| 110 matches found in 59 documents. Search time: 0.219 seconds. |
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| Title: Molecular variability of geographically distinct isolates of Rice yellow mottle virus in Africa .
| | Author: Pinel A NGuessan P Bousalem M Fargette D | | Journal: Arch . Virol . Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11003473 Accession (PMID): 11003473 | | Abstract: The coat protein gene ( ORF4 ) and the 3 untranslated region of a sample of 40 isolates of Rice yellow mottle virus ( RYMV ) , 32 from West Africa and 8 from East Africa , have been sequenced .
Five major strains were differentiated , three from West Africa ( S1 , S2 , S3 ) and two from East Africa ( S4 , S5 ) , with a spatial overlap of the strains within each of these two regions .
Nucleotide and amino-acid divergence between strains was up to 11% .
Although more isolates from West African were sequenced , variability was twofold lower than among East African isolates .
Variability in ORF4 and in ORF2 coincided .
Within strain and within isolate variations in nucleotide sequences were low .
Bipartite nuclear targeting motif , Ca2+ binding sites and at least two stretches of amino-acids were conserved among the 40 RYMV isolates and the other sobemoviruses .
Variants associating sequence motifs characteristic of different strains have been found , possibly resulting from recombination events .
Differences in pathogenicity among isolates were associated with changes of amino-acids in the bipartite nuclear targeting motif of the R domain of the capsid protein , and around conserved positions 151-154 of the S domain .
We hypothesise that the observed pattern of variation of RYMV reflects the effect of spatial isolation between East and West Africa coupled with adaptive changes associated to the original virus reservoirs of the different strains . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Five major strains were differentiated , three from West Africa ( S1 , S2 , S3 ) and two from East Africa ( S4 , S5 ) , with a spatial overlap of the strains within each of these two regions .
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| Title: Molecular characterization of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4 .
| | Author: Ikeda K Nagaoka S Winkler S Kotani K Yagi H Nakanishi K Miyajima S Kobayashi J Mori H | | Journal: J Virol .
Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11134312 Accession (PMID): 11134312 | | Abstract: The complete nucleotide sequence of the genome segment 4 ( S4 ) of Bombyx mori cytoplasmic polyhedrosis virus ( BmCPV ) was determined .
The 3 , 259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kDa .
Western blot analysis showed that S4 encodes BmCPV protein VP3 , which is one of the outer components of the BmCPV virion .
Sequence analysis of the deduced amino acid sequence of BmCPV VP3 revealed possible sequence homology with proteins from rice ragged stunt virus ( RRSV ) S2 , Nilaparvata lugens reovirus S4 , and Fiji disease fijivirus S4 .
This may suggest that plant reoviruses originated from insect viruses and that RRSV emerged more recently than other plant reoviruses .
A chimeric protein consisting of BmCPV VP3 and green fluorescent protein ( GFP ) was constructed and expressed with BmCPV polyhedrin using a baculovirus expression vector .
The VP3-GFP chimera was incorporated into BmCPV polyhedra and released under alkaline conditions .
The results indicate that specific interactions occur between BmCPV polyhedrin and VP3 which might facilitate BmCPV virion occlusion into the polyhedra .
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[ Sen. 4, subscore: 1.00 ]: Sequence analysis of the deduced amino acid sequence of BmCPV VP3 revealed possible sequence homology with proteins from rice ragged stunt virus ( RRSV ) S2 , Nilaparvata lugens reovirus S4 , and Fiji disease fijivirus S4 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice .
| | Author: Adhikari TB Joseph CM Yang G Phillips DA Nelson LM .
| | Journal: Can .
J Microbiol .
Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11718545 Accession (PMID): 11718545 | | Abstract: Of 102 rhizoplane and endophytic bacteria isolated from rice roots and stems in California , 37% significantly ( P < or = 0 . 05 ) inhibited the growth in vitro of two pathogens , Achlya klebsiana and Pythium spinosum , causing seedling disease of rice .
Four endophytic strains were highly effective against seedling disease in growth pouch assays , and these were identified as Pseudomonas fluorescens ( S3 ) , Pseudomonas tolaasii ( S20 ) , Pseudomonas veronii ( S21 ) , and Sphingomonas trueperi ( S12 ) by sequencing of amplified 16S rRNA genes .
Strains S12 , S20 , and S21 contained the nitrogen fixation gene , nifD , but only S12 was able to reduce acetylene in pure culture .
The four strains significantly enhanced plant growth in the absence of pathogens , as evidenced by increases in plant height and dry weight of inoculated rice seedlings relative to noninoculated rice .
Three bacterial strains ( S3 , S20 , and S21 ) were evaluated in pot bioassays and reduced disease incidence by 50%-73% .
Strain S3 was as effective at suppressing disease at the lowest inoculum density ( 106 CFU/mL ) as at higher density ( 10 ( 8 ) CFU/mL or undiluted suspension ) .
This study indicates that selected endophytic bacterial strains have potential for control of seedling disease of rice and for plant growth promotion . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Four endophytic strains were highly effective against seedling disease in growth pouch assays , and these were identified as Pseudomonas fluorescens ( S3 ) , Pseudomonas tolaasii ( S20 ) , Pseudomonas veronii ( S21 ) , and Sphingomonas trueperi ( S12 ) by sequencing of amplified 16S rRNA genes .
[ Sen. 3, subscore: 2.00 ]: Strains S12 , S20 , and S21 contained the nitrogen fixation gene , nifD , but only S12 was able to reduce acetylene in pure culture .
[ Sen. 5, subscore: 2.00 ]: Three bacterial strains ( S3 , S20 , and S21 ) were evaluated in pot bioassays and reduced disease incidence by 50%-73% .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Molecular characterisation of segments 1 to 6 of Rice black-streaked dwarf virus from China provides the complete genome .
| | Author: Zhang HM Chen JP Adams MJ .
| | Journal: Arch . Virol . Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11811683 Accession (PMID): 11811683 | | Abstract: The nucleotide sequences of segments S1 to S6 of a Chinese isolate of Rice black-streaked dwarf virus ( RBSDV ) were determined .
This provides the first complete sequence of a plant pathogenic member of the genus Fijivirus .
The complete ten-segment genome has 29 , 141 nucleotides , making it the largest reovirus genome so far reported .
Each of the segments S1-S6 is predicted to encode a single major protein .
Protein comparisons indicated that S1 encoded an RNA dependent RNA polymerase , with similarities to that encoded by S1 of Nilaparvata lugens reovirus ( NLRV ) .
S2 and S3 appeared to be homologous to S3 and S4 respectively of both Fiji disease virus ( FDV ) and NLRV .
The protein encoded on S4 showed some similarity to that of NLRV S2 .
The proteins encoded on S5 and S6 , though similar in size to those of NLRV S5 and S6 , had no detectable homologies to them or to any other known protein . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: S2 and S3 appeared to be homologous to S3 and S4 respectively of both Fiji disease virus ( FDV ) and NLRV .
[ Sen. 7, subscore: 1.00 ]: The protein encoded on S4 showed some similarity to that of NLRV S2 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: An F-box gene linked to the self-incompatibility ( S ) locus of Antirrhinum is expressed specifically in pollen and tapetum .
| | Author: Lai Z Ma W Han B Liang L Zhang Y Hong G Xue Y | | Journal: Plant Mol . Biol . Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12139007 Accession (PMID): 12139007 | | Abstract: In many flowering plants , self-fertilization is prevented by an intraspecific reproductive barrier known as self-incompatibility ( SI ) , that , in most cases , is controlled by a single multiallelic S locus .
So far , the only known S locus product in self-incompatible species from the Solanaceae , Scrophulariaceae and Rosaceae is a class of ribonucleases called S RNases .
Molecular and transgenic analyses have shown that S RNases are responsible for pollen rejection by the pistil but have no role in pollen expression of SI , which appears to be mediated by a gene called the pollen self-incompatibility or Sp gene .
To identify possible candidates for this gene , we investigated the genomic structure of the S locus in Antirrhinum , a member of the Scrophulariaceae .
A novel F-box gene , AhSLF-S2 , encoded by the S2 allele , with the expected features of the Sp gene was identified .
AhSLF-S2 is located 9 kb downstream of S2 RNase gene and encodes a polypeptide of 376 amino acids with a conserved F-box domain in its amino-terminal part .
Hypothetical genes homologous to AhSLF-S2 are apparent in the sequenced genomic DNA of Arabidopsis and rice .
Together , they define a large gene family , named SLF ( S locus F-box ) family .
AhSLF-S2 is highly polymorphic and is specifically expressed in tapetum , microspores and pollen grains in an allele-specific manner .
The possibility that Sp encodes an F-box protein and the implications of this for the operation of self-incompatibility are discussed .
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[ Sen. 5, subscore: 1.00 ]: A novel F-box gene , AhSLF-S2 , encoded by the S2 allele , with the expected features of the Sp gene was identified .
[ Sen. 6, subscore: 1.00 ]: AhSLF-S2 is located 9 kb downstream of S2 RNase gene and encodes a polypeptide of 376 amino acids with a conserved F-box domain in its amino-terminal part .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
