the JJ80-T3- , JJ81-T3- , and JJ113-T3-specific bands .
Disease evaluation of those 34 varieties revealed that all conferred resistance to PO6-6 .
The genomic structure of three of these resistant varieties ( ie , IR72 , Taebaeg , Jahyangdo ) is most similar to that of Pi5 ( t ) .
Our results demonstrate the usefulness of the JJ80-T3 , JJ81-T3 , and JJ113-T3 markers for MAS for M grisea resistance . | |
Score: 1.00 |
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| Title: The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding .
| | Author: Lou Y Ma H Lin WH Chu ZQ Mueller-Roeber B Xu ZH Xue HW .
| | Journal: Plant Mol . Biol . Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16649109 Accession (PMID): 16649109 | | Abstract: In Arabidopsis thaliana and Oryza sativa , two types of PI 4-kinase ( PI4Ks ) have been isolated and functionally characterized .
The alpha-type PI4Ks ( approximately 220 kDa ) contain a PH domain , which is lacking in beta-type PI4Ks ( approximately 120 kDa ) .
Beta-type PI4Ks , exemplified by Arabidopsis AtPI4Kbeta and rice OsPI4K2 , contain a highly charged repetitive segment designated PPC ( Plant PI4K Charged ) region , which is an unique domain only found in plant beta-type PI4Ks at present .
The PPC region has a length of approximately 300 amino acids and harboring 11 ( AtPI4Kbeta ) and 14 ( OsPI4K2 ) repeats , respectively , of a 20-aa motif .
Studies employing a modified yeast-based "Sequence of Membrane-Targeting Detection" system demonstrate that the PPC ( OsPI4K2 ) region , as well as the former 8 and latter 6 repetitive motifs within the PPC region , are able to target fusion proteins to the plasma membrane .
Further detection on the transiently expressed GFP fusion proteins in onion epidermal cells showed that the PPC ( OsPI4K2 ) region alone , as well as the region containing repetitive motifs 1-8 , was able to direct GFP to the plasma membrane , while the regions containing less repetitive motifs , ie 6 , 4 , 2 or single motif ( s ) led to predominantly intracellular localization .
Agrobacterium-mediated transient expression of PPC-GFP fusion protein further confirms the membrane-targeting capacities of PPC region .
In addition , the predominant plasma membrane localization of AtPI4Kbeta was mediated by the PPC region .
Recombinant PPC peptide , expressed in E coli , strongly binds phosphatidic acid , PI and PI4P , but not phosphatidylcholine , PI5P , or PI ( 4 , 5 ) P2 in vitro , providing insights into potential mechanisms for regulating sub-cellular localization and lipid binding for the plant beta-type PI4Ks . | |
Score: 11.00 |
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| Title: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
| | Author: Lee SK Song MY Seo YS Kim HK Ko S Cao PJ Suh JP Yi G Roh JH Lee S An G Hahn TR Wang GL Ronald P Jeon JS | | Journal: Genetics Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19153255 Accession (PMID): 19153255 | | Abstract: Rice blast , caused by the fungus Magnaporthe oryzae , is one of the most devastating diseases of rice .
To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy .
Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif .
In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae .
In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae .
Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed .
These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
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