| 29 matches found in 12 documents. Search time: 0.719 seconds. |
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| Title: Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes .
| | Author: Hayashi K Yoshida H Ashikawa I | | Journal: Year: 2006 | | Literature: oryza Field: abstract Doc ID: pub16673143 Accession (PMID): 16673143 | | Abstract: Blast resistance is one of the most important traits in rice breeding , and application of molecular markers for blast resistance breeding is likely to allow the rapid screening for the trait during early growth stages , without the need for inoculation of pathogen and phenotyping .
Allele-specific PCR markers and insertion/deletion ( InDel ) markers , which genotype single-nucleotide polymorphisms and InDel polymorphisms , respectively , are useful tools for marker-assisted selections .
We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib-which are commonly used in Japanese blast resistance rice breeding programs .
For each resistance gene , we used the segregation information from thousands of progeny in several crosses or published gene locations to generate a marker that cosegregated with the gene and markers that closely flanked the gene on either side .
The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
Therefore , these markers will likely facilitate the development of multiline cultivars carrying one or a combination of these nine blast resistance genes .
In addition , the systems we developed may be valuable tools in the quality control of seed production from blast-resistant multiline cultivars .
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[ Sen. 3, subscore: 2.00 ]: We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib-which are commonly used in Japanese blast resistance rice breeding programs .
[ Sen. 5, subscore: 2.00 ]: The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
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| Title: Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes .
| | Author: Hayashi K Yoshida H Ashikawa I | | Journal: Theor . Appl . Genet . Year: 2006 | | Literature: oryza Field: abstract Doc ID: pub16791691 Accession (PMID): 16791691 | | Abstract: Blast resistance is one of the most important traits in rice breeding , and application of molecular markers for blast resistance breeding is likely to allow the rapid screening for the trait during early growth stages , without the need for inoculation of pathogen and phenotyping .
Allele-specific PCR markers and insertion/deletion ( InDel ) markers , which genotype single-nucleotide polymorphisms and InDel polymorphisms , respectively , are useful tools for marker-assisted selections .
We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes -- Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib -- which are commonly used in Japanese blast resistance rice breeding programs .
For each resistance gene , we used the segregation information from thousands of progeny in several crosses or published gene locations to generate a marker that cosegregated with the gene and markers that closely flanked the gene on either side .
The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
Therefore , these markers will likely facilitate the development of multiline cultivars carrying one or a combination of these nine blast resistance genes .
In addition , the systems we developed may be valuable tools in the quality control of seed production from blast-resistant multiline cultivars . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes -- Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib -- which are commonly used in Japanese blast resistance rice breeding programs .
[ Sen. 5, subscore: 2.00 ]: The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The in silico map-based cloning of Pi36 , a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus .
| | Author: Liu X Lin F Wang L Pan Q | | Journal: Genetics Year: 2007 | | Literature: oryza Field: abstract Doc ID: pub17507669 Accession (PMID): 17507669 | | Abstract: The indica rice variety Kasalath carries Pi36 , a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8 .
The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance ( R ) gene content of the interval and hence for the identification of candidate gene ( s ) for Pi36 .
Three such sequences , which all had both a nucleotide-binding site and a leucine-rich repeat motif , were present .
The three candidate genes were amplified from the genomic DNA of a number of varieties by long-range PCR , and the resulting amplicons were inserted into pCAMBIA1300 and/or pYLTAC27 vectors to determine sequence polymorphisms correlated to the resistance phenotype and to perform transgenic complementation tests .
Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063 , which allowed the identification of Pi36-3 as the functional gene , with the other two candidates being probable pseudogenes .
The Pi36-encoded protein is composed of 1056 amino acids , with a single substitution event ( Asp to Ser ) at residue 590 associated with the resistant phenotype .
Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
An RT-PCR analysis showed that Pi36 is constitutively expressed in Kasalath .
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[ Sen. 7, subscore: 1.00 ]: Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1 .
| | Author: Lin F Chen S Que Z Wang L Liu X Pan Q | | Journal: Genetics Year: 2007 | | Literature: oryza Field: abstract Doc ID: pub17947408 Accession (PMID): 17947408 | | Abstract: The resistance ( R ) gene Pi37 , present in the rice cultivar St No 1 , was isolated by an in silico map-based cloning procedure .
The equivalent genetic region in Nipponbare contains four nucleotide binding site-leucine-rich repeat ( NBS-LRR ) type loci .
These four candidates for Pi37 ( Pi37-1 , -2 , -3 , and -4 ) were amplified separately from St No 1 via long-range PCR , and cloned into a binary vector .
Each construct was individually transformed into the highly blast susceptible cultivar Q1063 .
The subsequent complementation analysis revealed Pi37-3 to be the functional gene , while -1 , -2 , and -4 are probably pseudogenes .
Pi37 encodes a 1290 peptide NBS-LRR product , and the presence of substitutions at two sites in the NBS region ( V239A and I247M ) is associated with the resistance phenotype .
Semiquantitative expression analysis showed that in St No 1 , Pi37 was constitutively expressed and only slightly induced by blast infection .
Transient expression experiments indicated that the Pi37 product is restricted to the cytoplasm .
Pi37-3 is thought to have evolved recently from -2 , which in turn was derived from an ancestral -1 sequence .
Pi37-4 is likely the most recently evolved member of the cluster and probably represents a duplication of -3 .
The four Pi37 paralogs are more closely related to maize rp1 than to any of the currently isolated rice blast R genes Pita , Pib , Pi9 , Pi2 , Piz-t , and Pi36 .
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[ Sen. 11, subscore: 1.00 ]: The four Pi37 paralogs are more closely related to maize rp1 than to any of the currently isolated rice blast R genes Pita , Pib , Pi9 , Pi2 , Piz-t , and Pi36 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: [ Cloning and analyzing of rice blast resistance gene Pi-ta+ allele from Jinghong erect type of common wild rice ( Oryza rufipogon Griff ) in Yunnan ] | | Author: Geng XS Yang MZ Huang XQ Cheng ZQ Fu J Sun T Li J | | Journal: Yi Chuan Year: 2008 | | Literature: oryza Field: abstract Doc ID: pub18244911 Accession (PMID): 18244911 | | Abstract: A 4 , 672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast resistance gene Pi-ta+ has been cloned from Jinghong erect type of common wild rice ( Oryza rufipogon Griff ) in Yunnan by polymerase chain reaction method .
The coding region shares 99 . 86% and 98 . 78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively .
There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta+ gene , compared with Pi-ta from Yashiro-mochi .
Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele , because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA .
Pi-ta+ allele can cause disease resistance response to rice blast pathogens in plant cells .
Differences in DNA sequence , deduced amino acid sequence and antibacterial spectrum may make the Pi-ta+ allele new resistant characteristics .
Finding and cloning of Pi-ta+ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources .
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[ Sen. 4, subscore: 1.00 ]: Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele , because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
